Analytical biochemistry (Anal Biochem)

Journal PubWeight™ 19761.19‹?›

Top papers

Rank Title Year PubWeight™‹?›
1 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. 1976 903.81
2 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. 1987 281.19
3 A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. 1983 206.01
4 Measurement of protein using bicinchoninic acid. 1985 74.03
5 "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. 1981 71.15
6 "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. 1984 66.58
7 Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. 1987 59.79
8 A rapid boiling method for the preparation of bacterial plasmids. 1981 59.01
9 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. 1979 41.65
10 A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. 1978 38.54
11 A simplification of the protein assay method of Lowry et al. which is more generally applicable. 1977 36.95
12 A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. 1982 36.39
13 A modified uronic acid carbazole reaction. 1962 34.83
14 A rapid and mild procedure for the isolation of DNA from mammalian cells. 1982 34.01
15 A rapid, sensitive, and specific method for the determination of protein in dilute solution. 1973 31.32
16 Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. 1981 29.21
17 Calculation of protein extinction coefficients from amino acid sequence data. 1989 29.15
18 The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. 1996 28.76
19 Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. 1971 28.59
20 Ligand: a versatile computerized approach for characterization of ligand-binding systems. 1980 28.03
21 An evaluation of film detection methods for weak beta-emitters, particularly tritium. 1970 27.80
22 Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. 1982 27.26
23 New method for quantitative determination of uronic acids. 1973 27.02
24 Silver staining of proteins in polyacrylamide gels. 1981 26.33
25 A fractionating column for analysis of nucleic acids. 1960 26.31
26 Isolation of covalently closed circular DNA of high molecular weight from bacteria. 1976 25.02
27 ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. 1965 24.93
28 A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. 1980 24.76
29 A simple, rapid, and sensitive DNA assay procedure. 1980 23.88
30 A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. 1984 22.53
31 Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. 1979 22.03
32 Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. 1966 21.70
33 Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. 1976 21.07
34 A highly sensitive adenylate cyclase assay. 1974 20.13
35 Determination of protein: a modification of the Lowry method that gives a linear photometric response. 1972 19.75
36 A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. 1984 19.70
37 Universal chemical assay for the detection and determination of siderophores. 1987 18.60
38 Assay of proteins in the presence of interfering materials. 1976 17.90
39 Dideoxy sequencing method using denatured plasmid templates. 1986 17.34
40 Hybridization of nucleic acids immobilized on solid supports. 1984 17.32
41 Improved method for the isolation of RNA from plant tissues. 1987 16.74
42 A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. 1979 16.73
43 Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. 1991 16.18
44 A dot-immunobinding assay for monoclonal and other antibodies. 1982 15.84
45 Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. 1969 15.62
46 A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. 1981 15.38
47 The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. 1980 14.73
48 Semimicro determination of cellulose in biological materials. 1969 14.31
49 Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. 1980 14.14
50 A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. 1977 14.08
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