Biochemistry

Journal PubWeight™ 59473.10‹?›

Top papers

Rank Title Year PubWeight™‹?›
1 Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. 1979 180.95
2 Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. 1971 66.90
3 Enzymatic synthesis of deoxyribonucleic acid. XXXIV. Termination of chain growth by a 2',3'-dideoxyribonucleotide. 1969 60.64
4 Purine nucleosides. XII. The preparation of 2',3'-dideoxyadenosine, 2',5'-dideoxyadenosine, and 2',3',5'-trideoxyadenosine from 2'-deoxyadenosine. 1966 56.84
5 Synthesis of some nucleotides derived from 3'-deoxythymidine. 1969 56.71
6 Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. 1973 43.61
7 RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. 1977 38.82
8 Hydrogen ion buffers for biological research. 1966 30.95
9 Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. 1970 26.22
10 EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS. 1964 24.14
11 Effect of salts and polyamines on T4 polynucleotide kinase. 1975 22.01
12 Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. 1975 22.00
13 Spectroscopic determination of tryptophan and tyrosine in proteins. 1967 21.79
14 Reversal of bacteriophage T4 induced polynucleotide kinase action. 1973 21.41
15 Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. 1968 21.08
16 Prediction of protein conformation. 1974 20.51
17 Ribonucleic acid isolated by cesium chloride centrifugation. 1974 19.75
18 Dynamics of ligand binding to myoglobin. 1975 17.10
19 A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. 1975 16.43
20 Comparison of experimental binding data and theoretical models in proteins containing subunits. 1966 16.43
21 Dominant forces in protein folding. 1990 15.34
22 Deoxyribonucleic acid sequence organization in the mung bean genome. 1979 15.18
23 Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. 1968 14.82
24 A procedure for the isolation of mammalian messenger ribonucleic acid. 1972 14.47
25 Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation. 1994 13.94
26 The colchicine-binding protein of mammalian brain and its relation to microtubules. 1968 12.96
27 Conformational parameters for amino acids in helical, beta-sheet, and random coil regions calculated from proteins. 1974 12.07
28 Nucleotide sequence of the gene for human factor IX (antihemophilic factor B). 1985 11.80
29 New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. 1980 11.70
30 Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. 1974 11.67
31 VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. 1964 11.48
32 Structural identification of autoinducer of Photobacterium fischeri luciferase. 1981 11.48
33 Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. 1973 11.45
34 Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ. 1979 11.28
35 Rate of depurination of native deoxyribonucleic acid. 1972 10.85
36 Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. 1984 10.83
37 Computed circular dichroism spectra for the evaluation of protein conformation. 1969 10.58
38 Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease. 1989 10.50
39 Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate. 1988 10.17
40 DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS. 1964 10.04
41 PURIFICATION OF PHYTOCHROME FROM OAT SEEDLINGS. 1964 10.02
42 Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. 1967 10.01
43 The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions. Equilibria, kinetics, a suggested mechanism, and physiological implications. 1976 9.91
44 Thermodynamic parameters for an expanded nearest-neighbor model for formation of RNA duplexes with Watson-Crick base pairs. 1998 9.75
45 Mechanism of adenosine triphosphate hydrolysis by actomyosin. 1971 9.73
46 Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. 1988 9.66
47 The chemical shift index: a fast and simple method for the assignment of protein secondary structure through NMR spectroscopy. 1992 9.40
48 Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants. 1988 9.18
49 Estimation of globular protein secondary structure from circular dichroism. 1981 9.14
50 Diffusion-driven mechanisms of protein translocation on nucleic acids. 1. Models and theory. 1981 9.05
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