Gene

Journal PubWeight™ 18146.18‹?›

Top papers

Rank Title Year PubWeight™‹?›
1 Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. 1985 213.67
2 Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977 114.87
3 The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. 1982 102.30
4 Site-directed mutagenesis by overlap extension using the polymerase chain reaction. 1989 60.30
5 A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. 1982 59.01
6 Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. 1984 56.23
7 A simple and very efficient method for generating cDNA libraries. 1983 55.36
8 Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. 1988 52.71
9 Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. 1983 44.52
10 New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. 1988 42.65
11 CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. 1988 42.41
12 Efficient selection for high-expression transfectants with a novel eukaryotic vector. 1991 38.64
13 Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. 1979 25.53
14 A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. 1987 25.43
15 In vitro insertional mutagenesis with a selectable DNA fragment. 1984 25.21
16 Improved single and multicopy lac-based cloning vectors for protein and operon fusions. 1987 24.38
17 FACS-optimized mutants of the green fluorescent protein (GFP). 1996 24.12
18 Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. 1989 23.74
19 A small cosmid for efficient cloning of large DNA fragments. 1980 22.61
20 Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. 1982 20.39
21 A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. 1987 19.68
22 Multifunctional yeast high-copy-number shuttle vectors. 1992 19.47
23 Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. 1979 19.39
24 Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. 1995 18.98
25 The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. 1988 18.61
26 Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. 1988 18.10
27 Vectors for selective expression of cloned DNAs by T7 RNA polymerase. 1987 17.98
28 New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. 1984 17.36
29 Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. 1978 17.08
30 Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. 1979 16.95
31 Plasmid screening at high colony density. 1980 16.47
32 Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. 1995 15.67
33 A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. 1998 15.03
34 The making of strand-specific M13 probes. 1982 14.60
35 Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. 1991 14.15
36 Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. 1986 13.92
37 Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. 1995 13.86
38 Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. 1982 13.62
39 Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. 1994 13.42
40 Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. 1977 12.93
41 Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. 1981 12.78
42 Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. 1980 12.14
43 Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. 1982 11.99
44 Primary structure of the Aequorea victoria green-fluorescent protein. 1992 11.16
45 Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. 2001 11.09
46 Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. 1987 11.03
47 Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene. 1980 11.03
48 Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. 1980 10.77
49 In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1. 1977 10.75
50 High efficiency transformation of Escherichia coli with plasmids. 1990 10.65
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