Published in Trends Biotechnol on August 01, 1999
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Fast and economic immobilization methods described for non-commercial Pseudomonas lipases. BMC Biotechnol (2014) 0.86
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Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts. BMC Biotechnol (2012) 0.79
Voltammetry and in situ scanning tunneling microscopy of cytochrome C nitrite reductase on Au(111) electrodes. Biophys J (2006) 0.78
Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart. Biosci Rep (2016) 0.75
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The kinetics of phytochrome conversion. J Biol Chem (1966) 4.05
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Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active-site cleft. J Mol Biol (2000) 1.41
Purification and Properties of a Highly Thermostable, Sodium Dodecyl Sulfate-Resistant and Stereospecific Proteinase from the Extremely Thermophilic Archaeon Thermococcus stetteri. Appl Environ Microbiol (1995) 1.08
Improvement of posttranslational bottlenecks in the production of penicillin amidase in recombinant Escherichia coli strains. Appl Environ Microbiol (2003) 0.89
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Direct spectrophotometric measurement of enzyme activity in heterogeneous systems with insoluble substrate or immobilized enzyme. Anal Biochem (1994) 0.81
Catalytically active monomer forms of immobilized arginase. Eur J Biochem (1983) 0.81
Cooperative transitions between active -and -trypsin conformations. Biochem Biophys Res Commun (1972) 0.79
Direct measurement of pH profiles in immobilized enzyme carriers during kinetically controlled synthesis using CLSM. Biotechnol Prog (2001) 0.79
Resolution in affinity chromatography. The effect of the heterogeneity of immobilized soybean trypsin inhibitor on the separation of pancreatic proteases. J Chromatogr (1976) 0.78
Rapid zymogen activation and isolation of serine proteases from an individual mouse pancreas by affinity chromatography: genetical heterogeneity of chymotrypsins of Mus musculus. Biochim Biophys Acta (1977) 0.78
pH gradients in immobilized amidases and their influence on rates and yields of beta-lactam hydrolysis. Biotechnol Bioeng (1999) 0.78
Kinetic studies on the mechanism and the specificity of peptide semisynthesis catalyzed by the serine proteases alpha-chymotrypsin and beta-trypsin. Biochem Biophys Res Commun (1984) 0.77
A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP. Clin Genet (1985) 0.77
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Peptide synthesis catalyzed by the serine proteinases chymotrypsin and trypsin. Biochim Biophys Acta (1985) 0.76
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A theoretical model describing steady-state catalysis by enzymes immobilized in spherical gel particles. Experimental study of -chymotrypsin-sepharose. Biochem Biophys Res Commun (1971) 0.75
Oxygen effect: differences in radiation-induced deactivation of mobile and immobilized alpha-chymotrypsin. Radiat Environ Biophys (1974) 0.75
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A native, affinity-based protein blot for the analysis of streptavidin heterogeneity: consequences for the specificity of streptavidin mediated binding assays. Electrophoresis (1989) 0.75
The DNA, RNA and trypsin content in trypsins used in monolayer animal cell culture studies. Cell Biol Int Rep (1981) 0.75
Kinetically controlled semisynthesis of beta-lactam antibiotics and peptides. Ann N Y Acad Sci (1984) 0.75
An affinity chromatographic study of trypsinogen activation. Kinetic evidence for the existence of neotrypsinogen. Z Klin Chem Klin Biochem (1974) 0.75
Ionic strength and pH effects in the kinetically controlled synthesis of benzylpenicillin by nucleophilic deacylation of free and immobilized phenyl-acetyl-penicillin amidase with 6-aminopenicillanic acid. Biochem Biophys Res Commun (1982) 0.75
Tris(hydroxymethyl)methylamine is acylated when it reacts with acyl-chymotrypsin. Hoppe Seylers Z Physiol Chem (1982) 0.75
Evaluation of magnetic polymer micro-beads as carriers of immobilised biocatalysts for selective and stereoselective transformations. Biotechnol Lett (2004) 0.75
Kinetically controlled equilibria. The perturbation of hydrolysis equilibria in reactions catalyzed by alpha-chymotrypsin immobilized on charges supports. Biochem Biophys Res Commun (1978) 0.75
Conformational transitions in immobilized proteases. Methods Enzymol (1976) 0.75
Radical intermediates in the fluorescein- and eosin-photosensitized autoxidation of L-tyrosine. Photochem Photobiol (1967) 0.75
Activity distributions in modified alpha-chymotrypsin. Biochem Biophys Res Commun (1970) 0.75
Equilibrium and kinetically controlled synthesis with enzymes: semisynthesis of penicillins and peptides. Methods Enzymol (1987) 0.75
Crystallization of a precursor penicillin acylase from Escherichia coli. Acta Crystallogr D Biol Crystallogr (1999) 0.75
Purification of monoclonal antibodies by simulated moving-bed chromatography. J Chromatogr A (1997) 0.75
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The kinetics of trypsinogen activation studied by analytical affinity chromatography. Arch Biochem Biophys (1976) 0.75
Kinetic studies on the mechanism of the penicillin amidase-catalysed synthesis of ampicillin and benzylpenicillin. Hoppe Seylers Z Physiol Chem (1984) 0.75
A two step procedure for quantitative isolation of pure double strand DNA from animals tissues and cell cultures. Prep Biochem (1981) 0.75