Published in Protein Expr Purif on January 01, 2004
Localization of functional polypeptides in bacterial inclusion bodies. Appl Environ Microbiol (2006) 1.20
Refolding techniques for recovering biologically active recombinant proteins from inclusion bodies. Biomolecules (2014) 0.98
Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process. Microb Cell Fact (2015) 0.90
Effect of chemical chaperones in improving the solubility of recombinant proteins in Escherichia coli. Appl Environ Microbiol (2011) 0.86
L-arginine mediated renaturation enhances yield of human, α6 Type IV collagen non-collagenous domain from bacterial inclusion bodies. Protein Pept Lett (2012) 0.80
Purification and Refolding of Overexpressed Human Basic Fibroblast Growth Factor in Escherichia coli. Biotechnol Res Int (2011) 0.78
Production strategies for active heme-containing peroxidases from E. coli inclusion bodies - a review. Biotechnol Rep (Amst) (2016) 0.76
Targeting polyIC to EGFR over-expressing cells using a dsRNA binding protein domain tethered to EGF. PLoS One (2016) 0.75
REFOLDdb: a new and sustainable gateway to experimental protocols for protein refolding. BMC Struct Biol (2017) 0.75
An automatic refolding apparatus for preparative-scale protein production. PLoS One (2012) 0.75
Surface charge affects cellular uptake and intracellular trafficking of chitosan-based nanoparticles. Biomacromolecules (2011) 1.79
Preparation of hierarchical hollow CaCO3 particles and the application as anticancer drug carrier. J Am Chem Soc (2008) 1.14
Three-dimensional crystal structure and enzymic characterization of beta-mannanase Man5A from blue mussel Mytilus edulis. J Mol Biol (2006) 1.12
A thermosensitive hydrogel based on quaternized chitosan and poly(ethylene glycol) for nasal drug delivery system. Biomaterials (2007) 1.05
endo-beta-1,4-Mannanases from blue mussel, Mytilus edulis: purification, characterization, and mode of action. J Biotechnol (2002) 1.00
Preparation of uniform sized chitosan microspheres by membrane emulsification technique and application as a carrier of protein drug. J Control Release (2005) 0.96
PEGylation markedly enhances the in vivo potency of recombinant human non-glycosylated erythropoietin: a comparison with glycosylated erythropoietin. J Control Release (2010) 0.90
Preparation and characterization of uniform-sized chitosan microspheres containing insulin by membrane emulsification and a two-step solidification process. Colloids Surf B Biointerfaces (2006) 0.89
Influence of process parameters on the size distribution of PLA microcapsules prepared by combining membrane emulsification technique and double emulsion-solvent evaporation method. Colloids Surf B Biointerfaces (2005) 0.87
Preparation of uniform-sized PLA microcapsules by combining Shirasu porous glass membrane emulsification technique and multiple emulsion-solvent evaporation method. J Control Release (2004) 0.87
Preparation and improvement of release behavior of chitosan microspheres containing insulin. Int J Pharm (2006) 0.87
A thermo- and pH-sensitive hydrogel composed of quaternized chitosan/glycerophosphate. Int J Pharm (2006) 0.85
Chromatographic methods for the isolation of, and refolding of proteins from, Escherichia coli inclusion bodies. Protein Expr Purif (2002) 0.85
Preparation of insulin-loaded PLA/PLGA microcapsules by a novel membrane emulsification method and its release in vitro. Colloids Surf B Biointerfaces (2006) 0.84
Galactosylated nanocrystallites of insoluble anticancer drug for liver-targeting therapy: an in vitro evaluation. Nanomedicine (Lond) (2010) 0.83
Separation and purification of puerarin using beta-cyclodextrin-coupled agarose gel media. J Chromatogr A (2004) 0.83
Nanoparticles-based multi-adjuvant whole cell tumor vaccine for cancer immunotherapy. Biomaterials (2013) 0.82
A newly proposed mechanism for arginine-assisted protein refolding--not inhibiting soluble oligomers although promoting a correct structure. Protein Expr Purif (2006) 0.82
Poly (ethylene glycol) prodrug for anthracyclines via N-Mannich base linker: design, synthesis and biological evaluation. Int J Pharm (2009) 0.81
Crystallization and X-ray analysis of native and selenomethionyl beta-mannanase Man5A from blue mussel, Mytilus edulis, expressed in Pichia pastoris. Acta Crystallogr D Biol Crystallogr (2002) 0.81
Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification. J Colloid Interface Sci (2008) 0.81
Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration. Eur J Pharm Biopharm (2008) 0.81
Efficient preparation and PEGylation of recombinant human non-glycosylated erythropoietin expressed as inclusion body in E. coli. Int J Pharm (2009) 0.80
Cloning and expression in Pichia pastoris of a blue mussel (Mytilus edulis) beta-mannanase gene. Eur J Biochem (2002) 0.80
Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering. J Chromatogr A (2006) 0.80
Uniform-sized PLA nanoparticles: preparation by premix membrane emulsification. Int J Pharm (2008) 0.80
Hollow quaternized chitosan microspheres increase the therapeutic effect of orally administered insulin. Acta Biomater (2009) 0.79
Purification of the isoflavonoid puerarin by adsorption chromatography on cross-linked 12% agarose. J Chromatogr A (2004) 0.78
Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration. Bioresour Technol (2010) 0.78
Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci (2006) 0.78
Modeling of alphak/gamma2 (k=1, 2, 3 and 5) interface of GABA A receptor and docking studies with zolpidem: implications for selectivity. J Mol Graph Model (2007) 0.78
Molecular characterization of recombinant Hepatitis B surface antigen from Chinese hamster ovary and Hansenula polymorpha cells by high-performance size exclusion chromatography and multi-angle laser light scattering. J Chromatogr B Analyt Technol Biomed Life Sci (2006) 0.78
Double emulsion templated microcapsules with single hollow cavities and thickness-controllable shells. Langmuir (2009) 0.78
Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim. J Sep Sci (2012) 0.78
NMR studies on the interaction between (-)-epigallocatechin gallate and cyclodextrins, free and bonded to silica gels. Carbohydr Res (2007) 0.78
Novel vaccine delivery system induces robust humoral and cellular immune responses based on multiple mechanisms. Adv Healthc Mater (2013) 0.77
Preparation of uniform-sized pH-sensitive quaternized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method. Colloids Surf B Biointerfaces (2007) 0.77
Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures. Biotechnol Lett (2003) 0.77
Identification of an oxidative refolding intermediate of recombinant consensus interferon from inclusion bodies and design of a two-stage strategy to promote correct disulfide-bond formation. Biotechnol Appl Biochem (2007) 0.77
Preparation of uniform-sized agarose beads by microporous membrane emulsification technique. J Colloid Interface Sci (2007) 0.77
A mild hydrophobic interaction chromatography involving polyethylene glycol immobilized to agarose media refolding recombinant Staphylococcus aureus elongation factor G. Protein Expr Purif (2005) 0.76
[Influence of wall polymer and preparation process on the particle size and encapsulation of hemoglobin microcapsules]. Sheng Wu Gong Cheng Xue Bao (2004) 0.76
Conjugate of bovine hemoglobin and human serum albumin as a candidate for blood substitute: characteristics and effects on rats. Artif Cells Blood Substit Immobil Biotechnol (2005) 0.76
Salt induced irreversible protein adsorption with extremely high loadings on electrospun nanofibers. Langmuir (2010) 0.76
Hydrophobic interaction chromatography correctly refolding proteins assisted by glycerol and urea gradients. J Chromatogr A (2004) 0.75
Aryl ketones from Acronychia pedunculata with cyclooxygenase-2 inhibitory effects. Chem Biodivers (2005) 0.75
Single-step recovery of ephedrine hydrochloride from raw materials using expanded bed adsorption. Biotechnol Lett (2004) 0.75
Ion-exchange chromatography of hepatitis B virus surface antigen from a recombinant Chinese hamster ovary cell line. J Chromatogr A (2005) 0.75
[Separation of correctly refolded and mis-refolded consensus interferon by hydrophobic interaction chromatography]. Sheng Wu Gong Cheng Xue Bao (2005) 0.75
A cyanopropyl reversed phase chromatography enables resolution of huperzine A and B on analytical and preparative scales. J Sep Sci (2005) 0.75
Immobilized beta-cyclodextrin polymer coupled to agarose gel properly refolding recombinant Staphylococcus aureus elongation factor-G in combination with detergent micelle. Protein Expr Purif (2005) 0.75
One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media. J Chromatogr B Analyt Technol Biomed Life Sci (2005) 0.75
[Peptides analysis in digested edible bird's nest by HPLC-MS]. Zhongguo Zhong Yao Za Zhi (2013) 0.75
[Separation and purification of PEGylated rhG-CSF by two-step ion-exchange chromatography]. Sheng Wu Gong Cheng Xue Bao (2005) 0.75
Introduction to protein purification. Methods Biochem Anal (2011) 0.75
One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel. J Sep Sci (2011) 0.75
Separation of polyphenols using porous polyamide resin and assessment of mechanism of retention. J Sep Sci (2011) 0.75
[Purification and fingerprinting development of Salvia miltiorrhiza Bunge by high-speed counter-current chromatography]. Sheng Wu Gong Cheng Xue Bao (2003) 0.75
[Study on rhG-CSF modified with polyethylene glycol]. Sheng Wu Gong Cheng Xue Bao (2005) 0.75
Investigation of the adsorption behavior of glycine peptides on 12% cross-linked agarose gel media. J Chromatogr A (2010) 0.75
[Preparation of bovine lipid-free hemoglobin]. Sheng Wu Gong Cheng Xue Bao (2002) 0.75
LiCl-induced improvement of multilayer nanofibrous lipase for biodiesel synthesis. Bioresour Technol (2011) 0.75
An effective way to hydrophilize gigaporous polystyrene microspheres as rapid chromatographic separation media for proteins. Langmuir (2008) 0.75
Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography. Biotechnol J (2010) 0.75
Preparation and characterization of thermo-responsive albumin nanospheres. Int J Pharm (2007) 0.75
One-step rapid determination and purification of puerarin from Radix puerariae by n-octylamine-modified poly(methacrylate-co-ethylene dimethacrylate) monolith. J Chromatogr B Analyt Technol Biomed Life Sci (2008) 0.75
[Molecular imprinting-flow injection chemiluminescence method for determination of doxycycline hydrochloride]. Guang Pu Xue Yu Guang Pu Fen Xi (2009) 0.75
A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography. J Chromatogr A (2009) 0.75
Mixed-mode retention mechanism for (-)-epigallocatechin gallate on a 12% cross-linked agarose gel media. J Chromatogr A (2006) 0.75
[Comparison of adsorbent with varying arm length and ligand density for the purification of recombinant hepatitis B virus surface antigen]. Sheng Wu Gong Cheng Xue Bao (2007) 0.75
[Progress in purification of human serum albumin]. Sheng Wu Gong Cheng Xue Bao (2002) 0.75
[Simultaneous purification of superoxide dismutase, catalase and hemoglobin from bovine erythrocyte lysate with PEG 600 as chaperon]. Sheng Wu Gong Cheng Xue Bao (2005) 0.75
One-step purification of epigallocatechin gallate from crude green tea extracts by mixed-mode adsorption chromatography on highly cross-linked agarose media. J Chromatogr A (2007) 0.75
Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) (2002) 0.75
[Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology]. Sheng Wu Gong Cheng Xue Bao (2005) 0.75
Isolation of high purity 1-[2',4'-dihydroxy-3',5'-di-(3"-methylbut-2"-enyl)-6'-methoxy] phenylethanone from Acronychia pedunculata (L.) Miq. by high-speed counter-current chromatography. J Chromatogr A (2004) 0.75
[Development of a new hydrophobic interaction chromatography absorbent and its application to the purification of recombinant hepatitis B surface antigen]. Sheng Wu Gong Cheng Xue Bao (2006) 0.75
[Polyethylene glycol-accompanied ion-exchange chromatography to purify recombinant hepatitis B virus surface antigen]. Sheng Wu Gong Cheng Xue Bao (2005) 0.75
Affinity chromatography. Methods Biochem Anal (2011) 0.75
The preparation and characterization of monomethoxypoly(ethylene glycol)-b-poly-DL-lactide microcapsules containing bovine hemoglobin. Artif Cells Blood Substit Immobil Biotechnol (2003) 0.75
Novel poly(L-lysine) particles for gene delivery. J Control Release (2011) 0.75
[The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods]. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) (2003) 0.75