Synthetic shRNAs as potent RNAi triggers.

Characterization of Dicer-deficient murine embryonic stem cells. Proc Natl Acad Sci U S A (2005) 8.35

The promises and pitfalls of RNA-interference-based therapeutics. Nature (2009) 7.53

A dicer-independent miRNA biogenesis pathway that requires Ago catalysis. Nature (2010) 6.37

A pathway for the biogenesis of trans-acting siRNAs in Arabidopsis. Genes Dev (2005) 6.04

A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity. Science (2010) 5.84

RNAi therapeutics: principles, prospects and challenges. Adv Drug Deliv Rev (2007) 3.13

Functional polarity is introduced by Dicer processing of short substrate RNAs. Nucleic Acids Res (2005) 2.83

Aptamer mediated siRNA delivery. Nucleic Acids Res (2006) 2.77

The contributions of dsRNA structure to Dicer specificity and efficiency. RNA (2005) 2.48

Induction of the interferon response by siRNA is cell type- and duplex length-dependent. RNA (2006) 2.17

The loop position of shRNAs and pre-miRNAs is critical for the accuracy of dicer processing in vivo. Cell (2012) 1.84

RNAi and small interfering RNAs in human disease therapeutic applications. Trends Biotechnol (2010) 1.80

Semaphorin 4D provides a link between axon guidance processes and tumor-induced angiogenesis. Proc Natl Acad Sci U S A (2006) 1.76

Progress toward in vivo use of siRNAs-II. Mol Ther (2011) 1.60

A novel role of myosin VI in human prostate cancer. Am J Pathol (2006) 1.59

Artificial control of gene expression in mammalian cells by modulating RNA interference through aptamer-small molecule interaction. RNA (2006) 1.50

Specific residues at every third position of siRNA shape its efficient RNAi activity. Nucleic Acids Res (2007) 1.49

Defining the optimal parameters for hairpin-based knockdown constructs. RNA (2007) 1.48

Hydrophobization and bioconjugation for enhanced siRNA delivery and targeting. RNA (2007) 1.46

MicroRNA biogenesis: regulating the regulators. Crit Rev Biochem Mol Biol (2012) 1.41

RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs. Nucleic Acids Res (2006) 1.35

Effect of asymmetric terminal structures of short RNA duplexes on the RNA interference activity and strand selection. Nucleic Acids Res (2008) 1.21

Dicer-independent, Ago2-mediated microRNA biogenesis in vertebrates. Cell Cycle (2010) 1.21

RNA interference in biology and disease. Blood (2005) 1.18

Minimal-length short hairpin RNAs: the relationship of structure and RNAi activity. RNA (2009) 1.16

Sequence homology required by human immunodeficiency virus type 1 to escape from short interfering RNAs. J Virol (2006) 1.12

siRNA Versus miRNA as Therapeutics for Gene Silencing. Mol Ther Nucleic Acids (2015) 1.12

Sequence-non-specific effects of RNA interference triggers and microRNA regulators. Nucleic Acids Res (2009) 1.11

Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing. Nucleic Acids Res (2012) 1.07

Design of extended short hairpin RNAs for HIV-1 inhibition. Nucleic Acids Res (2007) 1.06

Dicer-independent processing of short hairpin RNAs. Nucleic Acids Res (2013) 1.06

Optimization of duplex stability and terminal asymmetry for shRNA design. PLoS One (2010) 1.05

Single small-interfering RNA expression vector for silencing multiple transforming growth factor-beta pathway components. Nucleic Acids Res (2005) 1.04

Suppression of prion protein in livestock by RNA interference. Proc Natl Acad Sci U S A (2006) 1.02

Effects of chemical modification on the potency, serum stability, and immunostimulatory properties of short shRNAs. RNA (2009) 1.02

The Business of RNAi Therapeutics in 2012. Mol Ther Nucleic Acids (2012) 1.01

The effects of stem length and core placement on shRNA activity. BMC Mol Biol (2011) 1.00

Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study. BMC Biotechnol (2007) 1.00

Recognition of siRNA asymmetry by TAR RNA binding protein. Biochemistry (2010) 1.00

Semaphorin 4D cooperates with VEGF to promote angiogenesis and tumor progression. Angiogenesis (2012) 0.99

Pol III Promoters to Express Small RNAs: Delineation of Transcription Initiation. Mol Ther Nucleic Acids (2014) 0.99

Salt intake augments hypotensive effects of transient receptor potential vanilloid 4: functional significance and implication. Hypertension (2008) 0.98

Sequence determinants of innate immune activation by short interfering RNAs. BMC Immunol (2009) 0.98

Designing Ago2-specific siRNA/shRNA to Avoid Competition with Endogenous miRNAs. Mol Ther Nucleic Acids (2014) 0.97

RNA interference in Entamoeba histolytica: implications for parasite biology and gene silencing. Future Microbiol (2011) 0.96

Chemical synthesis of a very long oligoribonucleotide with 2-cyanoethoxymethyl (CEM) as the 2'-O-protecting group: structural identification and biological activity of a synthetic 110mer precursor-microRNA candidate. Nucleic Acids Res (2007) 0.96

Development of Therapeutic-Grade Small Interfering RNAs by Chemical Engineering. Front Genet (2012) 0.95

ATP modulates siRNA interactions with an endogenous human Dicer complex. RNA (2005) 0.95

Synthetic pre-microRNAs reveal dual-strand activity of miR-34a on TNF-α. RNA (2013) 0.95

RNAimmuno: a database of the nonspecific immunological effects of RNA interference and microRNA reagents. RNA (2012) 0.94

Plexin-B1 activates NF-κB and IL-8 to promote a pro-angiogenic response in endothelial cells. PLoS One (2011) 0.94

siRNA efficiency: structure or sequence-that is the question. J Biomed Biotechnol (2006) 0.92

PKC{delta} is activated in a dietary model of steatohepatitis and regulates endoplasmic reticulum stress and cell death. J Biol Chem (2010) 0.90

Conditional Dicer substrate formation via shape and sequence transduction with small conditional RNAs. J Am Chem Soc (2013) 0.90

Complete, gene-specific siRNA libraries: production and expression in mammalian cells. RNA (2005) 0.90

Molecular basis for improved gene silencing by Dicer substrate interfering RNA compared with other siRNA variants. Nucleic Acids Res (2013) 0.88

Circular single-stranded synthetic DNA delivery vectors for microRNA. PLoS One (2011) 0.88

Gene silencing by RNAi in mouse Sertoli cells. Reprod Biol Endocrinol (2008) 0.88

Intensive RNAi with lentiviral vectors in mammalian cells. Methods (2008) 0.87

Development of a multipurpose GATEWAY-based lentiviral tetracycline-regulated conditional RNAi system (GLTR). PLoS One (2014) 0.87

Advances in Systemic siRNA Delivery. Drugs Future (2009) 0.87

Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing. Proc Natl Acad Sci U S A (2010) 0.86

In vitro binding of single-stranded RNA by human Dicer. FEBS Lett (2007) 0.86

The hypoxia-inducible factor-responsive proteins semaphorin 4D and vascular endothelial growth factor promote tumor growth and angiogenesis in oral squamous cell carcinoma. Exp Cell Res (2012) 0.86

Identification of GAS1 as an epirubicin resistance-related gene in human gastric cancer cells with a partially randomized small interfering RNA library. J Biol Chem (2009) 0.86

Deriving four functional anti-HIV siRNAs from a single Pol III-generated transcript comprising two adjacent long hairpin RNA precursors. Nucleic Acids Res (2010) 0.86

Embedding siRNA sequences targeting apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy. Mol Ther (2012) 0.86

Toxicity in mice expressing short hairpin RNAs gives new insight into RNAi. Genome Biol (2006) 0.86

RNA interference trigger variants: getting the most out of RNA for RNA interference-based therapeutics. Nucleic Acid Ther (2012) 0.86

RNA interference for improving the outcome of islet transplantation. Adv Drug Deliv Rev (2010) 0.84

Plexin-B1 and semaphorin 4D cooperate to promote perineural invasion in a RhoA/ROK-dependent manner. Am J Pathol (2012) 0.84

Pseudovirions as vehicles for the delivery of siRNA. Pharm Res (2009) 0.84

The Semaphorin 4D-Plexin-B1-RhoA signaling axis recruits pericytes and regulates vascular permeability through endothelial production of PDGF-B and ANGPTL4. Angiogenesis (2013) 0.83

Potent and systematic RNAi mediated silencing with single oligonucleotide compounds. RNA (2011) 0.83

Broad-spectrum antiviral activity of RNA interference against four genotypes of Japanese encephalitis virus based on single microRNA polycistrons. PLoS One (2011) 0.83

Native microRNA loop sequences can improve short hairpin RNA processing for virus gene silencing in animal cells. J RNAi Gene Silencing (2008) 0.83

In vivo therapeutic potential of Dicer-hunting siRNAs targeting infectious hepatitis C virus. Sci Rep (2014) 0.82

RNA interference of argininosuccinate synthetase restores sensitivity to recombinant arginine deiminase (rADI) in resistant cancer cells. J Biomed Sci (2011) 0.82

New insights into the promoterless transcription of DNA coligo templates by RNA polymerase III. Transcription (2014) 0.82

A random shRNA-encoding library for phenotypic selection and hit-optimization. PLoS One (2008) 0.82

Artificial mirtron-mediated gene knockdown: functional DMPK silencing in mammalian cells. RNA (2012) 0.82

Lentivirus mediated shRNA interference targeting MAT2B induces growth-inhibition and apoptosis in hepatocelluar carcinoma. World J Gastroenterol (2008) 0.81

A novel EST-derived RNAi screen reveals a critical role for farnesyl diphosphate synthase in β2-adrenergic receptor internalization and down-regulation. FASEB J (2012) 0.81

RNA interference approaches for treatment of HIV-1 infection. Genome Med (2015) 0.81

RNAi methodologies for the functional study of signaling molecules. PLoS One (2009) 0.81

Deep Sequencing Analyses of DsiRNAs Reveal the Influence of 3' Terminal Overhangs on Dicing Polarity, Strand Selectivity, and RNA Editing of siRNAs. Mol Ther Nucleic Acids (2012) 0.81

Rapid, controlled and intensive lentiviral vector-based RNAi. Methods Mol Biol (2009) 0.80

Endoribonuclease-prepared short interfering RNAs induce effective and specific inhibition of human immunodeficiency virus type 1 replication. J Virol (2007) 0.80

Dicing with siRNA. Nat Biotechnol (2005) 0.79

Reduced seed region-based off-target activity with lentivirus-mediated RNAi. RNA (2010) 0.79

Assessment of anti-scarring therapies in ex vivo organ cultured rabbit corneas. Exp Eye Res (2014) 0.79

Toward optimization of AgoshRNA molecules that use a non-canonical RNAi pathway: variations in the top and bottom base pairs. RNA Biol (2015) 0.79

Lentiviral vector-mediated RNA silencing in the central nervous system. Hum Gene Ther Methods (2013) 0.79

RNAi pathway integration in Caenorhabditis elegans development. Funct Integr Genomics (2011) 0.78

Effective inhibition of HIV-1 production by short hairpin RNAs and small interfering RNAs targeting a highly conserved site in HIV-1 Gag RNA is optimized by evaluating alternative length formats. Antimicrob Agents Chemother (2015) 0.78

Guidelines for the optimal design of miRNA-based shRNAs. Methods (2016) 0.78

Molecular Properties, Functional Mechanisms, and Applications of Sliced siRNA. Mol Ther Nucleic Acids (2015) 0.78

Semaphorin 4D Promotes Skeletal Metastasis in Breast Cancer. PLoS One (2016) 0.77

RNA interference in infectious tropical diseases. Korean J Parasitol (2008) 0.77

Gene targeting in primary human trophoblasts. Placenta (2012) 0.77

Gene expression profiling predicts clinical outcome of breast cancer. Nature (2002) 71.36

Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature (2005) 48.95

A microRNA polycistron as a potential human oncogene. Nature (2005) 40.21

MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet (2004) 37.83

Argonaute2 is the catalytic engine of mammalian RNAi. Science (2004) 22.86

Genetics of gene expression surveyed in maize, mouse and man. Nature (2003) 22.17

Genome-wide in situ exon capture for selective resequencing. Nat Genet (2007) 19.97

A microRNA component of the p53 tumour suppressor network. Nature (2007) 19.25

Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell (2007) 18.35

Expression profiling reveals off-target gene regulation by RNAi. Nat Biotechnol (2003) 16.63

Dicer is essential for mouse development. Nat Genet (2003) 15.87

Control of translation and mRNA degradation by miRNAs and siRNAs. Genes Dev (2006) 15.20

Processing of primary microRNAs by the Microprocessor complex. Nature (2004) 14.59

Identification and validation of oncogenes in liver cancer using an integrative oncogenomic approach. Cell (2006) 12.72

Crystal structure of Argonaute and its implications for RISC slicer activity. Science (2004) 12.48

MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat Cell Biol (2005) 12.12

Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures. Nature (2007) 11.66

Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence. Cell (2003) 11.40

Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes. Nature (2008) 10.71

Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev (2002) 10.71

A germline-specific class of small RNAs binds mammalian Piwi proteins. Nature (2006) 9.88

The Piwi-piRNA pathway provides an adaptive defense in the transposon arms race. Science (2007) 9.36

Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide. Cell (2008) 9.02

Probing tumor phenotypes using stable and regulated synthetic microRNA precursors. Nat Genet (2005) 8.99

Small RNAs as guardians of the genome. Cell (2009) 8.88

A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature (2004) 8.67

Characterization of Dicer-deficient murine embryonic stem cells. Proc Natl Acad Sci U S A (2005) 8.35

Second-generation shRNA libraries covering the mouse and human genomes. Nat Genet (2005) 8.23

An endogenous small interfering RNA pathway in Drosophila. Nature (2008) 7.88

Developmentally regulated piRNA clusters implicate MILI in transposon control. Science (2007) 7.68

MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev Cell (2007) 7.66

The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. Genes Dev (2002) 7.61

A role for the P-body component GW182 in microRNA function. Nat Cell Biol (2005) 7.54

Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity. RNA (2006) 7.06

The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Nat Struct Biol (2003) 6.90

Purified Argonaute2 and an siRNA form recombinant human RISC. Nat Struct Mol Biol (2005) 6.64

Fragile X-related protein and VIG associate with the RNA interference machinery. Genes Dev (2002) 6.43

Transcripts targeted by the microRNA-16 family cooperatively regulate cell cycle progression. Mol Cell Biol (2007) 6.43

Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary. Cell (2009) 6.39

A dicer-independent miRNA biogenesis pathway that requires Ago catalysis. Nature (2010) 6.37

A resource for large-scale RNA-interference-based screens in mammals. Nature (2004) 6.36

A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice. Mol Cell (2008) 6.35

A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish. Cell (2007) 6.31

CTLA-4 and PD-1 receptors inhibit T-cell activation by distinct mechanisms. Mol Cell Biol (2005) 6.18

Minimizing the risk of reporting false positives in large-scale RNAi screens. Nat Methods (2006) 5.96

A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity. Science (2010) 5.84

An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo. Nat Genet (2003) 5.82

Genome analysis of the platypus reveals unique signatures of evolution. Nature (2008) 5.74

Alta-Cyclic: a self-optimizing base caller for next-generation sequencing. Nat Methods (2008) 5.70

A genetic screen for candidate tumor suppressors identifies REST. Cell (2005) 5.61

An oncogenomics-based in vivo RNAi screen identifies tumor suppressors in liver cancer. Cell (2008) 5.47

Cancer proliferation gene discovery through functional genomics. Science (2008) 5.41

microRNAs join the p53 network--another piece in the tumour-suppression puzzle. Nat Rev Cancer (2007) 5.38

RNA interference in adult mice. Nature (2002) 5.33

A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc Natl Acad Sci U S A (2005) 5.29

MicroRNA 21 promotes glioma invasion by targeting matrix metalloproteinase regulators. Mol Cell Biol (2008) 5.21

A biochemical approach to identifying microRNA targets. Proc Natl Acad Sci U S A (2007) 5.16

Stable suppression of gene expression by RNAi in mammalian cells. Proc Natl Acad Sci U S A (2002) 5.05

An epigenetic role for maternally inherited piRNAs in transposon silencing. Science (2008) 5.05

A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii. Genes Dev (2007) 4.95

miR-19 is a key oncogenic component of mir-17-92. Genes Dev (2009) 4.95

Silencing of microRNA families by seed-targeting tiny LNAs. Nat Genet (2011) 4.81

MicroRNAs in the miR-106b family regulate p21/CDKN1A and promote cell cycle progression. Mol Cell Biol (2008) 4.76

Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing. RNA (2006) 4.72

Distinct catalytic and non-catalytic roles of ARGONAUTE4 in RNA-directed DNA methylation. Nature (2006) 4.68

The CRISPR system: small RNA-guided defense in bacteria and archaea. Mol Cell (2010) 4.60

Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation. Mol Cell (2009) 4.57

Profiling essential genes in human mammary cells by multiplex RNAi screening. Science (2008) 4.51

Hybrid selection of discrete genomic intervals on custom-designed microarrays for massively parallel sequencing. Nat Protoc (2009) 4.45

Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes. Genome Res (2007) 4.28

Small RNA sorting: matchmaking for Argonautes. Nat Rev Genet (2010) 4.27

Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application. Nat Rev Drug Discov (2010) 4.25

A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nat Methods (2011) 4.24

Critical roles for Dicer in the female germline. Genes Dev (2007) 4.23

Anaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activities. Nature (2007) 4.14

Rational development of LEA29Y (belatacept), a high-affinity variant of CTLA4-Ig with potent immunosuppressive properties. Am J Transplant (2005) 4.05

RNase III enzymes and the initiation of gene silencing. Nat Struct Mol Biol (2004) 4.01

Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure. Proc Natl Acad Sci U S A (2008) 4.00

Biochemical specialization within Arabidopsis RNA silencing pathways. Mol Cell (2005) 3.92

Wnt and TGF-beta signaling are required for the induction of an in vitro model of primitive streak formation using embryonic stem cells. Proc Natl Acad Sci U S A (2006) 3.85

miRNAs on the move: miRNA biogenesis and the RNAi machinery. Curr Opin Cell Biol (2004) 3.82

miR-146a is a significant brake on autoimmunity, myeloproliferation, and cancer in mice. J Exp Med (2011) 3.78

Gene expression changes associated with progression and response in chronic myeloid leukemia. Proc Natl Acad Sci U S A (2006) 3.70

A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell (2011) 3.69

A micrococcal nuclease homologue in RNAi effector complexes. Nature (2003) 3.67

The miRNA-processing enzyme dicer is essential for the morphogenesis and maintenance of hair follicles. Curr Biol (2006) 3.64