Solution structure of a calmodulin-target peptide complex by multidimensional NMR.

PubWeight™: 6.16‹?› | Rank: Top 1%

🔗 View Article (PMID 1585175)

Published in Science on May 01, 1992

Authors

M Ikura1, G M Clore, A M Gronenborn, G Zhu, C B Klee, A Bax

Author Affiliations

1: Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

Articles citing this

(truncated to the top 100)

Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc Natl Acad Sci U S A (2001) 6.63

Dynamic and quantitative Ca2+ measurements using improved cameleons. Proc Natl Acad Sci U S A (1999) 6.63

Structural analysis of substrate binding by the molecular chaperone DnaK. Science (1996) 6.13

Amino acid type determination in the sequential assignment procedure of uniformly 13C/15N-enriched proteins. J Biomol NMR (1993) 6.02

Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A (2004) 4.46

Proteopedia - a scientific 'wiki' bridging the rift between three-dimensional structure and function of biomacromolecules. Genome Biol (2008) 2.68

Calcium-dependent and -independent interactions of the S100 protein family. Biochem J (2006) 2.62

Cross-talk unfolded: MARCKS proteins. Biochem J (2002) 2.41

Genetically encoded Ca2+ indicators: using genetics and molecular design to understand complex physiology. J Physiol (2006) 2.12

Cloning and first functional characterization of a plant cyclic nucleotide-gated cation channel. Plant Physiol (1999) 2.06

Functional anthology of intrinsic disorder. 3. Ligands, post-translational modifications, and diseases associated with intrinsically disordered proteins. J Proteome Res (2007) 1.92

Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body compenents. J Cell Biol (1996) 1.92

A novel mechanism of PKA anchoring revealed by solution structures of anchoring complexes. EMBO J (2001) 1.75

Target recognition by calmodulin: dissecting the kinetics and affinity of interaction using short peptide sequences. Protein Sci (1996) 1.73

Modeling studies of the change in conformation required for cleavage of limited proteolytic sites. Protein Sci (1994) 1.63

Calcium-sensitive MRI contrast agents based on superparamagnetic iron oxide nanoparticles and calmodulin. Proc Natl Acad Sci U S A (2006) 1.63

Interaction with calmodulin is required for the function of Spc110p, an essential component of the yeast spindle pole body. EMBO J (1994) 1.60

Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein. Mol Cell Biol (2005) 1.58

Ca2+-independent phosphorylation of myosin in rat caudal artery and chicken gizzard myofilaments. J Physiol (1999) 1.58

Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands. Proc Natl Acad Sci U S A (1995) 1.57

Genetic polymorphism and protein conformational plasticity in the calmodulin superfamily: two ways to promote multifunctionality. Proc Natl Acad Sci U S A (2006) 1.54

The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast. Plant Physiol (2000) 1.54

Crystal structure of prokaryotic ribosomal protein L9: a bi-lobed RNA-binding protein. EMBO J (1994) 1.52

The energetic cost of domain reorientation in maltose-binding protein as studied by NMR and fluorescence spectroscopy. Proc Natl Acad Sci U S A (2003) 1.51

Harmonic and anharmonic aspects in the dynamics of BPTI: a normal mode analysis and principal component analysis. Protein Sci (1994) 1.51

NMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers. Proc Natl Acad Sci U S A (2013) 1.48

Cytoskeletal protein kinases: titin and its relations in mechanosensing. Pflugers Arch (2011) 1.46

Exploring the origins of binding specificity through the computational redesign of calmodulin. Proc Natl Acad Sci U S A (2003) 1.45

Experimentally exploring the conformational space sampled by domain reorientation in calmodulin. Proc Natl Acad Sci U S A (2004) 1.39

Structural basis for endothelial nitric oxide synthase binding to calmodulin. EMBO J (2003) 1.37

Recognition specificity of individual EH domains of mammals and yeast. EMBO J (1998) 1.36

A versatile amino acid analogue of the solvatochromic fluorophore 4-N,N-dimethylamino-1,8-naphthalimide: a powerful tool for the study of dynamic protein interactions. J Am Chem Soc (2008) 1.34

Signaling to myosin regulatory light chain in sarcomeres. J Biol Chem (2011) 1.33

Solution NMR structure of Apo-calmodulin in complex with the IQ motif of human cardiac sodium channel NaV1.5. J Mol Biol (2010) 1.32

Crystal structure of troponin C in complex with troponin I fragment at 2.3-A resolution. Proc Natl Acad Sci U S A (1998) 1.31

Study of conformational rearrangement and refinement of structural homology models by the use of heteronuclear dipolar couplings. J Biomol NMR (2000) 1.30

Quantification of the calcium-induced secondary structural changes in the regulatory domain of troponin-C. Protein Sci (1994) 1.30

Molecular and biochemical analysis of calmodulin interactions with the calmodulin-binding domain of plant glutamate decarboxylase. Plant Physiol (1995) 1.28

Structure and dynamics of calmodulin in solution. Biophys J (1998) 1.27

Giant protein kinases: domain interactions and structural basis of autoregulation. EMBO J (1996) 1.24

Crystal structure of a myristoylated CAP-23/NAP-22 N-terminal domain complexed with Ca2+/calmodulin. EMBO J (2004) 1.22

At-ACA8 encodes a plasma membrane-localized calcium-ATPase of Arabidopsis with a calmodulin-binding domain at the N terminus. Plant Physiol (2000) 1.22

Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin. EMBO J (2005) 1.22

DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell (2003) 1.21

Localization of calmodulin binding sites on the ryanodine receptor from skeletal muscle by electron microscopy. Biophys J (1994) 1.20

Calcium-calmodulin and regulation of brush border myosin-I MgATPase and mechanochemistry. J Cell Biol (1993) 1.17

Myosin light chain kinase and the role of myosin light chain phosphorylation in skeletal muscle. Arch Biochem Biophys (2011) 1.16

Signaling pathways underlying eosinophil cell motility revealed by using caged peptides. Proc Natl Acad Sci U S A (1998) 1.13

Fine structure analysis of the yeast centrin, Cdc31p, identifies residues specific for cell morphology and spindle pole body duplication. Genetics (2001) 1.13

Resonance energy transfer in a calcium concentration-dependent cameleon protein. Biophys J (2002) 1.12

Transient, sparsely populated compact states of apo and calcium-loaded calmodulin probed by paramagnetic relaxation enhancement: interplay of conformational selection and induced fit. J Am Chem Soc (2011) 1.11

Effect of amino acid substitutions on calmodulin binding and cytolytic properties of the LLP-1 peptide segment of human immunodeficiency virus type 1 transmembrane protein. J Virol (1995) 1.08

Oxidatively modified calmodulin binds to the plasma membrane Ca-ATPase in a nonproductive and conformationally disordered complex. Biophys J (2001) 1.07

Overlap between folding and functional energy landscapes for adenylate kinase conformational change. Nat Commun (2010) 1.07

Target sequence recognition by the calmodulin superfamily: implications from light chain binding to the regulatory domain of scallop myosin. Proc Natl Acad Sci U S A (1995) 1.07

Insights into modulation of calcium signaling by magnesium in calmodulin, troponin C and related EF-hand proteins. Biochim Biophys Acta (2011) 1.06

Calmodulin association with connexin32-derived peptides suggests trans-domain interaction in chemical gating of gap junction channels. J Biol Chem (2008) 1.06

StoneHinge: hinge prediction by network analysis of individual protein structures. Protein Sci (2009) 1.06

Quaternary structure built from subunits combining NMR and small-angle x-ray scattering data. Biophys J (2002) 1.06

Regulation of intrasteric inhibition of the multifunctional calcium/calmodulin-dependent protein kinase. Proc Natl Acad Sci U S A (1992) 1.05

Structure-based systematic isolation of conditional-lethal mutations in the single yeast calmodulin gene. Genetics (1994) 1.03

Wiggle-predicting functionally flexible regions from primary sequence. PLoS Comput Biol (2006) 1.03

Phenothiazines inhibit S100A4 function by inducing protein oligomerization. Proc Natl Acad Sci U S A (2010) 1.03

Structure of Paramecium tetraurelia calmodulin at 1.8 A resolution. Protein Sci (1993) 1.02

Fast photochemical oxidation of proteins for comparing structures of protein-ligand complexes: the calmodulin-peptide model system. Anal Chem (2010) 1.01

The vacuolar transporter chaperone (VTC) complex is required for microautophagy. Mol Biol Cell (2006) 1.01

Functional dynamics of the hydrophobic cleft in the N-domain of calmodulin. Biophys J (2001) 1.00

Protein conformational switches: from nature to design. Chemistry (2012) 0.99

Nef of HIV-1 interacts directly with calcium-bound calmodulin. Protein Sci (2002) 0.99

Regulation of c-Rel nuclear localization by binding of Ca2+/calmodulin. Mol Cell Biol (2003) 0.98

Sequence-specific determination of protein and peptide concentrations by absorbance at 205 nm. Protein Sci (2013) 0.98

The role of electrostatic interactions in calmodulin-peptide complex formation. Biophys J (2004) 0.98

Distinct properties of Ca2+-calmodulin binding to N- and C-terminal regulatory regions of the TRPV1 channel. J Gen Physiol (2012) 0.98

Solvent-induced differentiation of protein backbone hydrogen bonds in calmodulin. Protein Sci (2007) 0.97

Structural basis for calmodulin as a dynamic calcium sensor. Structure (2012) 0.97

Molecular and biochemical characterization of a calcium/calmodulin-binding protein kinase from rice. Biochem J (2002) 0.96

Distinct mechanisms of regulation by Ca2+/calmodulin of type 1 and 8 adenylyl cyclases support their different physiological roles. J Biol Chem (2008) 0.96

Activation of calcineurin and smooth muscle myosin light chain kinase by Met-to-Leu mutants of calmodulin. Biochem J (1998) 0.96

Protein structure elucidation from NMR proton densities. Proc Natl Acad Sci U S A (2002) 0.96

Activation-triggered subunit exchange between CaMKII holoenzymes facilitates the spread of kinase activity. Elife (2013) 0.96

A molecular dynamics study of Ca(2+)-calmodulin: evidence of interdomain coupling and structural collapse on the nanosecond timescale. Biophys J (2004) 0.96

Calmodulin tagging provides a general method of using lanthanide induced magnetic field orientation to observe residual dipolar couplings in proteins in solution. J Biomol NMR (2001) 0.96

Rational design of new binding specificity by simultaneous mutagenesis of calmodulin and a target peptide. Biochemistry (2006) 0.96

Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation. Biochemistry (2010) 0.95

Molecular determinants of the modulation of cyclic nucleotide-activated channels by calmodulin. Proc Natl Acad Sci U S A (1999) 0.95

Protein complexes studied by NMR spectroscopy. Curr Opin Biotechnol (1996) 0.95

Protein conformational changes studied by diffusion NMR spectroscopy: application to helix-loop-helix calcium binding proteins. Protein Sci (2003) 0.95

Crystal structure of calmodulin binding domain of orai1 in complex with Ca2+ calmodulin displays a unique binding mode. J Biol Chem (2012) 0.95

Molecular mechanism of the calcium-induced conformational change in the spectrin EF-hands. EMBO J (1995) 0.94

Identification of basic residues involved in activation and calmodulin binding of rabbit smooth muscle myosin light chain kinase. J Biol Chem (1992) 0.94

Calmodulin wraps around its binding domain in the plasma membrane Ca2+ pump anchored by a novel 18-1 motif. J Biol Chem (2009) 0.94

Dynamic light scattering study of calmodulin-target peptide complexes. Biophys J (2002) 0.94

Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters. Biophys Rev (2016) 0.94

The hypervariable region of K-Ras4B is responsible for its specific interactions with calmodulin. Biochemistry (2009) 0.94

Protein phi and psi dihedral restraints determined from multidimensional hypersurface correlations of backbone chemical shifts and their use in the determination of protein tertiary structures. J Biomol NMR (1997) 0.93

Chimeric myosin regulatory light chains identify the subdomain responsible for regulatory function. EMBO J (1992) 0.93

Yeast heat shock transcription factor N-terminal activation domains are unstructured as probed by heteronuclear NMR spectroscopy. Protein Sci (1996) 0.93

A rigid disulfide-linked nitroxide side chain simplifies the quantitative analysis of PRE data. J Biomol NMR (2011) 0.93

Both protein dynamics and ligand concentration can shift the binding mechanism between conformational selection and induced fit. Proc Natl Acad Sci U S A (2014) 0.92

Characterization of a mutant calcineurin A alpha gene expressed by EL4 lymphoma cells. Mol Cell Biol (1995) 0.92

Articles by these authors

Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr (1998) 169.28

NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR (1995) 93.94

Protein backbone angle restraints from searching a database for chemical shift and sequence homology. J Biomol NMR (1999) 26.99

B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med (1999) 16.91

Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease. Biochemistry (1989) 10.50

Recommendations for the presentation of NMR structures of proteins and nucleic acids. IUPAC-IUBMB-IUPAB Inter-Union Task Group on the Standardization of Data Bases of Protein and Nucleic Acid Structures Determined by NMR Spectroscopy. J Biomol NMR (1998) 9.65

Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium. Science (1997) 9.39

Amino acid type determination in the sequential assignment procedure of uniformly 13C/15N-enriched proteins. J Biomol NMR (1993) 6.02

A novel, highly stable fold of the immunoglobulin binding domain of streptococcal protein G. Science (1991) 6.00

Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra. J Magn Reson (1998) 5.83

Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta. Biochemistry (1989) 5.30

A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin. Biochemistry (1990) 5.01

Backbone dynamics of calmodulin studied by 15N relaxation using inverse detected two-dimensional NMR spectroscopy: the central helix is flexible. Biochemistry (1992) 4.25

Calmodulin. Annu Rev Biochem (1980) 4.22

B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol (2001) 4.10

Measurement of homo- and heteronuclear J couplings from quantitative J correlation. Methods Enzymol (1994) 3.97

Calmodulin. Adv Protein Chem (1982) 3.89

Demonstration of a spaser-based nanolaser. Nature (2009) 3.81

Three-dimensional solution structure of the 44 kDa ectodomain of SIV gp41. EMBO J (1998) 3.77

NMR structure of a specific DNA complex of Zn-containing DNA binding domain of GATA-1. Science (1993) 3.74

Determination of three-dimensional structures of proteins from interproton distance data by hybrid distance geometry-dynamical simulated annealing calculations. FEBS Lett (1988) 3.49

Multidimensional heteronuclear nuclear magnetic resonance of proteins. Methods Enzymol (1994) 3.45

Identification of the NH2-terminal blocking group of calcineurin B as myristic acid. FEBS Lett (1982) 3.42

The CD4 determinant for downregulation by HIV-1 Nef directly binds to Nef. Mapping of the Nef binding surface by NMR. Biochemistry (1996) 3.41

Solution structure of calcium-free calmodulin. Nat Struct Biol (1995) 3.36

Molecular basis of human 46X,Y sex reversal revealed from the three-dimensional solution structure of the human SRY-DNA complex. Cell (1995) 3.35

Calcineurin: a calcium- and calmodulin-binding protein of the nervous system. Proc Natl Acad Sci U S A (1979) 3.33

Determination of three-dimensional structures of proteins from interproton distance data by dynamical simulated annealing from a random array of atoms. Circumventing problems associated with folding. FEBS Lett (1988) 3.27

Solution structure of Ca(2+)-calmodulin reveals flexible hand-like properties of its domains. Nat Struct Biol (2001) 3.18

Inhibition of T cell signaling by immunophilin-ligand complexes correlates with loss of calcineurin phosphatase activity. Biochemistry (1992) 3.05

Demonstration of positionally disordered water within a protein hydrophobic cavity by NMR. Science (1995) 3.02

High-resolution structure of the oligomerization domain of p53 by multidimensional NMR. Science (1994) 2.98

Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A. Proc Natl Acad Sci U S A (1992) 2.98

The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase. Nat Struct Biol (1996) 2.94

Purification of cyclic 3',5'-nucleotide phosphodiesterase inhibitory protein by affinity chromatography on activator protein coupled to Sepharose. Biochemistry (1978) 2.89

Purification and characterization of smooth muscle myosin light chain kinase. J Biol Chem (1981) 2.84

Structures of larger proteins in solution: three- and four-dimensional heteronuclear NMR spectroscopy. Science (1991) 2.84

Solution structure of the N-terminal zinc binding domain of HIV-1 integrase. Nat Struct Biol (1997) 2.81

Determination of the three-dimensional solution structure of the C-terminal domain of cellobiohydrolase I from Trichoderma reesei. A study using nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing. Biochemistry (1989) 2.79

Refined solution structure of the oligomerization domain of the tumour suppressor p53. Nat Struct Biol (1995) 2.75

Discovery of a Ca2+- and calmodulin-dependent protein phosphatase: probable identity with calcineurin (CaM-BP80). FEBS Lett (1982) 2.75

Internal coordinates for molecular dynamics and minimization in structure determination and refinement. J Magn Reson (2001) 2.75

Determination of three-dimensional structures of proteins by simulated annealing with interproton distance restraints. Application to crambin, potato carboxypeptidase inhibitor and barley serine proteinase inhibitor 2. Protein Eng (1988) 2.74

Solution structure of the DNA binding domain of HIV-1 integrase. Biochemistry (1995) 2.69

The solution structure of a specific GAGA factor-DNA complex reveals a modular binding mode. Nat Struct Biol (1997) 2.67

A simple apparatus for generating stretched polyacrylamide gels, yielding uniform alignment of proteins and detergent micelles. J Biomol NMR (2001) 2.64

The solution structure of an HMG-I(Y)-DNA complex defines a new architectural minor groove binding motif. Nat Struct Biol (1997) 2.56

Changes in D(--)-beta-hydroxybutyric dehydrogenase activity during brain maturation in the rat. J Biol Chem (1967) 2.55

Use of dipolar 1H-15N and 1H-13C couplings in the structure determination of magnetically oriented macromolecules in solution. Nat Struct Biol (1997) 2.51

Measurement of HN-H alpha J couplings in calcium-free calmodulin using new 2D and 3D water-flip-back methods. J Biomol NMR (1994) 2.50

Flexibility and function in HIV-1 protease. Nat Struct Biol (1995) 2.49

Determining the structures of large proteins and protein complexes by NMR. Trends Biotechnol (1998) 2.49

Distinct functional and pharmacological properties of tonic and quantal inhibitory postsynaptic currents mediated by gamma-aminobutyric acid(A) receptors in hippocampal neurons. Mol Pharmacol (2001) 2.43

Recommendations for the presentation of NMR structures of proteins and nucleic acids. J Mol Biol (1998) 2.42

Identification of a binding site for the human immunodeficiency virus type 1 nucleocapsid protein. Proc Natl Acad Sci U S A (1993) 2.41

Direct structure refinement against residual dipolar couplings in the presence of rhombicity of unknown magnitude. J Magn Reson (1998) 2.40

Three-dimensional structure of interleukin 8 in solution. Biochemistry (1990) 2.40

Intercalation, DNA kinking, and the control of transcription. Science (1996) 2.39

A robust method for determining the magnitude of the fully asymmetric alignment tensor of oriented macromolecules in the absence of structural information. J Magn Reson (1998) 2.38

Analysis of the backbone dynamics of interleukin-1 beta using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy. Biochemistry (1990) 2.36

Conformational transition accompanying the binding of Ca2+ to the protein activator of 3',5'-cyclic adenosine monophosphate phosphodiesterase. Biochemistry (1977) 2.34

Phosphorylation of smooth muscle myosin light chain kinase by the catalytic subunit of adenosine 3': 5'-monophosphate-dependent protein kinase. J Biol Chem (1978) 2.33

Improving the quality of NMR and crystallographic protein structures by means of a conformational database potential derived from structure databases. Protein Sci (1996) 2.33

Improvements and extensions in the conformational database potential for the refinement of NMR and X-ray structures of proteins and nucleic acids. J Magn Reson (1997) 2.32

Assignment of the 31P and 1H resonances in oligonucleotides by two-dimensional NMR spectroscopy. FEBS Lett (1986) 2.28

Four-dimensional heteronuclear triple-resonance NMR spectroscopy of interleukin-1 beta in solution. Science (1990) 2.28

Solution structure of cyanovirin-N, a potent HIV-inactivating protein. Nat Struct Biol (1998) 2.19

Modulation of T-cell-mediated immunity in tumor and graft-versus-host disease models through the LIGHT co-stimulatory pathway. Nat Med (2000) 2.18

Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR. Protein Sci (1997) 2.16