Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations.

PubWeight™: 5.55‹?› | Rank: Top 1%

🔗 View Article (PMC 1276711)

Published in EMBO J on September 29, 2005

Authors

Scott B Hansen1, Gerlind Sulzenbacher, Tom Huxford, Pascale Marchot, Palmer Taylor, Yves Bourne

Author Affiliations

1: Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093-0636, USA.

Articles citing this

(truncated to the top 100)

Principles of activation and permeation in an anion-selective Cys-loop receptor. Nature (2011) 5.93

X-ray structure of a pentameric ligand-gated ion channel in an apparently open conformation. Nature (2008) 5.77

Mammalian nicotinic acetylcholine receptors: from structure to function. Physiol Rev (2009) 5.64

On the nature of partial agonism in the nicotinic receptor superfamily. Nature (2008) 4.18

Nicotinic receptors: allosteric transitions and therapeutic targets in the nervous system. Nat Rev Drug Discov (2009) 3.26

Detection and trapping of intermediate states priming nicotinic receptor channel opening. Nature (2009) 2.33

Crystal structure of a human GABAA receptor. Nature (2014) 2.22

Nicotine binding to brain receptors requires a strong cation-pi interaction. Nature (2009) 2.21

Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal alpha7 nicotinic acetylcholine receptor. EMBO J (2009) 1.91

The gating isomerization of neuromuscular acetylcholine receptors. J Physiol (2009) 1.84

Structural determinants of selective alpha-conotoxin binding to a nicotinic acetylcholine receptor homolog AChBP. Proc Natl Acad Sci U S A (2006) 1.78

X-ray structure of the mouse serotonin 5-HT3 receptor. Nature (2014) 1.67

Targeted molecular dynamics study of C-loop closure and channel gating in nicotinic receptors. PLoS Comput Biol (2006) 1.65

Ligand-binding domain of an α7-nicotinic receptor chimera and its complex with agonist. Nat Neurosci (2011) 1.58

Galanthamine and non-competitive inhibitor binding to ACh-binding protein: evidence for a binding site on non-alpha-subunit interfaces of heteromeric neuronal nicotinic receptors. J Mol Biol (2007) 1.54

Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism. Proc Natl Acad Sci U S A (2010) 1.48

X-ray structures of GluCl in apo states reveal a gating mechanism of Cys-loop receptors. Nature (2014) 1.46

Non-agonist-binding subunit interfaces confer distinct functional signatures to the alternate stoichiometries of the alpha4beta2 nicotinic receptor: an alpha4-alpha4 interface is required for Zn2+ potentiation. J Neurosci (2008) 1.43

Glycine receptor mechanism elucidated by electron cryo-microscopy. Nature (2015) 1.41

Additional acetylcholine (ACh) binding site at alpha4/alpha4 interface of (alpha4beta2)2alpha4 nicotinic receptor influences agonist sensitivity. J Biol Chem (2011) 1.39

Marine Macrocyclic Imines, Pinnatoxins A and G: Structural Determinants and Functional Properties to Distinguish Neuronal α7 from Muscle α1(2)βγδ nAChRs. Structure (2015) 1.39

Atomic structure and dynamics of pentameric ligand-gated ion channels: new insight from bacterial homologues. J Physiol (2009) 1.38

Acetylcholine receptor gating: movement in the alpha-subunit extracellular domain. J Gen Physiol (2007) 1.38

A structural and mutagenic blueprint for molecular recognition of strychnine and d-tubocurarine by different cys-loop receptors. PLoS Biol (2011) 1.38

Ligand activation of the prokaryotic pentameric ligand-gated ion channel ELIC. PLoS Biol (2011) 1.37

Identification of binding sites in the nicotinic acetylcholine receptor for TDBzl-etomidate, a photoreactive positive allosteric effector. J Biol Chem (2008) 1.36

Atomic interactions of neonicotinoid agonists with AChBP: molecular recognition of the distinctive electronegative pharmacophore. Proc Natl Acad Sci U S A (2008) 1.36

AChBP-targeted alpha-conotoxin correlates distinct binding orientations with nAChR subtype selectivity. EMBO J (2007) 1.35

Crystal structures of a pentameric ligand-gated ion channel provide a mechanism for activation. Proc Natl Acad Sci U S A (2013) 1.32

An ion selectivity filter in the extracellular domain of Cys-loop receptors reveals determinants for ion conductance. J Biol Chem (2008) 1.29

Cysteine modification reveals which subunits form the ligand binding site in human heteromeric 5-HT3AB receptors. J Physiol (2011) 1.29

Spectroscopic analysis of benzylidene anabaseine complexes with acetylcholine binding proteins as models for ligand-nicotinic receptor interactions. Biochemistry (2006) 1.28

Nanosecond-timescale conformational dynamics of the human alpha7 nicotinic acetylcholine receptor. Biophys J (2007) 1.28

A conserved salt bridge critical for GABA(A) receptor function and loop C dynamics. Proc Natl Acad Sci U S A (2008) 1.28

Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine. Nat Commun (2012) 1.26

Binding to gating transduction in nicotinic receptors: Cys-loop energetically couples to pre-M1 and M2-M3 regions. J Neurosci (2009) 1.25

Single-channel study of the spasmodic mutation alpha1A52S in recombinant rat glycine receptors. J Physiol (2007) 1.23

Alpha-conotoxins as pharmacological probes of nicotinic acetylcholine receptors. Acta Pharmacol Sin (2009) 1.22

Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors. Nat Chem (2012) 1.21

Nicotinic pharmacophore: the pyridine N of nicotine and carbonyl of acetylcholine hydrogen bond across a subunit interface to a backbone NH. Proc Natl Acad Sci U S A (2010) 1.18

Crystal structures of a cysteine-modified mutant in loop D of acetylcholine-binding protein. J Biol Chem (2010) 1.18

Gating of nicotinic ACh receptors; new insights into structural transitions triggered by agonist binding that induce channel opening. J Physiol (2007) 1.16

Structural requirements for eszopiclone and zolpidem binding to the gamma-aminobutyric acid type-A (GABAA) receptor are different. J Med Chem (2008) 1.14

An intersubunit hydrogen bond in the nicotinic acetylcholine receptor that contributes to channel gating. J Biol Chem (2008) 1.14

Merging old and new perspectives on nicotinic acetylcholine receptors. Biochem Pharmacol (2014) 1.14

Structure of the pentameric ligand-gated ion channel GLIC bound with anesthetic ketamine. Structure (2012) 1.14

Use of venom peptides to probe ion channel structure and function. J Biol Chem (2010) 1.14

Loop C and the mechanism of acetylcholine receptor-channel gating. J Gen Physiol (2013) 1.12

Alpha-conotoxin OmIA is a potent ligand for the acetylcholine-binding protein as well as alpha3beta2 and alpha7 nicotinic acetylcholine receptors. J Biol Chem (2006) 1.12

Chemical-scale studies on the role of a conserved aspartate in preorganizing the agonist binding site of the nicotinic acetylcholine receptor. Biochemistry (2007) 1.12

An integrated catch-and-hold mechanism activates nicotinic acetylcholine receptors. J Gen Physiol (2012) 1.11

Aromatic residues at position 55 of rat alpha7 nicotinic acetylcholine receptors are critical for maintaining rapid desensitization. J Physiol (2007) 1.11

Pharmacological characterization of the allosteric modulator desformylflustrabromine and its interaction with alpha4beta2 neuronal nicotinic acetylcholine receptor orthosteric ligands. J Pharmacol Exp Ther (2010) 1.11

Molecular-dynamics simulations of ELIC-a prokaryotic homologue of the nicotinic acetylcholine receptor. Biophys J (2009) 1.11

Nicotinic receptor interloop proline anchors beta1-beta2 and Cys loops in coupling agonist binding to channel gating. J Gen Physiol (2008) 1.10

In silico models for the human alpha4beta2 nicotinic acetylcholine receptor. J Phys Chem B (2008) 1.09

Conotoxins targeting nicotinic acetylcholine receptors: an overview. Mar Drugs (2014) 1.09

Gating of nicotinic ACh receptors: latest insights into ligand binding and function. J Physiol (2009) 1.08

Ligand-specific conformational changes in the alpha1 glycine receptor ligand-binding domain. J Biol Chem (2009) 1.08

Desensitization mechanism in prokaryotic ligand-gated ion channel. J Biol Chem (2012) 1.07

Atypical nicotinic agonist bound conformations conferring subtype selectivity. Proc Natl Acad Sci U S A (2008) 1.07

End-plate acetylcholine receptor: structure, mechanism, pharmacology, and disease. Physiol Rev (2012) 1.06

Spontaneous conformational change and toxin binding in alpha7 acetylcholine receptor: insight into channel activation and inhibition. Proc Natl Acad Sci U S A (2008) 1.05

Molecular actions of smoking cessation drugs at α4β2 nicotinic receptors defined in crystal structures of a homologous binding protein. Proc Natl Acad Sci U S A (2012) 1.04

The effective opening of nicotinic acetylcholine receptors with single agonist binding sites. J Gen Physiol (2011) 1.04

Single-channel current through nicotinic receptor produced by closure of binding site C-loop. Biophys J (2009) 1.04

Intramembrane proton binding site linked to activation of bacterial pentameric ion channel. J Biol Chem (2011) 1.03

Structural rearrangements in loop F of the GABA receptor signal ligand binding, not channel activation. Biophys J (2009) 1.03

Alpha-RgIA, a novel conotoxin that blocks the alpha9alpha10 nAChR: structure and identification of key receptor-binding residues. J Mol Biol (2008) 1.02

Intersubunit bridge formation governs agonist efficacy at nicotinic acetylcholine α4β2 receptors: unique role of halogen bonding revealed. J Biol Chem (2011) 1.02

A unified model of the GABA(A) receptor comprising agonist and benzodiazepine binding sites. PLoS One (2013) 1.02

Structural model of rho1 GABAC receptor based on evolutionary analysis: Testing of predicted protein-protein interactions involved in receptor assembly and function. Protein Sci (2009) 1.01

Inter-subunit disulfide cross-linking in homomeric and heteromeric P2X receptors. Eur Biophys J (2008) 1.01

An allosteric modulator of alpha7 nicotinic receptors, N-(5-Chloro-2,4-dimethoxyphenyl)-N'-(5-methyl-3-isoxazolyl)-urea (PNU-120596), causes conformational changes in the extracellular ligand binding domain similar to those caused by acetylcholine. Mol Pharmacol (2009) 1.00

Structure-guided drug design: conferring selectivity among neuronal nicotinic receptor and acetylcholine-binding protein subtypes. Biochem Pharmacol (2007) 1.00

Epibatidine binds to four sites on the Torpedo nicotinic acetylcholine receptor. Biochem Biophys Res Commun (2007) 1.00

Amino acid residues that confer high selectivity of the alpha6 nicotinic acetylcholine receptor subunit to alpha-conotoxin MII[S4A,E11A,L15A]. J Biol Chem (2008) 0.99

Myasthenia gravis and the tops and bottoms of AChRs: antigenic structure of the MIR and specific immunosuppression of EAMG using AChR cytoplasmic domains. Ann N Y Acad Sci (2008) 0.99

Main immunogenic region structure promotes binding of conformation-dependent myasthenia gravis autoantibodies, nicotinic acetylcholine receptor conformation maturation, and agonist sensitivity. J Neurosci (2009) 0.99

Generation of candidate ligands for nicotinic acetylcholine receptors via in situ click chemistry with a soluble acetylcholine binding protein template. J Am Chem Soc (2012) 0.99

Magnitude of a conformational change in the glycine receptor beta1-beta2 loop is correlated with agonist efficacy. J Biol Chem (2009) 0.99

Effect of novel negative allosteric modulators of neuronal nicotinic receptors on cells expressing native and recombinant nicotinic receptors: implications for drug discovery. J Pharmacol Exp Ther (2008) 0.98

Synaptic neurotransmitter-gated receptors. Cold Spring Harb Perspect Biol (2012) 0.98

Crystal structure of Lymnaea stagnalis AChBP complexed with the potent nAChR antagonist DHβE suggests a unique mode of antagonism. PLoS One (2012) 0.98

Cysteine accessibility analysis of the human alpha7 nicotinic acetylcholine receptor ligand-binding domain identifies L119 as a gatekeeper. Neuropharmacology (2010) 0.98

Molecular mechanisms of Cys-loop ion channel receptor modulation by ivermectin. Front Mol Neurosci (2012) 0.98

Structural characterization of binding mode of smoking cessation drugs to nicotinic acetylcholine receptors through study of ligand complexes with acetylcholine-binding protein. J Biol Chem (2012) 0.98

Molecular determinants for competitive inhibition of alpha4beta2 nicotinic acetylcholine receptors. Mol Pharmacol (2010) 0.97

Molecular basis for the differential sensitivity of rat and human α9α10 nAChRs to α-conotoxin RgIA. J Neurochem (2012) 0.97

Atypical alpha-conotoxin LtIA from Conus litteratus targets a novel microsite of the alpha3beta2 nicotinic receptor. J Biol Chem (2010) 0.97

NMR structures of the transmembrane domains of the α4β2 nAChR. Biochim Biophys Acta (2012) 0.97

Defining nicotinic agonist binding surfaces through photoaffinity labeling. Biochemistry (2007) 0.96

Agonist- and antagonist-induced conformational changes of loop F and their contributions to the rho1 GABA receptor function. J Physiol (2008) 0.96

Blockade of neuronal α7-nAChR by α-conotoxin ImI explained by computational scanning and energy calculations. PLoS Comput Biol (2011) 0.96

Myasthenic syndrome AChRα C-loop mutant disrupts initiation of channel gating. J Clin Invest (2012) 0.95

Creating an α7 nicotinic acetylcholine recognition domain from the acetylcholine-binding protein: crystallographic and ligand selectivity analyses. J Biol Chem (2011) 0.95

Mapping the elusive neonicotinoid binding site. Proc Natl Acad Sci U S A (2007) 0.94

Physostigmine and galanthamine bind in the presence of agonist at the canonical and noncanonical subunit interfaces of a nicotinic acetylcholine receptor. J Neurosci (2013) 0.93

The neurobiologist's guide to structural biology: a primer on why macromolecular structure matters and how to evaluate structural data. Neuron (2007) 0.93

Structural answers and persistent questions about how nicotinic receptors work. Front Biosci (2008) 0.93

Articles cited by this

The CCP4 suite: programs for protein crystallography. Acta Crystallogr D Biol Crystallogr (1994) 187.88

Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr (1997) 137.43

Automated protein model building combined with iterative structure refinement. Nat Struct Biol (1999) 27.52

Refined structure of the nicotinic acetylcholine receptor at 4A resolution. J Mol Biol (2005) 12.16

Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors. Nature (2001) 11.78

Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures. Neuron (2004) 7.48

Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line. Proc Natl Acad Sci U S A (2002) 5.91

Emerging structure of the nicotinic acetylcholine receptors. Nat Rev Neurosci (2002) 5.87

A glia-derived acetylcholine-binding protein that modulates synaptic transmission. Nature (2001) 3.69

Cys-loop receptors: new twists and turns. Trends Neurosci (2004) 3.58

Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors. EMBO J (2005) 2.91

Coupling of agonist binding to channel gating in an ACh-binding protein linked to an ion channel. Nature (2004) 2.77

Crystal structure of nicotinic acetylcholine receptor homolog AChBP in complex with an alpha-conotoxin PnIA variant. Nat Struct Mol Biol (2005) 2.45

Treatment of autoimmune myasthenia gravis. Neurology (2003) 2.20

Structural and ligand recognition characteristics of an acetylcholine-binding protein from Aplysia californica. J Biol Chem (2004) 1.96

Snake venom alpha-neurotoxins and other 'three-finger' proteins. Eur J Biochem (1999) 1.83

Tryptophan fluorescence reveals conformational changes in the acetylcholine binding protein. J Biol Chem (2002) 1.81

A medium-throughput crystallization approach. Acta Crystallogr D Biol Crystallogr (2002) 1.72

Nicotinic receptors in wonderland. Trends Biochem Sci (2001) 1.67

Methyllycaconitine: a selective probe for neuronal alpha-bungarotoxin binding sites. FEBS Lett (1990) 1.54

Lobeline: structure-affinity investigation of nicotinic acetylcholinergic receptor binding. J Med Chem (1999) 1.53

Comparative pharmacology of epibatidine: a potent agonist for neuronal nicotinic acetylcholine receptors. Mol Pharmacol (1995) 1.49

Epibatidine, a potent analgetic and nicotinic agonist. Mol Pharmacol (1994) 1.44

Alpha-conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes. Eur J Biochem (2004) 1.23

Lobeline and structurally simplified analogs exhibit differential agonist activity and sensitivity to antagonist blockade when compared to nicotine. Neuropharmacology (1998) 1.17

Alpha-conotoxins ImI and ImII. Similar alpha 7 nicotinic receptor antagonists act at different sites. J Biol Chem (2002) 1.15

Alpha-conotoxins ImI and ImII target distinct regions of the human alpha7 nicotinic acetylcholine receptor and distinguish human nicotinic receptor subtypes. Biochemistry (2004) 1.13

Structural elements in alpha-conotoxin ImI essential for binding to neuronal alpha7 receptors. J Biol Chem (1998) 1.10

NMR spatial structure of alpha-conotoxin ImI reveals a common scaffold in snail and snake toxins recognizing neuronal nicotinic acetylcholine receptors. FEBS Lett (1999) 1.09

NMR solution structure of alpha-conotoxin ImI and comparison to other conotoxins specific for neuronal nicotinic acetylcholine receptors. Biochemistry (1999) 1.04

Relations between structure and nicotine-like activity: X-ray crystal structure analysis of (-)-cytisine and (-)-lobeline hydrochloride and a comparison with (-)-nicotine and other nicotine-like compounds. Br J Pharmacol (1989) 1.01

Structure activity studies of ring E analogues of methyllycaconitine. Part 2: Synthesis of antagonists to the alpha3beta4* nicotinic acetylcholine receptors through modifications to the ester. Bioorg Med Chem Lett (2004) 1.01

Chemical modification of the active site of the acetylcholine receptor. J Gen Physiol (1969) 1.00

Functional determinants by which snake and cone snail toxins block the alpha 7 neuronal nicotinic acetylcholine receptors. J Physiol Paris (1998) 0.96

Elementary steps in synaptic transmission revealed by currents through single ion channels. Science (1992) 0.95

Conversion of the sodium channel activator aconitine into a potent alpha 7-selective nicotinic ligand. FEBS Lett (1995) 0.90

Articles by these authors

A splice code for trans-synaptic cell adhesion mediated by binding of neuroligin 1 to alpha- and beta-neurexins. Neuron (2005) 3.93

Dissection of synapse induction by neuroligins: effect of a neuroligin mutation associated with autism. J Biol Chem (2005) 3.06

Click chemistry in situ: acetylcholinesterase as a reaction vessel for the selective assembly of a femtomolar inhibitor from an array of building blocks. Angew Chem Int Ed Engl (2002) 3.06

Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors. EMBO J (2005) 2.91

Coupling of agonist binding to channel gating in an ACh-binding protein linked to an ion channel. Nature (2004) 2.77

The Arg451Cys-neuroligin-3 mutation associated with autism reveals a defect in protein processing. J Neurosci (2004) 2.66

LRRTM2 interacts with Neurexin1 and regulates excitatory synapse formation. Neuron (2009) 2.60

Bacterial glycosidases for the production of universal red blood cells. Nat Biotechnol (2007) 2.41

Crystal structure of the RNA polymerase domain of the West Nile virus non-structural protein 5. J Biol Chem (2007) 2.28

Structural analysis of the synaptic protein neuroligin and its beta-neurexin complex: determinants for folding and cell adhesion. Neuron (2007) 2.04

Structural and ligand recognition characteristics of an acetylcholine-binding protein from Aplysia californica. J Biol Chem (2004) 1.96

Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal alpha7 nicotinic acetylcholine receptor. EMBO J (2009) 1.91

Biophysical characterization of the free IkappaBalpha ankyrin repeat domain in solution. Protein Sci (2004) 1.89

ESTHER, the database of the α/β-hydrolase fold superfamily of proteins: tools to explore diversity of functions. Nucleic Acids Res (2012) 1.88

Tryptophan fluorescence reveals conformational changes in the acetylcholine binding protein. J Biol Chem (2002) 1.81

Agonist-mediated conformational changes in acetylcholine-binding protein revealed by simulation and intrinsic tryptophan fluorescence. J Biol Chem (2004) 1.81

A medium-throughput crystallization approach. Acta Crystallogr D Biol Crystallogr (2002) 1.72

Gene selection, alternative splicing, and post-translational processing regulate neuroligin selectivity for beta-neurexins. Biochemistry (2006) 1.67

The three-dimensional structure of invertase (beta-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases. J Biol Chem (2004) 1.64

In situ click chemistry: enzyme inhibitors made to their own specifications. J Am Chem Soc (2004) 1.61

Thermodynamics reveal that helix four in the NLS of NF-kappaB p65 anchors IkappaBalpha, forming a very stable complex. J Mol Biol (2006) 1.59

Galanthamine and non-competitive inhibitor binding to ACh-binding protein: evidence for a binding site on non-alpha-subunit interfaces of heteromeric neuronal nicotinic receptors. J Mol Biol (2007) 1.54

Medium-scale structural genomics: strategies for protein expression and crystallization. Acc Chem Res (2003) 1.53

Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic site. EMBO J (2003) 1.53

ESTHER, the database of the alpha/beta-hydrolase fold superfamily of proteins. Nucleic Acids Res (2004) 1.52

Freeze-frame inhibitor captures acetylcholinesterase in a unique conformation. Proc Natl Acad Sci U S A (2004) 1.52

Control of cation permeation through the nicotinic receptor channel. PLoS Comput Biol (2008) 1.51

Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism. Proc Natl Acad Sci U S A (2010) 1.48

In situ selection of lead compounds by click chemistry: target-guided optimization of acetylcholinesterase inhibitors. J Am Chem Soc (2005) 1.47

Aspergillus niger protein EstA defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily of proteins. Structure (2004) 1.46

Characterization of the interaction of a recombinant soluble neuroligin-1 with neurexin-1beta. J Biol Chem (2003) 1.46

The crystal structure of the α-neurexin-1 extracellular region reveals a hinge point for mediating synaptic adhesion and function. Structure (2011) 1.39

Atomic interactions of neonicotinoid agonists with AChBP: molecular recognition of the distinctive electronegative pharmacophore. Proc Natl Acad Sci U S A (2008) 1.36

Tetrameric mouse acetylcholinesterase: continuum diffusion rate calculations by solving the steady-state Smoluchowski equation using finite element methods. Biophys J (2004) 1.35

X-ray crystal structure of an IkappaBbeta x NF-kappaB p65 homodimer complex. J Biol Chem (2003) 1.35

Synaptic arrangement of the neuroligin/beta-neurexin complex revealed by X-ray and neutron scattering. Structure (2007) 1.34

Mutant cholinesterases possessing enhanced capacity for reactivation of their phosphonylated conjugates. Biochemistry (2004) 1.31

LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosis. EMBO J (2006) 1.31

Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding. J Biol Chem (2006) 1.29

A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the alpha,beta-hydrolase fold protein family. J Biol Chem (2006) 1.29

An ion selectivity filter in the extracellular domain of Cys-loop receptors reveals determinants for ion conductance. J Biol Chem (2008) 1.29

Spectroscopic analysis of benzylidene anabaseine complexes with acetylcholine binding proteins as models for ligand-nicotinic receptor interactions. Biochemistry (2006) 1.28

Current progress on esterases: from molecular structure to function. Drug Metab Dispos (2002) 1.24

High-throughput automated refolding screening of inclusion bodies. Protein Sci (2004) 1.23

Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis. A novel carboxyl esterase structurally related to the HSL family. Eur J Biochem (2004) 1.20

Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis. J Biol Chem (2004) 1.20

Ligand-induced conformational changes in the acetylcholine-binding protein analyzed by hydrogen-deuterium exchange mass spectrometry. J Biol Chem (2006) 1.20

Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion. Endocrinology (2008) 1.19

Refinement of structural leads for centrally acting oxime reactivators of phosphylated cholinesterases. J Biol Chem (2012) 1.18

Mechanism of acetylcholinesterase inhibition by fasciculin: a 5-ns molecular dynamics simulation. J Am Chem Soc (2002) 1.17

Ligand-induced conformational change in the alpha7 nicotinic receptor ligand binding domain. Biophys J (2005) 1.17

Asymmetric structural motions of the homomeric alpha7 nicotinic receptor ligand binding domain revealed by molecular dynamics simulation. Biophys J (2003) 1.17

Influence of agonists and antagonists on the segmental motion of residues near the agonist binding pocket of the acetylcholine-binding protein. J Biol Chem (2006) 1.17

New structural scaffolds for centrally acting oxime reactivators of phosphylated cholinesterases. J Biol Chem (2011) 1.14

Acetylcholinesterase active centre and gorge conformations analysed by combinatorial mutations and enantiomeric phosphonates. Biochem J (2003) 1.14

Acrylodan-conjugated cysteine side chains reveal conformational state and ligand site locations of the acetylcholine-binding protein. J Biol Chem (2004) 1.12

Alpha-conotoxin OmIA is a potent ligand for the acetylcholine-binding protein as well as alpha3beta2 and alpha7 nicotinic acetylcholine receptors. J Biol Chem (2006) 1.12

Atypical nicotinic agonist bound conformations conferring subtype selectivity. Proc Natl Acad Sci U S A (2008) 1.07

Identification of a GH110 subfamily of alpha 1,3-galactosidases: novel enzymes for removal of the alpha 3Gal xenotransplantation antigen. J Biol Chem (2008) 1.07

Characterizing ligand-gated ion channel receptors with genetically encoded Ca2++ sensors. PLoS One (2011) 1.06

Nanosecond dynamics of the mouse acetylcholinesterase cys69-cys96 omega loop. J Biol Chem (2003) 1.06

Targeting of acetylcholinesterase in neurons in vivo: a dual processing function for the proline-rich membrane anchor subunit and the attachment domain on the catalytic subunit. J Neurosci (2009) 1.04

Crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosis. FEBS Lett (2005) 1.04

The nuclear I kappaB protein I kappaB zeta specifically binds NF-kappaB p50 homodimers and forms a ternary complex on kappaB DNA. J Mol Biol (2008) 1.04

Curariform antagonists bind in different orientations to acetylcholine-binding protein. J Biol Chem (2003) 1.04

Butyrylcholinesterase: association with the metabolic syndrome and identification of 2 gene loci affecting activity. Clin Chem (2006) 1.03

Molecular mimicry of the NF-kappaB DNA target site by a selected RNA aptamer. Curr Opin Struct Biol (2004) 1.02

A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. PLoS Biol (2013) 1.01

Oxime-assisted acetylcholinesterase catalytic scavengers of organophosphates that resist aging. J Biol Chem (2011) 1.01

Solution NMR of acetylcholine binding protein reveals agonist-mediated conformational change of the C-loop. Mol Pharmacol (2006) 0.99

Pharm. D. pathways to biomedical research: the National Institutes of Health special conference on pharmacy research. Pharmacotherapy (2008) 0.99

Main immunogenic region structure promotes binding of conformation-dependent myasthenia gravis autoantibodies, nicotinic acetylcholine receptor conformation maturation, and agonist sensitivity. J Neurosci (2009) 0.99

Generation of candidate ligands for nicotinic acetylcholine receptors via in situ click chemistry with a soluble acetylcholine binding protein template. J Am Chem Soc (2012) 0.99

Structural characterization of recombinant soluble rat neuroligin 1: mapping of secondary structure and glycosylation by mass spectrometry. Biochemistry (2004) 0.97

Residues in the epsilon subunit of the nicotinic acetylcholine receptor interact to confer selectivity of waglerin-1 for the alpha-epsilon subunit interface site. Biochemistry (2002) 0.97

Inherited genetic variants in autism-related CNTNAP2 show perturbed trafficking and ATF6 activation. Hum Mol Genet (2012) 0.96

Defining nicotinic agonist binding surfaces through photoaffinity labeling. Biochemistry (2007) 0.96

Direct analysis of the kinetic profiles of organophosphate-acetylcholinesterase adducts by MALDI-TOF mass spectrometry. Biochemistry (2003) 0.96

Nicotinic acetylcholine receptor distribution in relation to spinal neurotransmission pathways. J Comp Neurol (2003) 0.95

Inhibitors of different structure induce distinguishing conformations in the omega loop, Cys69-Cys96, of mouse acetylcholinesterase. J Biol Chem (2002) 0.95

Creating an α7 nicotinic acetylcholine recognition domain from the acetylcholine-binding protein: crystallographic and ligand selectivity analyses. J Biol Chem (2011) 0.95

Mutation of acetylcholinesterase to enhance oxime-assisted catalytic turnover of methylphosphonates. Toxicology (2006) 0.95

Neuroligin trafficking deficiencies arising from mutations in the alpha/beta-hydrolase fold protein family. J Biol Chem (2010) 0.95

Institutional Profile: University of California San Diego Pharmacogenomics Education Program (PharmGenEd™): bridging the gap between science and practice. Pharmacogenomics (2011) 0.94