PubRank

Wim G J Hol

Author PubWeight™ 137.03‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Two sensor kinases contribute to the hypoxic response of Mycobacterium tuberculosis. J Biol Chem 2004 2.71
2 Type II secretion: from structure to function. FEMS Microbiol Lett 2006 2.51
3 Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes. Mol Biochem Parasitol 2006 2.45
4 Toxoplasma gondii calcium-dependent protein kinase 1 is a target for selective kinase inhibitors. Nat Struct Mol Biol 2010 2.34
5 Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system. Nat Struct Mol Biol 2008 2.19
6 The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP binding. Proc Natl Acad Sci U S A 2002 2.10
7 The type II secretion system: biogenesis, molecular architecture and mechanism. Nat Rev Microbiol 2012 1.96
8 Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody. Structure 2009 1.89
9 Sites of interaction between aldolase and thrombospondin-related anonymous protein in plasmodium. Mol Biol Cell 2003 1.83
10 The 1.6-A crystal structure of the class of chaperones represented by Escherichia coli Hsp31 reveals a putative catalytic triad. Proc Natl Acad Sci U S A 2003 1.72
11 High resolution crystal structure of a key editosome enzyme from Trypanosoma brucei: RNA editing ligase 1. J Mol Biol 2004 1.67
12 Structural basis for the activation of cholera toxin by human ARF6-GTP. Science 2005 1.66
13 Structural basis for UTP specificity of RNA editing TUTases from Trypanosoma brucei. EMBO J 2005 1.61
14 Secretins: dynamic channels for protein transport across membranes. Trends Biochem Sci 2011 1.58
15 Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae. J Mol Biol 2003 1.55
16 The crystal structure of human tyrosyl-DNA phosphodiesterase, Tdp1. Structure 2002 1.51
17 An improved protocol for rapid freezing of protein samples for long-term storage. Acta Crystallogr D Biol Crystallogr 2003 1.49
18 Crystal structure of a transition state mimic for Tdp1 assembled from vanadate, DNA, and a topoisomerase I-derived peptide. Chem Biol 2003 1.47
19 Structure of the cholera toxin secretion channel in its closed state. Nat Struct Mol Biol 2010 1.44
20 Insights into substrate binding and catalytic mechanism of human tyrosyl-DNA phosphodiesterase (Tdp1) from vanadate and tungstate-inhibited structures. J Mol Biol 2002 1.37
21 Selective inhibitors of methionyl-tRNA synthetase have potent activity against Trypanosoma brucei Infection in Mice. Antimicrob Agents Chemother 2011 1.37
22 Multiple determinants for selective inhibition of apicomplexan calcium-dependent protein kinase CDPK1. J Med Chem 2012 1.35
23 Urea-based inhibitors of Trypanosoma brucei methionyl-tRNA synthetase: selectivity and in vivo characterization. J Med Chem 2012 1.35
24 The X-ray structure of the type II secretion system complex formed by the N-terminal domain of EpsE and the cytoplasmic domain of EpsL of Vibrio cholerae. J Mol Biol 2005 1.33
25 The power of vanadate in crystallographic investigations of phosphoryl transfer enzymes. FEBS Lett 2004 1.32
26 Aldolase provides an unusual binding site for thrombospondin-related anonymous protein in the invasion machinery of the malaria parasite. Proc Natl Acad Sci U S A 2007 1.31
27 GAF domains: two-billion-year-old molecular switches that bind cyclic nucleotides. Mol Interv 2002 1.29
28 Structures of Mycobacterium tuberculosis DosR and DosR-DNA complex involved in gene activation during adaptation to hypoxic latency. J Mol Biol 2005 1.26
29 Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands. J Biomol Screen 2009 1.25
30 Characterization of Trypanosoma brucei dihydroorotate dehydrogenase as a possible drug target; structural, kinetic and RNAi studies. Mol Microbiol 2008 1.22
31 Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids. EMBO J 2006 1.21
32 Crystal structures of the response regulator DosR from Mycobacterium tuberculosis suggest a helix rearrangement mechanism for phosphorylation activation. J Mol Biol 2008 1.20
33 Structural genomics of pathogenic protozoa: an overview. Methods Mol Biol 2008 1.20
34 Distinct states of methionyl-tRNA synthetase indicate inhibitor binding by conformational selection. Structure 2012 1.18
35 The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery. J Mol Biol 2007 1.15
36 The structure of the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae: an unusual member of the actin-like ATPase superfamily. J Mol Biol 2004 1.14
37 The structure of the transition state of the heterodimeric topoisomerase I of Leishmania donovani as a vanadate complex with nicked DNA. J Mol Biol 2006 1.14
38 Structural and functional studies of EpsC, a crucial component of the type 2 secretion system from Vibrio cholerae. J Mol Biol 2006 1.13
39 Structure of the Mycobacterium tuberculosis flavin dependent thymidylate synthase (MtbThyX) at 2.0A resolution. J Mol Biol 2005 1.13
40 Structural and functional studies on the interaction of GspC and GspD in the type II secretion system. PLoS Pathog 2011 1.12
41 Structure of the minor pseudopilin EpsH from the Type 2 secretion system of Vibrio cholerae. J Mol Biol 2007 1.12
42 The role of medical structural genomics in discovering new drugs for infectious diseases. PLoS Comput Biol 2009 1.11
43 Crystal structures of an intrinsically active cholera toxin mutant yield insight into the toxin activation mechanism. Biochemistry 2004 1.09
44 Structure of the MTIP-MyoA complex, a key component of the malaria parasite invasion motor. Proc Natl Acad Sci U S A 2006 1.09
45 Parasitology. Drugs to combat tropical protozoan parasites. Science 2002 1.08
46 A protein-protein interaction map of trypanosome ~20S editosomes. J Biol Chem 2009 1.08
47 Solution and crystallographic studies of branched multivalent ligands that inhibit the receptor-binding of cholera toxin. J Am Chem Soc 2002 1.08
48 Explorations of peptide and oligonucleotide binding sites of tyrosyl-DNA phosphodiesterase using vanadate complexes. J Med Chem 2004 1.07
49 The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the type 2 secretion system of Vibrio vulnificus. J Mol Biol 2007 1.07
50 In vivo cross-linking of EpsG to EpsL suggests a role for EpsL as an ATPase-pseudopilin coupling protein in the Type II secretion system of Vibrio cholerae. Mol Microbiol 2011 1.07
51 Calcium is essential for the major pseudopilin in the type 2 secretion system. J Biol Chem 2009 1.05
52 Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation. Biochemistry 2004 1.05
53 The crystal structure of the periplasmic domain of the type II secretion system protein EpsM from Vibrio cholerae: the simplest version of the ferredoxin fold. J Mol Biol 2004 1.04
54 Use of thermal melt curves to assess the quality of enzyme preparations. Anal Biochem 2009 1.02
55 Biogenesis of peroxisomes and glycosomes: trypanosomatid glycosome assembly is a promising new drug target. FEMS Microbiol Rev 2004 1.02
56 Crystal structure of an IdeR-DNA complex reveals a conformational change in activated IdeR for base-specific interactions. J Mol Biol 2004 1.01
57 Functional studies of the Mycobacterium tuberculosis iron-dependent regulator. J Biol Chem 2004 1.01
58 Characterization and crystal structure of a high-affinity pentavalent receptor-binding inhibitor for cholera toxin and E. coli heat-labile enterotoxin. J Am Chem Soc 2002 1.00
59 Using fragment cocktail crystallography to assist inhibitor design of Trypanosoma brucei nucleoside 2-deoxyribosyltransferase. J Med Chem 2006 1.00
60 Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function. Acta Crystallogr Sect F Struct Biol Cryst Commun 2008 0.99
61 Structures of substrate- and inhibitor-bound adenosine deaminase from a human malaria parasite show a dramatic conformational change and shed light on drug selectivity. J Mol Biol 2008 0.98
62 Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody. Nucleic Acids Res 2011 0.97
63 Structural insights into the recognition of peroxisomal targeting signal 1 by Trypanosoma brucei peroxin 5. J Mol Biol 2008 0.97
64 Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs. J Mol Biol 2010 0.97
65 Precursor structure of cephalosporin acylase. Insights into autoproteolytic activation in a new N-terminal hydrolase family. J Biol Chem 2001 0.96
66 The binding of cholera toxin to the periplasmic vestibule of the type II secretion channel. Channels (Austin) 2011 0.96
67 A new native EcHsp31 structure suggests a key role of structural flexibility for chaperone function. Protein Sci 2004 0.96
68 Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies. J Struct Biol 2010 0.95
69 The role of lysine 532 in the catalytic mechanism of human topoisomerase I. J Biol Chem 2003 0.95
70 Crystal structures, metal activation, and DNA-binding properties of two-domain IdeR from Mycobacterium tuberculosis. Biochemistry 2007 0.95
71 Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer. Chem Biol 2004 0.95
72 The dimer formed by the periplasmic domain of EpsL from the Type 2 Secretion System of Vibrio parahaemolyticus. J Struct Biol 2009 0.94
73 Structure of Leishmania major methionyl-tRNA synthetase in complex with intermediate products methionyladenylate and pyrophosphate. Biochimie 2010 0.93
74 Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major. Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 0.93
75 Oligomerization of EpsE coordinates residues from multiple subunits to facilitate ATPase activity. J Biol Chem 2011 0.93
76 The linker-loop region of Escherichia coli chaperone Hsp31 functions as a gate that modulates high-affinity substrate binding at elevated temperatures. Proc Natl Acad Sci U S A 2004 0.92
77 Crystals of peptide deformylase from Plasmodium falciparum reveal critical characteristics of the active site for drug design. Structure 2002 0.92
78 Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins. Eur J Biochem 2003 0.92
79 Crystal structure of GAP50, the anchor of the invasion machinery in the inner membrane complex of Plasmodium falciparum. J Struct Biol 2012 0.92
80 The three-dimensional structure of the cytoplasmic domains of EpsF from the type 2 secretion system of Vibrio cholerae. J Struct Biol 2009 0.91
81 Structure determination of glycogen synthase kinase-3 from Leishmania major and comparative inhibitor structure-activity relationships with Trypanosoma brucei GSK-3. Mol Biochem Parasitol 2010 0.90
82 3,5-Substituted phenyl galactosides as leads in designing effective cholera toxin antagonists; synthesis and crystallographic studies. Bioorg Med Chem 2004 0.90
83 Fragment-based cocktail crystallography by the medical structural genomics of pathogenic protozoa consortium. Curr Top Med Chem 2009 0.90
84 Hexamers of the type II secretion ATPase GspE from Vibrio cholerae with increased ATPase activity. Structure 2013 0.89
85 NADP+ expels both the co-factor and a substrate analog from the Mycobacterium tuberculosis ThyX active site: opportunities for anti-bacterial drug design. J Mol Biol 2006 0.89
86 Leishmania mexicana glycerol-3-phosphate dehydrogenase showed conformational changes upon binding a bi-substrate adduct. J Mol Biol 2003 0.88
87 The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase. J Mol Biol 2010 0.88
88 Cholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system. Infect Immun 2003 0.87
89 Protein heterodimerization through ligand-bridged multivalent pre-organization: enhancing ligand binding toward both protein targets. J Am Chem Soc 2005 0.87
90 Structure-based design of a macrocyclic inhibitor for peptide deformylase. J Med Chem 2003 0.86
91 Crystal structures and proposed structural/functional classification of three protozoan proteins from the isochorismatase superfamily. Protein Sci 2005 0.85
92 The double-length tyrosyl-tRNA synthetase from the eukaryote Leishmania major forms an intrinsically asymmetric pseudo-dimer. J Mol Biol 2011 0.85
93 Structures of Plasmodium falciparum purine nucleoside phosphorylase complexed with sulfate and its natural substrate inosine. Acta Crystallogr D Biol Crystallogr 2005 0.85
94 Analysis of the sequence motifs responsible for the interactions of peroxins 14 and 5, which are involved in glycosome biogenesis in Trypanosoma brucei. Biochemistry 2003 0.84
95 Induced resistance to methionyl-tRNA synthetase inhibitors in Trypanosoma brucei is due to overexpression of the target. Antimicrob Agents Chemother 2013 0.84
96 Crystal structures of three protozoan homologs of tryptophanyl-tRNA synthetase. Mol Biochem Parasitol 2011 0.84
97 Structure of the conserved hypothetical protein MAL13P1.257 from Plasmodium falciparum. Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 0.84
98 Large cyclic peptides as cores of multivalent ligands: application to inhibitors of receptor binding by cholera toxin. J Org Chem 2004 0.84
99 Structure of ribose 5-phosphate isomerase from Plasmodium falciparum. Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 0.83
100 Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum at 2.25 A resolution reveals intriguing extra electron density in the active site. Proteins 2006 0.83
101 The structure of the C-terminal domain of the largest editosome interaction protein and its role in promoting RNA binding by RNA-editing ligase L2. Nucleic Acids Res 2012 0.82
102 Anchor-based design of improved cholera toxin and E. coli heat-labile enterotoxin receptor binding antagonists that display multiple binding modes. Chem Biol 2002 0.82
103 Prediction of protein crystallization outcome using a hybrid method. J Struct Biol 2010 0.82
104 An improved crystal form of Plasmodium falciparum peptide deformylase. Protein Sci 2004 0.82
105 Modeling the interaction between aldolase and the thrombospondin-related anonymous protein, a key connection of the malaria parasite invasion machinery. Proteins 2007 0.81
106 Crystal structures of Plasmodium falciparum cytosolic tryptophanyl-tRNA synthetase and its potential as a target for structure-guided drug design. Mol Biochem Parasitol 2013 0.81
107 The structure of tryptophanyl-tRNA synthetase from Giardia lamblia reveals divergence from eukaryotic homologs. J Struct Biol 2010 0.80
108 Functional and structural characterization of Vibrio cholerae extracellular serine protease B, VesB. J Biol Chem 2014 0.80
109 Crystal structure of the pilotin from the enterohemorrhagic Escherichia coli type II secretion system. J Struct Biol 2013 0.80
110 Structure of the prolyl-tRNA synthetase from the eukaryotic pathogen Giardia lamblia. Acta Crystallogr D Biol Crystallogr 2012 0.79
111 A dodecameric ring-like structure of the N0 domain of the type II secretin from enterotoxigenic Escherichia coli. J Struct Biol 2013 0.79
112 Crystal structure of the aspartyl-tRNA synthetase from Entamoeba histolytica. Mol Biochem Parasitol 2009 0.78
113 Explorations of linked editosome domains leading to the discovery of motifs defining conserved pockets in editosome OB-folds. J Struct Biol 2012 0.78
114 Structural biology and structure-based inhibitor design of cholera toxin and heat-labile enterotoxin. Int J Med Microbiol 2004 0.78
115 Structure of a low-melting-temperature anti-cholera toxin: llama V(H)H domain. Acta Crystallogr Sect F Struct Biol Cryst Commun 2013 0.77
116 Solution- and solid-phase syntheses of guanidine-bridged, water-soluble linkers for multivalent ligand design. Org Lett 2004 0.77
117 Anomalous differences of light elements in determining precise binding modes of ligands to glycerol-3-phosphate dehydrogenase. Chem Biol 2002 0.77
118 The compact conformation of the Plasmodium knowlesi myosin tail interacting protein MTIP in complex with the C-terminal helix of myosin A. Mol Biochem Parasitol 2013 0.77
119 Structure of a ribulose 5-phosphate 3-epimerase from Plasmodium falciparum. Proteins 2006 0.77
120 The structure of the D3 domain of Plasmodium falciparum myosin tail interacting protein MTIP in complex with a nanobody. Mol Biochem Parasitol 2013 0.77
121 The structure of Plasmodium vivax phosphatidylethanolamine-binding protein suggests a functional motif containing a left-handed helix. Acta Crystallogr Sect F Struct Biol Cryst Commun 2007 0.76
122 Screening a fragment cocktail library using ultrafiltration. Anal Bioanal Chem 2011 0.75
123 From cells, to mice, to target: Characterization of NEU-1053 (SB-443342) and its analogs for treatment of human African trypanosomiasis. ACS Infect Dis 2017 0.75
124 Drug design for the third world: a conference report. Proteins 2007 0.75