Published in Protein Sci on September 28, 2007
PoPMuSiC 2.1: a web server for the estimation of protein stability changes upon mutation and sequence optimality. BMC Bioinformatics (2011) 1.76
An overview of enzymatic reagents for the removal of affinity tags. Protein Expr Purif (2011) 1.46
A novel method for high-level production of TEV protease by superfolder GFP tag. J Biomed Biotechnol (2010) 1.15
The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal. Protein Expr Purif (2011) 0.96
Structural and functional analysis of the pore-forming toxin NetB from Clostridium perfringens. MBio (2013) 0.95
Rapid modification of proteins using a rapamycin-inducible tobacco etch virus protease system. PLoS One (2009) 0.93
Flanking domain stability modulates the aggregation kinetics of a polyglutamine disease protein. Protein Sci (2011) 0.93
Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization. Sci Rep (2016) 0.90
Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase. Nucleic Acids Res (2013) 0.83
PqsBC, a Condensing Enzyme in the Biosynthesis of the Pseudomonas aeruginosa Quinolone Signal: CRYSTAL STRUCTURE, INHIBITION, AND REACTION MECHANISM. J Biol Chem (2016) 0.81
Structure-function relation of phospholamban: modulation of channel activity as a potential regulator of SERCA activity. PLoS One (2013) 0.80
Cloning, expression, purification, crystallization and preliminary X-ray analysis of EaLsc, a levansucrase from Erwinia amylovora. Acta Crystallogr Sect F Struct Biol Cryst Commun (2013) 0.80
Molecular insights into the interaction between Plasmodium falciparum apical membrane antigen 1 and an invasion-inhibitory peptide. PLoS One (2014) 0.79
Enhanced thermostability of keratinase by computational design and empirical mutation. J Ind Microbiol Biotechnol (2013) 0.79
His224 alters the R2 drug binding site and Phe218 influences the catalytic efficiency of the metallo-β-lactamase VIM-7. Antimicrob Agents Chemother (2014) 0.78
Expression, purification, crystallization and preliminary X-ray analysis of glucose-1-phosphate uridylyltransferase (GalU) from Erwinia amylovora. Acta Crystallogr F Struct Biol Commun (2014) 0.76
Axin cancer mutants form nanoaggregates to rewire the Wnt signaling network. Nat Struct Mol Biol (2016) 0.75
A new fusion protein platform for quantitatively measuring activity of multiple proteases. Microb Cell Fact (2014) 0.75
Design strategies to address the effect of hydrophobic epitope on stability and in vitro assembly of modular virus-like particle. Protein Sci (2016) 0.75
Fragment library screening identifies hits that bind to the non-catalytic surface of Pseudomonas aeruginosa DsbA1. PLoS One (2017) 0.75
Insight into the structural stability of wild type and mutants of the tobacco etch virus protease with molecular dynamics simulations. J Mol Model (2013) 0.75
Computational tools help improve protein stability but with a solubility tradeoff. J Biol Chem (2017) 0.75
The Protein Data Bank. Nucleic Acids Res (2000) 187.10
Selection of representative protein data sets. Protein Sci (1992) 15.62
Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol (1984) 13.69
Experimentally determined hydrophobicity scale for proteins at membrane interfaces. Nat Struct Biol (1996) 8.57
Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng (2001) 5.71
Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci (1999) 5.43
The P1' specificity of tobacco etch virus protease. Biochem Biophys Res Commun (2002) 2.79
Predicting protein stability changes upon mutation using database-derived potentials: solvent accessibility determines the importance of local versus non-local interactions along the sequence. J Mol Biol (1997) 2.38
Improved solubility of TEV protease by directed evolution. J Biotechnol (2005) 2.35
Factors influencing the ability of knowledge-based potentials to identify native sequence-structure matches. J Mol Biol (1994) 1.86
Structural basis for the substrate specificity of tobacco etch virus protease. J Biol Chem (2002) 1.72
Stability changes upon mutation of solvent-accessible residues in proteins evaluated by database-derived potentials. J Mol Biol (1996) 1.50
A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnol (2006) 1.48
PoPMuSiC, an algorithm for predicting protein mutant stability changes: application to prion proteins. Protein Eng (2000) 1.48
Directed evolution of proteins for heterologous expression and stability. Curr Opin Struct Biol (2005) 1.43
Automatic classification and analysis of alpha alpha-turn motifs in proteins. J Mol Biol (1996) 1.41
Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form. Virology (1995) 1.39
Protein aggregation during overexpression limited by peptide extensions with large net negative charge. Protein Expr Purif (2004) 1.34
Protein expression and refolding--a practical guide to getting the most out of inclusion bodies. Biotechnol Annu Rev (2004) 1.08
Probing the equilibrium denaturation of the serpin alpha(1)-antitrypsin with single tryptophan mutants; evidence for structure in the urea unfolded state. J Mol Biol (2001) 1.07
PoPMuSiC, rationally designing point mutations in protein structures. Bioinformatics (2002) 1.06
Investigating the effects of mutations on protein aggregation in the cell. J Biol Chem (2004) 1.01
REFOLD: an analytical database of protein refolding methods. Protein Expr Purif (2005) 0.99
Effect of salts on the stability and folding of staphylococcal nuclease. Biochemistry (2001) 0.91
In vitro and in silico design of alpha1-antitrypsin mutants with different conformational stabilities. J Mol Biol (2003) 0.86
A common fold mediates vertebrate defense and bacterial attack. Science (2007) 3.43
Fast and accurate predictions of protein stability changes upon mutations using statistical potentials and neural networks: PoPMuSiC-2.0. Bioinformatics (2009) 2.03
PoPMuSiC 2.1: a web server for the estimation of protein stability changes upon mutation and sequence optimality. BMC Bioinformatics (2011) 1.76
Functional insights from the distribution and role of homopeptide repeat-containing proteins. Genome Res (2005) 1.70
The two-stage pathway of ataxin-3 fibrillogenesis involves a polyglutamine-independent step. J Biol Chem (2006) 1.66
The 1.5 A crystal structure of a prokaryote serpin: controlling conformational change in a heated environment. Structure (2003) 1.61
Dopamine promotes alpha-synuclein aggregation into SDS-resistant soluble oligomers via a distinct folding pathway. FASEB J (2005) 1.51
A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnol (2006) 1.48
Molecular gymnastics: serpin structure, folding and misfolding. Curr Opin Struct Biol (2006) 1.47
Natural HLA class I polymorphism controls the pathway of antigen presentation and susceptibility to viral evasion. J Exp Med (2004) 1.43
Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor. Immunity (2009) 1.40
MyD88 adapter-like (Mal)/TIRAP interaction with TRAF6 is critical for TLR2- and TLR4-mediated NF-kappaB proinflammatory responses. J Biol Chem (2009) 1.34
Serpins flex their muscle: I. Putting the clamps on proteolysis in diverse biological systems. J Biol Chem (2010) 1.32
Mechanisms of ataxin-3 misfolding and fibril formation: kinetic analysis of a disease-associated polyglutamine protein. J Mol Biol (2007) 1.29
The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix. J Biol Chem (2005) 1.21
BeAtMuSiC: Prediction of changes in protein-protein binding affinity on mutations. Nucleic Acids Res (2013) 1.21
Community-wide evaluation of methods for predicting the effect of mutations on protein-protein interactions. Proteins (2013) 1.20
The murine orthologue of human antichymotrypsin: a structural paradigm for clade A3 serpins. J Biol Chem (2005) 1.20
Formation of a high affinity lipid-binding intermediate during the early aggregation phase of alpha-synuclein. Biochemistry (2008) 1.18
Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation. J Biol Chem (2002) 1.17
Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3. Biochem Biophys Res Commun (2004) 1.14
Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions. J Biol Chem (2010) 1.14
Multi-domain misfolding: understanding the aggregation pathway of polyglutamine proteins. Protein Eng Des Sel (2009) 1.12
Small heat-shock proteins interact with a flanking domain to suppress polyglutamine aggregation. Proc Natl Acad Sci U S A (2010) 1.11
The subtilisin-like protease AprV2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates. PLoS Pathog (2010) 1.10
Kinetic instability of the serpin Z alpha1-antitrypsin promotes aggregation. J Mol Biol (2009) 1.10
Specificity and phenetic relationships of iron- and manganese-containing superoxide dismutases on the basis of structure and sequence comparisons. J Biol Chem (2003) 1.09
Protein expression and refolding--a practical guide to getting the most out of inclusion bodies. Biotechnol Annu Rev (2004) 1.08
Polyglutamine expansion in ataxin-3 does not affect protein stability: implications for misfolding and disease. J Biol Chem (2004) 1.07
Acid Denaturation of alpha1-antitrypsin: characterization of a novel mechanism of serpin polymerization. J Mol Biol (2002) 1.06
Different conformational changes within the F-helix occur during serpin folding, polymerization, and proteinase inhibition. Biochemistry (2004) 1.06
Antithrombin: in control of coagulation. Int J Biochem Cell Biol (2004) 1.03
The matrix refolded. Nat Methods (2005) 1.01
Human embryonic stem cell models of Huntington disease. Reprod Biomed Online (2009) 0.99
REFOLD: an analytical database of protein refolding methods. Protein Expr Purif (2005) 0.99
Evidence that interaction between conserved residues in transmembrane helices 2, 3, and 7 are crucial for human VPAC1 receptor activation. Mol Pharmacol (2010) 0.99
Destabilization of a non-pathological variant of ataxin-3 results in fibrillogenesis via a partially folded intermediate: a model for misfolding in polyglutamine disease. J Mol Biol (2004) 0.98
The first peptides: the evolutionary transition between prebiotic amino acids and early proteins. J Theor Biol (2009) 0.98
Stabilization of neurotoxic soluble beta-sheet-rich conformations of the Alzheimer's disease amyloid-beta peptide. Biophys J (2007) 0.98
The N terminus of the serpin, tengpin, functions to trap the metastable native state. EMBO Rep (2007) 0.97
A conserved cysteine residue is involved in disulfide bond formation between plant plasma membrane aquaporin monomers. Biochem J (2012) 0.96
Sequence-structure signals of 3D domain swapping in proteins. J Mol Biol (2003) 0.96
Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteases. Biochemistry (2002) 0.96
Purification of polyglutamine proteins. Methods Enzymol (2006) 0.94
The structure of the bacterial oxidoreductase enzyme DsbA in complex with a peptide reveals a basis for substrate specificity in the catalytic cycle of DsbA enzymes. J Biol Chem (2009) 0.94
Probing the role of the F-helix in serpin stability through a single tryptophan substitution. Biochemistry (2002) 0.94
X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation. EMBO J (2006) 0.94
Probing the energetic and structural role of amino acid/nucleobase cation-pi interactions in protein-ligand complexes. J Biol Chem (2002) 0.94
Physical characterization of serpin conformations. Methods (2004) 0.93
A protein family under 'stress' - serpin stability, folding and misfolding. Front Biosci (2005) 0.93
Crystal structure of a UDP-glucose-specific glycosyltransferase from a Mycobacterium species. J Biol Chem (2008) 0.93
Flanking domain stability modulates the aggregation kinetics of a polyglutamine disease protein. Protein Sci (2011) 0.93
The structural impact of a polyglutamine tract is location-dependent. Biophys J (2008) 0.93
DNA accelerates the inhibition of human cathepsin V by serpins. J Biol Chem (2007) 0.92
Production of recombinant serpins in Escherichia coli. Methods (2004) 0.92
Expression, purification and characterization of recombinant Z alpha(1)-antitrypsin--the most common cause of alpha(1)-antitrypsin deficiency. Protein Expr Purif (2009) 0.91
PolyQ: a database describing the sequence and domain context of polyglutamine repeats in proteins. Nucleic Acids Res (2010) 0.91
Serpin polymerization and its role in disease--the molecular basis of alpha1-antitrypsin deficiency. IUBMB Life (2009) 0.91
PFD: a database for the investigation of protein folding kinetics and stability. Nucleic Acids Res (2005) 0.90
Structural change in β-sheet A of Z α(1)-antitrypsin is responsible for accelerated polymerization and disease. J Mol Biol (2011) 0.90
Promiscuous beta-strand interactions and the conformational diseases. Curr Med Chem (2004) 0.90
The structure of H-2K(b) and K(bm8) complexed to a herpes simplex virus determinant: evidence for a conformational switch that governs T cell repertoire selection and viral resistance. J Immunol (2004) 0.90
Structure-based mutant stability predictions on proteins of unknown structure. J Biotechnol (2012) 0.90
Stair motifs at protein-DNA interfaces: nonadditivity of H-bond, stacking, and cation-pi interactions. J Am Chem Soc (2004) 0.89
Protein stability: a single recorded mutation aids in predicting the effects of other mutations in the same amino acid site. Bioinformatics (2011) 0.89
The REFOLD database: a tool for the optimization of protein expression and refolding. Nucleic Acids Res (2006) 0.89
Thermostability of salt bridges versus hydrophobic interactions in proteins probed by statistical potentials. J Chem Inf Model (2007) 0.88
The high resolution crystal structure of a native thermostable serpin reveals the complex mechanism underpinning the stressed to relaxed transition. J Biol Chem (2004) 0.88
Development of novel statistical potentials describing cation-pi interactions in proteins and comparison with semiempirical and quantum chemistry approaches. J Chem Inf Model (2006) 0.88
A structural basis for loop C-sheet polymerization in serpins. J Mol Biol (2008) 0.87
In vitro and in silico design of alpha1-antitrypsin mutants with different conformational stabilities. J Mol Biol (2003) 0.86
Antagonism of antiviral and allogeneic activity of a human public CTL clonotype by a single altered peptide ligand: implications for allograft rejection. J Immunol (2005) 0.86
Aeropin from the extremophile Pyrobaculum aerophilum bypasses the serpin misfolding trap. J Biol Chem (2007) 0.86
How do proteins avoid becoming too stable? Biophysical studies into metastable proteins. Eur Biophys J (2003) 0.86
Free-energy calculations of protein-ligand cation-pi and amino-pi interactions: from vacuum to proteinlike environments. J Am Chem Soc (2003) 0.86
SODa: an Mn/Fe superoxide dismutase prediction and design server. BMC Bioinformatics (2008) 0.86
Thermo- and mesostabilizing protein interactions identified by temperature-dependent statistical potentials. Biophys J (2010) 0.85
Conformational properties of the disease-causing Z variant of α1-antitrypsin revealed by theory and experiment. Biophys J (2012) 0.85
Structural and biochemical characterization of the oxidoreductase NmDsbA3 from Neisseria meningitidis. J Biol Chem (2008) 0.85