Activity-based probes as a tool for functional proteomic analysis of proteases.

PubWeight™: 1.26‹?› | Rank: Top 10%

🔗 View Article (PMC 2997944)

Published in Expert Rev Proteomics on October 01, 2008

Authors

Marko Fonović1, Matthew Bogyo

Author Affiliations

1: Department of Biochemistry, Molecular and Structural Biology, JoZef Stefan Institute, Jamova Cesta 39, SI-1000 Ljubljana, Slovenia. marko.fonovic@ijs.si

Articles citing this

Haem-activated promiscuous targeting of artemisinin in Plasmodium falciparum. Nat Commun (2015) 1.90

Activity-based metabolomic profiling of enzymatic function: identification of Rv1248c as a mycobacterial 2-hydroxy-3-oxoadipate synthase. Chem Biol (2010) 1.60

Hemoglobin digestion in blood-feeding ticks: mapping a multipeptidase pathway by functional proteomics. Chem Biol (2009) 1.17

Caspase substrates and inhibitors. Cold Spring Harb Perspect Biol (2013) 1.07

Cathepsin S is activated during colitis and causes visceral hyperalgesia by a PAR2-dependent mechanism in mice. Gastroenterology (2011) 1.03

Imaging enzymes at work: metabolic mapping by enzyme histochemistry. J Histochem Cytochem (2010) 0.97

Cathepsin B trafficking in thyroid carcinoma cells. Thyroid Res (2011) 0.93

A quantitative chemical proteomics approach to profile the specific cellular targets of andrographolide, a promising anticancer agent that suppresses tumor metastasis. Mol Cell Proteomics (2014) 0.92

An activity-based imaging probe for the integral membrane hydrolase KIAA1363. Angew Chem Int Ed Engl (2011) 0.90

Nuclear cysteine cathepsin variants in thyroid carcinoma cells. Biol Chem (2010) 0.88

The cAMP capture compound mass spectrometry as a novel tool for targeting cAMP-binding proteins: from protein kinase A to potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channels. Mol Cell Proteomics (2009) 0.87

Proteomics of protein post-translational modifications implicated in neurodegeneration. Transl Neurodegener (2014) 0.86

Mapping sites of aspirin-induced acetylations in live cells by quantitative acid-cleavable activity-based protein profiling (QA-ABPP). Sci Rep (2015) 0.85

Ferrous iron-dependent delivery of therapeutic agents to the malaria parasite. Future Med Chem (2012) 0.83

The antimalarial natural product symplostatin 4 is a nanomolar inhibitor of the food vacuole falcipains. Chem Biol (2012) 0.83

Chemical proteomics approaches for identifying the cellular targets of natural products. Nat Prod Rep (2016) 0.83

Cysteine cathepsins: their role in tumor progression and recent trends in the development of imaging probes. Front Chem (2015) 0.80

Target discovery of acivicin in cancer cells elucidates its mechanism of growth inhibition†Electronic supplementary information (ESI) available: Synthesis, cloning, protein expression, purification and biochemical assays. See DOI: 10.1039/c4sc02339k. Chem Sci (2014) 0.79

Selective inhibition of plant serine hydrolases by agrochemicals revealed by competitive ABPP. Bioorg Med Chem (2011) 0.79

Finding enzymes that are actively involved in cancer. Proc Natl Acad Sci U S A (2010) 0.78

Integrative proteomic profiling of protein activity and interactions using protein arrays. Mol Cell Proteomics (2012) 0.78

A sequence and structure based method to predict putative substrates, functions and regulatory networks of endo proteases. PLoS One (2009) 0.77

Extracellular cathepsin S and intracellular caspase 1 activation are surrogate biomarkers of particulate-induced lysosomal disruption in macrophages. Part Fibre Toxicol (2016) 0.76

Small-molecule probes elucidate global enzyme activity in a proteomic context. BMB Rep (2014) 0.75

Evaluation of sulfatase-directed quinone methide traps for proteomics. Bioorg Med Chem (2011) 0.75

Ubiquitylation as a Rheostat for TCR Signaling: From Targeted Approaches Toward Global Profiling. Front Immunol (2015) 0.75

Affinity-based profiling of dehydrogenase subproteomes. Methods Mol Biol (2012) 0.75

Integrating Pharmacoproteomics into Early-Phase Clinical Development: State-of-the-Art, Challenges, and Recommendations. Int J Mol Sci (2017) 0.75

Pathomimetic cancer avatars for live-cell imaging of protease activity. Biochimie (2015) 0.75

Towards the development of activity-based probes for detection of lysine-specific demethylase-1 activity. Bioorg Med Chem (2016) 0.75

Articles cited by this

A stepwise huisgen cycloaddition process: copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes. Angew Chem Int Ed Engl (2002) 24.12

Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science (1995) 8.58

Profiling enzyme activities in vivo using click chemistry methods. Chem Biol (2004) 5.97

Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast. Cell (2000) 5.69

Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity. Proc Natl Acad Sci U S A (1999) 5.28

Activity-based protein profiling in vivo using a copper(i)-catalyzed azide-alkyne [3 + 2] cycloaddition. J Am Chem Soc (2003) 4.92

Irreversible inhibitors of serine, cysteine, and threonine proteases. Chem Rev (2002) 4.57

Cathepsin cysteine proteases are effectors of invasive growth and angiogenesis during multistage tumorigenesis. Cancer Cell (2004) 4.38

MEROPS: the peptidase database. Nucleic Acids Res (2006) 4.20

Photoactivated gamma-secretase inhibitors directed to the active site covalently label presenilin 1. Nature (2000) 4.12

Editing of ubiquitin conjugates by an isopeptidase in the 26S proteasome. Nature (1997) 3.47

Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family. Chem Biol (2002) 3.43

A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14. EMBO J (2001) 3.42

Discovering potent and selective reversible inhibitors of enzymes in complex proteomes. Nat Biotechnol (2003) 3.17

Direct visualization of serine hydrolase activities in complex proteomes using fluorescent active site-directed probes. Proteomics (2001) 3.03

A streamlined platform for high-content functional proteomics of primary human specimens. Nat Methods (2005) 3.00

The active sites of the eukaryotic 20 S proteasome and their involvement in subunit precursor processing. J Biol Chem (1997) 2.85

Epoxide electrophiles as activity-dependent cysteine protease profiling and discovery tools. Chem Biol (2000) 2.84

Covalent modification of the active site threonine of proteasomal beta subunits and the Escherichia coli homolog HslV by a new class of inhibitors. Proc Natl Acad Sci U S A (1997) 2.81

Noninvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes. Nat Chem Biol (2007) 2.80

Protein ligation: an enabling technology for the biophysical analysis of proteins. Nat Methods (2006) 2.60

Enzyme activity profiles of the secreted and membrane proteome that depict cancer cell invasiveness. Proc Natl Acad Sci U S A (2002) 2.52

Ubiquitin-aldehyde: a general inhibitor of ubiquitin-recycling processes. Proc Natl Acad Sci U S A (1987) 2.52

Profiling serine hydrolase activities in complex proteomes. Biochemistry (2001) 2.46

Structure-guided development of affinity probes for tyrosine kinases using chemical genetics. Nat Chem Biol (2007) 2.43

A deubiquitinating enzyme encoded by HSV-1 belongs to a family of cysteine proteases that is conserved across the family Herpesviridae. Mol Cell (2005) 2.43

Proteomic profiling of metalloprotease activities with cocktails of active-site probes. Nat Chem Biol (2006) 2.25

The neprilysin (NEP) family of zinc metalloendopeptidases: genomics and function. Bioessays (2001) 2.23

Activity-based probes that target diverse cysteine protease families. Nat Chem Biol (2005) 2.21

Tumor cell-derived and macrophage-derived cathepsin B promotes progression and lung metastasis of mammary cancer. Cancer Res (2006) 2.20

A role for the protease falcipain 1 in host cell invasion by the human malaria parasite. Science (2002) 2.20

A tandem orthogonal proteolysis strategy for high-content chemical proteomics. J Am Chem Soc (2005) 2.14

Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes. Biochemistry (1998) 2.10

Activity-based probes for proteomic profiling of histone deacetylase complexes. Proc Natl Acad Sci U S A (2007) 2.03

Activity-based probes for the proteomic profiling of metalloproteases. Proc Natl Acad Sci U S A (2004) 1.98

Proteomics discovery of metalloproteinase substrates in the cellular context by iTRAQ labeling reveals a diverse MMP-2 substrate degradome. Mol Cell Proteomics (2007) 1.98

Carcinoma and stromal enzyme activity profiles associated with breast tumor growth in vivo. Proc Natl Acad Sci U S A (2004) 1.96

Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)--a general method for mapping sites of probe modification in proteomes. Nat Protoc (2007) 1.87

Cathepsin L in secretory vesicles functions as a prohormone-processing enzyme for production of the enkephalin peptide neurotransmitter. Proc Natl Acad Sci U S A (2003) 1.83

Zinc enzymes. Curr Opin Chem Biol (1998) 1.81

Activity probe for in vivo profiling of the specificity of proteasome inhibitor bortezomib. Nat Methods (2005) 1.78

Chemical approaches for functionally probing the proteome. Mol Cell Proteomics (2002) 1.78

Profiling constitutive proteolytic events in vivo. Biochem J (2007) 1.78

Large scale protein profiling by combination of protein fractionation and multidimensional protein identification technology (MudPIT). Mol Cell Proteomics (2005) 1.71

Selective targeting of lysosomal cysteine proteases with radiolabeled electrophilic substrate analogs. Chem Biol (2000) 1.62

Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. Proc Natl Acad Sci U S A (2004) 1.59

Inactivation of interleukin-1 beta converting enzyme by peptide (acyloxy)methyl ketones. Biochemistry (1994) 1.57

A fluorescent broad-spectrum proteasome inhibitor for labeling proteasomes in vitro and in vivo. Chem Biol (2006) 1.54

High-molecular-weight protein (pUL48) of human cytomegalovirus is a competent deubiquitinating protease: mutant viruses altered in its active-site cysteine or histidine are viable. J Virol (2006) 1.52

Extended peptide-based inhibitors efficiently target the proteasome and reveal overlapping specificities of the catalytic beta-subunits. Chem Biol (2001) 1.52

Biosynthetic processing of cathepsins and lysosomal degradation are abolished in asparaginyl endopeptidase-deficient mice. J Biol Chem (2003) 1.51

Substrate binding and sequence preference of the proteasome revealed by active-site-directed affinity probes. Chem Biol (1998) 1.51

Identification of early intermediates of caspase activation using selective inhibitors and activity-based probes. Mol Cell (2006) 1.43

Mechanism of ubiquitin carboxyl-terminal hydrolase. Borohydride and hydroxylamine inactivate in the presence of ubiquitin. J Biol Chem (1986) 1.43

Small molecule affinity fingerprinting. A tool for enzyme family subclassification, target identification, and inhibitor design. Chem Biol (2002) 1.42

Activity profiling of papain-like cysteine proteases in plants. Plant Physiol (2004) 1.36

A mild chemically cleavable linker system for functional proteomic applications. Angew Chem Int Ed Engl (2007) 1.33

A chemically cleavable biotinylated nucleotide: usefulness in the recovery of protein-DNA complexes from avidin affinity columns. Proc Natl Acad Sci U S A (1985) 1.33

Identification of proteolytic cleavage sites by quantitative proteomics. J Proteome Res (2007) 1.32

Chlamydia trachomatis-derived deubiquitinating enzymes in mammalian cells during infection. Mol Microbiol (2006) 1.30

A genomic analysis of rat proteases and protease inhibitors. Genome Res (2004) 1.27

Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy. Angew Chem Int Ed Engl (2003) 1.25

Defining a link between gap junction communication, proteolysis, and cataract formation. J Biol Chem (2001) 1.13

Identification by functional proteomics of a deubiquitinating/deNeddylating enzyme in Plasmodium falciparum. Mol Microbiol (2006) 1.11

Proteomics evaluation of chemically cleavable activity-based probes. Mol Cell Proteomics (2007) 1.08

Asparagine endopeptidase can initiate the removal of the MHC class II invariant chain chaperone. Immunity (2003) 1.07

Design of cell-permeable, fluorescent activity-based probes for the lysosomal cysteine protease asparaginyl endopeptidase (AEP)/legumain. Bioorg Med Chem Lett (2006) 1.05

Irreversible inhibition of serine proteases by peptide derivatives of (alpha-aminoalkyl)phosphonate diphenyl esters. Biochemistry (1991) 1.03

Design, synthesis, and evaluation of in vivo potency and selectivity of epoxysuccinyl-based inhibitors of papain-family cysteine proteases. Chem Biol (2007) 1.02

Profiling proteasome activity in tissue with fluorescent probes. Mol Pharm (2007) 1.01

Development of a novel chemical probe for the selective enrichment of phosphorylated serine- and threonine-containing peptides. Chembiochem (2005) 0.98

Assessing enzyme activities using stable isotope labeling and mass spectrometry. Mol Cell Proteomics (2007) 0.98

A cell-permeable inhibitor and activity-based probe for the caspase-like activity of the proteasome. Bioorg Med Chem Lett (2007) 0.96

Advances in clinical cancer proteomics: SELDI-ToF-mass spectrometry and biomarker discovery. Brief Funct Genomic Proteomic (2005) 0.95

Active-site peptide "fingerprinting" of glycosidases in complex mixtures by mass spectrometry. Discovery of a novel retaining beta-1,4-glycanase in Cellulomonas fimi. J Biol Chem (2005) 0.95

Design and synthesis of class-selective activity probes for protein tyrosine phosphatases. J Proteome Res (2003) 0.94

The use of benzyloxycarbonyl[125I]iodotyrosylalanyldiazomethane as a probe for active cysteine proteinases in human tissues. Biochem J (1989) 0.93

Synthesis of acid-cleavable light isotope-coded affinity tags (ICAT-L) for potential use in proteomic expression profiling analysis. Bioconjug Chem (2006) 0.93

Affinity-based tagging of protein families with reversible inhibitors: a concept for functional proteomics. Angew Chem Int Ed Engl (2003) 0.91

Solid-phase synthesis of peptide vinyl sulfones as potential inhibitors and activity-based probes of cysteine proteases. Org Lett (2003) 0.88

Development and characterization of proteasome inhibitors. Methods Enzymol (2005) 0.87

In-cell selectivity profiling of serine protease inhibitors by activity-based proteomics. Mol Cell Proteomics (2008) 0.86

Proteasome inhibitors and antigen presentation. Biopolymers (1997) 0.86

Novel fluorescent phosphonic acid esters for discrimination of lipases and esterases. Chembiochem (2005) 0.86

Development of activity-based probes for trypsin-family serine proteases. Bioorg Med Chem Lett (2006) 0.84

A caspase active site probe reveals high fractional inhibition needed to block DNA fragmentation. J Biol Chem (2004) 0.83

Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases. Bioorg Med Chem Lett (2004) 0.81

Quantitative inhibitor fingerprinting of metalloproteases using small molecule microarrays. J Am Chem Soc (2007) 0.80

"Click" synthesis of small molecule probes for activity-based fingerprinting of matrix metalloproteases. Chem Commun (Camb) (2006) 0.78

A mechanism-based affinity-labeling agent for possible use in isolating N-acetylglucosaminidase. Bioorg Med Chem Lett (2001) 0.77

Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity. Org Biomol Chem (2007) 0.76

Inhibitor fingerprinting of metalloproteases using microplate and microarray platforms: an enabling technology in Catalomics. Nat Protoc (2007) 0.76

Articles by these authors

(truncated to the top 100)

Cathepsin cysteine proteases are effectors of invasive growth and angiogenesis during multistage tumorigenesis. Cancer Cell (2004) 4.38

Identification of proteases that regulate erythrocyte rupture by the malaria parasite Plasmodium falciparum. Nat Chem Biol (2008) 3.16

Dynamic imaging of protease activity with fluorescently quenched activity-based probes. Nat Chem Biol (2005) 2.90

Noninvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes. Nat Chem Biol (2007) 2.80

A cathepsin L isoform that is devoid of a signal peptide localizes to the nucleus in S phase and processes the CDP/Cux transcription factor. Mol Cell (2004) 2.44

Activity-based probes that target diverse cysteine protease families. Nat Chem Biol (2005) 2.21

Tumor cell-derived and macrophage-derived cathepsin B promotes progression and lung metastasis of mammary cancer. Cancer Res (2006) 2.20

A role for the protease falcipain 1 in host cell invasion by the human malaria parasite. Science (2002) 2.20

Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities. J Biol Chem (2006) 2.05

Nucleic acid recognition by Toll-like receptors is coupled to stepwise processing by cathepsins and asparagine endopeptidase. J Exp Med (2011) 1.93

Noninvasive optical imaging of apoptosis by caspase-targeted activity-based probes. Nat Med (2009) 1.90

Cathepsin L in secretory vesicles functions as a prohormone-processing enzyme for production of the enkephalin peptide neurotransmitter. Proc Natl Acad Sci U S A (2003) 1.83

Chemical approaches for functionally probing the proteome. Mol Cell Proteomics (2002) 1.78

Tagging and detection strategies for activity-based proteomics. Curr Opin Chem Biol (2006) 1.70

Small molecule-induced allosteric activation of the Vibrio cholerae RTX cysteine protease domain. Science (2008) 1.67

Autophagic degradation of the BCR-ABL oncoprotein and generation of antileukemic responses by arsenic trioxide. Blood (2012) 1.66

Rational design of inhibitors and activity-based probes targeting Clostridium difficile virulence factor TcdB. Chem Biol (2010) 1.60

Rab35 controls actin bundling by recruiting fascin as an effector protein. Science (2009) 1.51

Regulation of collagenase activities of human cathepsins by glycosaminoglycans. J Biol Chem (2003) 1.46

Activity-based protein profiling: applications to biomarker discovery, in vivo imaging and drug discovery. Am J Pharmacogenomics (2004) 1.45

A substrate-inspired probe monitors translocation, activation, and subcellular targeting of bacterial type III effector protease AvrPphB. Chem Biol (2013) 1.44

Identification of early intermediates of caspase activation using selective inhibitors and activity-based probes. Mol Cell (2006) 1.43

Small molecule affinity fingerprinting. A tool for enzyme family subclassification, target identification, and inhibitor design. Chem Biol (2002) 1.42

Ferri-liposomes as an MRI-visible drug-delivery system for targeting tumours and their microenvironment. Nat Nanotechnol (2011) 1.41

Mechanistic and structural insights into the proteolytic activation of Vibrio cholerae MARTX toxin. Nat Chem Biol (2009) 1.36

Activity profiling of papain-like cysteine proteases in plants. Plant Physiol (2004) 1.36

Live-cell imaging demonstrates extracellular matrix degradation in association with active cathepsin B in caveolae of endothelial cells during tube formation. Exp Cell Res (2009) 1.35

Functional expression and characterization of Schistosoma mansoni cathepsin B and its trans-activation by an endogenous asparaginyl endopeptidase. Mol Biochem Parasitol (2003) 1.34

A mild chemically cleavable linker system for functional proteomic applications. Angew Chem Int Ed Engl (2007) 1.33

Aminopeptidase fingerprints, an integrated approach for identification of good substrates and optimal inhibitors. J Biol Chem (2009) 1.33

VEGF-A induces angiogenesis by perturbing the cathepsin-cysteine protease inhibitor balance in venules, causing basement membrane degradation and mother vessel formation. Cancer Res (2009) 1.32

Functional imaging of proteases: recent advances in the design and application of substrate-based and activity-based probes. Curr Opin Chem Biol (2011) 1.31

Caspase-8 association with the focal adhesion complex promotes tumor cell migration and metastasis. Cancer Res (2009) 1.30

Sequential autolytic processing activates the zymogen of Arg-gingipain. J Biol Chem (2003) 1.27

A nonpeptidic cathepsin S activity-based probe for noninvasive optical imaging of tumor-associated macrophages. Chem Biol (2012) 1.27

Inhibition of cysteine cathepsin protease activity enhances chemotherapy regimens by decreasing tumor growth and invasiveness in a mouse model of multistage cancer. Cancer Res (2007) 1.24

Cathepsin V, a novel and potent elastolytic activity expressed in activated macrophages. J Biol Chem (2004) 1.24

Commonly used caspase inhibitors designed based on substrate specificity profiles lack selectivity. Cell Res (2006) 1.22

Functional studies of Plasmodium falciparum dipeptidyl aminopeptidase I using small molecule inhibitors and active site probes. Chem Biol (2010) 1.20

Targeted disruption of Plasmodium falciparum cysteine protease, falcipain 1, reduces oocyst production, not erythrocytic stage growth. Mol Microbiol (2004) 1.19

Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag. PLoS One (2009) 1.19

Increased expression and activity of nuclear cathepsin L in cancer cells suggests a novel mechanism of cell transformation. Mol Cancer Res (2007) 1.18

Hemoglobin digestion in blood-feeding ticks: mapping a multipeptidase pathway by functional proteomics. Chem Biol (2009) 1.17

Application of activity-based probes to the study of enzymes involved in cancer progression. Curr Opin Genet Dev (2008) 1.17

Inhibition of cathepsin B reduces beta-amyloid production in regulated secretory vesicles of neuronal chromaffin cells: evidence for cathepsin B as a candidate beta-secretase of Alzheimer's disease. Biol Chem (2005) 1.16

Falstatin, a cysteine protease inhibitor of Plasmodium falciparum, facilitates erythrocyte invasion. PLoS Pathog (2006) 1.15

Proteomic analysis of fractionated Toxoplasma oocysts reveals clues to their environmental resistance. PLoS One (2012) 1.15

Engineered hybrid dimers: tracking the activation pathway of caspase-7. Mol Cell (2006) 1.14

Using small molecules to dissect mechanisms of microbial pathogenesis. ACS Chem Biol (2009) 1.14

Improved quenched fluorescent probe for imaging of cysteine cathepsin activity. J Am Chem Soc (2013) 1.14

Development of near-infrared fluorophore (NIRF)-labeled activity-based probes for in vivo imaging of legumain. ACS Chem Biol (2010) 1.13

New approaches for dissecting protease functions to improve probe development and drug discovery. Nat Struct Mol Biol (2012) 1.11

Activity-based profiling of proteases. Annu Rev Biochem (2014) 1.10

Topical application of activity-based probes for visualization of brain tumor tissue. PLoS One (2012) 1.10

Activity based probes for proteases: applications to biomarker discovery, molecular imaging and drug screening. Curr Pharm Des (2007) 1.10

Coupling protein engineering with probe design to inhibit and image matrix metalloproteinases with controlled specificity. J Am Chem Soc (2013) 1.10

A coupled protein and probe engineering approach for selective inhibition and activity-based probe labeling of the caspases. J Am Chem Soc (2013) 1.09

A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C. J Am Chem Soc (2006) 1.09

Validation of the proteasome as a therapeutic target in Plasmodium using an epoxyketone inhibitor with parasite-specific toxicity. Chem Biol (2012) 1.09

Comparative assessment of substrates and activity based probes as tools for non-invasive optical imaging of cysteine protease activity. PLoS One (2009) 1.08

Proteomics evaluation of chemically cleavable activity-based probes. Mol Cell Proteomics (2007) 1.08

Target deconvolution techniques in modern phenotypic profiling. Curr Opin Chem Biol (2013) 1.08

Cysteine protease inhibitors block Toxoplasma gondii microneme secretion and cell invasion. Antimicrob Agents Chemother (2006) 1.06

Functional imaging of legumain in cancer using a new quenched activity-based probe. J Am Chem Soc (2012) 1.05

Design of cell-permeable, fluorescent activity-based probes for the lysosomal cysteine protease asparaginyl endopeptidase (AEP)/legumain. Bioorg Med Chem Lett (2006) 1.05

Enzyme activity--it's all about image. Trends Cell Biol (2004) 1.05

Biochemical analysis of the 20 S proteasome of Trypanosoma brucei. J Biol Chem (2003) 1.04

Global profiling of proteolysis during rupture of Plasmodium falciparum from the host erythrocyte. Mol Cell Proteomics (2010) 1.04

Treatment of arthritis by macrophage depletion and immunomodulation: testing an apoptosis-mediated therapy in a humanized death receptor mouse model. Arthritis Rheum (2011) 1.03

Cathepsin S is activated during colitis and causes visceral hyperalgesia by a PAR2-dependent mechanism in mice. Gastroenterology (2011) 1.03

Autocatalytic processing of procathepsin B is triggered by proenzyme activity. FEBS J (2009) 1.02

Caspase-1 activity is required to bypass macrophage apoptosis upon Salmonella infection. Nat Chem Biol (2012) 1.02

Design, synthesis, and evaluation of in vivo potency and selectivity of epoxysuccinyl-based inhibitors of papain-family cysteine proteases. Chem Biol (2007) 1.02

Chemical proteomics and its application to drug discovery. Curr Opin Biotechnol (2003) 1.02

Ubiquitin-like modifiers and their deconjugating enzymes in medically important parasitic protozoa. Eukaryot Cell (2007) 1.02

Toxoplasma gondii cathepsin L is the primary target of the invasion-inhibitory compound morpholinurea-leucyl-homophenyl-vinyl sulfone phenyl. J Biol Chem (2009) 1.02

The role of cathepsin X in the migration and invasiveness of T lymphocytes. J Cell Sci (2008) 1.01

Probing structural determinants distal to the site of hydrolysis that control substrate specificity of the 20S proteasome. Chem Biol (2002) 1.01

IrAE: an asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus. Int J Parasitol (2007) 1.01

Caspase-3 feeds back on caspase-8, Bid and XIAP in type I Fas signaling in primary mouse hepatocytes. Apoptosis (2012) 0.99

Defining an allosteric circuit in the cysteine protease domain of Clostridium difficile toxins. Nat Struct Mol Biol (2011) 0.99

Subclassification and biochemical analysis of plant papain-like cysteine proteases displays subfamily-specific characteristics. Plant Physiol (2012) 0.99

Functional characterization of a SUMO deconjugating protease of Plasmodium falciparum using newly identified small molecule inhibitors. Chem Biol (2011) 0.99

Trial of the cysteine cathepsin inhibitor JPM-OEt on early and advanced mammary cancer stages in the MMTV-PyMT-transgenic mouse model. Biol Chem (2008) 0.98

Cathepsin L and Arg/Lys aminopeptidase: a distinct prohormone processing pathway for the biosynthesis of peptide neurotransmitters and hormones. Biol Chem (2004) 0.98

Characteristics of the caspase-like catalytic domain of human paracaspase. Biol Chem (2004) 0.98

An optimized activity-based probe for the study of caspase-6 activation. Chem Biol (2012) 0.97

Non-invasive imaging of cysteine cathepsin activity in solid tumors using a 64Cu-labeled activity-based probe. PLoS One (2011) 0.97

Acid-mediated tumor proteolysis: contribution of cysteine cathepsins. Neoplasia (2013) 0.96

Design, syntheses, and evaluation of Taspase1 inhibitors. Bioorg Med Chem Lett (2009) 0.96

Chemical genetic screen identifies Toxoplasma DJ-1 as a regulator of parasite secretion, attachment, and invasion. Proc Natl Acad Sci U S A (2011) 0.95

Cathepsin X is secreted by human osteoblasts, digests CXCL-12 and impairs adhesion of hematopoietic stem and progenitor cells to osteoblasts. Haematologica (2010) 0.94

Small-molecule inhibition of a depalmitoylase enhances Toxoplasma host-cell invasion. Nat Chem Biol (2013) 0.93

A fragmenting hybrid approach for targeted delivery of multiple therapeutic agents to the malaria parasite. ChemMedChem (2011) 0.93

Substrate specificity of schistosome versus human legumain determined by P1-P3 peptide libraries. Mol Biochem Parasitol (2002) 0.92

Minitags for small molecules: detecting targets of reactive small molecules in living plant tissues using 'click chemistry'. Plant J (2008) 0.91

4-Bromophenacyl bromide specifically inhibits rhoptry secretion during Toxoplasma invasion. PLoS One (2009) 0.91

Assessing subunit dependency of the Plasmodium proteasome using small molecule inhibitors and active site probes. ACS Chem Biol (2014) 0.90

Identification of a serine protease inhibitor which causes inclusion vacuole reduction and is lethal to Chlamydia trachomatis. Mol Microbiol (2013) 0.90

Proteases as regulators of pathogenesis: examples from the Apicomplexa. Biochim Biophys Acta (2011) 0.90