Sites of interaction of the CCA end of peptidyl-tRNA with 23S rRNA.

PubWeight™: 2.34‹?› | Rank: Top 2%

🔗 View Article (PMC 51525)

Published in Proc Natl Acad Sci U S A on May 01, 1991

Authors

D Moazed1, H F Noller

Author Affiliations

1: Sinsheimer Laboratories, University of California, Santa Cruz 95064.

Articles citing this

Structural insights into peptide bond formation. Proc Natl Acad Sci U S A (2002) 2.40

Base pairing between Escherichia coli RNase P RNA and its substrate. EMBO J (1994) 2.26

Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action. J Bacteriol (2000) 2.25

Quality control by the ribosome following peptide bond formation. Nature (2008) 2.15

Analysis of mutations at residues A2451 and G2447 of 23S rRNA in the peptidyltransferase active site of the 50S ribosomal subunit. Proc Natl Acad Sci U S A (2001) 2.06

DMS footprinting of structured RNAs and RNA-protein complexes. Nat Protoc (2007) 1.82

Phylogenetic comparative chemical footprint analysis of the interaction between ribonuclease P RNA and tRNA. EMBO J (1994) 1.76

Functional interactions within 23S rRNA involving the peptidyltransferase center. J Bacteriol (1992) 1.74

Alterations at the peptidyl transferase centre of the ribosome induced by the synergistic action of the streptogramins dalfopristin and quinupristin. BMC Biol (2004) 1.68

Complete genome of the broad-host-range Erwinia amylovora phage phiEa21-4 and its relationship to Salmonella phage felix O1. Appl Environ Microbiol (2009) 1.67

Optimization of ribosome structure and function by rRNA base modification. PLoS One (2007) 1.57

A conformational change in the ribosomal peptidyl transferase center upon active/inactive transition. Proc Natl Acad Sci U S A (2001) 1.57

Quantitative dimethyl sulfate mapping for automated RNA secondary structure inference. Biochemistry (2012) 1.37

The "DEAD box" protein DbpA interacts specifically with the peptidyltransferase center in 23S rRNA. Proc Natl Acad Sci U S A (1995) 1.35

Hydroxyl radical cleavage of tRNA in the ribosomal P site. Proc Natl Acad Sci U S A (1992) 1.34

Functional tRNAs with altered 3' ends. EMBO J (1993) 1.24

Many of the conserved nucleotides of tRNA(Phe) are not essential for ternary complex formation and peptide elongation. EMBO J (1994) 1.24

23S rRNA positions essential for tRNA binding in ribosomal functional sites. Proc Natl Acad Sci U S A (1998) 1.22

Mapping the rRNA neighborhood of the acceptor end of tRNA in the ribosome. EMBO J (1996) 1.22

Mutations in the peptidyl transferase region of E. coli 23S rRNA affecting translational accuracy. Nucleic Acids Res (1994) 1.19

A new view of protein synthesis: mapping the free energy landscape of the ribosome using single-molecule FRET. Biopolymers (2008) 1.16

Evidence for functional interaction between elongation factor Tu and 16S ribosomal RNA. Proc Natl Acad Sci U S A (1993) 1.13

Evolution of protein synthesis from an RNA world. Cold Spring Harb Perspect Biol (2012) 1.12

Site-directed cross-linking studies on the E. coli tRNA-ribosome complex: determination of sites labelled with an aromatic azide attached to the variable loop or aminoacyl group of tRNA. Nucleic Acids Res (1993) 1.09

Peptidyl transferase: protein, ribonucleoprotein, or RNA? J Bacteriol (1993) 1.09

A structural view on the mechanism of the ribosome-catalyzed peptide bond formation. Biochim Biophys Acta (2009) 1.08

Non-coding RNAs: multi-tasking molecules in the cell. Int J Mol Sci (2013) 1.07

Direct crosslinking of the antitumor antibiotic sparsomycin, and its derivatives, to A2602 in the peptidyl transferase center of 23S-like rRNA within ribosome-tRNA complexes. Proc Natl Acad Sci U S A (1999) 1.06

Delayed rRNA processing results in significant ribosome biogenesis and functional defects. Mol Cell Biol (2003) 1.05

Substitution of the 3' terminal adenosine residue of transfer RNA in vivo. Proc Natl Acad Sci U S A (1993) 1.01

How are tRNAs and mRNA arranged in the ribosome? An attempt to correlate the stereochemistry of the tRNA-mRNA interaction with constraints imposed by the ribosomal topography. Nucleic Acids Res (1992) 1.01

Protein folding by domain V of Escherichia coli 23S rRNA: specificity of RNA-protein interactions. J Bacteriol (2008) 1.00

High throughput structural analysis of yeast ribosomes using hSHAPE. RNA Biol (2011) 0.99

Novel mutants of 23S RNA: characterization of functional properties. Nucleic Acids Res (1992) 0.98

Mapping the path of the nascent peptide chain through the 23S RNA in the 50S ribosomal subunit. Nucleic Acids Res (1995) 0.95

Reverse splicing of the Tetrahymena IVS: evidence for multiple reaction sites in the 23S rRNA. RNA (1995) 0.94

Peptidyl transferase antibiotics perturb the relative positioning of the 3'-terminal adenosine of P/P'-site-bound tRNA and 23S rRNA in the ribosome. RNA (1999) 0.94

Exploration of the conserved A+C wobble pair within the ribosomal peptidyl transferase center using affinity purified mutant ribosomes. Nucleic Acids Res (2004) 0.93

Role for the highly conserved region of domain IV of 23S-like rRNA in subunit-subunit interactions at the peptidyl transferase centre. Nucleic Acids Res (1995) 0.93

Contacts between the growing peptide chain and the 23S RNA in the 50S ribosomal subunit. Nucleic Acids Res (1994) 0.93

The role of SmpB and the ribosomal decoding center in licensing tmRNA entry into stalled ribosomes. RNA (2011) 0.90

Functional transfer RNAs with modifications in the 3'-CCA end: differential effects on aminoacylation and polypeptide synthesis. Proc Natl Acad Sci U S A (1994) 0.89

Surprising contribution to aminoacylation and translation of non-Watson-Crick pairs in tRNA. Proc Natl Acad Sci U S A (2006) 0.89

Interactions between 23S rRNA and tRNA in the ribosomal E site. RNA (2001) 0.89

ArfA recognizes the lack of mRNA in the mRNA channel after RF2 binding for ribosome rescue. Nucleic Acids Res (2014) 0.86

Evidence against stabilization of the transition state oxyanion by a pKa-perturbed RNA base in the peptidyl transferase center. Proc Natl Acad Sci U S A (2002) 0.85

Toward ribosomal RNA catalytic activity in the absence of protein. J Mol Evol (2007) 0.84

Deregulation of poly(A) polymerase I in Escherichia coli inhibits protein synthesis and leads to cell death. Nucleic Acids Res (2012) 0.82

Chemical probing of a virginiamycin M-promoted conformational change of the peptidyl-transferase domain. Nucleic Acids Res (1994) 0.82

Solid phase synthesis and binding affinity of peptidyl transferase transition state mimics containing 2'-OH at P-site position A76. Nucleic Acids Res (2004) 0.80

Rejection of tmRNA·SmpB after GTP hydrolysis by EF-Tu on ribosomes stalled on intact mRNA. RNA (2014) 0.80

3'-immature tRNA(Trp) is required for ribosome inactivation by gelonin,a plant RNA N-glycosidase. Biochem J (1995) 0.78

Recent developments of engineered translational machineries for the incorporation of non-canonical amino acids into polypeptides. Int J Mol Sci (2015) 0.76

Mutational analysis of the donor substrate binding site of the ribosomal peptidyltransferase center. RNA (1998) 0.76

An intragenic suppressor of cold sensitivity identifies potentially interacting bases in the peptidyl transferase center of Tetrahymena rRNA. Genetics (1998) 0.76

Articles cited by this

Interaction of antibiotics with functional sites in 16S ribosomal RNA. Nature (1987) 8.96

Intermediate states in the movement of transfer RNA in the ribosome. Nature (1989) 7.30

Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension. Methods Enzymol (1988) 6.38

Interaction of tRNA with 23S rRNA in the ribosomal A, P, and E sites. Cell (1989) 5.79

Transfer RNA shields specific nucleotides in 16S ribosomal RNA from attack by chemical probes. Cell (1986) 4.65

Altered ribosomal RNA genes in mitochondria from mammalian cells with chloramphenicol resistance. Nature (1981) 4.65

Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA. Biochimie (1987) 4.47

A compilation of large subunit RNA sequences presented in a structural format. Nucleic Acids Res (1988) 4.38

The behaviour of acetylphenylalanyl soluble ribonucleic acid in polyphenylalanine synthesis. Biochim Biophys Acta (1966) 3.30

Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16 S rRNA. J Mol Biol (1990) 2.90

Covalent crosslinking of tRNA1Val to 16S RNA at the ribosomal P site: identification of crosslinked residues. Proc Natl Acad Sci U S A (1982) 2.87

Identification of a site on 23S ribosomal RNA located at the peptidyl transferase center. Proc Natl Acad Sci U S A (1984) 2.22

The importance of highly conserved nucleotides in the binding region of chloramphenicol at the peptidyl transfer centre of Escherichia coli 23S ribosomal RNA. EMBO J (1988) 2.07

Photo-affinity labelling at the peptidyl transferase centre reveals two different positions for the A- and P-sites in domain V of 23S rRNA. EMBO J (1988) 1.97

Ribosome-catalyzed peptidyl transfer: substrate specificity at the P-site. Proc Natl Acad Sci U S A (1968) 1.65

Action of sparsomycin on ribosome-catalysed peptidyl transfer. Nature (1969) 1.40

An apparent conformational change in tRNA(Phe) that is associated with the peptidyl transferase reaction. Biochimie (1987) 0.91

Articles by these authors

(truncated to the top 100)

Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A (1978) 32.65

Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol (1981) 29.04

Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiol Rev (1983) 23.08

Crystal structure of the ribosome at 5.5 A resolution. Science (2001) 18.21

Comparative anatomy of 16-S-like ribosomal RNA. Prog Nucleic Acid Res Mol Biol (1985) 16.21

X-ray crystal structures of 70S ribosome functional complexes. Science (1999) 10.51

Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence. Nucleic Acids Res (1980) 9.50

Structure of ribosomal RNA. Annu Rev Biochem (1984) 9.44

Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid (1981) 9.00

Interaction of antibiotics with functional sites in 16S ribosomal RNA. Nature (1987) 8.96

Rapid chemical probing of conformation in 16 S ribosomal RNA and 30 S ribosomal subunits using primer extension. J Mol Biol (1986) 7.87

Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A (1980) 7.80

Intermediate states in the movement of transfer RNA in the ribosome. Nature (1989) 7.30

Secondary structure model for 23S ribosomal RNA. Nucleic Acids Res (1981) 7.12

Secondary structure of 16S ribosomal RNA. Science (1981) 6.52

Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension. Methods Enzymol (1988) 6.38

Interaction of tRNA with 23S rRNA in the ribosomal A, P, and E sites. Cell (1989) 5.79

Protection of specific sites in 16 S RNA from chemical modification by association of 30 S and 50 S ribosomes. J Mol Biol (1977) 5.66

The path of messenger RNA through the ribosome. Cell (2001) 5.65

Mechanism of ribosomal subunit association: discrimination of specific sites in 16 S RNA essential for association activity. J Mol Biol (1979) 4.88

rII cistrons of bacteriophage T4. DNA sequence around the intercistronic divide and positions of genetic landmarks. J Mol Biol (1981) 4.76

Transfer RNA shields specific nucleotides in 16S ribosomal RNA from attack by chemical probes. Cell (1986) 4.65

A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome. Nature (1995) 4.59

Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA. Nature (1988) 4.53

Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA. Biochimie (1987) 4.47

The nucleotide sequence of a rat 18 S ribosomal ribonucleic acid gene and a proposal for the secondary structure of 18 S ribosomal ribonucleic acid. J Biol Chem (1984) 4.00

Ribosomes and translation. Annu Rev Biochem (1997) 3.87

A functional pseudoknot in 16S ribosomal RNA. EMBO J (1991) 3.59

Identification of an RNA-protein bridge spanning the ribosomal subunit interface. Science (1999) 3.31

Model for the three-dimensional folding of 16 S ribosomal RNA. J Mol Biol (1988) 3.24

A "bulged" double helix in a RNA-protein contact site. Proc Natl Acad Sci U S A (1981) 3.16

RNA-protein interactions in 30S ribosomal subunits: folding and function of 16S rRNA. Science (1989) 2.97

Higher order structure in ribosomal RNA. EMBO J (1986) 2.97

Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16 S rRNA. J Mol Biol (1990) 2.90

Functional modification of 16S ribosomal RNA by kethoxal. Proc Natl Acad Sci U S A (1972) 2.74

Topography of 16S RNA in 30S ribosomal subunits. Nucleotide sequences and location of sites of reaction with kethoxal. Biochemistry (1974) 2.67

Mutations in 16S ribosomal RNA disrupt antibiotic--RNA interactions. EMBO J (1989) 2.54

Altered topography of 16S RNA in the inactive form of Escherichia coli 30S ribosomal subunits. Biochemistry (1978) 2.44

Amino-acid sequence of glyceraldehyde 3-phosphate dehydrogenase from lobster muscle. Nature (1967) 2.40

Interaction of translation initiation factor 3 with the 30S ribosomal subunit. Mol Cell (2001) 2.34

The structure of the RNA binding site of ribosomal proteins S8 and S15. J Biol Chem (1979) 2.24

Identification of a site on 23S ribosomal RNA located at the peptidyl transferase center. Proc Natl Acad Sci U S A (1984) 2.22

Nucleotide sequence of the 3' terminus of E. coli 16S ribosomal RNA. Mol Biol Rep (1974) 2.13

A fragment of 23S RNA containing a nucleotide sequence complementary to a region of 5S RNA. FEBS Lett (1975) 2.07

Analysis of mutations at residues A2451 and G2447 of 23S rRNA in the peptidyltransferase active site of the 50S ribosomal subunit. Proc Natl Acad Sci U S A (2001) 2.06

Aminoacyl esterase activity of the Tetrahymena ribozyme. Science (1992) 2.06

Mapping the position of translational elongation factor EF-G in the ribosome by directed hydroxyl radical probing. Cell (1998) 2.06

Dynamics of in vitro assembly of 16 S rRNA into 30 S ribosomal subunits. J Mol Biol (1993) 2.02

A cold-sensitive mutation in 16S rRNA provides evidence for helical switching in ribosome assembly. Genes Dev (1993) 2.00

Hydroxyl radical footprinting of ribosomal proteins on 16S rRNA. RNA (1995) 1.98

Interconversion of active and inactive 30 S ribosomal subunits is accompanied by a conformational change in the decoding region of 16 S rRNA. J Mol Biol (1986) 1.97

Interaction of antibiotics with A- and P-site-specific bases in 16S ribosomal RNA. EMBO J (1991) 1.96

Mutations in ribosomal proteins S4 and S12 influence the higher order structure of 16 S ribosomal RNA. J Mol Biol (1989) 1.94

Dominant lethal mutations in a conserved loop in 16S rRNA. Proc Natl Acad Sci U S A (1990) 1.91

Footprinting the sites of interaction of antibiotics with catalytic group I intron RNA. Science (1993) 1.90

In vitro complementation analysis localizes 23S rRNA posttranscriptional modifications that are required for Escherichia coli 50S ribosomal subunit assembly and function. RNA (1996) 1.85

Footprinting mRNA-ribosome complexes with chemical probes. EMBO J (1994) 1.84

Localization of the binding site for protein S4 on 16 S ribosomal RNA by chemical and enzymatic probing and primer extension. J Mol Biol (1986) 1.83

Isolation of temperature-sensitive mutants of 16 S rRNA in Escherichia coli. J Mol Biol (1989) 1.82

Protection of specific sites in 23 S and 5 S RNA from chemical modification by association of 30 S and 50 S ribosomes. J Mol Biol (1979) 1.82

Phylogenetic comparative chemical footprint analysis of the interaction between ribonuclease P RNA and tRNA. EMBO J (1994) 1.76

Specific protection of 16 S rRNA by translational initiation factors. J Mol Biol (1995) 1.76

Molecular basis of tetracycline action: identification of analogs whose primary target is not the bacterial ribosome. Antimicrob Agents Chemother (1991) 1.69

EF-G-catalyzed translocation of anticodon stem-loop analogs of transfer RNA in the ribosome. EMBO J (1998) 1.68

Structure of 5 S ribosomal RNA from Escherichia coli: identification of kethoxal-reactive sites in the A and B conformations. J Mol Biol (1979) 1.66

Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA. Genes Dev (1995) 1.66

Interaction of ribosomal proteins S5, S6, S11, S12, S18 and S21 with 16 S rRNA. J Mol Biol (1988) 1.63

Efficient reconstitution of functional Escherichia coli 30S ribosomal subunits from a complete set of recombinant small subunit ribosomal proteins. RNA (1999) 1.60

Defining the structural requirements for a helix in 23 S ribosomal RNA that confers erythromycin resistance. J Mol Biol (1989) 1.57

Molecular movement inside the translational engine. Cell (1998) 1.53

Alteration of polynucleotide secondary structure by ribosomal protein S1. Proc Natl Acad Sci U S A (1976) 1.49

Reconstitution of functional 50S ribosomes from in vitro transcripts of Bacillus stearothermophilus 23S rRNA. Biochemistry (1999) 1.47

Identification of bases in 16S rRNA essential for tRNA binding at the 30S ribosomal P site. Science (1995) 1.46

Structures of complexes of 5S RNA with ribosomal proteins L5, L18 and L25 from Escherichia coli: identification of kethoxal-reactive sites on the 5S RNA. J Mol Biol (1979) 1.45

Independent in vitro assembly of a ribonucleoprotein particle containing the 3' domain of 16S rRNA. Proc Natl Acad Sci U S A (1994) 1.43

Mutations at nucleotides G2251 and U2585 of 23 S rRNA perturb the peptidyl transferase center of the ribosome. J Mol Biol (1997) 1.34

Hydroxyl radical cleavage of tRNA in the ribosomal P site. Proc Natl Acad Sci U S A (1992) 1.34

Protection of ribosomal RNA from kethoxal in polyribosomes. Implication of specific sites in ribosome function. J Mol Biol (1983) 1.33

Nucleotides in 16S rRNA protected by the association of 30S and 50S ribosomal subunits. J Mol Biol (1999) 1.32

Mapping the inside of the ribosome with an RNA helical ruler. Science (1997) 1.31

Interaction of proteins S16, S17 and S20 with 16 S ribosomal RNA. J Mol Biol (1988) 1.29

In vitro reconstitution of 30S ribosomal subunits using complete set of recombinant proteins. Methods Enzymol (2000) 1.29

Probing the conformation of 18S rRNA in yeast 40S ribosomal subunits with kethoxal. Biochemistry (1984) 1.28

Ribosome-catalyzed peptide-bond formation with an A-site substrate covalently linked to 23S ribosomal RNA. Science (1998) 1.27

Directed hydroxyl radical probing of 16S rRNA using Fe(II) tethered to ribosomal protein S4. Proc Natl Acad Sci U S A (1995) 1.27

Directed hydroxyl radical probing of RNA from iron(II) tethered to proteins in ribonucleoprotein complexes. Methods Enzymol (2000) 1.26

Interaction of ribosomal proteins, S6, S8, S15 and S18 with the central domain of 16 S ribosomal RNA. J Mol Biol (1988) 1.26

Complete nucleotide sequence of a 23S ribosomal RNA gene from Bacillus stearothermophilus. DNA (1984) 1.25

Identification of a site of psoralen crosslinking in E. coli 16S ribosomal RNA. Nucleic Acids Res (1982) 1.24

Identification of a ribosomal protein essential for peptidyl transferase activity. Proc Natl Acad Sci U S A (1975) 1.24

Selective perturbation of G530 of 16 S rRNA by translational miscoding agents and a streptomycin-dependence mutation in protein S12. J Mol Biol (1994) 1.24

Mapping the rRNA neighborhood of the acceptor end of tRNA in the ribosome. EMBO J (1996) 1.22

Nucleotides in 23S rRNA protected by the association of 30S and 50S ribosomal subunits. J Mol Biol (1999) 1.21

Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli. Mol Gen Genet (1979) 1.20

Probing the assembly of the 3' major domain of 16 S ribosomal RNA. Quaternary interactions involving ribosomal proteins S7, S9 and S19. J Mol Biol (1988) 1.19

Nucleotide sequences of accessible regions of 23S RNA in 50S ribosomal subunits. Biochemistry (1978) 1.18

Characterization of functionally active subribosomal particles from Thermus aquaticus. Proc Natl Acad Sci U S A (1999) 1.15

Evidence for functional interaction between domains II and V of 23S ribosomal RNA from an erythromycin-resistant mutant. Proc Natl Acad Sci U S A (1985) 1.13

Evidence for functional interaction between elongation factor Tu and 16S ribosomal RNA. Proc Natl Acad Sci U S A (1993) 1.13

Identification of 2'-hydroxyl groups required for interaction of a tRNA anticodon stem-loop region with the ribosome. RNA (1997) 1.11