Published in Methods Mol Biol on January 01, 2010
A TALE nuclease architecture for efficient genome editing. Nat Biotechnol (2010) 26.47
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell (2013) 24.00
DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol (2013) 19.99
Genome engineering using the CRISPR-Cas9 system. Nat Protoc (2013) 15.98
FLASH assembly of TALENs for high-throughput genome editing. Nat Biotechnol (2012) 12.97
One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell (2013) 7.99
A transcription activator-like effector toolbox for genome engineering. Nat Protoc (2012) 6.81
An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nat Biotechnol (2011) 6.44
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures. Nat Methods (2010) 6.00
Efficient TALEN-mediated gene knockout in livestock. Proc Natl Acad Sci U S A (2012) 4.99
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells. Nature (2011) 4.92
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity. Nucleic Acids Res (2013) 4.11
Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol (2014) 2.75
Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases. Proc Natl Acad Sci U S A (2011) 2.61
CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences. Nucleic Acids Res (2014) 2.59
TP53 loss creates therapeutic vulnerability in colorectal cancer. Nature (2015) 2.33
Efficient nonmeiotic allele introgression in livestock using custom endonucleases. Proc Natl Acad Sci U S A (2013) 2.22
Targeted gene knockout by direct delivery of zinc-finger nuclease proteins. Nat Methods (2012) 2.07
Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme. Genome Res (2012) 2.02
Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Genome Res (2014) 1.91
TALEN-based gene correction for epidermolysis bullosa. Mol Ther (2013) 1.84
Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep (2014) 1.83
An iCRISPR platform for rapid, multiplexable, and inducible genome editing in human pluripotent stem cells. Cell Stem Cell (2014) 1.83
A mouse geneticist's practical guide to CRISPR applications. Genetics (2014) 1.81
Simultaneous zinc-finger nuclease editing of the HIV coreceptors ccr5 and cxcr4 protects CD4+ T cells from HIV-1 infection. Blood (2013) 1.80
Generating genetically modified mice using CRISPR/Cas-mediated genome engineering. Nat Protoc (2014) 1.76
Translating dosage compensation to trisomy 21. Nature (2013) 1.75
Forward genetic screen for malignant peripheral nerve sheath tumor formation identifies new genes and pathways driving tumorigenesis. Nat Genet (2013) 1.60
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res (2014) 1.59
Increasing frequencies of site-specific mutagenesis and gene targeting in Arabidopsis by manipulating DNA repair pathways. Genome Res (2013) 1.57
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Cell Rep (2014) 1.50
Genomic editing of the HIV-1 coreceptor CCR5 in adult hematopoietic stem and progenitor cells using zinc finger nucleases. Mol Ther (2013) 1.49
Generation of knock-in primary human T cells using Cas9 ribonucleoproteins. Proc Natl Acad Sci U S A (2015) 1.49
The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo. Mol Ther Nucleic Acids (2014) 1.46
Cancer translocations in human cells induced by zinc finger and TALE nucleases. Genome Res (2013) 1.44
Targeting Hepatitis B Virus With CRISPR/Cas9. Mol Ther Nucleic Acids (2014) 1.33
COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites. Mol Ther Nucleic Acids (2014) 1.27
TALEN-mediated editing of the mouse Y chromosome. Nat Biotechnol (2013) 1.26
Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases. Nucleic Acids Res (2011) 1.25
Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering. PLoS One (2014) 1.24
Nuclease-mediated gene editing by homologous recombination of the human globin locus. Nucleic Acids Res (2013) 1.22
Targeted mutagenesis of Arabidopsis thaliana using engineered TAL effector nucleases. G3 (Bethesda) (2013) 1.19
Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling. Nat Commun (2015) 1.17
Highly active zinc-finger nucleases by extended modular assembly. Genome Res (2012) 1.17
Toward eliminating HLA class I expression to generate universal cells from allogeneic donors. Blood (2013) 1.17
Actin and dynamin2 dynamics and interplay during clathrin-mediated endocytosis. J Cell Biol (2014) 1.15
Quantifying on- and off-target genome editing. Trends Biotechnol (2015) 1.15
TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis. Proc Natl Acad Sci U S A (2015) 1.14
Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) (2015) 1.13
Efficient targeted mutagenesis in the monarch butterfly using zinc-finger nucleases. Genome Res (2012) 1.12
Genetic and chemical correction of cholesterol accumulation and impaired autophagy in hepatic and neural cells derived from Niemann-Pick Type C patient-specific iPS cells. Stem Cell Reports (2014) 1.11
Zinc-finger nuclease editing of human cxcr4 promotes HIV-1 CD4(+) T cell resistance and enrichment. Mol Ther (2012) 1.10
Correction of dystrophin expression in cells from Duchenne muscular dystrophy patients through genomic excision of exon 51 by zinc finger nucleases. Mol Ther (2014) 1.09
Integrase-defective lentiviral vectors as a delivery platform for targeted modification of adenosine deaminase locus. Mol Ther (2013) 1.08
Global analysis of Drosophila Cys₂-His₂ zinc finger proteins reveals a multitude of novel recognition motifs and binding determinants. Genome Res (2013) 1.07
Functional footprinting of regulatory DNA. Nat Methods (2015) 1.04
Evaluation of TCR Gene Editing Achieved by TALENs, CRISPR/Cas9, and megaTAL Nucleases. Mol Ther (2015) 1.04
Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells. Cell Rep (2015) 1.00
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice. Nat Commun (2016) 1.00
Correction of human phospholamban R14del mutation associated with cardiomyopathy using targeted nucleases and combination therapy. Nat Commun (2015) 0.99
The major cellular sterol regulatory pathway is required for Andes virus infection. PLoS Pathog (2014) 0.98
Design and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus. Mol Ther Nucleic Acids (2013) 0.97
SAPTA: a new design tool for improving TALE nuclease activity. Nucleic Acids Res (2014) 0.96
Correction of the ΔF508 Mutation in the Cystic Fibrosis Transmembrane Conductance Regulator Gene by Zinc-Finger Nuclease Homology-Directed Repair. Biores Open Access (2012) 0.95
Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies. Nat Commun (2015) 0.94
Amphipathic α-helices in apolipoproteins are crucial to the formation of infectious hepatitis C virus particles. PLoS Pathog (2014) 0.93
Real-time resolution of point mutations that cause phenovariance in mice. Proc Natl Acad Sci U S A (2015) 0.93
DrugTargetSeqR: a genomics- and CRISPR-Cas9-based method to analyze drug targets. Nat Chem Biol (2014) 0.93
Targeted deletion and inversion of tandemly arrayed genes in Arabidopsis thaliana using zinc finger nucleases. G3 (Bethesda) (2013) 0.92
Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity. Mol Cell (2016) 0.91
Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development (2014) 0.90
CRISPR/cas9, a novel genomic tool to knock down microRNA in vitro and in vivo. Sci Rep (2016) 0.90
Cas9-based genome editing in Xenopus tropicalis. Methods Enzymol (2014) 0.89
Mammalian interspecies substitution of immune modulatory alleles by genome editing. Sci Rep (2016) 0.89
TALEN and CRISPR/Cas Genome Editing Systems: Tools of Discovery. Acta Naturae (2014) 0.89
Biallelic targeting of expressed genes in mouse embryonic stem cells using the Cas9 system. Methods (2014) 0.88
GPR107, a G-protein-coupled receptor essential for intoxication by Pseudomonas aeruginosa exotoxin A, localizes to the Golgi and is cleaved by furin. J Biol Chem (2014) 0.88
Pooled CRISPR screening with single-cell transcriptome readout. Nat Methods (2017) 0.87
Fanconi anemia gene editing by the CRISPR/Cas9 system. Hum Gene Ther (2015) 0.87
Simple and efficient methods for enrichment and isolation of endonuclease modified cells. PLoS One (2014) 0.87
High-throughput genotyping of CRISPR/Cas9-mediated mutants using fluorescent PCR-capillary gel electrophoresis. Sci Rep (2015) 0.87
Redesign of extensive protein-DNA interfaces of meganucleases using iterative cycles of in vitro compartmentalization. Proc Natl Acad Sci U S A (2014) 0.86
Targeted genome engineering in human induced pluripotent stem cells by penetrating TALENs. Cell Regen (Lond) (2013) 0.86
Rh D blood group conversion using transcription activator-like effector nucleases. Nat Commun (2015) 0.86
Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering. PLoS One (2015) 0.85
Efficient Genome Editing in Chicken DF-1 Cells Using the CRISPR/Cas9 System. G3 (Bethesda) (2016) 0.85
Synthetic zinc finger nuclease design and rapid assembly. Hum Gene Ther (2011) 0.85
Clinical Scale Zinc Finger Nuclease-mediated Gene Editing of PD-1 in Tumor Infiltrating Lymphocytes for the Treatment of Metastatic Melanoma. Mol Ther (2015) 0.84
Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide RNAs. Nat Commun (2014) 0.84
Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing. Mol Ther (2016) 0.84
The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells. Mol Ther (2016) 0.83
Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia. Sci Rep (2014) 0.82
Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex. RNA Biol (2015) 0.82
A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research. Arterioscler Thromb Vasc Biol (2016) 0.81
SAMHD1 controls cell cycle status, apoptosis and HIV-1 infection in monocytic THP-1 cells. Virology (2016) 0.81
Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineering. Mol Ther Nucleic Acids (2015) 0.80
Genetic Basis of Melanin Pigmentation in Butterfly Wings. Genetics (2017) 0.80
In Vivo Zinc Finger Nuclease-mediated Targeted Integration of a Glucose-6-phosphatase Transgene Promotes Survival in Mice With Glycogen Storage Disease Type IA. Mol Ther (2016) 0.79
CPP-ZFN: a potential DNA-targeting anti-malarial drug. Malar J (2010) 0.79
A TALE nuclease architecture for efficient genome editing. Nat Biotechnol (2010) 26.47
Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature (2005) 19.15
Genome editing with engineered zinc finger nucleases. Nat Rev Genet (2010) 18.88
An improved zinc-finger nuclease architecture for highly specific genome editing. Nat Biotechnol (2007) 17.80
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat Biotechnol (2008) 14.75
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat Biotechnol (2008) 14.20
Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol (2009) 12.40
Knockout rats via embryo microinjection of zinc-finger nucleases. Science (2009) 12.33
Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol (2011) 11.76
Targeted genome editing across species using ZFNs and TALENs. Science (2011) 9.84
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery. Nat Biotechnol (2007) 9.23
Distinct factors control histone variant H3.3 localization at specific genomic regions. Cell (2010) 8.79
Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV. N Engl J Med (2014) 7.64
Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A (2008) 6.59
Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations. Cell (2011) 6.56
Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo. Nat Biotechnol (2010) 6.55
An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nat Biotechnol (2011) 6.44
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures. Nat Methods (2010) 6.00
Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases. Proc Natl Acad Sci U S A (2007) 5.93
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells. Nature (2011) 4.92
In vivo genome editing restores haemostasis in a mouse model of haemophilia. Nature (2011) 4.81
Precise genome modification in the crop species Zea mays using zinc-finger nucleases. Nature (2009) 4.71
Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome. Genome Res (2010) 3.94
Transient cold shock enhances zinc-finger nuclease-mediated gene disruption. Nat Methods (2010) 3.59
PPARgamma knockdown by engineered transcription factors: exogenous PPARgamma2 but not PPARgamma1 reactivates adipogenesis. Genes Dev (2002) 3.17
Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells. Nat Cell Biol (2011) 2.79
Drug discovery with engineered zinc-finger proteins. Nat Rev Drug Discov (2003) 2.63
Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology. Nucleic Acids Res (2010) 2.61
TRF2 associates with DREF and directs promoter-selective gene expression in Drosophila. Nature (2002) 2.48
Targeted transgene integration in plant cells using designed zinc finger nucleases. Plant Mol Biol (2008) 2.40
Site-specific integration and tailoring of cassette design for sustainable gene transfer. Nat Methods (2011) 2.30
Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases. Biotechnol Bioeng (2010) 2.23
DNA ligase III promotes alternative nonhomologous end-joining during chromosomal translocation formation. PLoS Genet (2011) 2.22
Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme. Genome Res (2012) 2.02
Efficient targeted gene disruption in the soma and germ line of the frog Xenopus tropicalis using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A (2011) 1.90
Simultaneous zinc-finger nuclease editing of the HIV coreceptors ccr5 and cxcr4 protects CD4+ T cells from HIV-1 infection. Blood (2013) 1.80
Site-specific genome editing in Plasmodium falciparum using engineered zinc-finger nucleases. Nat Methods (2012) 1.79
Translating dosage compensation to trisomy 21. Nature (2013) 1.75
The epithelial cell-derived atopic dermatitis cytokine TSLP activates neurons to induce itch. Cell (2013) 1.71
An engineered zinc finger protein activator of the endogenous glial cell line-derived neurotrophic factor gene provides functional neuroprotection in a rat model of Parkinson's disease. J Neurosci (2010) 1.59
Targeted gene addition to human mesenchymal stromal cells as a cell-based plasma-soluble protein delivery platform. Cytotherapy (2010) 1.58
Engineering HIV-resistant human CD4+ T cells with CXCR4-specific zinc-finger nucleases. PLoS Pathog (2011) 1.50
Genomic editing of the HIV-1 coreceptor CCR5 in adult hematopoietic stem and progenitor cells using zinc finger nucleases. Mol Ther (2013) 1.49
Development of a single-chain, quasi-dimeric zinc-finger nuclease for the selective degradation of mutated human mitochondrial DNA. Nucleic Acids Res (2008) 1.48
BAK and BAX deletion using zinc-finger nucleases yields apoptosis-resistant CHO cells. Biotechnol Bioeng (2010) 1.46
In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration. Biotechnol Bioeng (2012) 1.45
Cancer translocations in human cells induced by zinc finger and TALE nucleases. Genome Res (2013) 1.44
Engineered zinc finger proteins for controlling stem cell fate. Stem Cells (2003) 1.36
Enhanced protein production by engineered zinc finger proteins. Biotechnol Bioeng (2007) 1.26
Derivation and characterization of a simian immunodeficiency virus SIVmac239 variant with tropism for CXCR4. J Virol (2009) 1.24
Toward eliminating HLA class I expression to generate universal cells from allogeneic donors. Blood (2013) 1.17
Efficient clinical scale gene modification via zinc finger nuclease-targeted disruption of the HIV co-receptor CCR5. Hum Gene Ther (2013) 1.14
Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies. Biotechnol Bioeng (2010) 1.12
Robust ZFN-mediated genome editing in adult hemophilic mice. Blood (2013) 1.11
Zinc-finger nuclease editing of human cxcr4 promotes HIV-1 CD4(+) T cell resistance and enrichment. Mol Ther (2012) 1.10
Repression of vascular endothelial growth factor A in glioblastoma cells using engineered zinc finger transcription factors. Cancer Res (2003) 1.09
NA-Seq: a discovery tool for the analysis of chromatin structure and dynamics during differentiation. Dev Cell (2009) 1.07
Trait stacking via targeted genome editing. Plant Biotechnol J (2013) 1.06
Designed transcription factors as tools for therapeutics and functional genomics. Biochem Pharmacol (2002) 1.06