Published in Nat Methods on August 07, 2011
RNA-guided human genome engineering via Cas9. Science (2013) 48.29
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol (2013) 18.01
FLASH assembly of TALENs for high-throughput genome editing. Nat Biotechnol (2012) 12.97
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol (2013) 11.35
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol (2013) 8.40
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol (2014) 6.85
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Genome Res (2013) 6.19
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity. Nucleic Acids Res (2013) 4.11
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat Biotechnol (2014) 3.72
Precision genome engineering with programmable DNA-nicking enzymes. Genome Res (2012) 3.05
A library of TAL effector nucleases spanning the human genome. Nat Biotechnol (2013) 2.87
Targeted chromosomal duplications and inversions in the human genome using zinc finger nucleases. Genome Res (2011) 2.85
Therapeutic genome editing: prospects and challenges. Nat Med (2015) 2.42
Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis. Proc Natl Acad Sci U S A (2014) 2.26
Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects. Nucleic Acids Res (2012) 2.26
Targeted gene correction minimally impacts whole-genome mutational load in human-disease-specific induced pluripotent stem cell clones. Cell Stem Cell (2014) 2.24
Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods (2014) 2.04
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors. Nat Biotechnol (2015) 2.03
Advances in targeted genome editing. Curr Opin Chem Biol (2012) 1.97
Quantitative analysis of TALE-DNA interactions suggests polarity effects. Nucleic Acids Res (2013) 1.88
TALEN-based gene correction for epidermolysis bullosa. Mol Ther (2013) 1.84
Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity. Nat Methods (2014) 1.66
Deployment of new biotechnologies in plant breeding. Nat Biotechnol (2012) 1.64
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Cell Rep (2014) 1.50
Zinc-finger-nucleases mediate specific and efficient excision of HIV-1 proviral DNA from infected and latently infected human T cells. Nucleic Acids Res (2013) 1.48
A cut above the rest: targeted genome editing technologies in human pluripotent stem cells. J Biol Chem (2013) 1.47
Advances in fluorescence labeling strategies for dynamic cellular imaging. Nat Chem Biol (2014) 1.47
Efficient correction of hemoglobinopathy-causing mutations by homologous recombination in integration-free patient iPSCs. Cell Res (2011) 1.47
In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites. Nucleic Acids Res (2013) 1.46
An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage. Nucleic Acids Res (2013) 1.45
Genome editing technologies: defining a path to clinic. Mol Ther (2015) 1.41
Genome-editing Technologies for Gene and Cell Therapy. Mol Ther (2016) 1.41
Making designer mutants in model organisms. Development (2014) 1.38
Genome editing of human pluripotent stem cells to generate human cellular disease models. Dis Model Mech (2013) 1.37
Precision editing of large animal genomes. Adv Genet (2012) 1.34
megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering. Nucleic Acids Res (2013) 1.30
Coupling endonucleases with DNA end-processing enzymes to drive gene disruption. Nat Methods (2012) 1.28
Barcoding cells using cell-surface programmable DNA-binding domains. Nat Methods (2013) 1.25
Target specificity of the CRISPR-Cas9 system. Quant Biol (2014) 1.23
Type II restriction endonucleases--a historical perspective and more. Nucleic Acids Res (2014) 1.22
Efficient methods for targeted mutagenesis in zebrafish using zinc-finger nucleases: data from targeting of nine genes using CompoZr or CoDA ZFNs. PLoS One (2013) 1.19
TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity. Nucleic Acids Res (2014) 1.16
Zinc-finger nuclease-mediated correction of α-thalassemia in iPS cells. Blood (2012) 1.15
Quantifying on- and off-target genome editing. Trends Biotechnol (2015) 1.15
A TALE of two nucleases: gene targeting for the masses? Zebrafish (2011) 1.14
Expanding the genetic editing tool kit: ZFNs, TALENs, and CRISPR-Cas9. J Clin Invest (2014) 1.13
Targeting DNA With Fingers and TALENs. Mol Ther Nucleic Acids (2012) 1.12
TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS (MVA) syndrome. Proc Natl Acad Sci U S A (2013) 1.11
Hematopoietic stem cell engineering at a crossroads. Blood (2011) 1.10
Correction of dystrophin expression in cells from Duchenne muscular dystrophy patients through genomic excision of exon 51 by zinc finger nucleases. Mol Ther (2014) 1.09
Chimeric piggyBac transposases for genomic targeting in human cells. Nucleic Acids Res (2012) 1.09
Chromosomal context and epigenetic mechanisms control the efficacy of genome editing by rare-cutting designer endonucleases. Nucleic Acids Res (2012) 1.09
Highly significant antiviral activity of HIV-1 LTR-specific tre-recombinase in humanized mice. PLoS Pathog (2013) 1.08
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes. Cell (2016) 1.08
Zinc-finger nucleases: how to play two good hands. Nat Methods (2011) 1.08
Newer gene editing technologies toward HIV gene therapy. Viruses (2013) 1.07
Compact designer TALENs for efficient genome engineering. Nat Commun (2013) 1.06
AAV-mediated delivery of zinc finger nucleases targeting hepatitis B virus inhibits active replication. PLoS One (2014) 1.04
In vivo genome editing of the albumin locus as a platform for protein replacement therapy. Blood (2015) 1.03
Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases. Nat Rev Genet (2016) 1.03
Chromatin regulation at the frontier of synthetic biology. Nat Rev Genet (2015) 1.01
Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. Nucleic Acids Res (2013) 1.01
Synthetic epigenetics-towards intelligent control of epigenetic states and cell identity. Clin Epigenetics (2015) 0.99
High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs. Nucleic Acids Res (2014) 0.98
Seamless genome editing in human pluripotent stem cells using custom endonuclease-based gene targeting and the piggyBac transposon. Nat Protoc (2013) 0.97
Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9. Mol Cells (2015) 0.97
Implications of targeted genomic disruption of β-catenin in BxPC-3 pancreatic adenocarcinoma cells. PLoS One (2014) 0.96
Determining the specificities of TALENs, Cas9, and other genome-editing enzymes. Methods Enzymol (2014) 0.93
Generation and genetic engineering of human induced pluripotent stem cells using designed zinc finger nucleases. Stem Cells Dev (2012) 0.93
Find and replace: editing human genome in pluripotent stem cells. Protein Cell (2011) 0.93
A novel zinc-finger nuclease platform with a sequence-specific cleavage module. Nucleic Acids Res (2011) 0.92
A systematic survey of the Cys2His2 zinc finger DNA-binding landscape. Nucleic Acids Res (2015) 0.92
Targeted gene therapies: tools, applications, optimization. Crit Rev Biochem Mol Biol (2012) 0.91
Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity. Nat Biotechnol (2016) 0.90
Repeatable construction method for engineered zinc finger nuclease based on overlap extension PCR and TA-cloning. PLoS One (2013) 0.90
Assessing the risks of genotoxicity in the therapeutic development of induced pluripotent stem cells. Mol Ther (2012) 0.90
Progress and prospects in stem cell therapy. Acta Pharmacol Sin (2013) 0.90
Enabling functional genomics with genome engineering. Genome Res (2015) 0.90
EENdb: a database and knowledge base of ZFNs and TALENs for endonuclease engineering. Nucleic Acids Res (2012) 0.90
Genomic editing tools to model human diseases with isogenic pluripotent stem cells. Stem Cells Dev (2014) 0.89
Genome editing and the next generation of antiviral therapy. Hum Genet (2016) 0.88
Lens epithelium-derived growth factor/p75 qualifies as a target for HIV gene therapy in the NSG mouse model. Mol Ther (2012) 0.88
CCR5 Disruption in Induced Pluripotent Stem Cells Using CRISPR/Cas9 Provides Selective Resistance of Immune Cells to CCR5-tropic HIV-1 Virus. Mol Ther Nucleic Acids (2015) 0.87
Recent developments in human immunodeficiency virus-1 latency research. J Gen Virol (2013) 0.87
Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases. Nucleic Acids Res (2011) 0.87
Balancing between affinity and speed in target DNA search by zinc-finger proteins via modulation of dynamic conformational ensemble. Proc Natl Acad Sci U S A (2015) 0.87
Redesign of extensive protein-DNA interfaces of meganucleases using iterative cycles of in vitro compartmentalization. Proc Natl Acad Sci U S A (2014) 0.86
Sculpting genomes with a hammer and chisel. Nat Methods (2013) 0.86
Zinc fingers hit off target. Nat Med (2011) 0.86
Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases. PLoS One (2011) 0.85
On target? Tracing zinc-finger-nuclease specificity. Nat Methods (2011) 0.84
Synthetic biology in cell-based cancer immunotherapy. Trends Biotechnol (2015) 0.84
Targeted Gene Addition of Microdystrophin in Mice Skeletal Muscle via Human Myoblast Transplantation. Mol Ther Nucleic Acids (2013) 0.84
Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos. Proc Natl Acad Sci U S A (2012) 0.84
Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing. Mol Ther (2016) 0.84
Mapping the precision of genome editing. Nat Biotechnol (2015) 0.84
Site-specific Genome Editing in PBMCs With PLGA Nanoparticle-delivered PNAs Confers HIV-1 Resistance in Humanized Mice. Mol Ther Nucleic Acids (2013) 0.83
Gene correction in patient-specific iPSCs for therapy development and disease modeling. Hum Genet (2016) 0.83
Current progress and challenges in HIV gene therapy. Future Virol (2011) 0.82
Rosa26 locus supports tissue-specific promoter driving transgene expression specifically in pig. PLoS One (2014) 0.82
Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol (2000) 128.08
Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature (2005) 19.15
An improved zinc-finger nuclease architecture for highly specific genome editing. Nat Biotechnol (2007) 17.80
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat Biotechnol (2008) 14.75
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat Biotechnol (2008) 14.20
Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol (2009) 12.40
Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Proc Natl Acad Sci U S A (1996) 12.25
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell (2008) 11.24
Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics (2002) 9.09
Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Cell Stem Cell (2009) 8.97
Stimulation of homologous recombination through targeted cleavage by chimeric nucleases. Mol Cell Biol (2001) 6.68
Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A (2008) 6.59
Unexpected failure rates for modular assembly of engineered zinc fingers. Nat Methods (2008) 6.51
Targeted integration in rat and mouse embryos with zinc-finger nucleases. Nat Biotechnol (2010) 5.60
Exploring the DNA-binding specificities of zinc fingers with DNA microarrays. Proc Natl Acad Sci U S A (2001) 5.08
Toward controlling gene expression at will: selection and design of zinc finger domains recognizing each of the 5'-GNN-3' DNA target sequences. Proc Natl Acad Sci U S A (1999) 4.87
Efficient gene targeting in Drosophila with zinc-finger nucleases. Genetics (2006) 4.47
DNA-binding specificity is a major determinant of the activity and toxicity of zinc-finger nucleases. Mol Ther (2007) 3.94
Highly specific zinc finger proteins obtained by directed domain shuffling and cell-based selection. Proc Natl Acad Sci U S A (2003) 3.29
Analysis of zinc fingers optimized via phage display: evaluating the utility of a recognition code. J Mol Biol (1999) 3.20
Expanding or restricting the target site repertoire of zinc-finger nucleases: the inter-domain linker as a major determinant of target site selectivity. Mol Ther (2008) 3.02
FokI requires two specific DNA sites for cleavage. J Mol Biol (2001) 2.83
Evaluation of a modular strategy for the construction of novel polydactyl zinc finger DNA-binding proteins. Biochemistry (2003) 2.33
Bind-n-Seq: high-throughput analysis of in vitro protein-DNA interactions using massively parallel sequencing. Nucleic Acids Res (2009) 1.88
Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases. Nucleic Acids Res (2010) 1.81
Zinc-finger nucleases as a novel therapeutic strategy for targeting hepatitis B virus DNAs. Mol Ther (2010) 1.61
Frequent endonuclease cleavage at off-target locations in vivo. Mol Ther (2010) 1.52
Extensive protein and DNA backbone sampling improves structure-based specificity prediction for C2H2 zinc fingers. Nucleic Acids Res (2011) 1.27
Adding fingers to an engineered zinc finger nuclease can reduce activity. Biochemistry (2011) 1.17
Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Nucleic Acids Res (2007) 1.17
A novel Akt/PKB-interacting protein promotes cell adhesion and inhibits familial amyotrophic lateral sclerosis-linked mutant SOD1-induced neuronal death via inhibition of PP2A-mediated dephosphorylation of Akt/PKB. Cell Signal (2007) 1.09
Glucose metabolism-related protein 1 (GMRP1) regulates pancreatic beta cell proliferation and apoptosis via activation of Akt signalling pathway in rats and mice. Diabetologia (2011) 1.06
Molecular cloning and characterization of a novel human BTB domain-containing gene, BTBD10, which is down-regulated in glioma. Gene (2004) 1.05
Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science (2009) 32.97
Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol (2013) 21.32
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol (2013) 18.01
FLASH assembly of TALENs for high-throughput genome editing. Nat Biotechnol (2012) 12.97
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell (2008) 11.24
Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Cell Stem Cell (2009) 8.97
Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA). Nat Methods (2010) 8.86
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol (2013) 8.40
The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing. PLoS One (2010) 8.10
High-frequency modification of plant genes using engineered zinc-finger nucleases. Nature (2009) 7.19
Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol (2011) 7.05
Rapid mutation of endogenous zebrafish genes using zinc finger nucleases made by Oligomerized Pool ENgineering (OPEN). PLoS One (2009) 6.67
Unexpected failure rates for modular assembly of engineered zinc fingers. Nat Methods (2008) 6.51
Zinc-finger nucleases: the next generation emerges. Mol Ther (2008) 5.69
High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. Proc Natl Acad Sci U S A (2010) 4.78
CRISPR RNA-guided activation of endogenous human genes. Nat Methods (2013) 4.69
A small-molecule inhibitor of tgf-Beta signaling replaces sox2 in reprogramming by inducing nanog. Cell Stem Cell (2009) 4.64
Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Nat Protoc (2006) 4.16
DNA-binding specificity is a major determinant of the activity and toxicity of zinc-finger nucleases. Mol Ther (2007) 3.94
Locus-specific editing of histone modifications at endogenous enhancers. Nat Biotechnol (2013) 3.82
ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool. Nucleic Acids Res (2010) 3.59
Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool. Nucleic Acids Res (2007) 3.47
Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs. Nucleic Acids Res (2012) 3.41
Highly specific zinc finger proteins obtained by directed domain shuffling and cell-based selection. Proc Natl Acad Sci U S A (2003) 3.29
Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Nat Protoc (2009) 3.01
Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Nat Biotechnol (2013) 2.89
Robust, synergistic regulation of human gene expression using TALE activators. Nat Methods (2013) 2.89
Comparison of zinc finger nucleases for use in gene targeting in mammalian cells. Mol Ther (2008) 2.87
A system for the continuous directed evolution of biomolecules. Nature (2011) 2.77
Heritable and precise zebrafish genome editing using a CRISPR-Cas system. PLoS One (2013) 2.74
Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Nat Protoc (2009) 2.67
The pathogen-associated iroA gene cluster mediates bacterial evasion of lipocalin 2. Proc Natl Acad Sci U S A (2006) 2.63
Supercharging proteins can impart unusual resilience. J Am Chem Soc (2007) 2.52
In situ genetic correction of the sickle cell anemia mutation in human induced pluripotent stem cells using engineered zinc finger nucleases. Stem Cells (2011) 2.52
How pathogenic bacteria evade mammalian sabotage in the battle for iron. Nat Chem Biol (2006) 2.45
An affinity-based scoring scheme for predicting DNA-binding activities of modularly assembled zinc-finger proteins. Nucleic Acids Res (2008) 2.42
A synthetic biology framework for programming eukaryotic transcription functions. Cell (2012) 2.32
Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects. Nucleic Acids Res (2012) 2.26
Directed evolution and substrate specificity profile of homing endonuclease I-SceI. J Am Chem Soc (2006) 2.18
Opposing effects of Tcf3 and Tcf1 control Wnt stimulation of embryonic stem cell self-renewal. Nat Cell Biol (2011) 2.13
DNA-templated organic synthesis: nature's strategy for controlling chemical reactivity applied to synthetic molecules. Angew Chem Int Ed Engl (2004) 2.03
Reaction discovery enabled by DNA-templated synthesis and in vitro selection. Nature (2004) 1.99
Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins. Proc Natl Acad Sci U S A (2009) 1.95
DNA-templated organic synthesis and selection of a library of macrocycles. Science (2004) 1.95
Engineering a ligand-dependent RNA transcriptional activator. Chem Biol (2004) 1.94
Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion. Nucleic Acids Res (2010) 1.92
Zinc Finger Database (ZiFDB): a repository for information on C2H2 zinc fingers and engineered zinc-finger arrays. Nucleic Acids Res (2008) 1.87
Photoswitching mechanism of cyanine dyes. J Am Chem Soc (2009) 1.86
Improved somatic mutagenesis in zebrafish using transcription activator-like effector nucleases (TALENs). PLoS One (2012) 1.85
A general strategy for the evolution of bond-forming enzymes using yeast display. Proc Natl Acad Sci U S A (2011) 1.85
TALEN-based gene correction for epidermolysis bullosa. Mol Ther (2013) 1.84
Autonomous multistep organic synthesis in a single isothermal solution mediated by a DNA walker. Nat Nanotechnol (2010) 1.84
Discovery and biological characterization of geranylated RNA in bacteria. Nat Chem Biol (2012) 1.70
In vivo evolution of an RNA-based transcriptional activator. Chem Biol (2003) 1.69
Toddler: an embryonic signal that promotes cell movement via Apelin receptors. Science (2014) 1.66
A de novo protein binding pair by computational design and directed evolution. Mol Cell (2011) 1.65
Repression of phase-variable cup gene expression by H-NS-like proteins in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A (2005) 1.60
In vivo evolution of an RNA-based transcriptional silencing domain in S. cerevisiae. Chem Biol (2007) 1.56
Expanding the reaction scope of DNA-templated synthesis. Angew Chem Int Ed Engl (2002) 1.56
A biomolecule-compatible visible-light-induced azide reduction from a DNA-encoded reaction-discovery system. Nat Chem (2011) 1.54
In vitro characterization of IroB, a pathogen-associated C-glycosyltransferase. Proc Natl Acad Sci U S A (2004) 1.52
Sequence-controlled polymers. Science (2013) 1.50
High-throughput beta-galactosidase assay for bacterial cell-based reporter systems. Biotechniques (2004) 1.48
In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes. J Am Chem Soc (2005) 1.47
In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites. Nucleic Acids Res (2013) 1.46
Directed evolution of ligand dependence: small-molecule-activated protein splicing. Proc Natl Acad Sci U S A (2004) 1.46
Potent delivery of functional proteins into Mammalian cells in vitro and in vivo using a supercharged protein. ACS Chem Biol (2010) 1.42
Multistep small-molecule synthesis programmed by DNA templates. J Am Chem Soc (2002) 1.42
Enzyme-free translation of DNA into sequence-defined synthetic polymers structurally unrelated to nucleic acids. Nat Chem (2013) 1.41
Efficient and sequence-specific DNA-templated polymerization of peptide nucleic acid aldehydes. J Am Chem Soc (2003) 1.41
piggyBac transposase tools for genome engineering. Proc Natl Acad Sci U S A (2013) 1.35
Targeted mutagenesis of duplicated genes in soybean with zinc-finger nucleases. Plant Physiol (2011) 1.29
LC/MS analysis of cellular RNA reveals NAD-linked RNA. Nat Chem Biol (2009) 1.29
A protein interaction surface in nonribosomal peptide synthesis mapped by combinatorial mutagenesis and selection. Proc Natl Acad Sci U S A (2006) 1.28
Development and initial application of a hybridization-independent, DNA-encoded reaction discovery system compatible with organic solvents. J Am Chem Soc (2007) 1.25
Translation of DNA into a library of 13,000 synthetic small-molecule macrocycles suitable for in vitro selection. J Am Chem Soc (2008) 1.23
Nucleic acid evolution and minimization by nonhomologous random recombination. Nat Biotechnol (2002) 1.20
Small-molecule discovery from DNA-encoded chemical libraries. Chem Soc Rev (2011) 1.20
Engineering Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system. Methods Mol Biol (2007) 1.19
DNA-templated polymerization of side-chain-functionalized peptide nucleic acid aldehydes. J Am Chem Soc (2008) 1.18
Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Nucleic Acids Res (2007) 1.17
Translating the genomics revolution: the need for an international gene therapy consortium for monogenic diseases. Mol Ther (2013) 1.16
A class of human proteins that deliver functional proteins into mammalian cells in vitro and in vivo. Chem Biol (2011) 1.16
Highly sensitive in vitro selections for DNA-linked synthetic small molecules with protein binding affinity and specificity. J Am Chem Soc (2003) 1.15
Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes. Proc Natl Acad Sci U S A (2007) 1.15
Creating small-molecule-dependent switches to modulate biological functions. Chem Biol (2005) 1.14
A chemical screen for biological small molecule-RNA conjugates reveals CoA-linked RNA. Proc Natl Acad Sci U S A (2009) 1.12
Zinc-finger nucleases for somatic gene therapy: the next frontier. Hum Gene Ther (2011) 1.11
Engineering designer transcription activator-like effector nucleases (TALENs) by REAL or REAL-Fast assembly. Curr Protoc Mol Biol (2012) 1.10
Localized protein interaction surfaces on the EntB carrier protein revealed by combinatorial mutagenesis and selection. J Am Chem Soc (2006) 1.10
Evaluation of OPEN zinc finger nucleases for direct gene targeting of the ROSA26 locus in mouse embryos. PLoS One (2012) 1.10
Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution. Proc Natl Acad Sci U S A (2013) 1.09
Enzymatic tailoring of enterobactin alters membrane partitioning and iron acquisition. ACS Chem Biol (2006) 1.09
Directing otherwise incompatible reactions in a single solution by using DNA-templated organic synthesis. Angew Chem Int Ed Engl (2002) 1.08