PubRank

J J Holbrook

Author PubWeight™ 111.71‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Ethidium bromide as a cooperative effector of a DNA structure. Proc Natl Acad Sci U S A 1972 3.06
2 Equilibrium binding of nicotinamide nucleotides to lactate dehydrogenases. Biochem J 1973 2.77
3 A specific, highly active malate dehydrogenase by redesign of a lactate dehydrogenase framework. Science 1988 2.63
4 Ionic properties of an essential histidine residue in pig heart lactate dehydrogenase. Biochem J 1973 2.35
5 Protein fluorescence of lactate dehydrogenase. Biochem J 1972 2.21
6 Design and synthesis of new enzymes based on the lactate dehydrogenase framework. Philos Trans R Soc Lond B Biol Sci 1991 2.02
7 A single amino acid substitution in lactate dehydrogenase improves the catalytic efficiency with an alternative coenzyme. Biochem Biophys Res Commun 1990 1.84
8 Binding and hydrolysis of nucleotides in the chaperonin catalytic cycle: implications for the mechanism of assisted protein folding. Biochemistry 1993 1.78
9 Cloning, expression and complete nucleotide sequence of the Bacillus stearothermophilus L-lactate dehydrogenase gene. Gene 1986 1.76
10 The use of ternary complexes to study ionizations and isomerizations during catalysis by lactate dehydrogenase. Biochem J 1973 1.72
11 The identification of intermediates in the reaction of pig heart lactate dehydrogenase with its substrates. Biochem J 1974 1.71
12 Source of catalysis in the lactate dehydrogenase system. Ground-state interactions in the enzyme-substrate complex. Biochemistry 1994 1.64
13 Structure of a ternary complex of an allosteric lactate dehydrogenase from Bacillus stearothermophilus at 2.5 A resolution. J Mol Biol 1992 1.54
14 Reactivity of the essential thiol group of lactate dehydrogenase and substrate binding. Biochem J 1970 1.48
15 Site-directed mutagenesis reveals role of mobile arginine residue in lactate dehydrogenase catalysis. Nature 1987 1.43
16 A peptide containing a reactive lysyl group from ox liver glutamate dehydrogenase. Biochem J 1969 1.41
17 Protein fluorescence of nicotinamide nucleotide-dependent dehydrogenases. Biochem J 1972 1.40
18 Binding of a chaperonin to the folding intermediates of lactate dehydrogenase. Biochemistry 1991 1.38
19 An investigation of the contribution made by the carboxylate group of an active site histidine-aspartate couple to binding and catalysis in lactate dehydrogenase. Biochemistry 1988 1.25
20 The importance of SH-groups for enzymic activity. V. The coenzyme-binding capacity of pig heart lactate dehydrogenase, isozyme I, after inhibition by various maleinimides. Biochem Z 1966 1.22
21 The use of genetically engineered tryptophan to identify the movement of a domain of B. stearothermophilus lactate dehydrogenase with the process which limits the steady-state turnover of the enzyme. Biochem Biophys Res Commun 1988 1.18
22 The structure of lactate dehydrogenase from Plasmodium falciparum reveals a new target for anti-malarial design. Nat Struct Biol 1996 1.17
23 The importance of SH-groups for enzymic activity. 7. The amino acid sequence around the essential SH-group of pig heart lactate dehydrogenase, isoenzyme I. Eur J Biochem 1967 1.16
24 Malate dehydrogenase. X. Fluorescence microtitration studies of D-malate, hydroxymalonate, nicotinamide dinucleotide, and dihydronicotinamide-adenine dinucleotide binding by mitochondrial and supernatant porcine heart enzymes. Biochemistry 1972 1.16
25 The rates of defined changes in protein structure during the catalytic cycle of lactate dehydrogenase. Biochim Biophys Acta 1985 1.13
26 The multiple complexes formed by the interaction of platelet factor 4 with heparin. Biochem J 1980 1.13
27 The energetics and cooperativity of protein folding: a simple experimental analysis based upon the solvation of internal residues. Biochemistry 1993 1.08
28 NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase of Lactobacillus delbrueckii subsp. bulgaricus. Gene cloning and enzyme characterization. Eur J Biochem 1994 1.08
29 Calcium and the assays of human plasma clotting factor XIII. Biochem J 1974 1.06
30 From analysis to synthesis: new ligand binding sites on the lactate dehydrogenase framework. Part II. Trends Biochem Sci 1989 1.06
31 Slow structural changes shown by the 3-nitrotyrosine-237 residue in pig heart [Tyr(3NO2)237] lactate dehydrogenase. Biochem J 1982 1.06
32 A strong carboxylate-arginine interaction is important in substrate orientation and recognition in lactate dehydrogenase. Biochim Biophys Acta 1987 1.05
33 The amino acid sequence around the reactive cysteine residue in human plasma Factor XII. Biochem J 1973 1.03
34 Malate dehydrogenase of the cytosol. Ionizations of the enzyme-reduced-coenzyme complex and a comparison with lactate dehydrogenase. Biochem J 1978 1.03
35 From analysis to synthesis: new ligand binding sites on the lactate dehydrogenase framework. Part I. Trends Biochem Sci 1989 1.03
36 The stability and hydrophobicity of cytosolic and mitochondrial malate dehydrogenases and their relation to chaperonin-assisted folding. FEBS Lett 1994 1.01
37 Designs for a broad substrate specificity keto acid dehydrogenase. Biochemistry 1990 1.00
38 [Significance of SH groups for enzymatic activity. 3. A method for the radioactive marking of the essential cystein residue in native lactate dehydrogenase (isozyme I) in pig heart]. Biochem Z 1965 0.99
39 The site at which 4-iodoacetamidosalicylate reacts with glutamate dehydrogenases. Biochem J 1973 0.98
40 D175 discriminates between NADH and NADPH in the coenzyme binding site of Lactobacillus delbrueckii subsp. bulgaricus D-lactate dehydrogenase. Biochem Biophys Res Commun 1995 0.98
41 The importance of arginine 102 for the substrate specificity of Escherichia coli malate dehydrogenase. Biochem Biophys Res Commun 1992 0.98
42 The calcium-induced dissociation of human plasma clotting factor XIII. Biochem J 1974 0.98
43 Detection and characterization of intermediates in the folding of large proteins by the use of genetically inserted tryptophan probes. Biochemistry 1991 0.97
44 The effect of modifying lysine-126 on the physical, catalytic and regulatory properties of bovine liver glutamate dehydrogenase. Biochem J 1973 0.95
45 Removal of substrate inhibition in a lactate dehydrogenase from human muscle by a single residue change. FEBS Lett 1996 0.94
46 13C-NMR and transient kinetic studies on lactate dehydrogenase [Cys(13CN)165]. Direct measurement of a rate-limiting rearrangement in protein structure. Biochim Biophys Acta 1986 0.93
47 Use of the sulphite adduct of nicotinamide-adenine dinucleotide to study ionizations and the kinetics of lactate dehydrogenase and malate dehydrogenase. Biochem J 1978 0.93
48 An equilibrium study of metal ion binding to human plasma coagulation factor XIII. Biochem J 1978 0.93
49 Isolation, sequence and overexpression of the gene encoding NAD-dependent formate dehydrogenase from the methylotrophic yeast Candida methylica. Gene 1995 0.93
50 Thermal motion as the rate-limiting step in the superprecipitation of actomyosin gels. Arch Biochem Biophys 1967 0.93
51 Differences in the protein fluorecence of the two iron(III)-binding sites of ovotransferrin. Biochem J 1975 0.93
52 Protein-protein interactions in blood clotting. The use of polarization of fluorescence to measure the dissociation of plasma factor XIIIa. Biochem J 1978 0.92
53 Engineering surface loops of proteins--a preferred strategy for obtaining new enzyme function. Trends Biotechnol 1994 0.91
54 [Enzymatic catalysis of cyanide addition to nicotinamide-adenin-nucleotide]. Biochem Z 1965 0.91
55 The oxaloacetate reductase activity of vertebrate lactate dehydrogenase. Int J Biochem 1981 0.90
56 A single amino acid substitution deregulates a bacterial lactate dehydrogenase and stabilizes its tetrameric structure. Biochim Biophys Acta 1987 0.90
57 Calcium and thiol reactivity of human plasma clotting factor XIII. Biochem J 1974 0.90
58 Charge balance in the alpha-hydroxyacid dehydrogenase vacuole: an acid test. Protein Sci 1992 0.90
59 Histidine residues and the enzyme activity of pig heart supernatant malate dehydrogenase. Biochem J 1974 0.89
60 Malate dehydrogenase of the cytosol. Preparation and reduced nicotinamide-adenine dinucleotide-binding studies. Biochem J 1978 0.89
61 The A245K mutation exposes another stage of the bacterial L-lactate dehydrogenase reaction mechanism. Biochemistry 2001 0.88
62 Guided evolution of enzymes with new substrate specificities. J Mol Biol 1996 0.87
63 The importance of arginine 171 in substrate binding by Bacillus stearothermophilus lactate dehydrogenase. Biochem Biophys Res Commun 1987 0.86
64 Mapping motion in large proteins by single tryptophan probes inserted by site-directed mutagenesis: lactate dehydrogenase. Biochem Soc Trans 1987 0.86
65 Amino acid sequence of the L-lactate dehydrogenase of Bacillus caldotenax deduced from the nucleotide sequence of the cloned gene. Eur J Biochem 1987 0.85
66 The effect of the chain length of heparin on its interaction with lipoprotein lipase. Biochim Biophys Acta 1983 0.85
67 The reaction of a histidine residue in glutamate dehydrogenase with diethyl pyrocarbonate. Biochem J 1973 0.85
68 The engineering of a more thermally stable lactate dehydrogenase by reduction of the area of a water-accessible hydrophobic surface. Biochim Biophys Acta 1987 0.84
69 The greater strength of arginine: carboxylate over lysine carboxylate ion pairs implications for the design of novel enzymes and drugs. Biochem Biophys Res Commun 1987 0.83
70 A model of Plasmodium falciparum lactate dehydrogenase and its implications for the design of improved antimalarials and the enhanced detection of parasitaemia. Protein Eng 1997 0.83
71 Kinetic analysis of experiments involving the single turnover of an enzyme. Biochem J 1976 0.83
72 A general method for relieving substrate inhibition in lactate dehydrogenases. Protein Eng 1999 0.83
73 Crystallization of a ternary complex of lactate dehydrogenase from Bacillus stearothermophilus. J Mol Biol 1988 0.83
74 The mechanism of activation of lipoprotein lipase by apolipoprotein C-II. The formation of a protein-protein complex in free solution and at a triacylglycerol/water interface. Biochim Biophys Acta 1985 0.83
75 H NMR studies of Tyr-237 of lactate dehydrogenase. FEBS Lett 1981 0.83
76 Allosteric activation in Bacillus stearothermophilus lactate dehydrogenase investigated by an X-ray crystallographic analysis of a mutant designed to prevent tetramerization of the enzyme. J Mol Biol 1994 0.83
77 The transfer of platelet factor 4 from its proteoglycan carrier to natural and synthetic polymers. Biochim Biophys Acta 1981 0.83
78 Low-resolution structure of the complex of human blood platelet factor 4 with heparin determined by small-angle neutron scattering. Biochim Biophys Acta 1986 0.82
79 D-2-hydroxy-4-methylvalerate dehydrogenase from Lactobacillus delbrueckii subsp. bulgaricus. II. Mutagenic analysis of catalytically important residues. Eur J Biochem 1997 0.82
80 Contribution of a buried aspartate residue towards the catalytic efficiency and structural stability of Bacillus stearothermophilus lactate dehydrogenase. Biochem J 1994 0.81
81 NADH binding to porcine mitochondrial malate dehydrogenase. J Biol Chem 1979 0.81
82 Dissecting the contributions of a specific side-chain interaction to folding and catalysis of Bacillus stearothermophilus lactate dehydrogenase. Eur J Biochem 1993 0.80
83 Changes in the state of subunit association of lactate dehydrogenase from Bacillus stearothermophilus. Biochim Biophys Acta 1985 0.80
84 Multiple complexes of thrombin and heparin. Biochim Biophys Acta 1986 0.80
85 Direct measurement of proton binding to the active ternary complex of pig heart lactate dehydrogenase. Biochem J 1973 0.80
86 The putative isomerization of the lactate dehydrogenase--NADH complex. FEBS Lett 1977 0.79
87 The catalysis by heparin of the reaction between thrombin and antithrombin. Biochim Biophys Acta 1986 0.79
88 Studies on the changes in protein fluorescence and enzymic activity of aspartate aminotransferase on binding of pyridoxal 5'-phosphate. Biochem J 1974 0.79
89 Duck liver 'malic' enzyme. Expression in Escherichia coli and characterization of the wild-type enzyme and site-directed mutants. Biochem J 1992 0.78
90 The influence of chaperonins on protein folding. A mechanism for increasing the yield of the native form. Ann N Y Acad Sci 1992 0.78
91 Opportunities and limits in creating new enzymes. Experiences with the NAD-dependent lactate dehydrogenase frameworks of humans and bacteria. Ann N Y Acad Sci 1992 0.78
92 Towards the construction of a universal NAD(P)(+)-dependent dehydrogenase: comparative and evolutionary considerations. Biochem Soc Trans 1991 0.78
93 The structural consequences of exchanging tryptophan and tyrosine residues in B. stearothermophilus lactate dehydrogenase. Protein Eng 1992 0.78
94 A prediction of the three-dimensional structure of maize NADP(+)-dependent malate dehydrogenase which explains aspects of light-dependent regulation unique to plant enzymes. J Comput Aided Mol Des 1992 0.78
95 Alteration of enzyme specificity and catalysis by protein engineering. Curr Opin Biotechnol 1991 0.77
96 D-2-hydroxy-4-methylvalerate dehydrogenase from Lactobacillus delbrueckii subsp. bulgaricus. I. Kinetic mechanism and pH dependence of kinetic parameters, coenzyme binding and substrate inhibition. Eur J Biochem 1997 0.77
97 Design of a specific phenyllactate dehydrogenase by peptide loop exchange on the Bacillus stearothermophilus lactate dehydrogenase framework. Biochemistry 1992 0.77
98 The use of site-directed mutagenesis and time-resolved fluorescence spectroscopy to assign the fluorescence contributions of individual tryptophan residues in Bacillus stearothermophilus lactate dehydrogenase. Biochim Biophys Acta 1987 0.77
99 Expression of the copy DNA for human A4 and B4 L-lactate dehydrogenases in Escherichia coli. Biochim Biophys Acta 1990 0.77
100 On the effect on specificity of Thr246----Gly mutation in L-lactate dehydrogenase of Bacillus sterothermophilus. Biochem Biophys Res Commun 1989 0.77
101 pH-dependent changes of intrinsic fluorescence of chemically modified liver alcohol dehydrogenases. Biochem J 1978 0.76
102 Production of an activated form of Bacillus stearothermophilus L-2-hydroxyacid dehydrogenase by directed evolution. Protein Eng 2000 0.76
103 Cancer-associated lactate dehydrogenase is a tyrosylphosphorylated form of human LDH-A, skeletal muscle isoenzyme. Cancer Invest 1988 0.75
104 Studies on the glutamate dehydrogenase from Neurospora crassa. Biochem J 1972 0.75
105 A study of the interaction between bovine cardiac-muscle cyclic AMP-dependent protein kinase and cyclic AMP using fluorescence-polarization spectroscopy. Biochem J 1984 0.75
106 Coenzyme binding at different ionization states of cytoplasmic and mitochondrial malate dehydrogenase. Z Naturforsch C 1982 0.75
107 The interaction of platelet factor 4 with heparins of different chain length. Biochim Biophys Acta 1984 0.75
108 Cloning, sequencing and functional expression of a DNA encoding pig cytosolic malate dehydrogenase: purification and characterization of the recombinant enzyme. Gene 1996 0.75
109 Substitution of the amino acid at position 102 with polar and aromatic residues influences substrate specificity of lactate dehydrogenase. J Protein Chem 1994 0.75
110 Correlation of the enzyme activities of Bacillus stearothermophilus lactate dehydrogenase on three substrates with the results of molecular dynamics/energy minimization conformational searching. Proteins 1997 0.75
111 The effect of concentration and ionic strength on the aggregation of beta-thromboglobulin. Thromb Res 1982 0.75
112 Factor XIII: a study using polarization of fluorescence of the activation of the enzyme by calcium and by substrate [proceedings]. Biochem Soc Trans 1977 0.75
113 Preparation of regulatory subunits from bovine heart cAMP-dependent protein kinase by a nondenaturing method. Methods Enzymol 1988 0.75
114 The preparation of cyclic nucleotide-depleted regulatory subunits from type II adenosine 3':5'-monophosphate-dependent protein kinase by a nondenaturing method. Anal Biochem 1984 0.75
115 A general method of domain closure is applied to phosphoglycerate kinase and the result compared with the crystal structure of a closed conformation of the enzyme. Proteins 1998 0.75
116 Protein engineering tests of a homology model of Plasmodium falciparum lactate dehydrogenase. Protein Eng 1997 0.75
117 The rational construction of new enzymes: design and synthesis of a dehydrogenase which accepts an unnatural substrate. Sci Prog 1989 0.75
118 Calcium and the assembly of human blood-clotting factor XIII. Biochem Soc Trans 1979 0.75
119 Multivalent proteins and multidentate polysaccharides: interaction of platelet factor 4 and heparin. Biochem Soc Trans 1981 0.75