Published in Science on April 06, 1990
Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. Proc Natl Acad Sci U S A (2005) 12.21
Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PLoS Biol (2004) 10.88
Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Science (2008) 10.19
In vivo two-photon calcium imaging of neuronal networks. Proc Natl Acad Sci U S A (2003) 9.97
Structural and molecular interrogation of intact biological systems. Nature (2013) 8.95
Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proc Natl Acad Sci U S A (2003) 8.92
ScanImage: flexible software for operating laser scanning microscopes. Biomed Eng Online (2003) 8.59
Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proc Natl Acad Sci U S A (2000) 8.47
Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex. Proc Natl Acad Sci U S A (1998) 6.95
Analysis of dynamic brain imaging data. Biophys J (1999) 6.81
Collagen reorganization at the tumor-stromal interface facilitates local invasion. BMC Med (2006) 6.44
Imaging large-scale neural activity with cellular resolution in awake, mobile mice. Neuron (2007) 6.38
Microscopy and its focal switch. Nat Methods (2009) 6.37
Probing the limits to positional information. Cell (2007) 6.23
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. Proc Natl Acad Sci U S A (2005) 5.96
Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy. Proc Natl Acad Sci U S A (1996) 5.78
Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues. Biophys J (2002) 5.70
Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nat Protoc (2009) 5.68
Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment. Biophys J (1995) 5.66
Going deeper than microscopy: the optical imaging frontier in biology. Nat Methods (2010) 5.65
Stability and nuclear dynamics of the bicoid morphogen gradient. Cell (2007) 5.31
Stably maintained dendritic spines are associated with lifelong memories. Nature (2009) 5.16
Functional specificity of local synaptic connections in neocortical networks. Nature (2011) 5.07
Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. Biophys J (1999) 4.97
Interpreting second-harmonic generation images of collagen I fibrils. Biophys J (2004) 4.74
Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence. Proc Natl Acad Sci U S A (2002) 4.73
Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy. Proc Natl Acad Sci U S A (1998) 4.72
In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia. Proc Natl Acad Sci U S A (2007) 4.53
Locally dynamic synaptic learning rules in pyramidal neuron dendrites. Nature (2007) 4.48
Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys J (1998) 4.44
Autonomous T cell trafficking examined in vivo with intravital two-photon microscopy. Proc Natl Acad Sci U S A (2003) 4.22
Rapid redistribution of synaptic PSD-95 in the neocortex in vivo. PLoS Biol (2006) 4.12
Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein. Biophys J (2002) 4.10
Detection of functional haematopoietic stem cell niche using real-time imaging. Nature (2008) 4.06
Two-photon tissue imaging: seeing the immune system in a fresh light. Nat Rev Immunol (2002) 4.01
Fluorescence lifetime measurements and biological imaging. Chem Rev (2010) 3.99
Fluorescence correlation spectroscopy: diagnostics for sparse molecules. Proc Natl Acad Sci U S A (1997) 3.90
Functional imaging of hippocampal place cells at cellular resolution during virtual navigation. Nat Neurosci (2010) 3.88
Two-photon imaging of cortical surface microvessels reveals a robust redistribution in blood flow after vascular occlusion. PLoS Biol (2006) 3.77
Coherent anti-stokes Raman scattering imaging of axonal myelin in live spinal tissues. Biophys J (2005) 3.62
In vivo photoacoustic tomography of chemicals: high-resolution functional and molecular optical imaging at new depths. Chem Rev (2010) 3.55
Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganization. Biophys J (2008) 3.49
Diffusion and scaling during early embryonic pattern formation. Proc Natl Acad Sci U S A (2005) 3.46
Photobleaching in two-photon excitation microscopy. Biophys J (2000) 3.46
Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability. Nat Biotechnol (1999) 3.44
Highly nonlinear photodamage in two-photon fluorescence microscopy. Biophys J (2001) 3.34
Estimating intracellular calcium concentrations and buffering without wavelength ratioing. Biophys J (2000) 3.25
Caged compounds: photorelease technology for control of cellular chemistry and physiology. Nat Methods (2007) 3.22
Three-dimensional morphology and gene expression in the Drosophila blastoderm at cellular resolution I: data acquisition pipeline. Genome Biol (2006) 3.22
Dynamic in vivo imaging and cell tracking using a histone fluorescent protein fusion in mice. BMC Biotechnol (2004) 3.20
Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior. Nat Methods (2010) 3.17
Brain-wide neuronal dynamics during motor adaptation in zebrafish. Nature (2012) 3.17
A functional link between dynamin and the actin cytoskeleton at podosomes. J Cell Biol (2000) 3.12
Thinned-skull cranial window technique for long-term imaging of the cortex in live mice. Nat Protoc (2010) 3.11
In vivo performance of genetically encoded indicators of neural activity in flies. J Neurosci (2005) 3.11
Serial two-photon tomography for automated ex vivo mouse brain imaging. Nat Methods (2012) 3.09
Macromolecular-scale resolution in biological fluorescence microscopy. Proc Natl Acad Sci U S A (2006) 3.04
Scanning two-photon fluctuation correlation spectroscopy: particle counting measurements for detection of molecular aggregation. Biophys J (1996) 3.04
Probing protein oligomerization in living cells with fluorescence fluctuation spectroscopy. Proc Natl Acad Sci U S A (2003) 3.02
SLM Microscopy: Scanless Two-Photon Imaging and Photostimulation with Spatial Light Modulators. Front Neural Circuits (2008) 3.01
Central dogma at the single-molecule level in living cells. Nature (2011) 3.00
Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Arch Toxicol (2013) 3.00
Open source bioimage informatics for cell biology. Trends Cell Biol (2009) 2.93
Electrophysiology in the age of light. Nature (2009) 2.90
Advances in light microscopy for neuroscience. Annu Rev Neurosci (2009) 2.88
Optical brain imaging in vivo: techniques and applications from animal to man. J Biomed Opt (2007) 2.87
Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing. Proc Natl Acad Sci U S A (2006) 2.85
Ca2+ fluorescence imaging with pico- and femtosecond two-photon excitation: signal and photodamage. Biophys J (1999) 2.83
In vivo three-photon microscopy of subcortical structures within an intact mouse brain. Nat Photonics (2013) 2.79
High-resolution nonlinear optical imaging of live cells by second harmonic generation. Biophys J (1999) 2.74
Nanotools for neuroscience and brain activity mapping. ACS Nano (2013) 2.74
Imaging chromophores with undetectable fluorescence by stimulated emission microscopy. Nature (2009) 2.74
Deep-tissue focal fluorescence imaging with digitally time-reversed ultrasound-encoded light. Nat Commun (2012) 2.73
Control of visually guided behavior by distinct populations of spinal projection neurons. Nat Neurosci (2008) 2.72
Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum. Nat Cell Biol (2003) 2.62
Dynamic remodeling of dendritic arbors in GABAergic interneurons of adult visual cortex. PLoS Biol (2005) 2.61
Imaging voltage in neurons. Neuron (2011) 2.54
Live-cell imaging of dendritic spines by STED microscopy. Proc Natl Acad Sci U S A (2008) 2.53
The coding of temperature in the Drosophila brain. Cell (2011) 2.53
A directional tuning map of Drosophila elementary motion detectors. Nature (2013) 2.53
Interferometric synthetic aperture microscopy. Nat Phys (2007) 2.52
Photoremovable protecting groups in chemistry and biology: reaction mechanisms and efficacy. Chem Rev (2012) 2.49
Structure and emergence of specific olfactory glomeruli in the mouse. J Neurosci (2001) 2.40
Multilayer three-dimensional super resolution imaging of thick biological samples. Proc Natl Acad Sci U S A (2008) 2.37
Two-photon high-resolution measurement of partial pressure of oxygen in cerebral vasculature and tissue. Nat Methods (2010) 2.37
Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell. Proc Natl Acad Sci U S A (2008) 2.36
Sentinel lymph nodes in the rat: noninvasive photoacoustic and US imaging with a clinical US system. Radiology (2010) 2.35
Brominated 7-hydroxycoumarin-4-ylmethyls: photolabile protecting groups with biologically useful cross-sections for two photon photolysis. Proc Natl Acad Sci U S A (1999) 2.33
Adaptive aberration correction in a confocal microscope. Proc Natl Acad Sci U S A (2002) 2.32
Calcium dynamics associated with action potentials in single nerve terminals of pyramidal cells in layer 2/3 of the young rat neocortex. J Physiol (2000) 2.30
In vivo molecular photoacoustic tomography of melanomas targeted by bioconjugated gold nanocages. ACS Nano (2010) 2.29
Multiphoton microscopy of endogenous fluorescence differentiates normal, precancerous, and cancerous squamous epithelial tissues. Cancer Res (2005) 2.28
Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology. Biophys J (2002) 2.26
Alternatives to gadolinium-based metal chelates for magnetic resonance imaging. Chem Rev (2010) 2.25
Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye. J Cell Biol (2004) 2.24
Three-dimensional micropatterning of bioactive hydrogels via two-photon laser scanning photolithography for guided 3D cell migration. Biomaterials (2008) 2.23
Helicobacter pylori immune escape is mediated by dendritic cell-induced Treg skewing and Th17 suppression in mice. Gastroenterology (2009) 2.22
Coherent nonlinear optical imaging: beyond fluorescence microscopy. Annu Rev Phys Chem (2011) 2.22
Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control. PLoS Biol (2004) 2.20
Opposite effects of fear conditioning and extinction on dendritic spine remodelling. Nature (2012) 2.19
Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys J (1976) 25.12
Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers (1974) 17.35
Dynamics of fluorescence marker concentration as a probe of mobility. Biophys J (1976) 9.67
Zero-mode waveguides for single-molecule analysis at high concentrations. Science (2003) 7.69
Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts. Proc Natl Acad Sci U S A (1976) 6.27
Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy. Proc Natl Acad Sci U S A (1996) 5.78
Constrained diffusion or immobile fraction on cell surfaces: a new interpretation. Biophys J (1996) 5.78
Automated detection and tracking of individual and clustered cell surface low density lipoprotein receptor molecules. Biophys J (1994) 5.61
Concentration correlation spectroscopy: a new biophysical probe based on occupation number fluctuations. Annu Rev Biophys Bioeng (1975) 5.56
Characterization of lipid bilayer phases by confocal microscopy and fluorescence correlation spectroscopy. Proc Natl Acad Sci U S A (1999) 5.19
Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes. Cytometry (1999) 4.98
Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. Biophys J (1999) 4.97
Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers. Proc Natl Acad Sci U S A (1976) 4.86
Fluorescence staining of the actin cytoskeleton in living cells with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin. Proc Natl Acad Sci U S A (1980) 4.77
Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy. Proc Natl Acad Sci U S A (1998) 4.72
Fluorescence correlation spectroscopy: diagnostics for sparse molecules. Proc Natl Acad Sci U S A (1997) 3.90
Two-photon molecular excitation provides intrinsic 3-dimensional resolution for laser-based microscopy and microphotochemistry. FASEB J (1994) 3.70
Measuring serotonin distribution in live cells with three-photon excitation. Science (1997) 3.59
Lateral transport of a lipid probe and labeled proteins on a cell membrane. Science (1977) 3.50
Fluorescent low density lipoprotein for observation of dynamics of individual receptor complexes on cultured human fibroblasts. J Cell Biol (1981) 3.29
Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins. Proc Natl Acad Sci U S A (2000) 3.29
Design of organic molecules with large two-photon absorption cross sections. Science (1998) 3.27
Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery. Biophys J (1999) 3.24
Diffusion of low density lipoprotein-receptor complex on human fibroblasts. J Cell Biol (1982) 3.16
Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine). Proc Natl Acad Sci U S A (2000) 3.07
Exchange of protein molecules through connections between higher plant plastids. Science (1997) 2.85
Optical imaging of cell membrane potential changes induced by applied electric fields. Biophys J (1986) 2.82
Lateral diffusion in planar lipid bilayers. Science (1977) 2.82
Lateral diffusion in phospholipid bilayer membranes and multilamellar liquid crystals. Biochemistry (1978) 2.82
Lateral motion and valence of Fc receptors on rat peritoneal mast cells. Nature (1976) 2.69
Molecular mobility on the cell surface. Biochem Soc Symp (1981) 2.59
Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints. J Cell Biol (1982) 2.52
Molecular crowding on the cell surface. Science (1988) 2.47
Mechanical properties of sensory hair bundles are reflected in their Brownian motion measured with a laser differential interferometer. Proc Natl Acad Sci U S A (1989) 2.38
Molecular counting of low-density lipoprotein particles as individuals and small clusters on cell surfaces. Biophys J (1986) 2.37
Two-Photon Fluorescence Excitation Cross Sections of Biomolecular Probes from 690 to 960 nm. Appl Opt (1998) 2.31
Thermal fluctuations of large cylindrical phospholipid vesicles. Biophys J (1984) 2.28
Multiphoton microscopy in biological research. Curr Opin Chem Biol (2001) 2.27
Three-dimensionally resolved NAD(P)H cellular metabolic redox imaging of the in situ cornea with two-photon excitation laser scanning microscopy. J Microsc (1995) 2.26
Growth arrest of individual senile plaques in a model of Alzheimer's disease observed by in vivo multiphoton microscopy. J Neurosci (2001) 2.12
Large-scale co-aggregation of fluorescent lipid probes with cell surface proteins. J Cell Biol (1994) 2.11
Membrane elasticity in giant vesicles with fluid phase coexistence. Biophys J (2005) 1.97
Active protein transport through plastid tubules: velocity quantified by fluorescence correlation spectroscopy. J Cell Sci (2000) 1.96
Lateral diffusion of gramicidin C in phospholipid multibilayers. Effects of cholesterol and high gramicidin concentration. Biophys J (1982) 1.88
Forward and reverse transduction at the limit of sensitivity studied by correlating electrical and mechanical fluctuations in frog saccular hair cells. Hear Res (1992) 1.86
Multiphoton-excited visible emission by serotonin solutions. Photochem Photobiol (1997) 1.85
Lipid diffusibility in the intact erythrocyte membrane. Biophys J (1983) 1.82
Receptor diffusion on cell surfaces modulated by locally bound concanavalin A. Proc Natl Acad Sci U S A (1977) 1.79
Fast diffusion along defects and corrugations in phospholipid P beta, liquid crystals. Biophys J (1983) 1.78
Isolated-patch recording from liposomes containing functionally reconstituted chloride channels from Torpedo electroplax. Proc Natl Acad Sci U S A (1982) 1.77
Quantitative comparison of background rejection, signal-to-noise ratio, and resolution in confocal and full-field laser scanning microscopes. Appl Opt (1995) 1.75
Fluorescence correlation spectroscopy: inception, biophysical experimentations, and prospectus. Appl Opt (2001) 1.73
Fluorescence studies on modes of cytochalasin B and phallotoxin action on cytoplasmic streaming in Chara. J Cell Biol (1981) 1.67
Lipid-glass adhesion in giga-sealed patch-clamped membranes. Biophys J (1994) 1.65
Single-molecule detection by two-photon-excited fluorescence. Opt Lett (1995) 1.64
Synthesis, photochemistry, and biological activity of a caged photolabile acetylcholine receptor ligand. Biochemistry (1989) 1.64
Cross-linking of receptor-bound IgE to aggregates larger than dimers leads to rapid immobilization. J Cell Biol (1986) 1.61
Single granule pH cycling in antigen-induced mast cell secretion. J Cell Sci (2000) 1.59
Transduction of membrane tension by the ion channel alamethicin. Biophys J (1994) 1.57
Delivery of nanojoule femtosecond pulses through large-core microstructured fibers. Opt Lett (2002) 1.55
The green fluorescent protein as a marker to visualize plant mitochondria in vivo. Plant J (1997) 1.53
Mechanism of the membrane potential sensitivity of the fluorescent membrane probe merocyanine 540. Biochemistry (1978) 1.52
Diffusion and patching of macromolecules on planar lipid bilayer membranes. Biochemistry (1977) 1.51
Unconstrained lateral diffusion of concanavalin A receptors on bulbous lymphocytes. Proc Natl Acad Sci U S A (1982) 1.51
Background rejection and signal-to-noise optimization in confocal and alternative fluorescence microscopes. Appl Opt (1994) 1.46
Mechanical response of frog saccular hair bundles to the aminoglycoside block of mechanoelectrical transduction. J Neurophysiol (1992) 1.45
Optical measurement of picometer displacements of transparent microscopic objects. Appl Opt (1990) 1.45
Photolysis of caged calcium in femtoliter volumes using two-photon excitation. Biophys J (1999) 1.42
Mobility and distribution of a cell surface glycoprotein and its interaction with other membrane components. Proc Natl Acad Sci U S A (1977) 1.41
F-actin aggregates in transformed cells. J Cell Biol (1981) 1.40
Clustering, mobility, and triggering activity of small oligomers of immunoglobulin E on rat basophilic leukemia cells. J Cell Biol (1986) 1.40
Two classes of alamethicin transmembrane channels: molecular models from single-channel properties. Biophys J (1995) 1.35
Overcoming photodamage in second-harmonic generation microscopy: real-time optical recording of neuronal action potentials. Proc Natl Acad Sci U S A (2006) 1.32
Mucosal mast cell secretion processes imaged using three-photon microscopy of 5-hydroxytryptamine autofluorescence. Biophys J (1999) 1.32
In vivo staining of cytoskeletal actin by autointernalization of nontoxic concentrations of nitrobenzoxadiazole-phallacidin. J Cell Biol (1981) 1.31
Three-dimensional optical data storage in refractive media by two-photon point excitation. Opt Lett (1991) 1.30
Applications of fluorescence correlation spectroscopy. Q Rev Biophys (1976) 1.30
Immunoglobulin E receptor cross-linking induces oscillations in intracellular free ionized calcium in individual tumor mast cells. J Biol Chem (1989) 1.29
Imaging [Ca2+]i dynamics during signal transduction. Cell Calcium (1990) 1.28
Imaging asynchronous changes in intracellular Ca2+ in individual stimulated tumor mast cells. Proc Natl Acad Sci U S A (1988) 1.28
F-actin in conifer roots. Proc Natl Acad Sci U S A (1982) 1.27
Differential staining of actin in metaphase spindles with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin and fluorescent DNase: is actin involved in chromosomal movement? Proc Natl Acad Sci U S A (1981) 1.21
Diffusion, patching, and capping of stearoylated dextrans on 3T3 cell plasma membranes. Biochemistry (1980) 1.21
Effects of protein concentration on IgE receptor mobility in rat basophilic leukemia cell plasma membranes. Biophys J (1992) 1.20
Creating biological membranes on the micron scale: forming patterned lipid bilayers using a polymer lift-off technique. Biophys J (2003) 1.17
Two-photon-excitation fluorescence imaging of three-dimensional calcium-ion activity. Appl Opt (1994) 1.15
Hydrodynamic models of viscous coupling between motile myosin and endoplasm in characean algae. J Cell Biol (1982) 1.11
Multiphoton-excited fluorescence of fluorogen-labeled neurotransmitters. Anal Chem (1996) 1.10
Studies on the increase in cytosolic free calcium induced by epidermal growth factor, serum, and nucleotides in individual A431 cells. J Cell Physiol (1988) 1.09
Fluorescence photobleaching recovery measurements of surface lateral mobilities on normal and SV40-transformed mouse fibroblasts. Biochemistry (1980) 1.07
Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence. Appl Opt (1997) 1.06
Macromolecular impurities and disorder in protein crystals. Proteins (1999) 1.05
Calcium sequestration in the Golgi apparatus of cultured mammalian cells revealed by laser scanning confocal microscopy and ion microscopy. J Cell Sci (1991) 1.03
Endosomal accumulation of pH indicator dyes delivered as acetoxymethyl esters. J Exp Biol (1994) 1.03
Measurement of lateral transport on cell surfaces. Prog Clin Biol Res (1976) 1.02
Psychological sequelae to bacterial meningitis: two controlled studies. Pediatrics (1972) 1.01
Barrier filter for fluorescence microscopy of strongly autofluorescent plant tissues. Application to actin cables in Chara. J Histochem Cytochem (1979) 0.99
Fluorescent erythrosin B is preferable to trypan blue as a vital exclusion dye for mammalian cells in monolayer culture. J Histochem Cytochem (1984) 0.99
Disparate modulation of plasma membrane protein lateral mobility by various cell permeabilizing agents. J Cell Physiol (1994) 0.96