Soluble expression, purification, and characterization of active recombinant human tissue plasminogen activator by auto-induction in E. coli.

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Published in BMC Biotechnol on March 01, 2015

Authors

Xiaobin Long1,2, Yeran Gou3,4, Miao Luo5,6,7, Shaocheng Zhang8,9, Hongpeng Zhang10,11, Lei Bai12,13, Shuang Wu14,15, Quan He16, Ke Chen17, Ailong Huang18, Jianzhong Zhou19, Deqiang Wang20,21

Author Affiliations

1: Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. 582526247@qq.com.
2: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. 582526247@qq.com.
3: Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. 522689024@qq.com.
4: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. 522689024@qq.com.
5: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. 536513878@qq.com.
6: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. 536513878@qq.com.
7: Department of Clinical Laboratory, Yubei District People's Hospital, Chongqing, 401120, PR China. 536513878@qq.com.
8: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. 125425439@qq.com.
9: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. 125425439@qq.com.
10: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. 610627111@qq.com.
11: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. 610627111@qq.com.
12: Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. 411351709@qq.com.
13: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. 411351709@qq.com.
14: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. 395480829@qq.com.
15: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. 395480829@qq.com.
16: Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. hequan822@yahoo.cn.
17: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. 378793028@qq.com.
18: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. ahuang1964@163.com.
19: Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. zjzcqu@163.com.
20: Key Laboratory of Molecular Biology on Infectious Disease (Ministry of Education), The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China. wangd@ustc.edu.
21: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. wangd@ustc.edu.

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