Strategies to generate high-titer, high-potency recombinant AAV3 serotype vectors.

PubWeight™: 0.76‹?›

🔗 View Article (PMID 27200382)

Published in Mol Ther Methods Clin Dev on May 04, 2016

Authors

Chen Ling1, Zifei Yin2, Jun Li2, Daniel Zhang2, George Aslanidi3, Arun Srivastava4

Author Affiliations

1: Division of Cellular and Molecular Therapy, Department of Pediatrics; University of Florida College of Medicine, Gainesville, Florida, USA; Powell Gene Therapy Center; University of Florida College of Medicine, Gainesville, Florida, USA; Shands Cancer Center; University of Florida College of Medicine, Gainesville, Florida, USA.
2: Division of Cellular and Molecular Therapy, Department of Pediatrics; University of Florida College of Medicine , Gainesville, Florida, USA.
3: Division of Cellular and Molecular Therapy, Department of Pediatrics; University of Florida College of Medicine, Gainesville, Florida, USA; Powell Gene Therapy Center; University of Florida College of Medicine, Gainesville, Florida, USA.
4: Division of Cellular and Molecular Therapy, Department of Pediatrics; University of Florida College of Medicine, Gainesville, Florida, USA; Powell Gene Therapy Center; University of Florida College of Medicine, Gainesville, Florida, USA; Shands Cancer Center; University of Florida College of Medicine, Gainesville, Florida, USA; Genetics Institute; University of Florida College of Medicine, Gainesville, Florida, USA; Department of Molecular Genetics & Microbiology; University of Florida College of Medicine, Gainesville, Florida, USA.

Articles cited by this

Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol (1967) 106.91

Adenovirus-associated virus vector-mediated gene transfer in hemophilia B. N Engl J Med (2011) 11.01

Persistent and therapeutic concentrations of human factor IX in mice after hepatic gene transfer of recombinant AAV vectors. Nat Genet (1997) 4.72

Next generation of adeno-associated virus 2 vectors: point mutations in tyrosines lead to high-efficiency transduction at lower doses. Proc Natl Acad Sci U S A (2008) 3.84

Recurrent AAV2-related insertional mutagenesis in human hepatocellular carcinomas. Nat Genet (2015) 1.86

Selection and evaluation of clinically relevant AAV variants in a xenograft liver model. Nature (2013) 1.80

Adeno-associated virus 2-mediated gene transfer in vivo: organ-tropism and expression of transduced sequences in mice. Gene (1997) 1.60

Adeno-associated virus type 2-mediated gene transfer: role of cellular FKBP52 protein in transgene expression. J Virol (2001) 1.58

Superior In vivo Transduction of Human Hepatocytes Using Engineered AAV3 Capsid. Mol Ther (2016) 1.54

Self-complementary recombinant adeno-associated viral vectors: packaging capacity and the role of rep proteins in vector purity. Hum Gene Ther (2007) 1.44

Rescue and replication of adeno-associated virus type 2 as well as vector DNA sequences from recombinant plasmids containing deletions in the viral inverted terminal repeats: selective encapsidation of viral genomes in progeny virions. J Virol (1996) 1.40

Liver transduction with recombinant adeno-associated virus is primarily restricted by capsid serotype not vector genotype. J Virol (2006) 1.36

Adeno-associated virus type 2 DNA replication in vivo: mutation analyses of the D sequence in viral inverted terminal repeats. J Virol (1997) 1.27

Human hepatocyte growth factor receptor is a cellular coreceptor for adeno-associated virus serotype 3. Hum Gene Ther (2010) 1.26

Cytotoxic genes from traditional Chinese medicine inhibit tumor growth both in vitro and in vivo. J Integr Med (2014) 1.26

Adeno-Associated Virus Type 2 and Hepatocellular Carcinoma? Hum Gene Ther (2015) 1.25

Optimization of the capsid of recombinant adeno-associated virus 2 (AAV2) vectors: the final threshold? PLoS One (2013) 1.24

Rescue and replication signals of the adeno-associated virus 2 genome. J Mol Biol (1995) 1.17

AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol (2014) 1.13

Strategies for improving the transduction efficiency of single-stranded adeno-associated virus vectors in vitro and in vivo. Gene Ther (2008) 1.07

Development of optimized AAV3 serotype vectors: mechanism of high-efficiency transduction of human liver cancer cells. Gene Ther (2011) 1.06

Native molecular state of adeno-associated viral vectors revealed by single-molecule sequencing. Hum Gene Ther (2011) 1.05

Limitations of encapsidation of recombinant self-complementary adeno-associated viral genomes in different serotype capsids and their quantitation. Hum Gene Ther Methods (2012) 1.04

AAV3-mediated transfer and expression of the pyruvate dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of human liver cancer cells. Mol Genet Metab (2009) 1.03

Comparative Study of Liver Gene Transfer With AAV Vectors Based on Natural and Engineered AAV Capsids. Mol Ther (2015) 1.03

Efficient and Targeted Transduction of Nonhuman Primate Liver With Systemically Delivered Optimized AAV3B Vectors. Mol Ther (2015) 1.02

Adeno-associated Vector Toxicity-To Be or Not to Be? Mol Ther (2015) 0.98

High-efficiency transduction of liver cancer cells by recombinant adeno-associated virus serotype 3 vectors. J Vis Exp (2011) 0.93

A concept of eliminating nonhomologous recombination for scalable and safe AAV vector generation for human gene therapy. Nucleic Acids Res (2013) 0.92

Selective in vivo targeting of human liver tumors by optimized AAV3 vectors in a murine xenograft model. Hum Gene Ther (2014) 0.91

Efficient production of dual recombinant adeno-associated viral vectors for factor VIII delivery. Hum Gene Ther Methods (2014) 0.91

E Pluribus Unum: 50 Years of Research, Millions of Viruses, and One Goal-Tailored Acceleration of AAV Evolution. Mol Ther (2015) 0.90

Enhanced transgene expression from recombinant single-stranded D-sequence-substituted adeno-associated virus vectors in human cell lines in vitro and in murine hepatocytes in vivo. J Virol (2014) 0.89

Proteomics analysis of co-purifying cellular proteins associated with rAAV vectors. PLoS One (2014) 0.87

Rationally designed capsid and transgene cassette of AAV6 vectors for dendritic cell-based cancer immunotherapy. Immunol Cell Biol (2013) 0.87

Prevalence of neutralizing antibodies against liver-tropic adeno-associated virus serotype vectors in 100 healthy Chinese and its potential relation to body constitutions. J Integr Med (2015) 0.85

Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program. J Biol Chem (2013) 0.85

LW-213 induces G2/M cell cycle arrest through AKT/GSK3β/β-catenin signaling pathway in human breast cancer cells. Mol Carcinog (2015) 0.84

Identification of small molecule inhibitors of Zcchc11 TUTase activity. RNA Biol (2015) 0.84

The overexpression and nuclear translocation of Trx-1 during hypoxia confers on HepG2 cells resistance to DDP, and GL-V9 reverses the resistance by suppressing the Trx-1/Ref-1 axis. Free Radic Biol Med (2015) 0.83

The Adeno-Associated Virus Genome Packaging Puzzle. J Mol Genet Med (2015) 0.80

Productive life cycle of adeno-associated virus serotype 2 in the complete absence of a conventional polyadenylation signal. J Gen Virol (2015) 0.78

Directed Differentiation of Embryonic Stem Cells Into Cardiomyocytes by Bacterial Injection of Defined Transcription Factors. Sci Rep (2015) 0.77

Construction and gene expression analysis of a single-stranded DNA minivector based on an inverted terminal repeat of adeno-associated virus. Mol Biotechnol (2015) 0.76