Published in Methods Enzymol on January 01, 1985
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Isoprenoid modification of rab proteins terminating in CC or CXC motifs. Proc Natl Acad Sci U S A (1991) 1.99
Enzymatic synthesis of isoprene from dimethylallyl diphosphate in aspen leaf extracts. Plant Physiol (1991) 1.82
Geranyl diphosphate synthase: cloning, expression, and characterization of this prenyltransferase as a heterodimer. Proc Natl Acad Sci U S A (1999) 1.61
Evolution of the isoprene biosynthetic pathway in kudzu. Plant Physiol (2005) 1.40
Kinetic and spectroscopic characterization of type II isopentenyl diphosphate isomerase from Thermus thermophilus: evidence for formation of substrate-induced flavin species. Biochemistry (2007) 1.35
Biochemical characterization of a spearmint mutant that resembles peppermint in monoterpene content. Plant Physiol (1991) 1.34
Inhibition of the Fe(4)S(4)-cluster-containing protein IspH (LytB): electron paramagnetic resonance, metallacycles, and mechanisms. J Am Chem Soc (2010) 1.21
Farnesyl diphosphate analogues with omega-bioorthogonal azide and alkyne functional groups for protein farnesyl transferase-catalyzed ligation reactions. J Org Chem (2007) 1.21
Polyprenyl phosphate biosynthesis in Mycobacterium tuberculosis and Mycobacterium smegmatis. J Bacteriol (2000) 1.20
Mycobacterial lipid II is composed of a complex mixture of modified muramyl and peptide moieties linked to decaprenyl phosphate. J Bacteriol (2005) 1.13
Evidence for the involvement of acid/base chemistry in the reaction catalyzed by the type II isopentenyl diphosphate/dimethylallyl diphosphate isomerase from Staphylococcus aureus. Biochemistry (2008) 1.11
Characterization and mechanistic studies of type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase from Staphylococcus aureus. Biochemistry (2007) 1.11
Differential accumulation of dimethylallyl diphosphate in leaves and needles of isoprene- and methylbutenol-emitting and nonemitting species. Plant Physiol (2002) 0.99
Decaprenyl diphosphate synthesis in Mycobacterium tuberculosis. J Bacteriol (2004) 0.97
Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton. Chembiochem (2013) 0.94
Elicitation of Diterpene Biosynthesis in Rice (Oryza sativa L.) by Chitin. Plant Physiol (1992) 0.93
Cloning, solubilization, and characterization of squalene synthase from Thermosynechococcus elongatus BP-1. J Bacteriol (2008) 0.92
The structural basis of chain length control in Rv1086. J Mol Biol (2008) 0.91
Stimulation of Isopentenyl Pyrophosphate Incorporation into Polyisoprene in Extracts from Guayule Plants (Parthenium argentatum Gray) by Low Temperature and 2-(3,4-Dichlorophenoxy) Triethylamine. Plant Physiol (1989) 0.84
Enantioselective inhibition of squalene synthase by aziridine analogues of presqualene diphosphate. J Org Chem (2010) 0.83
A new method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling. J Plant Res (2003) 0.81
A method for separation and determination of cytokinin nucleotides from plant tissues. J Plant Res (2003) 0.81
The Enzymatic Synthesis of Rubber Polymer in Parthenium argentatum Gray. Plant Physiol (1990) 0.80
Functional analysis of (4S)-limonene synthase mutants reveals determinants of catalytic outcome in a model monoterpene synthase. Proc Natl Acad Sci U S A (2015) 0.80
Product chain-length determination mechanism of Z,E-farnesyl diphosphate synthase. Biochem Biophys Res Commun (2008) 0.79
Functional characterization of the promiscuous prenyltransferase responsible for furaquinocin biosynthesis: identification of a physiological polyketide substrate and its prenylated reaction products. J Biol Chem (2010) 0.79
Clomazone does not inhibit the conversion of isopentenyl pyrophosphate to geranyl, farnesyl, or geranylgeranyl pyrophosphate in vitro. Plant Physiol (1992) 0.78
Molecular characterization and phylogenetic analysis of two novel regio-specific flavonoid prenyltransferases from Morus alba and Cudrania tricuspidata. J Biol Chem (2014) 0.77
Synthetic Routes to Methylerythritol Phosphate Pathway Intermediates and Downstream Isoprenoids. Curr Org Chem (2014) 0.76
Exploration of GGTase-I substrate requirements. Part 1: Synthesis and biochemical evaluation of novel aryl-modified geranylgeranyl diphosphate analogs. Bioorg Med Chem Lett (2016) 0.75
Exploration of GGTase-I substrate requirements. Part 2: Synthesis and biochemical analysis of novel saturated geranylgeranyl diphosphate analogs. Bioorg Med Chem Lett (2016) 0.75
Triterpenoid profiling and functional characterization of the initial genes involved in isoprenoid biosynthesis in neem (Azadirachta indica). BMC Plant Biol (2015) 0.75
Molecular cloning and biochemical characterization of isoprene synthases from the tropical trees Ficus virgata, Ficus septica, and Casuarina equisetifolia. J Plant Res (2015) 0.75
Creating isoprenoid diversity. Science (1997) 3.78
Structure-activity relationships for inhibition of farnesyl diphosphate synthase in vitro and inhibition of bone resorption in vivo by nitrogen-containing bisphosphonates. J Pharmacol Exp Ther (2001) 3.59
Crystal structure of recombinant farnesyl diphosphate synthase at 2.6-A resolution. Biochemistry (1994) 3.10
Regulation of product chain length by isoprenyl diphosphate synthases. Proc Natl Acad Sci U S A (1996) 2.13
The crystal structure of GMP synthetase reveals a novel catalytic triad and is a structural paradigm for two enzyme families. Nat Struct Biol (1996) 2.11
Enzymatic coupling of cholesterol intermediates to a mating pheromone precursor and to the ras protein. Science (1990) 2.04
Isoprenyl diphosphate synthases: protein sequence comparisons, a phylogenetic tree, and predictions of secondary structure. Protein Sci (1994) 1.82
Chain elongation in the isoprenoid biosynthetic pathway. Curr Opin Chem Biol (1997) 1.78
Yeast farnesyl-diphosphate synthase: site-directed mutagenesis of residues in highly conserved prenyltransferase domains I and II. Proc Natl Acad Sci U S A (1994) 1.69
Cloning, expression, and characterization of (+)-delta-cadinene synthase: a catalyst for cotton phytoalexin biosynthesis. Arch Biochem Biophys (1995) 1.64
Escherichia coli open reading frame 696 is idi, a nonessential gene encoding isopentenyl diphosphate isomerase. J Bacteriol (1999) 1.64
Isopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae. J Biol Chem (1989) 1.56
The Claviceps purpurea gene encoding dimethylallyltryptophan synthase, the committed step for ergot alkaloid biosynthesis. Biochem Biophys Res Commun (1995) 1.51
Farnesyl diphosphate synthetase. Molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiae. J Biol Chem (1989) 1.48
Imidazole glycerol phosphate synthase: the glutamine amidotransferase in histidine biosynthesis. Biochemistry (1993) 1.45
N5-carboxyaminoimidazole ribonucleotide: evidence for a new intermediate and two new enzymatic activities in the de novo purine biosynthetic pathway of Escherichia coli. Biochemistry (1994) 1.43
The crtE gene in Erwinia herbicola encodes geranylgeranyl diphosphate synthase. Proc Natl Acad Sci U S A (1992) 1.42
1H-15N NMR studies of Escherichia coli tRNA(Phe) from hisT mutants: a structural role for pseudouridine. Biochemistry (1991) 1.38
Effects of site-directed mutagenesis of the highly conserved aspartate residues in domain II of farnesyl diphosphate synthase activity. J Biol Chem (1992) 1.35
Crystal structure of human thymidylate synthase: a structural mechanism for guiding substrates into the active site. Biochemistry (1995) 1.32
E. coli MEP synthase: steady-state kinetic analysis and substrate binding. Biochemistry (2002) 1.32
Crystal structure of imidazole glycerol phosphate synthase: a tunnel through a (beta/alpha)8 barrel joins two active sites. Structure (2001) 1.32
Isopentenyl-diphosphate isomerase: inactivation of the enzyme with active-site-directed irreversible inhibitors and transition-state analogues. Biochemistry (1988) 1.32
Continuous fluorescence assay for protein prenyltransferases. Methods Enzymol (1995) 1.27
15N-labeled Escherichia coli tRNAfMet, tRNAGlu, tRNATyr, and tRNAPhe. Double resonance and two-dimensional NMR of N1-labeled pseudouridine. J Biol Chem (1985) 1.27
The CaaX proteases, Afc1p and Rce1p, have overlapping but distinct substrate specificities. Mol Cell Biol (2000) 1.22
Pre-steady-state study of recombinant sesquiterpene cyclases. Biochemistry (1997) 1.22
1-Deoxy-D-xylulose 5-phosphate synthase, the gene product of open reading frame (ORF) 2816 and ORF 2895 in Rhodobacter capsulatus. J Bacteriol (2001) 1.19
Studies with recombinant Saccharomyces cerevisiae CaaX prenyl protease Rce1p. Biochemistry (2000) 1.18
A mechanism for posttranslational modifications of proteins by yeast protein farnesyltransferase. Proc Natl Acad Sci U S A (1995) 1.18
Isopentenyldiphosphate:dimethylallyldiphosphate isomerase: construction of a high-level heterologous expression system for the gene from Saccharomyces cerevisiae and identification of an active-site nucleophile. Biochemistry (1990) 1.17
Cloning and heterologous expression of a second (+)-delta-cadinene synthase from Gossypium arboreum. J Nat Prod (1996) 1.16
Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms. Biochemistry (1994) 1.15
Purification and characterization of farnesyl diphosphate/geranylgeranyl diphosphate synthase. A thermostable bifunctional enzyme from Methanobacterium thermoautotrophicum. J Biol Chem (1993) 1.14
Prenyltransferase; determination of the binding mechanism and individual kinetic constants for farnesylpyrophosphate synthetase by rapid quench and isotope partitioning experiments. Biochemistry (1981) 1.14
Identification of Cys139 and Glu207 as catalytically important groups in the active site of isopentenyl diphosphate:dimethylallyl diphosphate isomerase. Biochemistry (1994) 1.13
Isolation and characterization of isoprene mutants of Escherichia coli. J Bacteriol (1989) 1.13
Mechanism for acivicin inactivation of triad glutamine amidotransferases. Biochemistry (2001) 1.12
Farnesyl pyrophosphate synthetase. Mechanistic studies of the 1'-4 coupling reaction with 2-fluorogeranyl pyrophosphate. J Biol Chem (1978) 1.11
Open reading frame 176 in the photosynthesis gene cluster of Rhodobacter capsulatus encodes idi, a gene for isopentenyl diphosphate isomerase. J Bacteriol (1996) 1.08
Yeast squalene synthase: expression, purification, and characterization of soluble recombinant enzyme. Arch Biochem Biophys (1993) 1.08
Virulence of a phosphoribosylaminoimidazole carboxylase-deficient Candida albicans strain in an immunosuppressed murine model of systemic candidiasis. Infect Immun (2001) 1.06
Chrysanthemyl diphosphate synthase: isolation of the gene and characterization of the recombinant non-head-to-tail monoterpene synthase from Chrysanthemum cinerariaefolium. Proc Natl Acad Sci U S A (2001) 1.06
Analysis of the isopentenyl diphosphate isomerase gene family from Arabidopsis thaliana. Plant Mol Biol (1998) 1.04
Multiple quantum two-dimensional 1H--15N nuclear magnetic resonance spectroscopy: chemical shift correlation maps for exchangeable imino protons of Escherichia coli tRNAMetf in water. Proc Natl Acad Sci U S A (1983) 1.03
Purification and characterization of dimethylallyl tryptophan synthase from Claviceps purpurea. Arch Biochem Biophys (1992) 1.03
Analysis and purification of phosphorylated isoprenoids by reversed-phase HPLC. Anal Biochem (1993) 1.03
Cloning, sequencing, and expression of the Lactobacillus casei thymidylate synthase gene. DNA (1988) 1.02
Carboxylases in de novo purine biosynthesis. Characterization of the Gallus gallus bifunctional enzyme. Biochemistry (1994) 1.02
Human isopentenyl diphosphate: dimethylallyl diphosphate isomerase: overproduction, purification, and characterization. Arch Biochem Biophys (1996) 1.02
Biochemical role of the Cryptococcus neoformans ADE2 protein in fungal de novo purine biosynthesis. Arch Biochem Biophys (1998) 1.01
Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: essential elements for recognition of tRNA substrates within the anticodon stem-loop. Biochemistry (2000) 1.01
Synthesis of (S)-isoprenoid thiodiphosphates as substrates and inhibitors. J Org Chem (2001) 0.99
Mechanism of the prenyl-transfer reaction. Studies with (E)- and (Z)-3-trifluoromethyl-2-buten-1-yl pyrophosphate. Biochemistry (1977) 0.98
Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: site-directed mutagenesis of highly conserved residues. Biochemistry (2001) 0.97
Protein farnesyltransferase: production in Escherichia coli and immunoaffinity purification of the heterodimer from Saccharomyces cerevisiae. Gene (1993) 0.97
Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: a binding mechanism for recombinant enzyme. Biochemistry (1997) 0.96
Disruption and mapping of IDI1, the gene for isopentenyl diphosphate isomerase in Saccharomyces cerevisiae. Yeast (1992) 0.95
Squalene synthase: steady-state, pre-steady-state, and isotope-trapping studies. Biochemistry (2000) 0.95
Stereostructure of the archaebacterial C40 diol. Science (1985) 0.95
Geranylgeranylglyceryl phosphate synthase. Characterization of the recombinant enzyme from Methanobacterium thermoautotrophicum. Biochemistry (2001) 0.94
Crystal structure of the C67A mutant of isopentenyl diphosphate isomerase complexed with a mechanism-based irreversible inhibitor. Proteins (2004) 0.94
Farnesyl protein transferase: identification of K164 alpha and Y300 beta as catalytic residues by mutagenesis and kinetic studies. Biochemistry (1999) 0.93
Subunit interactions and glutamine utilization by Escherichia coli imidazole glycerol phosphate synthase. J Bacteriol (2001) 0.93
Isolation and characterization of idsA: the gene for the short chain isoprenyl diphosphate synthase from Methanobacterium thermoautotrophicum. Arch Biochem Biophys (1994) 0.93
Isopentenyl-diphosphate isomerase: irreversible inhibition by 3-methyl-3,4-epoxybutyl diphosphate. Biochemistry (1992) 0.93
Prenyltransferase: the mechanism of the reaction. Biochemistry (1976) 0.92
The purification and steady-state kinetic behaviour of rabbit heart mitochondrial NAD(P)+ malic enzyme. Biochem J (1985) 0.90
Purification of farnesylpyrophosphate synthetase by affinity chromatography. Methods Enzymol (1985) 0.90
Isolation of Schizosaccharomyces pombe isopentenyl diphosphate isomerase cDNA clones by complementation and synthesis of the enzyme in Escherichia coli. J Biol Chem (1995) 0.90
Yeast protein farnesyltransferase: steady-state kinetic studies of substrate binding. Biochemistry (1995) 0.90
Site-directed mutagenesis of arginine 179 of thymidylate synthase. A nonessential substrate-binding residue. J Biol Chem (1990) 0.89
Yeast protein farnesyltransferase. pKas of peptide substrates bound as zinc thiolates. Biochemistry (1999) 0.89
Template directed incorporation of nucleotide mixtures using azole-nucleobase analogs. Nucleic Acids Res (1997) 0.89
Sequence specific cleavage of messenger RNA by a modified ribonuclease H. Bioorg Med Chem (1994) 0.88
Biosynthesis of isoprenoids in Escherichia coli: stereochemistry of the reaction catalyzed by farnesyl diphosphate synthase. Org Lett (1999) 0.88
Synthesis of 2-C-methyl-D-erythritol 4-phosphate: the first pathway-specific intermediate in the methylerythritol phosphate route to isoprenoids. Org Lett (2000) 0.87
Absence of substrate channeling between active sites in the Agrobacterium tumefaciens IspDF and IspE enzymes of the methyl erythritol phosphate pathway. Biochemistry (2006) 0.87
(S)-geranylgeranylglyceryl phosphate synthase. Purification and characterization of the first pathway-specific enzyme in archaebacterial membrane lipid biosynthesis. J Biol Chem (1993) 0.87
Prenyltransferase. Kinetic studies of the 1'-4 coupling reaction with avian liver enzyme. J Biol Chem (1979) 0.87
Solid-phase synthesis of a farnesylated CaaX peptide library: inhibitors of the Ras CaaX endoprotease. J Comb Chem (2000) 0.87
Yeast protein farnesyltransferase: a pre-steady-state kinetic analysis. Biochemistry (1997) 0.86
Expression and purification of imidazole glycerol phosphate synthase from Saccharomyces cerevisiae. Protein Expr Purif (2000) 0.86
NMR structure of a DNA duplex containing nucleoside analog 1-(2'-deoxy-beta-D-ribofuranosyl)-3-nitropyrrole and the structure of the unmodified control. Nucleic Acids Res (2000) 0.86
Farnesyl diphosphate synthase. Altering the catalytic site to select for geranyl diphosphate activity. Biochemistry (2000) 0.85
Exploratory studies on azole carboxamides as nucleobase analogs: thermal denaturation studies on oligodeoxyribonucleotide duplexes containing pyrrole-3-carboxamide. Nucleic Acids Res (1998) 0.84
Preliminary X-ray analysis of Escherichia coli GMP synthetase: determination of anomalous scattering factors for a cysteinyl mercury derivative. Proteins (1994) 0.83
Direct characterization of enzyme-substrate complexes by using electrosonic spray ionization mass spectrometry. Angew Chem Int Ed Engl (2005) 0.83
Mechanistic studies with 2-C-methyl-D-erythritol 4-phosphate synthase from Escherichia coli. Biochemistry (2005) 0.83
Purification and characterization of recombinant human farnesyl diphosphate synthase expressed in Escherichia coli. Biochem J (1991) 0.82
Efficient syntheses of [3-15N]uracil and [3-15N]thymine. Nucleic Acids Res (1983) 0.82
N1-(5'-phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase: purification and characterization of a unique metalloenzyme. Biochemistry (1999) 0.81
Recent studies of the mechanism of protein prenylation. Nat Prod Rep (2000) 0.81
Letter: Prenyltransferase. New evidence for an ionization-condensation-elimination mechanism with 2-fluorogeranyl pyrophosphate. J Am Chem Soc (1977) 0.81
Cloning, sequence analysis and expression of the gene encoding imidazole glycerol phosphate dehydratase in Cryptococcus neoformans. Gene (1994) 0.80
Yeast protein geranylgeranyltransferase type-I: overproduction, purification, and characterization. Arch Biochem Biophys (1995) 0.80
15N-labeled tRNA. Identification of 4-thiouridine in Escherichia coli tRNASer1 and tRNATyr2 by 1H-15N two-dimensional NMR spectroscopy. J Biol Chem (1986) 0.80
Yeast squalene synthase. A mechanism for addition of substrates and activation by NADPH. J Biol Chem (1994) 0.79
Synthesis of (R)-[2-2H]isopentenyl diphosphate and determination of its enantiopurity by 2H NMR spectroscopy in a lyotropic medium. Org Lett (1999) 0.79