Published in Proc Natl Acad Sci U S A on December 01, 1988
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Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol (1994) 9.97
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Characterization of the terminal regions of hepatitis C viral RNA: identification of conserved sequences in the 5' untranslated region and poly(A) tails at the 3' end. Proc Natl Acad Sci U S A (1991) 7.13
Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction. Appl Environ Microbiol (1992) 6.54
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Effective amplification of long targets from cloned inserts and human genomic DNA. Proc Natl Acad Sci U S A (1994) 4.86
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High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleic Acids Res (1990) 4.12
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Optimal conditions for directly sequencing double-stranded PCR products with sequenase. Nucleic Acids Res (1990) 3.90
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Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica. Proc Natl Acad Sci U S A (1991) 2.03
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The Fas protein is expressed at high levels on CD4+CD8+ thymocytes and activated mature lymphocytes in normal mice but not in the lupus-prone strain, MRL lpr/lpr. Proc Natl Acad Sci U S A (1993) 1.76
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Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation. Proc Natl Acad Sci U S A (1999) 1.67
Termination of DNA synthesis by N6-alkylated, not 3'-O-alkylated, photocleavable 2'-deoxyadenosine triphosphates. Nucleic Acids Res (2007) 1.64
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Factors affecting fidelity of DNA synthesis during PCR amplification of d(C-A)n.d(G-T)n microsatellite repeats. Nucleic Acids Res (1996) 1.36
A mitochondrial DNA variant, identified in Leber hereditary optic neuropathy patients, which extends the amino acid sequence of cytochrome c oxidase subunit I. Am J Hum Genet (1992) 1.32
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Replication of poliovirus RNA containing two VPg coding sequences leads to a specific deletion event. J Virol (1993) 1.24
Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda. Proc Natl Acad Sci U S A (1989) 1.22
Characterization of the 5' to 3' exonuclease associated with Thermus aquaticus DNA polymerase. Nucleic Acids Res (1990) 1.22
A novel fluorescence-based assay for the rapid detection and quantification of cellular deoxyribonucleoside triphosphates. Nucleic Acids Res (2011) 1.21
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A fundamental study of the PCR amplification of GC-rich DNA templates. Comput Biol Chem (2008) 1.18
Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA. Nucleic Acids Res (1989) 1.17
Characterization of cis-acting mutations in the first attenuator region of the Bacillus subtilis pyr operon that are defective in pyrimidine-mediated regulation of expression. J Bacteriol (1996) 1.16
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Biochemical evidence for the requirement of Hoogsteen base pairing for replication by human DNA polymerase iota. Proc Natl Acad Sci U S A (2005) 1.09
Precursor polyprotein for multiple neuropeptides secreted from the suboesophageal ganglion of the silkworm Bombyx mori: characterization of the cDNA encoding the diapause hormone precursor and identification of additional peptides. Proc Natl Acad Sci U S A (1993) 1.09
Semi exponential cycle sequencing. Nucleic Acids Res (1995) 1.08
A translation-attenuating intraleader open reading frame is selected on coronavirus mRNAs during persistent infection. Proc Natl Acad Sci U S A (1993) 1.07
Cloning, sequencing, and expression of the Zymomonas mobilis fructokinase gene and structural comparison of the enzyme with other hexose kinases. J Bacteriol (1992) 1.06
Random mutagenesis of two complementarity determining region amino acids yields an unexpectedly high frequency of antibodies with increased affinity for both cognate antigen and autoantigen. J Exp Med (1995) 1.05
Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum. Nucleic Acids Res (1992) 1.04
The mitochondrial tyrosyl-tRNA synthetase of Podospora anserina is a bifunctional enzyme active in protein synthesis and RNA splicing. Mol Cell Biol (1992) 1.02
Mutational analysis of the ICP4 binding sites in the 5' transcribed noncoding domains of the herpes simplex virus 1 UL 49.5 gamma 2 gene. J Virol (1992) 1.01
'Sticky feet'-directed mutagenesis and its application to swapping antibody domains. Nucleic Acids Res (1989) 1.01
Intragenic recombination and a chimeric outer membrane protein in the relapsing fever agent Borrelia hermsii. J Bacteriol (1993) 1.01
Nucleic acid-based fluorescent probes and their analytical potential. Anal Bioanal Chem (2010) 1.00
Mutation detection with MutH, MutL, and MutS mismatch repair proteins. Proc Natl Acad Sci U S A (1996) 1.00
Chromosomal deletion and rearrangement in Streptomyces glaucescens. J Bacteriol (1991) 0.99
Polymorphism of apolipoprotein A-II (apoA-II) among inbred strains of mice. Relationship between the molecular type of apoA-II and mouse senile amyloidosis. Biochem J (1991) 0.98
Helix-hairpin-helix motifs confer salt resistance and processivity on chimeric DNA polymerases. Proc Natl Acad Sci U S A (2002) 0.98
Rapid genotyping of hepatitis C virus by primer-specific extension analysis. J Clin Microbiol (2005) 0.98
Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution. Nucleic Acids Res (2004) 0.97
Substrate and mispairing properties of 5-formyl-2'-deoxyuridine 5'-triphosphate assessed by in vitro DNA polymerase reactions. Nucleic Acids Res (1997) 0.97
Molecular characterization of four different classes of mutations in the isovaleryl-CoA dehydrogenase gene responsible for isovaleric acidemia. Am J Hum Genet (1991) 0.95
DNA polymerases drive DNA sequencing-by-synthesis technologies: both past and present. Front Microbiol (2014) 0.95
Demystified ... the polymerase chain reaction. Mol Pathol (1999) 0.93
A 5' untranslated region which directs accurate and robust translation by prokaryotic and mammalian ribosomes. Nucleic Acids Res (1996) 0.92
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Rearrangement and junctional-site sequence analyses of T-cell receptor gamma genes in intestinal intraepithelial lymphocytes from murine athymic chimeras. Mol Cell Biol (1991) 0.91
Familial chylomicronemia (type I hyperlipoproteinemia) due to a single missense mutation in the lipoprotein lipase gene. J Clin Invest (1991) 0.90
Exoquence DNA sequencing. Nucleic Acids Res (1993) 0.89
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ftsE(Ts) affects translocation of K+-pump proteins into the cytoplasmic membrane of Escherichia coli. J Bacteriol (1998) 0.88
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Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science (1985) 95.08
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A (1983) 54.64
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A (1987) 37.15
Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc Natl Acad Sci U S A (1988) 18.72
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