Published in Nature on July 14, 1977
Cloning vector system for Corynebacterium glutamicum. J Bacteriol (1985) 2.37
Genetics and molecular biology of Streptomyces bacteriophages. Microbiol Rev (1980) 2.32
Cloning of a pleiotropic gene that positively controls biosynthesis of A-factor, actinorhodin, and prodigiosin in Streptomyces coelicolor A3(2) and Streptomyces lividans. J Bacteriol (1983) 1.94
Cloning and expression in streptomyces lividans of antibiotic resistance genes derived from Escherichia coli. J Bacteriol (1981) 1.61
Computer-assisted chromosome mapping by protoplast fusion in Staphylococcus aureus. J Bacteriol (1983) 1.58
Restriction of bacteriophage plaque formation in Streptomyces spp. J Bacteriol (1984) 1.46
Site-specific insertion of biologically functional adventitious genes into the Streptomyces lividans chromosome. J Bacteriol (1988) 1.32
Properties of S-adenosyl-L-methionine:macrocin O-methyltransferase in extracts of Streptomyces fradiae strains which produce normal or elevated levels of tylosin and in mutants blocked in specific O-methylations. Antimicrob Agents Chemother (1981) 1.28
Genetics of antibiotic production. Bacteriol Rev (1977) 1.27
L-form bacteria, cell walls and the origins of life. Open Biol (2013) 1.26
High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycol. J Bacteriol (1979) 1.19
Electron microscopic study of Bacillus subtilis protoplast fusion. J Bacteriol (1979) 1.16
S-Adenosyl-L-methionine: macrocin O-methyltransferase activities in a series of Streptomyces fradiae mutants that produce different levels of the macrolide antibiotic tylosin. Antimicrob Agents Chemother (1982) 1.11
Bacterial fusion assayed by a prophage complementation test. J Bacteriol (1979) 1.07
Polyethylene glycol-induced fusion of heat-inactivated and living protoplasts of Bacillus megaterium. J Bacteriol (1978) 1.02
Actinomycin biosynthesis by protoplasts derived from Streptomyces parvulus. Antimicrob Agents Chemother (1978) 0.97
Reciprocal and nonreciprocal recombination in diploid clones from Bacillus subtilis protoplast fusion: Association with the replication origin and terminus. Proc Natl Acad Sci U S A (1983) 0.93
Optimal Cultural and Physiological Conditions for Handling Streptomyces rimosus Protoplasts. Appl Environ Microbiol (1982) 0.92
Efficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus. Appl Environ Microbiol (1987) 0.88
Genetic recombination in Micromonospora rosaria by protoplast fusion. Appl Environ Microbiol (1983) 0.87
Protoplast fusion permits high-frequency transfer of a Streptomyces determinant which mediates actinomycin synthesis. J Bacteriol (1982) 0.86
Transfection in Micromonospora spp. Appl Environ Microbiol (1987) 0.80
Evidence for a chromosomal location of the genes coding for chloramphenicol production in Streptomyces venezuelae. J Bacteriol (1983) 0.77
The falsifiability of the models for the origin of eukaryotes. Curr Genet (2011) 0.76
Genetic recombination in Actinoplanes brasiliensis by protoplast fusion. Appl Environ Microbiol (1983) 0.75
Radioimmunoassay of gentamicin in Micromonospora medium extracts. Antimicrob Agents Chemother (1980) 0.75
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol (1978) 75.38
Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A (1972) 57.06
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol (1980) 41.18
Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A (1973) 25.61
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A (1979) 22.59
Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature (2002) 19.35
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet (1979) 17.16
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol (1974) 15.89
Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Proc Natl Acad Sci U S A (1973) 13.45
Sequence and gene organization of mouse mitochondrial DNA. Cell (1981) 13.14
Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev (1976) 12.28
Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli. J Mol Biol (1970) 11.86
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol (1973) 11.40
Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A (1974) 10.51
Multiple molecular species of circular R-factor DNA isolated from Escherichia coli. Nature (1969) 9.02
Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A (1975) 8.85
Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. Nature (1978) 8.76
Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc Natl Acad Sci U S A (1975) 7.60
Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts. Proc Natl Acad Sci U S A (1983) 6.79
Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature (1979) 6.56
Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol (1983) 6.44
Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli. J Bacteriol (1986) 6.06
Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans. Mol Gen Genet (1982) 5.99
Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature (1976) 5.73
Transposable genetic elements and plasmid evolution. Nature (1976) 5.58
Revised interpretation of the origin of the pSC101 plasmid. J Bacteriol (1977) 5.51
Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A (1974) 5.47
Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J Bacteriol (1975) 5.35
Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J Gen Microbiol (1983) 5.06
pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet (1982) 5.02
Nonchromosomal antibiotic resistance in bacteria. V. Isolation and characterization of R factor mutants exhibiting temperature-sensitive repression of fertility. J Bacteriol (1972) 4.85
A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol Microbiol (1996) 4.81
Molecular and genetic studies of an R factor system consisting of independent transfer and drug resistance plasmids. J Bacteriol (1973) 4.81
Are chlamydial infections the most prevalent venereal disease? JAMA (1975) 4.79
Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev (1967) 4.78
Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique. J Bacteriol (1983) 4.56
Dengue and chikungunya virus infection in man in Thailand, 1962-1964. I. Observations on hospitalized patients with hemorrhagic fever. Am J Trop Med Hyg (1969) 4.41
Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator. Mol Gen Genet (1986) 4.41
Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol Gen Genet (1978) 4.33
Observations related to pathogenesis of dengue hemorrhagic fever. IV. Relation of disease severity to antibody response and virus recovered. Yale J Biol Med (1970) 4.30
Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons. Proc Natl Acad Sci U S A (1974) 4.27
Structure and genomic organization of the mouse dihydrofolate reductase gene. Cell (1980) 4.25
3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate. Gene (1980) 4.22
Differential expression of photosynthesis genes in R. capsulata results from segmental differences in stability within the polycistronic rxcA transcript. Cell (1985) 4.15
Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 element. J Bacteriol (1977) 4.08
Genetic studies with bacterial protoplasts. Annu Rev Microbiol (1981) 4.05
Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance. Cell (1980) 4.01
Isolation of catenated forms of R factor DNA from minicells. Nat New Biol (1971) 3.99
Gene cloning in Streptomyces. Curr Top Microbiol Immunol (1982) 3.95
Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. J Bacteriol (1975) 3.88
Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proc Natl Acad Sci U S A (1981) 3.77
Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature (1975) 3.70
Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids. J Mol Biol (1976) 3.63
Cloning and endonuclease mapping of the hepatitis B viral genome. Nature (1979) 3.62
ADD1/SREBP1 activates PPARgamma through the production of endogenous ligand. Proc Natl Acad Sci U S A (1998) 3.61
Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host. Nature (1984) 3.61
Letter: Potential biohazards of recombinant DNA molecules. Science (1974) 3.45
Penicillinase-producing Neisseria gonorrhoeae. Lancet (1976) 3.37
DNA cloning in Streptomyces: resistance genes from antibiotic-producing species. Nature (1980) 3.36
The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces. Cell (1991) 3.32
A sensitive radioimmunoassay for detecting products translated from cloned DNA fragments. Cell (1978) 3.30
Transformation of plasmid DNA into Streptomyces at high frequency. Nature (1978) 3.27
Hemorrhagic fevers of Southeast Asia and South America: a comparative appraisal. Prog Med Virol (1967) 3.22
Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene (1985) 3.17
Engineered biosynthesis of novel polyketides. Science (1993) 3.16
Codon usage in the G+C-rich Streptomyces genome. Gene (1992) 3.11
Cloning of antibiotic resistance and nutritional genes in streptomycetes. J Bacteriol (1982) 3.06
Growth-rate dependent regulation of mRNA stability in Escherichia coli. Nature (1984) 3.06
Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells. Cell (1996) 2.92
Mutangenesis by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) in Streptomyces coelicolor. Mutat Res (1970) 2.88
Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction. Gene (1982) 2.81
Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids. Cell (1975) 2.80
The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments. Cell (1986) 2.79
Chromosomal recombination and mapping in Rhizobium leguminosarum. Nature (1976) 2.78
Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1. Proc Natl Acad Sci U S A (1970) 2.76
In vivo site-specific genetic recombination promoted by the EcoRI restriction endonuclease. Proc Natl Acad Sci U S A (1977) 2.74
Loss-of-function mutations in PPAR gamma associated with human colon cancer. Mol Cell (1999) 2.71
Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces. Mol Gen Genet (1985) 2.71
Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor. Gene (1988) 2.68
Coryneform bacterial endocarditis: difficulties in diagnosis and treatment, presentation of three cases, and review of literature. Mayo Clin Proc (1977) 2.63
Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2). Mol Gen Genet (1986) 2.61
Advances in Streptomyces coelicolor genetics. Bacteriol Rev (1973) 2.57
Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits. Nature (1995) 2.56
The usefulness of preoperative laboratory screening. JAMA (1985) 2.55
An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability. Cell (1988) 2.53
Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays. Genes Dev (2001) 2.49
The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene (1985) 2.46
Cloning, disruption, and transcriptional analysis of three RNA polymerase sigma factor genes of Streptomyces coelicolor A3(2). J Bacteriol (1990) 2.46
Shock associated with dengue infection. I. Clinical and physiologic manifestations of dengue hemorrhagic fever in Thailand, 1964. J Pediatr (1966) 2.45