Published in Methods Enzymol on January 01, 1983
Ribosome pausing and stacking during translation of a eukaryotic mRNA. EMBO J (1988) 5.61
A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences. EMBO J (1984) 4.50
Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle. Proc Natl Acad Sci U S A (1986) 3.55
Elongation arrest is not a prerequisite for secretory protein translocation across the microsomal membrane. J Cell Biol (1985) 3.19
The most abundant small cytoplasmic RNA of Saccharomyces cerevisiae has an important function required for normal cell growth. Mol Cell Biol (1989) 2.55
Targeting of the hepatitis B virus precore protein to the endoplasmic reticulum membrane: after signal peptide cleavage translocation can be aborted and the product released into the cytoplasm. J Cell Biol (1988) 2.41
Protein export in Escherichia coli requires a soluble activity. Proc Natl Acad Sci U S A (1984) 2.34
The origin and maintenance of mammalian peroxisomes involves a de novo PEX16-dependent pathway from the ER. J Cell Biol (2006) 2.31
Direct probing of the interaction between the signal sequence of nascent preprolactin and the signal recognition particle by specific cross-linking. J Cell Biol (1987) 2.21
Signal recognition particle mediates a transient elongation arrest of preprolactin in reticulocyte lysate. J Cell Biol (1989) 2.13
The translocation, folding, assembly and redox-dependent degradation of secretory and membrane proteins in semi-permeabilized mammalian cells. Biochem J (1995) 1.98
The signal recognition particle receptor is a complex that contains two distinct polypeptide chains. J Cell Biol (1986) 1.95
Assembly of the Alu domain of the signal recognition particle (SRP): dimerization of the two protein components is required for efficient binding to SRP RNA. Mol Cell Biol (1990) 1.76
Protein translocation across the endoplasmic reticulum membrane: identification by photocross-linking of a 39-kD integral membrane glycoprotein as part of a putative translocation tunnel. J Cell Biol (1989) 1.70
Requirements for the membrane insertion of signal-anchor type proteins. J Cell Biol (1991) 1.59
Biogenesis of the crystalloid endoplasmic reticulum in UT-1 cells: evidence that newly formed endoplasmic reticulum emerges from the nuclear envelope. J Cell Biol (1986) 1.54
Protein translocation across wheat germ microsomal membranes requires an SRP-like component. EMBO J (1987) 1.51
Positive charges at the NH2 terminus convert the membrane-anchor signal peptide of cytochrome P-450 to a secretory signal peptide. Proc Natl Acad Sci U S A (1988) 1.47
Signal recognition particle mediates post-translational targeting in eukaryotes. EMBO J (2004) 1.46
Nascent polypeptide-associated complex protein prevents mistargeting of nascent chains to the endoplasmic reticulum. Proc Natl Acad Sci U S A (1995) 1.45
Evidence for an extended 7SL RNA structure in the signal recognition particle. EMBO J (1987) 1.44
The signal sequence receptor, unlike the signal recognition particle receptor, is not essential for protein translocation. J Cell Biol (1992) 1.40
The COOH-terminal ends of internal signal and signal-anchor sequences are positioned differently in the ER translocase. J Cell Biol (1994) 1.39
Antiribophorin antibodies inhibit the targeting to the ER membrane of ribosomes containing nascent secretory polypeptides. J Cell Biol (1990) 1.32
The interaction of the chaperonin tailless complex polypeptide 1 (TCP1) ring complex (TRiC) with ribosome-bound nascent chains examined using photo-cross-linking. J Cell Biol (2000) 1.25
The beta subunit of the signal recognition particle receptor is a transmembrane GTPase that anchors the alpha subunit, a peripheral membrane GTPase, to the endoplasmic reticulum membrane. J Cell Biol (1995) 1.23
GTPase domain of the 54-kD subunit of the mammalian signal recognition particle is required for protein translocation but not for signal sequence binding. J Cell Biol (1993) 1.22
Signal recognition particle is required for co-translational insertion of cytochrome P-450 into microsomal membranes. Proc Natl Acad Sci U S A (1984) 1.22
A two-step recognition of signal sequences determines the translocation efficiency of proteins. EMBO J (1996) 1.19
The SRP9/14 subunit of the signal recognition particle (SRP) is present in more than 20-fold excess over SRP in primate cells and exists primarily free but also in complex with small cytoplasmic Alu RNAs. Mol Biol Cell (1995) 1.18
The intrinsic ability of ribosomes to bind to endoplasmic reticulum membranes is regulated by signal recognition particle and nascent-polypeptide-associated complex. Proc Natl Acad Sci U S A (1995) 1.17
A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins. EMBO J (1995) 1.14
N-myristoylation determines dual targeting of mammalian NADH-cytochrome b5 reductase to ER and mitochondrial outer membranes by a mechanism of kinetic partitioning. J Cell Biol (2005) 1.14
Structure of the signal recognition particle by electron microscopy. Proc Natl Acad Sci U S A (1985) 1.13
A membrane component of the endoplasmic reticulum that may be essential for protein translocation. EMBO J (1989) 1.10
Ribosome binding to the endoplasmic reticulum: a 180-kD protein identified by crosslinking to membrane-bound ribosomes is not required for ribosome binding activity. J Cell Biol (1991) 1.09
Targeting and topology in the membrane of plant 3-hydroxy-3-methylglutaryl coenzyme A reductase. Plant Cell (1995) 1.09
A nascent membrane protein is located adjacent to ER membrane proteins throughout its integration and translation. J Cell Biol (1991) 1.09
Deletion analysis of the internal signal-anchor domain of the human asialoglycoprotein receptor H1. EMBO J (1987) 1.07
Full-length prepro-alpha-factor can be translocated across the mammalian microsomal membrane only if translation has not terminated. J Cell Biol (1988) 1.06
Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane. Mol Biol Cell (2006) 1.04
Isolation of a cDNA clone of the 14-kDa subunit of the signal recognition particle by cross-hybridization of differently primed polymerase chain reactions. Proc Natl Acad Sci U S A (1989) 1.00
Discrete nascent chain lengths are required for the insertion of presecretory proteins into microsomal membranes. J Cell Biol (1993) 0.98
A general mechanism for regulation of access to the translocon: competition for a membrane attachment site on ribosomes. Proc Natl Acad Sci U S A (1998) 0.94
Membrane topography and topogenesis of prenylated Rab acceptor (PRA1). J Biol Chem (2001) 0.93
Redundancy of signal and anchor functions in the NH2-terminal uncharged region of influenza virus neuraminidase, a class II membrane glycoprotein. J Virol (1988) 0.89
Human autoantibodies against the 54 kDa protein of the signal recognition particle block function at multiple stages. Arthritis Res Ther (2006) 0.88
Mff functions with Pex11pβ and DLP1 in peroxisomal fission. Biol Open (2013) 0.81
Post-translational transport of proteins into microsomal membranes of Candida maltosa. EMBO J (1988) 0.80
The code for directing proteins for translocation across ER membrane: SRP cotranslationally recognizes specific features of a signal sequence. J Mol Biol (2014) 0.80
Genetic and biochemical analysis of the fission yeast ribonucleoprotein particle containing a homolog of Srp54p. Nucleic Acids Res (1994) 0.79
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol (1975) 36.95
Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components. J Cell Biol (1975) 15.47
Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation. Cell (2000) 13.44
Intracellular protein topogenesis. Proc Natl Acad Sci U S A (1980) 13.40
Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature (1986) 11.35
Nuclei from rat liver: isolation method that combines purity with high yield. Science (1966) 10.35
Transcriptional induction of genes encoding endoplasmic reticulum resident proteins requires a transmembrane protein kinase. Cell (1993) 9.84
70K heat shock related proteins stimulate protein translocation into microsomes. Nature (1988) 9.60
Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes. J Cell Biol (1981) 8.36
A novel mechanism for regulating activity of a transcription factor that controls the unfolded protein response. Cell (1996) 8.17
Identification and characterization of a nuclear pore complex protein. Cell (1986) 7.93
Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components. J Cell Biol (1973) 7.92
The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature (1993) 7.80
Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum. Nature (1982) 7.80
The nuclear envelope lamina is reversibly depolymerized during mitosis. Cell (1980) 7.67
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J Cell Biol (1996) 7.64
Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein. J Cell Biol (1981) 7.40
Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum. Proc Natl Acad Sci U S A (1980) 7.17
Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell (1995) 6.97
Preparation of microsomal membranes for cotranslational protein translocation. Methods Enzymol (1983) 6.94
Dissociation of mammalian polyribosomes into subunits by puromycin. Proc Natl Acad Sci U S A (1971) 6.77
Protein translocation across the endoplasmic reticulum. Cell (1984) 6.69
Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution. J Cell Biol (1978) 6.29
The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response. Cell (1997) 6.26
In vitro synthesis and processing of a putative precursor for the small subunit of ribulose-1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A (1977) 5.89
Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes. J Cell Biol (1970) 5.72
Ribosome pausing and stacking during translation of a eukaryotic mRNA. EMBO J (1988) 5.61
Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein. Proc Natl Acad Sci U S A (1977) 5.51
Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA. Mol Biol Cell (1997) 5.47
Oligomerization and phosphorylation of the Ire1p kinase during intracellular signaling from the endoplasmic reticulum to the nucleus. EMBO J (1996) 5.46
Mechanism of protein translocation across the endoplasmic reticulum membrane. Annu Rev Cell Biol (1986) 5.28
Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha. Cell (1998) 5.27
Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast. Proc Natl Acad Sci U S A (1979) 5.21
Protein translocation across the endoplasmic reticulum. II. Isolation and characterization of the signal recognition particle receptor. J Cell Biol (1982) 5.20
Homology in amino-terminal sequence of precursors to pancreatic secretory proteins. Proc Natl Acad Sci U S A (1975) 5.15
Controlled proteolysis of nascent polypeptides in rat liver cell fractions. I. Location of the polypeptides within ribosomes. J Cell Biol (1970) 5.08
Intracellular signaling from the endoplasmic reticulum to the nucleus: the unfolded protein response in yeast and mammals. Curr Opin Cell Biol (2001) 5.08
cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins. Proc Natl Acad Sci U S A (1986) 5.07
Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc Natl Acad Sci U S A (1995) 5.04
Structure of the 80S ribosome from Saccharomyces cerevisiae--tRNA-ribosome and subunit-subunit interactions. Cell (2001) 5.01
tRNA ligase is required for regulated mRNA splicing in the unfolded protein response. Cell (1996) 4.93
The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer. EMBO J (1997) 4.80
Translocation of proteins across the endoplasmic reticulum. II. Signal recognition protein (SRP) mediates the selective binding to microsomal membranes of in-vitro-assembled polysomes synthesizing secretory protein. J Cell Biol (1981) 4.80
Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA. J Mol Biol (1967) 4.71
A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei. J Cell Biol (1976) 4.64
In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-alpha-factor. Cell (1986) 4.59
Cloning of the human estrogen receptor cDNA. Proc Natl Acad Sci U S A (1985) 4.58
The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex. Cell (1995) 4.55
Translocation of proteins across membranes: the signal hypothesis and beyond. Symp Soc Exp Biol (1979) 4.54
Cell-free translation of messenger RNA in a wheat germ system. Methods Enzymol (1983) 4.52
Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. J Biol Chem (1995) 4.50
Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc Natl Acad Sci U S A (1994) 4.49
The chicken oestrogen receptor sequence: homology with v-erbA and the human oestrogen and glucocorticoid receptors. EMBO J (1986) 4.47
The signal recognition particle. Annu Rev Biochem (2001) 4.47
Isolation of nuclear pore complexes in association with a lamina. Proc Natl Acad Sci U S A (1975) 4.46
A protein-conducting channel in the endoplasmic reticulum. Cell (1991) 4.45
Gene gating: a hypothesis. Proc Natl Acad Sci U S A (1985) 4.45
A signal sequence for the insertion of a transmembrane glycoprotein. Similarities to the signals of secretory proteins in primary structure and function. J Biol Chem (1978) 4.43
Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J Cell Biol (1988) 4.42
Regulation of ribosome biogenesis by the rapamycin-sensitive TOR-signaling pathway in Saccharomyces cerevisiae. Mol Biol Cell (1999) 4.34
Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle. Nature (1989) 4.25
Tryptic dissection and reconstitution of translocation activity for nascent presecretory proteins across microsomal membranes. Proc Natl Acad Sci U S A (1979) 4.22
Nascent prehormones are intermediates in the biosynthesis of authentic bovine pituitary growth hormone and prolactin. Proc Natl Acad Sci U S A (1977) 4.14
Protein translocation across the endoplasmic reticulum. I. Detection in the microsomal membrane of a receptor for the signal recognition particle. J Cell Biol (1982) 4.10
Chicken ovalbumin contains an internal signal sequence. Nature (1979) 4.06
The two steps of nuclear import, targeting to the nuclear envelope and translocation through the nuclear pore, require different cytosolic factors. Cell (1992) 4.05
Immunoprecipitation of proteins from cell-free translations. Methods Enzymol (1983) 4.04
Isolation and characterization of a proteinaceous subnuclear fraction composed of nuclear matrix, peripheral lamina, and nuclear pore complexes from embryos of Drosophila melanogaster. J Cell Biol (1982) 4.03
A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm. Cell (1993) 3.98
Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway. Proc Natl Acad Sci U S A (1987) 3.95
Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex. Science (1997) 3.95
Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranes. J Biol Chem (1978) 3.80
Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p. J Biol Chem (1997) 3.76
Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins. Proc Natl Acad Sci U S A (1995) 3.73
Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science (1996) 3.70
A distinct nuclear import pathway used by ribosomal proteins. Cell (1997) 3.66
SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex. J Cell Biol (1998) 3.65
Biogenesis of peroxisomes: intracellular site of synthesis of catalase and uricase. Proc Natl Acad Sci U S A (1978) 3.63
Nopp140 shuttles on tracks between nucleolus and cytoplasm. Cell (1992) 3.63
Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis. J Cell Biol (1986) 3.62
A new family of yeast nuclear pore complex proteins. J Cell Biol (1992) 3.60
An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle. Science (1990) 3.55
Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle. Proc Natl Acad Sci U S A (1986) 3.55
Post-translational cleavage of presecretory proteins with an extract of rough microsomes from dog pancreas containing signal peptidase activity. Proc Natl Acad Sci U S A (1977) 3.54
Karyopherins and nuclear import. Curr Opin Struct Biol (2001) 3.53
Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes. Proc Natl Acad Sci U S A (1995) 3.52
Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants. Cell (1985) 3.50
Detection of prokaryotic signal peptidase in an Escherichia coli membrane fraction: endoproteolytic cleavage of nascent f1 pre-coat protein. Proc Natl Acad Sci U S A (1978) 3.48
NH2-terminal amino acid sequences of precursor and mature forms of the ribulose-1,5-bisphosphate carboxylase small subunit from Chlamydomonas reinhardtii. J Cell Biol (1979) 3.47