Published in J Biol Chem on November 10, 1981
Regulation of cell motility by mitogen-activated protein kinase. J Cell Biol (1997) 7.14
Engineering unnatural nucleotide specificity for Rous sarcoma virus tyrosine kinase to uniquely label its direct substrates. Proc Natl Acad Sci U S A (1997) 3.59
Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15. EMBO J (1993) 3.30
cdc2 phosphorylation is required for its interaction with cyclin. EMBO J (1991) 3.22
Wortmannin inactivates phosphoinositide 3-kinase by covalent modification of Lys-802, a residue involved in the phosphate transfer reaction. Mol Cell Biol (1996) 3.08
Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity. Proc Natl Acad Sci U S A (1987) 2.99
CAK, the p34cdc2 activating kinase, contains a protein identical or closely related to p40MO15. EMBO J (1993) 2.96
Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle. EMBO J (1995) 2.96
Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases. Proc Natl Acad Sci U S A (1986) 2.95
Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe. Mol Cell Biol (1986) 2.66
Functional expression and RNA binding analysis of the interferon-induced, double-stranded RNA-activated, 68,000-Mr protein kinase in a cell-free system. Mol Cell Biol (1991) 2.55
Mutational activation of c-raf-1 and definition of the minimal transforming sequence. Mol Cell Biol (1990) 2.24
Isolation of cDNA clones coding for the catalytic subunit of mouse cAMP-dependent protein kinase. Proc Natl Acad Sci U S A (1986) 2.20
Neither arginine nor histidine can carry out the function of lysine-295 in the ATP-binding site of p60src. Mol Cell Biol (1986) 2.15
A gene which encodes a predicted protein kinase can restore some functions of the ras gene in fission yeast. EMBO J (1988) 2.02
A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein delivery. EMBO J (1991) 1.93
Mechanisms of p34cdc2 regulation. Mol Cell Biol (1993) 1.89
Autoactivation of catalytic (C alpha) subunit of cyclic AMP-dependent protein kinase by phosphorylation of threonine 197. Mol Cell Biol (1993) 1.84
A lysine in the ATP-binding site of P130gag-fps is essential for protein-tyrosine kinase activity. EMBO J (1986) 1.77
Lysine residue 121 in the proposed ATP-binding site of the v-mos protein is required for transformation. Proc Natl Acad Sci U S A (1985) 1.74
Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning. Proc Natl Acad Sci U S A (1987) 1.63
Herpesviruses encode an unusual protein-serine/threonine kinase which is nonessential for growth in cultured cells. J Virol (1992) 1.53
Structural features that specify tyrosine kinase activity deduced from homology modeling of the epidermal growth factor receptor. Proc Natl Acad Sci U S A (1993) 1.53
Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II. Mol Cell Biol (1988) 1.48
Ras-dependent signal transduction is indispensable but not sufficient for the activation of AP1/Jun by PKC delta. EMBO J (1994) 1.30
Drosophila DBT lacking protein kinase activity produces long-period and arrhythmic circadian behavioral and molecular rhythms. Mol Cell Biol (2007) 1.26
The receptor kinase family: primary structure of rhodopsin kinase reveals similarities to the beta-adrenergic receptor kinase. Proc Natl Acad Sci U S A (1991) 1.25
A novel type of protein kinase phosphorylates actin in the actin-fragmin complex. EMBO J (1996) 1.18
Isolation of temperature-sensitive tyrosine kinase mutants of v-abl oncogene by screening with antibodies for phosphotyrosine. Proc Natl Acad Sci U S A (1987) 1.18
Autophosphorylation within the Atg1 activation loop is required for both kinase activity and the induction of autophagy in Saccharomyces cerevisiae. Genetics (2010) 1.17
Acetylation of conserved lysines in the catalytic core of cyclin-dependent kinase 9 inhibits kinase activity and regulates transcription. Mol Cell Biol (2008) 1.15
Nucleic acid sequence and oncogenic properties of the HZ2 feline sarcoma virus v-abl insert. J Virol (1987) 1.10
Interacting protein kinases involved in the regulation of flagellar length. Mol Biol Cell (2006) 1.10
A three-dimensional model of the Cdc2 protein kinase: localization of cyclin- and Suc1-binding regions and phosphorylation sites. Mol Cell Biol (1993) 1.08
Inhibition of Xenopus oocyte meiotic maturation by catalytically inactive protein kinase A. Proc Natl Acad Sci U S A (2002) 1.08
Phosphoryl transfer by protein kinase A is captured in a crystal lattice. J Am Chem Soc (2013) 1.01
Expression, purification and characterization of recombinant mitogen-activated protein kinase kinases. Biochem J (1994) 0.97
Evidence that rat liver pyruvate dehydrogenase kinase activator protein is a pyruvate dehydrogenase kinase. Biochem J (1991) 0.90
A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts. Skelet Muscle (2012) 0.88
Probing the peptide binding site of the cAMP-dependent protein kinase by using a peptide-based photoaffinity label. Proc Natl Acad Sci U S A (1988) 0.83
Affinity labelling of smooth-muscle myosin light-chain kinase with 5'-[p-(fluorosulphonyl)benzoyl]adenosine. Biochem J (1993) 0.81
Ascl1-induced neuronal differentiation of P19 cells requires expression of a specific inhibitor protein of cyclic AMP-dependent protein kinase. J Neurochem (2011) 0.77
Decoding the Interactions Regulating the Active State Mechanics of Eukaryotic Protein Kinases. PLoS Biol (2016) 0.75
Integration of signaling in the kinome: Architecture and regulation of the αC Helix. Biochim Biophys Acta (2015) 0.75
Covalent Modifiers: A Chemical Perspective on the Reactivity of α,β-Unsaturated Carbonyls with Thiols via Hetero-Michael Addition Reactions. J Med Chem (2016) 0.75
Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. Methods Enzymol (1983) 17.47
Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template. DNA (1984) 14.55
Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science (1991) 13.85
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. Nucleic Acids Res (1982) 13.52
Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science (1991) 9.99
Identification of a signal for rapid export of proteins from the nucleus. Cell (1995) 9.92
Fluorescence ratio imaging of cyclic AMP in single cells. Nature (1991) 4.45
Phosphorylation sites in enolase and lactate dehydrogenase utilized by tyrosine protein kinases in vivo and in vitro. J Biol Chem (1984) 4.20
Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions. J Biol Chem (1991) 3.93
Direct evidence that oncogenic tyrosine kinases and cyclic AMP-dependent protein kinase have homologous ATP-binding sites. Nature (1984) 3.91
Crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MgATP and peptide inhibitor. Biochemistry (1993) 3.88
Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering. Proc Natl Acad Sci U S A (2001) 3.77
Phosphorylation and inactivation of BAD by mitochondria-anchored protein kinase A. Mol Cell (1999) 3.46
Dynamics of cAMP-dependent protein kinase. Chem Rev (2001) 3.18
Regulatory subunit of protein kinase A: structure of deletion mutant with cAMP binding domains. Science (1995) 3.18
Portal and peripheral vein immunoreactive insulin concentrations before and after glucose infusion. Diabetes (1970) 3.12
A protein that activates expression of a multidrug efflux transporter upon binding the transporter substrates. J Biol Chem (1994) 3.08
Assay formats involving acridinium-ester-labeled DNA probes. Clin Chem (1989) 2.93
A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein. J Cell Biol (1999) 2.69
2.2 A refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MnATP and a peptide inhibitor. Acta Crystallogr D Biol Crystallogr (1993) 2.66
cAMP-dependent protein kinase: crystallographic insights into substrate recognition and phosphotransfer. Protein Sci (1994) 2.62
Protein N-myristoylation in Escherichia coli: reconstitution of a eukaryotic protein modification in bacteria. Proc Natl Acad Sci U S A (1990) 2.61
Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template. Methods Enzymol (1987) 2.59
Crystal structures of the myristylated catalytic subunit of cAMP-dependent protein kinase reveal open and closed conformations. Protein Sci (1993) 2.44
Structure of the Saccharomyces cerevisiae HO gene and analysis of its upstream regulatory region. Mol Cell Biol (1986) 2.36
A genetically encoded, fluorescent indicator for cyclic AMP in living cells. Nat Cell Biol (2000) 2.34
Primary structure of Torpedo californica acetylcholinesterase deduced from its cDNA sequence. Nature (1986) 2.32
Two highly similar multidrug transporters of Bacillus subtilis whose expression is differentially regulated. J Bacteriol (1995) 2.26
Mutagenesis of Fujinami sarcoma virus: evidence that tyrosine phosphorylation of P130gag-fps modulates its biological activity. Cell (1984) 2.18
Identification of Src, Fyn, Lyn, PI3K and Abl SH3 domain ligands using phage display libraries. EMBO J (1994) 2.14
Aneuploid colon cancer cells have a robust spindle checkpoint. EMBO Rep (2001) 2.12
Identification of a novel protein kinase A anchoring protein that binds both type I and type II regulatory subunits. J Biol Chem (1997) 2.10
Phosphorylation and activation of cAMP-dependent protein kinase by phosphoinositide-dependent protein kinase. Proc Natl Acad Sci U S A (1998) 2.09
High resolution analysis of functional determinants on human tissue-type plasminogen activator. J Biol Chem (1991) 2.02
Structural adaptations of lactate dehydrogenase isozymes. Proc Natl Acad Sci U S A (1977) 1.88
Structure-function relationships in lactate dehydrogenase. Proc Natl Acad Sci U S A (1973) 1.87
The protein kinase resource. Trends Biochem Sci (1997) 1.85
Expression of the catalytic subunit of cAMP-dependent protein kinase in Escherichia coli. J Biol Chem (1989) 1.85
Advantages of 2'-O-methyl oligoribonucleotide probes for detecting RNA targets. Nucleic Acids Res (1998) 1.82
Climate change and the integrity of science. Science (2010) 1.80
D-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domain. Proc Natl Acad Sci U S A (1997) 1.79
A lysine in the ATP-binding site of P130gag-fps is essential for protein-tyrosine kinase activity. EMBO J (1986) 1.77
Movement of the free catalytic subunit of cAMP-dependent protein kinase into and out of the nucleus can be explained by diffusion. Mol Biol Cell (1993) 1.71
A template for the protein kinase family. Trends Biochem Sci (1993) 1.69
Phage display selection of ligand residues important for Src homology 3 domain binding specificity. Proc Natl Acad Sci U S A (1995) 1.64
Energetic limits of phosphotransfer in the catalytic subunit of cAMP-dependent protein kinase as measured by viscosity experiments. Biochemistry (1992) 1.64
Molecular basis for regulatory subunit diversity in cAMP-dependent protein kinase: crystal structure of the type II beta regulatory subunit. Structure (2001) 1.59
Probing the cyclic nucleotide binding sites of cAMP-dependent protein kinases I and II with analogs of adenosine 3',5'-cyclic phosphorothioates. J Biol Chem (1990) 1.57
Role of the glycine triad in the ATP-binding site of cAMP-dependent protein kinase. J Biol Chem (1997) 1.55
Phosphorylation modulates catalytic function and regulation in the cAMP-dependent protein kinase. Biochemistry (1995) 1.55
Phosphorylation of histones 1 and 3 and nonhistone high mobility group 14 by an endogenous kinase in HeLa metaphase chromosomes. J Biol Chem (1982) 1.54
Structural features that specify tyrosine kinase activity deduced from homology modeling of the epidermal growth factor receptor. Proc Natl Acad Sci U S A (1993) 1.53
Expression of the catalytic subunit of cAMP-dependent protein kinase in Escherichia coli: multiple isozymes reflect different phosphorylation states. Protein Eng (1993) 1.52
A binary complex of the catalytic subunit of cAMP-dependent protein kinase and adenosine further defines conformational flexibility. Structure (1997) 1.51
Crystal structure of a polyhistidine-tagged recombinant catalytic subunit of cAMP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine. Biochemistry (1997) 1.50
Active site mutations define the pathway for the cooperative activation of cAMP-dependent protein kinase. Biochemistry (1996) 1.48
Domain movements in protein kinases. Curr Opin Struct Biol (1994) 1.47
NH2-Terminal targeting motifs direct dual specificity A-kinase-anchoring protein 1 (D-AKAP1) to either mitochondria or endoplasmic reticulum. J Cell Biol (1999) 1.46
Syk is activated by phosphotyrosine-containing peptides representing the tyrosine-based activation motifs of the high affinity receptor for IgE. J Biol Chem (1995) 1.44
Heat-stable inhibitors of cAMP-dependent protein kinase carry a nuclear export signal. J Biol Chem (1994) 1.43
The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain. Proc Natl Acad Sci U S A (1987) 1.42
N-myristylation of the catalytic subunit of cAMP-dependent protein kinase conveys structural stability. J Biol Chem (1993) 1.42
Profile of the disulfide bonds in acetylcholinesterase. J Biol Chem (1986) 1.42
Ribitol dehydrogenase from Klebsiella aerogenes. Purification and subunit structure. Biochem J (1974) 1.39
Serum protein electrophoresis in 147 dogs. Vet Rec (2011) 1.38
Systematic mutational analysis of cAMP-dependent protein kinase identifies unregulated catalytic subunits and defines regions important for the recognition of the regulatory subunit. J Biol Chem (1992) 1.37
The catalytic subunit of cAMP-dependent protein kinase: prototype for an extended network of communication. Prog Biophys Mol Biol (1999) 1.36
Structural comparisons of cAMP-dependent protein kinases I and II from porcine skeletal muscle. J Biol Chem (1979) 1.36
Dissecting cAMP binding domain A in the RIalpha subunit of cAMP-dependent protein kinase. Distinct subsites for recognition of cAMP and the catalytic subunit. J Biol Chem (1998) 1.34
Physiological inhibitors of the catalytic subunit of cAMP-dependent protein kinase: effect of MgATP on protein-protein interactions. Biochemistry (1993) 1.33
600 ps molecular dynamics reveals stable substructures and flexible hinge points in cAMP dependent protein kinase. Biopolymers (1999) 1.32
Structural basis of the intrasteric regulation of myosin light chain kinases. Science (1992) 1.31
Association of catalytic and regulatory subunits of cyclic AMP-dependent protein kinase requires a negatively charged side group at a conserved threonine. Mol Cell Biol (1990) 1.31
Dissection of the nucleotide and metal-phosphate binding sites in cAMP-dependent protein kinase. Biochemistry (1999) 1.29
Characterization of human blood coagulation factor XII cDNA. Prediction of the primary structure of factor XII and the tertiary structure of beta-factor XIIa. J Biol Chem (1985) 1.28
Identification of a partially rate-determining step in the catalytic mechanism of cAMP-dependent protein kinase: a transient kinetic study using stopped-flow fluorescence spectroscopy. Biochemistry (1997) 1.27
Phosphorylation of peptide substrates for the catalytic subunit of cAMP-dependent protein kinase. J Biol Chem (1993) 1.27
Signal transduction through the cAMP-dependent protein kinase. Mol Cell Biochem (1993) 1.27
Autophosphorylation of the catalytic subunit of cAMP-dependent protein kinase in Escherichia coli. Protein Eng (1997) 1.26
Interaction of p72syk with the gamma and beta subunits of the high-affinity receptor for immunoglobulin E, Fc epsilon RI. Mol Cell Biol (1995) 1.26
Dynamics of the distribution of cyclic AMP-dependent protein kinase in living cells. Proc Natl Acad Sci U S A (1990) 1.26
Identification of phosphorylation sites in the recombinant catalytic subunit of cAMP-dependent protein kinase. J Biol Chem (1993) 1.26
Expression in Escherichia coli of BCY1, the regulatory subunit of cyclic AMP-dependent protein kinase from Saccharomyces cerevisiae. Purification and characterization. J Biol Chem (1987) 1.24
Substrate specificities for yeast and mammalian cAMP-dependent protein kinases are similar but not identical. J Biol Chem (1991) 1.24
Structure of the mammalian catalytic subunit of cAMP-dependent protein kinase and an inhibitor peptide displays an open conformation. Acta Crystallogr D Biol Crystallogr (1993) 1.24
Portal and peripheral vein immunoreactive glucagon concentrations after arginine or glucose infusions. Diabetes (1974) 1.23
Mitogenic signaling by Ret/ptc2 requires association with enigma via a LIM domain. J Biol Chem (1996) 1.23
Serine-53 at the tip of the glycine-rich loop of cAMP-dependent protein kinase: role in catalysis, P-site specificity, and interaction with inhibitors. Biochemistry (2000) 1.23
Thermostable inhibitor of cAMP-dependent protein kinase enhances the rate of export of the kinase catalytic subunit from the nucleus. J Biol Chem (1994) 1.22
Correlation of the cAMP binding domain with a site of autophosphorylation on the regulatory subunit of cAMP-dependent protein kinase II from porcine skeletal muscle. J Biol Chem (1979) 1.21
Divalent metal ions influence catalysis and active-site accessibility in the cAMP-dependent protein kinase. Protein Sci (1993) 1.19
Identification of aspartate-184 as an essential residue in the catalytic subunit of cAMP-dependent protein kinase. Biochemistry (1988) 1.19
The double helix is dehydrated: evidence from the hydrolysis of acridinium ester-labeled probes. Biochemistry (1999) 1.19
Importance of the A-helix of the catalytic subunit of cAMP-dependent protein kinase for stability and for orienting subdomains at the cleft interface. Protein Sci (1997) 1.19
Kinetic analyses of mutations in the glycine-rich loop of cAMP-dependent protein kinase. Biochemistry (1998) 1.19
Mutagenesis of the regulatory subunit of yeast cAMP-dependent protein kinase. Isolation of site-directed mutants with altered binding affinity for catalytic subunit. J Biol Chem (1988) 1.19
A point mutation abolishes binding of cAMP to site A in the regulatory subunit of cAMP-dependent protein kinase. J Biol Chem (1988) 1.18
Mobilization of the A-kinase N-myristate through an isoform-specific intermolecular switch. Proc Natl Acad Sci U S A (1999) 1.18