Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

PubWeight™: 41.18‹?› | Rank: Top 0.01% | All-Time Top 1000

🔗 View Article (PMID 6997493)

Published in J Mol Biol on April 01, 1980


M J Casadaban, S N Cohen

Articles citing this

(truncated to the top 100)

In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol (1980) 19.04

Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. J Bacteriol (1984) 12.63

Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell (1992) 9.63

Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells. J Bacteriol (1993) 8.75

Synthesis of cholera toxin is positively regulated at the transcriptional level by toxR. Proc Natl Acad Sci U S A (1984) 8.47

Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res (1989) 7.78

Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc Natl Acad Sci U S A (1986) 7.43

Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin. Proc Natl Acad Sci U S A (1981) 7.24

Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl Environ Microbiol (1996) 7.12

Induction of Rhizobium meliloti nodC expression by plant exudate requires nodD. Proc Natl Acad Sci U S A (1985) 6.43

Engineering BioBrick vectors from BioBrick parts. J Biol Eng (2008) 5.98

Protein mobility in the cytoplasm of Escherichia coli. J Bacteriol (1999) 5.94

Construction of Tn5 lac, a transposon that fuses lacZ expression to exogenous promoters, and its introduction into Myxococcus xanthus. Proc Natl Acad Sci U S A (1984) 5.82

Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells. Proc Natl Acad Sci U S A (1984) 5.81

McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucleic Acids Res (1988) 5.76

The role of the delta-lysin gene (hld) in the regulation of virulence genes by the accessory gene regulator (agr) in Staphylococcus aureus. EMBO J (1990) 5.55

Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information. Mol Cell Biol (1988) 5.42

Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene. Proc Natl Acad Sci U S A (1983) 5.33

Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae. Mol Cell Biol (1990) 5.25

Excess information at bacteriophage T7 genomic promoters detected by a random cloning technique. Nucleic Acids Res (1989) 5.22

Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc Natl Acad Sci U S A (1997) 5.04

The Mycobacterium tuberculosis 65-kilodalton antigen is a heat shock protein which corresponds to common antigen and to the Escherichia coli GroEL protein. Infect Immun (1988) 5.03

The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region. Nucleic Acids Res (1984) 4.95

Molecular cloning, characterization, and complete nucleotide sequence of the gene for pneumolysin, the sulfhydryl-activated toxin of Streptococcus pneumoniae. Infect Immun (1987) 4.92

Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast. Proc Natl Acad Sci U S A (1981) 4.79

Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant. Mol Biol Cell (1996) 4.71

Type 1 fimbrial expression enhances Escherichia coli virulence for the urinary tract. Proc Natl Acad Sci U S A (1996) 4.69

From genetic footprinting to antimicrobial drug targets: examples in cofactor biosynthetic pathways. J Bacteriol (2002) 4.66

Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion. Proc Natl Acad Sci U S A (1985) 4.60

Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli. EMBO J (1986) 4.58

Conserved and variable functions of the sigmaE stress response in related genomes. PLoS Biol (2006) 4.46

Escherichia coli ribosomes translate in vivo with variable rate. EMBO J (1984) 4.39

The nodD gene of Rhizobium leguminosarum is autoregulatory and in the presence of plant exudate induces the nodA,B,C genes. EMBO J (1985) 4.39

Retroviral integrase domains: DNA binding and the recognition of LTR sequences. Nucleic Acids Res (1991) 4.39

Uses of lac fusions for the study of biological problems. Microbiol Rev (1985) 4.38

Dysfunctional MreB inhibits chromosome segregation in Escherichia coli. EMBO J (2003) 4.37

Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread. Infect Immun (1992) 4.35

Transposon-facilitated DNA sequencing. Proc Natl Acad Sci U S A (1991) 4.29

Isolation and characterization of Streptococcus cremoris Wg2-specific promoters. Appl Environ Microbiol (1987) 4.27

Complex glnA-glnL-glnG operon of Escherichia coli. J Bacteriol (1982) 4.25

Membrane protein sorting in the yeast secretory pathway: evidence that the vacuole may be the default compartment. J Cell Biol (1992) 4.20

Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast. J Cell Biol (1989) 4.13

RelE, a global inhibitor of translation, is activated during nutritional stress. Proc Natl Acad Sci U S A (2001) 4.10

The gene structure of human anti-haemophilic factor IX. EMBO J (1984) 4.09

The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal. J Cell Biol (1986) 4.07

Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells. Proc Natl Acad Sci U S A (1985) 3.95

Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product. Proc Natl Acad Sci U S A (1982) 3.89

Bacillus sporulation gene spo0H codes for sigma 30 (sigma H). J Bacteriol (1988) 3.88

Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA. Nucleic Acids Res (1981) 3.87

Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli. Proc Natl Acad Sci U S A (1980) 3.86

UmuD mutagenesis protein of Escherichia coli: overproduction, purification, and cleavage by RecA. Proc Natl Acad Sci U S A (1988) 3.85

Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proc Natl Acad Sci U S A (1981) 3.77

Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product. Mol Cell Biol (1988) 3.74

The MinD protein is a membrane ATPase required for the correct placement of the Escherichia coli division site. EMBO J (1991) 3.72

Synthetic curved DNA sequences can act as transcriptional activators in Escherichia coli. EMBO J (1989) 3.69

Roles of MinC and MinD in the site-specific septation block mediated by the MinCDE system of Escherichia coli. J Bacteriol (1992) 3.68

Activation of alpha-toxin translation in Staphylococcus aureus by the trans-encoded antisense RNA, RNAIII. EMBO J (1995) 3.64

Regulation of plasmid replication. Microbiol Rev (1984) 3.55

The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex. J Cell Biol (1989) 3.55

Multiple pathways for SOS-induced mutagenesis in Escherichia coli: an overexpression of dinB/dinP results in strongly enhancing mutagenesis in the absence of any exogenous treatment to damage DNA. Proc Natl Acad Sci U S A (1997) 3.53

Deletion mutations affecting autonomously replicating sequence ARS1 of Saccharomyces cerevisiae. Mol Cell Biol (1984) 3.53

Molecular characterization and protein analysis of the cap region, which is essential for encapsulation in Bacillus anthracis. J Bacteriol (1989) 3.38

ClpB is the Escherichia coli heat shock protein F84.1. J Bacteriol (1991) 3.38

Nitrogen fixation specific regulatory genes of Klebsiella pneumoniae and Rhizobium meliloti share homology with the general nitrogen regulatory gene ntrC of K. pneumoniae. Nucleic Acids Res (1985) 3.38

A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast. J Cell Biol (1988) 3.37

Cloning and genetic characterization of the Helicobacter pylori and Helicobacter mustelae flaB flagellin genes and construction of H. pylori flaA- and flaB-negative mutants by electroporation-mediated allelic exchange. J Bacteriol (1993) 3.34

Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase. Proc Natl Acad Sci U S A (1983) 3.33

Structure and expression of the mRNA for murine granulocyte-macrophage colony stimulating factor. EMBO J (1985) 3.30

Use of the Escherichia coli beta-glucuronidase (gusA) gene as a reporter gene for analyzing promoters in lactic acid bacteria. Appl Environ Microbiol (1994) 3.25

A DNA binding protein specific for an origin of replication of herpes simplex virus type 1. Proc Natl Acad Sci U S A (1986) 3.24

Functional analysis of promoters in the nisin gene cluster of Lactococcus lactis. J Bacteriol (1996) 3.23

Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase. Biochem J (1990) 3.20

Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903. Proc Natl Acad Sci U S A (1980) 3.19

Mutations in E coli cistrons affecting adhesion to human cells do not abolish Pap pili fiber formation. EMBO J (1984) 3.18

The mouse glucocorticoid receptor: mapping of functional domains by cloning, sequencing and expression of wild-type and mutant receptor proteins. EMBO J (1986) 3.17

chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100. J Bacteriol (1993) 3.17

Suppression of beta-1,3-glucanase transgene expression in homozygous plants. EMBO J (1992) 3.14

DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma(E)-dependent extracytoplasmic stress response. Genes Dev (2002) 3.13

In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage. Proc Natl Acad Sci U S A (1984) 3.06

Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. Proc Natl Acad Sci U S A (2002) 3.06

Expression of bacterial beta-galactosidase in animal cells. Mol Cell Biol (1982) 3.06

A separate editing exonuclease for DNA replication: the epsilon subunit of Escherichia coli DNA polymerase III holoenzyme. Proc Natl Acad Sci U S A (1984) 3.05

Cloning restriction fragments that promote expression of a gene in Bacillus subtilis. J Bacteriol (1981) 3.01

The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J Bacteriol (2008) 2.97

Binding of the Citrobacter freundii AmpR regulator to a single DNA site provides both autoregulation and activation of the inducible ampC beta-lactamase gene. J Bacteriol (1989) 2.95

The Salmonella selC locus contains a pathogenicity island mediating intramacrophage survival. EMBO J (1997) 2.95

A maturation protein is essential for production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope. J Bacteriol (1989) 2.89

Human fibronectin: molecular cloning evidence for two mRNA species differing by an internal segment coding for a structural domain. EMBO J (1984) 2.88

Two modes of control of pilA, the gene encoding type 1 pilin in Escherichia coli. J Bacteriol (1985) 2.85

Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili. J Bacteriol (1987) 2.83

Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens. Mol Cell Biol (1986) 2.81

Pseudomonas solanacearum genes controlling both pathogenicity on tomato and hypersensitivity on tobacco are clustered. J Bacteriol (1987) 2.79

Prokaryotic DNA segregation by an actin-like filament. EMBO J (2002) 2.78

Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis. Appl Environ Microbiol (1989) 2.78

Isolation of the gene encoding the Hin recombinational enhancer binding protein. Proc Natl Acad Sci U S A (1988) 2.76

The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl Environ Microbiol (1998) 2.75

Protein secretion in plant cells can occur via a default pathway. Plant Cell (1990) 2.74

Organization and nucleotide sequences of two lactococcal bacteriocin operons. Appl Environ Microbiol (1991) 2.72

Enhanced recovery and restriction mapping of DNA fragments cloned in a new lambda vector. Nucleic Acids Res (1988) 2.71

Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation. Infect Immun (1989) 2.70

Articles by these authors

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol (1978) 75.38

Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A (1972) 57.06

Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol (1976) 33.01

Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A (1973) 25.61

Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A (1979) 22.59

In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol (1980) 19.04

High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet (1979) 17.16

Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol (1974) 15.89

Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast. Methods Enzymol (1983) 14.92

Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Proc Natl Acad Sci U S A (1973) 13.45

Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. J Bacteriol (1984) 12.63

Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev (1976) 12.28

Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli. J Mol Biol (1970) 11.86

Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol (1973) 11.40

Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A (1974) 10.51

Multiple molecular species of circular R-factor DNA isolated from Escherichia coli. Nature (1969) 9.02

Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A (1975) 8.85

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. Nature (1978) 8.76

New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase. Gene (1983) 8.24

Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc Natl Acad Sci U S A (1975) 7.60

Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts. Proc Natl Acad Sci U S A (1983) 6.79

Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature (1979) 6.56

Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli. J Bacteriol (1986) 6.06

Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans. Mol Gen Genet (1982) 5.99

Regulation of the regulatory gene for the arabinose pathway, araC. J Mol Biol (1976) 5.97

Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature (1976) 5.73

Transposable genetic elements and plasmid evolution. Nature (1976) 5.58

Revised interpretation of the origin of the pSC101 plasmid. J Bacteriol (1977) 5.51

Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A (1974) 5.47

Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J Bacteriol (1975) 5.35

Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions. Proc Natl Acad Sci U S A (1975) 5.06

Nonchromosomal antibiotic resistance in bacteria. V. Isolation and characterization of R factor mutants exhibiting temperature-sensitive repression of fertility. J Bacteriol (1972) 4.85

Molecular and genetic studies of an R factor system consisting of independent transfer and drug resistance plasmids. J Bacteriol (1973) 4.81

Are chlamydial infections the most prevalent venereal disease? JAMA (1975) 4.79

Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast. Proc Natl Acad Sci U S A (1981) 4.79

Conversion of beta-galactosidase to a membrane-bound state by gene fusion. Proc Natl Acad Sci U S A (1976) 4.61

Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique. J Bacteriol (1983) 4.56

Mini-mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing. J Bacteriol (1986) 4.45

Dengue and chikungunya virus infection in man in Thailand, 1962-1964. I. Observations on hospitalized patients with hemorrhagic fever. Am J Trop Med Hyg (1969) 4.41

Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol Gen Genet (1978) 4.33

Observations related to pathogenesis of dengue hemorrhagic fever. IV. Relation of disease severity to antibody response and virus recovered. Yale J Biol Med (1970) 4.30

Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons. Proc Natl Acad Sci U S A (1974) 4.27

Structure and genomic organization of the mouse dihydrofolate reductase gene. Cell (1980) 4.25

3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate. Gene (1980) 4.22

Differential expression of photosynthesis genes in R. capsulata results from segmental differences in stability within the polycistronic rxcA transcript. Cell (1985) 4.15

Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 element. J Bacteriol (1977) 4.08

Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance. Cell (1980) 4.01

Isolation of catenated forms of R factor DNA from minicells. Nat New Biol (1971) 3.99

Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. J Bacteriol (1975) 3.88

Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proc Natl Acad Sci U S A (1981) 3.77

Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature (1975) 3.70

Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids. J Mol Biol (1976) 3.63

Cloning and endonuclease mapping of the hepatitis B viral genome. Nature (1979) 3.62

Letter: Potential biohazards of recombinant DNA molecules. Science (1974) 3.45

Penicillinase-producing Neisseria gonorrhoeae. Lancet (1976) 3.37

A sensitive radioimmunoassay for detecting products translated from cloned DNA fragments. Cell (1978) 3.30

Hemorrhagic fevers of Southeast Asia and South America: a comparative appraisal. Prog Med Virol (1967) 3.22

In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon. Proc Natl Acad Sci U S A (1984) 3.10

In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage. Proc Natl Acad Sci U S A (1984) 3.06

Growth-rate dependent regulation of mRNA stability in Escherichia coli. Nature (1984) 3.06

Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells. Cell (1996) 2.92

Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon. Mol Gen Genet (1979) 2.83

Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids. Cell (1975) 2.80

The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments. Cell (1986) 2.79

Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1. Proc Natl Acad Sci U S A (1970) 2.76

In vivo site-specific genetic recombination promoted by the EcoRI restriction endonuclease. Proc Natl Acad Sci U S A (1977) 2.74

Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces. Mol Gen Genet (1985) 2.71

Coryneform bacterial endocarditis: difficulties in diagnosis and treatment, presentation of three cases, and review of literature. Mayo Clin Proc (1977) 2.63

Expression of a preproinsulin-beta-galactosidase gene fusion in mammalian cells. Proc Natl Acad Sci U S A (1983) 2.57

The usefulness of preoperative laboratory screening. JAMA (1985) 2.55

An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability. Cell (1988) 2.53

Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays. Genes Dev (2001) 2.49

Shock associated with dengue infection. I. Clinical and physiologic manifestations of dengue hemorrhagic fever in Thailand, 1964. J Pediatr (1966) 2.45

A+U content rather than a particular nucleotide order determines the specificity of RNase E cleavage. J Biol Chem (1994) 2.39

Identification and characterization of a self-regulated repressor of translocation of the Tn3 element. Proc Natl Acad Sci U S A (1979) 2.37

Translocation specificity of the Tn3 element: characterization of sites of multiple insertions. Cell (1980) 2.31

Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1. J Bacteriol (1979) 2.29

Complete nucleotide sequence of the Streptomyces lividans plasmid pIJ101 and correlation of the sequence with genetic properties. J Bacteriol (1988) 2.29

The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo. Cell (1991) 2.27

RNA degradation in Escherichia coli regulated by 3' adenylation and 5' phosphorylation. Nature (1995) 2.26

Carotid endarterectomy--an evidence-based review: report of the Therapeutics and Technology Assessment Subcommittee of the American Academy of Neurology. Neurology (2005) 2.26

Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product. Nature (1980) 2.24

Effect of premature termination of translation on mRNA stability depends on the site of ribosome release. Proc Natl Acad Sci U S A (1987) 2.24

Prior antiplatelet use does not affect hemorrhage growth or outcome after ICH. Neurology (2009) 2.23

Bovine papilloma virus plasmids replicate randomly in mouse fibroblasts throughout S phase of the cell cycle. Cell (1987) 2.22

In vitro and in vivo manipulations of bacteriophage Mu DNA: cloning of Mu ends and construction of mini-Mu's carrying selectable markers. Gene (1981) 2.21

Genetic recombination through protoplast fusion in Streptomyces. Nature (1977) 2.14

Characterization of the structural gene and putative 5'-regulatory sequences for human proopiomelanocortin. Nature (1982) 2.04

The TSG101 tumor susceptibility gene is located in chromosome 11 band p15 and is mutated in human breast cancer. Cell (1997) 2.02

Construction and characterization of a novel two-plasmid system for accomplishing temperature-regulated, amplified expression of cloned adventitious genes in Escherichia coli. Gene (1981) 2.01

The Escherichia coli pcnB gene promotes adenylylation of antisense RNAI of ColE1-type plasmids in vivo and degradation of RNAI decay intermediates. Proc Natl Acad Sci U S A (1993) 1.99

Structural and functional analysis of the par region of the pSC 10 1 plasmid. Cell (1984) 1.98

Control of insulin gene expression in pancreatic beta-cells and in an insulin-producing cell line, RIN-5F cells. I. Effects of glucose and cyclic AMP on the transcription of insulin mRNA. J Biol Chem (1985) 1.95

A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces. Nature (1980) 1.95

Structural and functional analysis of transcriptional control of the Rhodobacter capsulatus puf operon. J Bacteriol (1989) 1.92

The N-terminal domain of the rne gene product has RNase E activity and is non-overlapping with the arginine-rich RNA-binding site. J Mol Biol (1996) 1.92