PubRank

F W Studier

Author PubWeight™ 424.38‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 1990 61.47
2 Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 1986 55.34
3 Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol 1977 33.84
4 Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A 1986 32.58
5 Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol 1973 30.17
6 Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J Mol Biol 1983 19.98
7 Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 1987 17.98
8 Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A 1984 13.68
9 Bacteriophage T7. Science 1972 13.19
10 Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proc Natl Acad Sci U S A 1986 8.59
11 The genetics and physiology of bacteriophage T7. Virology 1969 7.72
12 Utilization of bacteriophage T7 late promoters in recombinant plasmids during infection. J Mol Biol 1981 5.66
13 Structural genomics: beyond the human genome project. Nat Genet 1999 5.58
14 A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein. Cell 1986 5.53
15 Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4. J Mol Biol 1981 4.56
16 T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease 3. Proc Natl Acad Sci U S A 1973 4.34
17 Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA. Proc Natl Acad Sci U S A 1978 4.01
18 T7 early RNAs are generated by site-specific cleavages. Proc Natl Acad Sci U S A 1973 3.83
19 Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase. J Mol Biol 1984 3.79
20 Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host. J Mol Biol 1975 3.62
21 T7-directed protein synthesis. Virology 1969 3.32
22 Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein. Proc Natl Acad Sci U S A 1978 3.11
23 Genetic analysis of non-essential bacteriophage T7 genes. J Mol Biol 1973 2.88
24 Physical mapping of the early region of bacteriophage T7 DNA. J Mol Biol 1973 2.74
25 T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell 1987 2.66
26 Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. J Mol Biol 1991 2.66
27 Molecular substructure of a viral receptor-recognition protein. The gp17 tail-fiber of bacteriophage T7. J Mol Biol 1988 2.59
28 Genetic and physical mapping in the early region of bacteriophage T7 DNA. J Mol Biol 1979 2.52
29 Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli. J Bacteriol 1975 2.50
30 The structural gene for a T7 endonuclease essential for phage DNA synthesis. Proc Natl Acad Sci U S A 1970 2.40
31 Organization and expression of bacteriophage T7 DNA. Cold Spring Harb Symp Quant Biol 1983 2.35
32 Genetic and physical mapping of the late region of bacteriophage T7 DNA by use of cloned fragments of T7 DNA. J Mol Biol 1981 2.20
33 Effects of consecutive AGG codons on translation in Escherichia coli, demonstrated with a versatile codon test system. J Bacteriol 1993 2.05
34 Effects of the conformation of single-stranded DNA on renaturation and aggregation. J Mol Biol 1969 2.00
35 Effect of RNAase III, cleavage on translation of bacteriophage T7 messenger RNAs. J Mol Biol 1975 1.88
36 Gene 3 endonuclease of bacteriophage T7 resolves conformationally branched structures in double-stranded DNA. J Mol Biol 1987 1.87
37 DNA sequencing by primer walking with strings of contiguous hexamers. Science 1992 1.85
38 Conformational changes of single-stranded DNA. J Mol Biol 1969 1.84
39 Signal-mediated import of bacteriophage T7 RNA polymerase into the Saccharomyces cerevisiae nucleus and specific transcription of target genes. Mol Cell Biol 1990 1.84
40 Enzymes and sites of genetic recombination: studies with gene-3 endonuclease of phage T7 and with site-affinity mutants of phage lambda. Cold Spring Harb Symp Quant Biol 1984 1.84
41 SAMase gene of bacteriophage T3 is responsible for overcoming host restriction. J Virol 1976 1.82
42 The transcription termination site at the end of the early region of bacteriophage T7 DNA. Nucleic Acids Res 1980 1.81
43 Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli. J Mol Biol 1995 1.73
44 Targeting bacteriophage T7 RNA polymerase to the mammalian cell nucleus. Gene 1988 1.67
45 Relationships among different strains of T7 and among T7-related bacteriophages. Virology 1979 1.61
46 The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase. Proc Natl Acad Sci U S A 1994 1.58
47 Protein kinase induction in Escherichia coli by bacteriophage T7. Proc Natl Acad Sci U S A 1974 1.54
48 Large scale purification and biochemical characterization of T7 primase/helicase proteins. Evidence for homodimer and heterodimer formation. J Biol Chem 1992 1.52
49 Structural genomics of enzymes involved in sterol/isoprenoid biosynthesis. Proc Natl Acad Sci U S A 2001 1.46
50 Purification of the gene 0.3 protein of bacteriophage T7, an inhibitor of the DNA restriction system of Escherichia coli. J Biol Chem 1981 1.45
51 Biochemical analysis of mutant T7 primase/helicase proteins defective in DNA binding, nucleotide hydrolysis, and the coupling of hydrolysis with DNA unwinding. J Biol Chem 1996 1.44
52 Inhibition of the type I restriction-modification enzymes EcoB and EcoK by the gene 0.3 protein of bacteriophage T7. J Mol Biol 1985 1.42
53 Pausing and termination by bacteriophage T7 RNA polymerase. J Mol Biol 1998 1.32
54 Integration of two sets of T7 mutants. Virology 1969 1.32
55 Survey and mapping of restriction endonuclease cleavage sites in bacteriophage T7 DNA. J Mol Biol 1979 1.31
56 Entry of bacteriophage T7 DNA into the cell and escape from host restriction. J Bacteriol 1988 1.28
57 Mechanism of inhibition of bacteriophage T7 RNA polymerase by T7 lysozyme. J Mol Biol 1997 1.20
58 Creation of a T7 autogene. Cloning and expression of the gene for bacteriophage T7 RNA polymerase under control of its cognate promoter. J Mol Biol 1991 1.20
59 Processing transcription, and translation of bacteriophage T7 messenger RNAs. Brookhaven Symp Biol 1975 1.17
60 Amino acid sequence of the gene 0.3 protein of bacteriophage T7 and nucleotide sequence of its mRNA. J Biol Chem 1981 1.12
61 Mutant bacteriophage T7 RNA polymerases with altered termination properties. J Mol Biol 1997 1.11
62 Expression of the bisphosphatase domain of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli. Proc Natl Acad Sci U S A 1988 1.10
63 Assembly of T7 capsids from independently expressed and purified head protein and scaffolding protein. J Mol Biol 1996 1.06
64 Identification and mapping of five new genes in bacteriophage T7. J Mol Biol 1981 1.01
65 T7 RNA polymerase directed expression of the Escherichia coli rrnB operon. EMBO J 1986 0.98
66 Ligation of hexamers on hexamer templates to produce primers for cycle sequencing or the polymerase chain reaction. Anal Biochem 1995 0.98
67 Cloning and expression of gene 4 of bacteriophage T7 and creation and analysis of T7 mutants lacking the 4A primase/helicase or the 4B helicase. J Biol Chem 1992 0.96
68 Cloning of the T7 genome in Escherichia coli: use of recombination between cloned sequences and bacteriophage T7 to identify genes involved in recombination and a clone containing the origin of T7 DNA replication. Cold Spring Harb Symp Quant Biol 1979 0.94
69 Resolution of branched DNA substrates by T7 endonuclease I and its inhibition. J Biol Chem 1991 0.93
70 T7 RNA polymerase can direct expression of influenza virus cap-binding protein (PB2) in Escherichia coli. Gene 1987 0.92
71 Isolation of transcriptionally active mutants of T7 RNA polymerase that do not support phage growth. J Mol Biol 1995 0.90
72 Polyacrylamide solutions for DNA sequencing by capillary electrophoresis: mesh sizes, separation and dispersion. Electrophoresis 1996 0.89
73 Structure of a yeast hypothetical protein selected by a structural genomics approach. Acta Crystallogr D Biol Crystallogr 2002 0.88
74 Expression of human factor IX and its subfragments in Escherichia coli and generation of antibodies to the subfragments. Biochemistry 1987 0.87
75 Selection, identification, and genetic analysis of random mutants in the cloned primase/helicase gene of bacteriophage T7. J Biol Chem 1996 0.85
76 Purification and characterization of T7 head-tail connectors expressed from the cloned gene. J Mol Biol 1996 0.84
77 Stringent control in Escherichia coli applies also to transcription by T7 RNA polymerase. J Biol Chem 1987 0.82
78 Characterization of a bimobile DNA junction. J Biol Chem 1990 0.79
79 Formation of concentration gradient and its application to DNA capillary electrophoresis. Electrophoresis 2000 0.78
80 Intrinsic viscosity of native and single-stranded T7 DNA and its relationship to sedimentation coefficient. Biopolymers 1969 0.75
81 Multi-capillary optical waveguides for DNA sequencing. Electrophoresis 1998 0.75