Published in EMBO J on February 01, 1993
The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition particle pathway. J Bacteriol (2003) 1.80
Anionic phospholipids are determinants of membrane protein topology. EMBO J (1997) 1.77
Oxa1p, an essential component of the N-tail protein export machinery in mitochondria. Proc Natl Acad Sci U S A (1998) 1.76
Membrane topology and insertion of membrane proteins: search for topogenic signals. Microbiol Mol Biol Rev (2000) 1.74
Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli. Proc Natl Acad Sci U S A (1998) 1.62
Transmembrane orientation of signal-anchor proteins is affected by the folding state but not the size of the N-terminal domain. EMBO J (1995) 1.58
Membrane protein topology: effects of delta mu H+ on the translocation of charged residues explain the 'positive inside' rule. EMBO J (1994) 1.53
Insertion into the mitochondrial inner membrane of a polytopic protein, the nuclear-encoded Oxa1p. EMBO J (1997) 1.53
Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner. EMBO Rep (2001) 1.25
Conservative sorting of F0-ATPase subunit 9: export from matrix requires delta pH across inner membrane and matrix ATP. EMBO J (1995) 1.22
Sec-independent translocation of a 100-residue periplasmic N-terminal tail in the E. coli inner membrane protein proW. EMBO J (1994) 1.21
Translocation of N-terminal tails across the plasma membrane. EMBO J (1994) 1.18
Biotinylation in vivo as a sensitive indicator of protein secretion and membrane protein insertion. J Bacteriol (1996) 1.16
Signal recognition particle-dependent inner membrane targeting of the PulG Pseudopilin component of a type II secretion system. J Bacteriol (2006) 1.12
Membrane protein biogenesis: the exception explains the rules. Proc Natl Acad Sci U S A (1998) 1.01
Effects of SecE depletion on the inner and outer membrane proteomes of Escherichia coli. J Bacteriol (2008) 0.94
Localization and integration of thylakoid protein translocase subunit cpTatC. Plant J (2009) 0.93
Plastids contain a second sec translocase system with essential functions. Plant Physiol (2010) 0.92
Competitive binding of the SecA ATPase and ribosomes to the SecYEG translocon. J Biol Chem (2012) 0.90
Characterizing folding, structure, molecular interactions and ligand gated activation of single sodium/proton antiporters. Naunyn Schmiedebergs Arch Pharmacol (2006) 0.84
Folding and activity of circularly permuted forms of a polytopic membrane protein. Proc Natl Acad Sci U S A (2000) 0.83
YidC protein, a molecular chaperone for LacY protein folding via the SecYEG protein machinery. J Biol Chem (2013) 0.81
Polarity and charge of the periplasmic loop determine the YidC and sec translocase requirement for the M13 procoat lep protein. J Biol Chem (2013) 0.80
The ribosome and YidC. New insights into the biogenesis of Escherichia coli inner membrane proteins. EMBO Rep (2003) 0.80
MifM monitors total YidC activities of Bacillus subtilis, including that of YidC2, the target of regulation. J Bacteriol (2014) 0.79
SecA cotranslationally interacts with nascent substrate proteins in vivo. J Bacteriol (2016) 0.77
SecA drives transmembrane insertion of RodZ, an unusual single-span membrane protein. J Mol Biol (2014) 0.77
Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A (1985) 68.48
Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. J Mol Biol (1992) 11.97
The spontaneous insertion of proteins into and across membranes: the helical hairpin hypothesis. Cell (1981) 7.77
The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology. EMBO J (1986) 5.48
Topogenic signals in integral membrane proteins. Eur J Biochem (1988) 5.16
Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues. Nature (1989) 4.45
Transcending the impenetrable: how proteins come to terms with membranes. Biochim Biophys Acta (1988) 4.08
The ATPase activity of SecA is regulated by acidic phospholipids, SecY, and the leader and mature domains of precursor proteins. Cell (1990) 4.00
The enzymology of protein translocation across the Escherichia coli plasma membrane. Annu Rev Biochem (1991) 3.85
Genetic analysis of protein export in Escherichia coli. Annu Rev Genet (1990) 3.73
The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane. Cell (1990) 3.58
Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope. J Biol Chem (1983) 3.22
Delta mu H+ and ATP function at different steps of the catalytic cycle of preprotein translocase. Cell (1991) 2.98
Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery. Proc Natl Acad Sci U S A (1990) 2.90
High-level expression of M13 gene II protein from an inducible polycistronic messenger RNA. Gene (1985) 2.56
Fine-tuning the topology of a polytopic membrane protein: role of positively and negatively charged amino acids. Cell (1990) 2.24
Positively charged amino acid residues can act as topogenic determinants in membrane proteins. Proc Natl Acad Sci U S A (1989) 1.86
Characterization of cold-sensitive secY mutants of Escherichia coli. J Bacteriol (1990) 1.63
Bacterial leader peptidase, a membrane protein without a leader peptide, uses the same export pathway as pre-secretory proteins. Cell (1984) 1.56
Membrane proteins: from sequence to structure. Protein Eng (1990) 1.54
Positively charged residues are important determinants of membrane protein topology. Trends Biochem Sci (1990) 1.52
Suppressor analysis suggests a multistep, cyclic mechanism for protein secretion in Escherichia coli. EMBO J (1992) 1.46
A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli. Proc Natl Acad Sci U S A (1991) 1.43
Distinct domains of an oligotopic membrane protein are Sec-dependent and Sec-independent for membrane insertion. J Biol Chem (1992) 1.37
Export of Escherichia coli alkaline phosphatase attached to an integral membrane protein, SecY. J Biol Chem (1989) 1.35
The cytoplasmic domain of Escherichia coli leader peptidase is a "translocation poison" sequence. Proc Natl Acad Sci U S A (1988) 1.34
The role of the polar, carboxyl-terminal domain of Escherichia coli leader peptidase in its translocation across the plasma membrane. J Biol Chem (1986) 1.30
A small hydrophobic domain anchors leader peptidase to the cytoplasmic membrane of Escherichia coli. J Biol Chem (1987) 1.29
The Tsr chemosensory transducer of Escherichia coli assembles into the cytoplasmic membrane via a SecA-dependent process. J Biol Chem (1988) 1.29
Leader peptidase. Mol Microbiol (1991) 1.20
Leader peptidase of Escherichia coli: critical role of a small domain in membrane assembly. Science (1987) 1.19
Accumulation of prolipoprotein in Escherichia coli mutants defective in protein secretion. J Bacteriol (1985) 1.14
Secretion of methanol-insoluble heat-stable enterotoxin (STB): energy- and secA-dependent conversion of pre-STB to an intermediate indistinguishable from the extracellular toxin. J Bacteriol (1990) 1.11
Different positively charged amino acids have similar effects on the topology of a polytopic transmembrane protein in Escherichia coli. J Biol Chem (1992) 1.10
Mapping of catalytically important domains in Escherichia coli leader peptidase. EMBO J (1990) 1.08
Alterations in the extracellular domain of M13 procoat protein make its membrane insertion dependent on secA and secY. Eur J Biochem (1988) 1.08
Membrane insertion of the Escherichia coli MalF protein in cells with impaired secretion machinery. J Biol Chem (1991) 1.07
SecB-independent export of Escherichia coli ribose-binding protein (RBP): some comparisons with export of maltose-binding protein (MBP) and studies with RBP-MBP hybrid proteins. J Bacteriol (1990) 1.05
Efficient translocation of positively charged residues of M13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion. EMBO J (1990) 1.04
Positive charges in the cytoplasmic domain of Escherichia coli leader peptidase prevent an apolar domain from functioning as a signal. EMBO J (1989) 0.97
Both a short hydrophobic domain and a carboxyl-terminal hydrophilic region are important for signal function in the Escherichia coli leader peptidase. J Biol Chem (1989) 0.97
Hydrophobic content and lipid interactions of wild-type and mutant OmpA signal peptides correlate with their in vivo function. Biochemistry (1991) 0.95
Export of honeybee prepromelittin in Escherichia coli depends on the membrane potential but does not depend on proteins secA and secY. J Biol Chem (1989) 0.91
In vitro translocation of secretory proteins possessing no charges at the mature domain takes place efficiently in a protonmotive force-dependent manner. J Biol Chem (1992) 0.90
A lower size limit exists for export of fragments of an outer membrane protein (OmpA) of Escherichia coli K-12. J Mol Biol (1989) 0.87
Temperature-dependent insertion of prolipoprotein into Escherichia coli membrane vesicles and requirements for ATP, soluble factors, and functional SecY protein for the overall translocation process. J Bacteriol (1989) 0.83
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol (2001) 66.87
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng (1997) 38.38
A new method for predicting signal sequence cleavage sites. Nucleic Acids Res (1986) 37.19
Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem (1983) 26.68
Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol (2000) 22.77
Signal sequences. The limits of variation. J Mol Biol (1985) 21.24
A hidden Markov model for predicting transmembrane helices in protein sequences. Proc Int Conf Intell Syst Mol Biol (1998) 14.18
How signal sequences maintain cleavage specificity. J Mol Biol (1984) 11.03
TopPred II: an improved software for membrane protein structure predictions. Comput Appl Biosci (1994) 10.03
ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci (1999) 9.82
Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method. Protein Eng (1997) 8.25
Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Sci (1998) 7.88
A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Int J Neural Syst (1999) 7.40
Mitochondrial targeting sequences may form amphiphilic helices. EMBO J (1986) 7.11
Machine learning approaches for the prediction of signal peptides and other protein sorting signals. Protein Eng (1999) 6.72
Sequence determinants of cytosolic N-terminal protein processing. Eur J Biochem (1986) 5.59
A conserved cleavage-site motif in chloroplast transit peptides. FEBS Lett (1990) 4.25
Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering. Protein Eng (1990) 4.17
How proteins adapt to a membrane-water interface. Trends Biochem Sci (2000) 3.76
Trans-membrane translocation of proteins. The direct transfer model. Eur J Biochem (1979) 3.13
Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane. J Biol Chem (1993) 3.02
Predicting the topology of eukaryotic membrane proteins. Eur J Biochem (1993) 2.73
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase. EMBO J (2000) 2.70
Membrane proteins: the amino acid composition of membrane-penetrating segments. Eur J Biochem (1981) 2.52
Analysis of the distribution of charged residues in the N-terminal region of signal sequences: implications for protein export in prokaryotic and eukaryotic cells. EMBO J (1984) 2.43
Cleavage-site motifs in mitochondrial targeting peptides. Protein Eng (1990) 2.38
The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J (1998) 2.35
A receptor component of the chloroplast protein translocation machinery. Science (1994) 2.34
Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett (2001) 2.28
Fine-tuning the topology of a polytopic membrane protein: role of positively and negatively charged amino acids. Cell (1990) 2.24
On the hydrophobic nature of signal sequences. Eur J Biochem (1981) 2.01
Competition between Sec- and TAT-dependent protein translocation in Escherichia coli. EMBO J (1999) 1.95
Prediction of organellar targeting signals. Biochim Biophys Acta (2001) 1.93
Structures of N-terminally acetylated proteins. Eur J Biochem (1985) 1.92
Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle. FEBS Lett (1996) 1.89
Topology, subcellular localization, and sequence diversity of the Mlo family in plants. J Biol Chem (1999) 1.88
Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor. Mol Microbiol (1997) 1.85
Net N-C charge imbalance may be important for signal sequence function in bacteria. J Mol Biol (1986) 1.85
Topological rules for membrane protein assembly in eukaryotic cells. J Biol Chem (1997) 1.83
Amino acid distributions around O-linked glycosylation sites. Biochem J (1991) 1.82
Anionic phospholipids are determinants of membrane protein topology. EMBO J (1997) 1.77
Molecular mechanism of membrane protein integration into the endoplasmic reticulum. Cell (1997) 1.70
Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli. Proc Natl Acad Sci U S A (1998) 1.62
Topological "frustration" in multispanning E. coli inner membrane proteins. Cell (1994) 1.59
Consensus predictions of membrane protein topology. FEBS Lett (2000) 1.55
Membrane protein topology: effects of delta mu H+ on the translocation of charged residues explain the 'positive inside' rule. EMBO J (1994) 1.53
Translation rate modification by preferential codon usage: intragenic position effects. J Theor Biol (1987) 1.51
A nascent secretory protein may traverse the ribosome/endoplasmic reticulum translocase complex as an extended chain. J Biol Chem (1996) 1.51
The aromatic residues Trp and Phe have different effects on the positioning of a transmembrane helix in the microsomal membrane. Biochemistry (1999) 1.49
Towards a comparative anatomy of N-terminal topogenic protein sequences. J Mol Biol (1986) 1.46
Chloroplast transit peptides from the green alga Chlamydomonas reinhardtii share features with both mitochondrial and higher plant chloroplast presequences. FEBS Lett (1990) 1.45
A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli. Proc Natl Acad Sci U S A (1991) 1.43
The distribution of charged amino acids in mitochondrial inner-membrane proteins suggests different modes of membrane integration for nuclearly and mitochondrially encoded proteins. Eur J Biochem (1992) 1.42
Feature-extraction from endopeptidase cleavage sites in mitochondrial targeting peptides. Proteins (1998) 1.41
The 'positive-inside rule' applies to thylakoid membrane proteins. FEBS Lett (1991) 1.40
The COOH-terminal ends of internal signal and signal-anchor sequences are positioned differently in the ER translocase. J Cell Biol (1994) 1.39
Architecture of helix bundle membrane proteins: an analysis of cytochrome c oxidase from bovine mitochondria. Protein Sci (1997) 1.38
Determination of the border between the transmembrane and cytoplasmic domains of human integrin subunits. J Biol Chem (1999) 1.37
A turn propensity scale for transmembrane helices. J Mol Biol (1999) 1.37
Signal sequences are not uniformly hydrophobic. J Mol Biol (1982) 1.37
Turns in transmembrane helices: determination of the minimal length of a "helical hairpin" and derivation of a fine-grained turn propensity scale. J Mol Biol (1999) 1.36
Life and death of a signal peptide. Nature (1998) 1.35
Stop-transfer function of pseudo-random amino acid segments during translocation across prokaryotic and eukaryotic membranes. Eur J Biochem (1998) 1.34
Different conformations of nascent polypeptides during translocation across the ER membrane. BMC Cell Biol (2000) 1.34
Defining a similarity threshold for a functional protein sequence pattern: the signal peptide cleavage site. Proteins (1996) 1.31
Positively and negatively charged residues have different effects on the position in the membrane of a model transmembrane helix. J Mol Biol (1998) 1.27
Proline-induced disruption of a transmembrane alpha-helix in its natural environment. J Mol Biol (1998) 1.26
A 12-residue-long polyleucine tail is sufficient to anchor synaptobrevin to the endoplasmic reticulum membrane. J Biol Chem (1996) 1.25
Properties of N-terminal tails in G-protein coupled receptors: a statistical study. Protein Eng (1995) 1.25
Glycosylation efficiency of Asn-Xaa-Thr sequons depends both on the distance from the C terminus and on the presence of a downstream transmembrane segment. J Biol Chem (2000) 1.25
Forced transmembrane orientation of hydrophilic polypeptide segments in multispanning membrane proteins. Mol Cell (1998) 1.25
Why mitochondria need a genome. FEBS Lett (1986) 1.23
Divergent evolution of membrane protein topology: the Escherichia coli RnfA and RnfE homologues. Proc Natl Acad Sci U S A (1999) 1.22
Sec-independent translocation of a 100-residue periplasmic N-terminal tail in the E. coli inner membrane protein proW. EMBO J (1994) 1.21
Prediction of N-terminal protein sorting signals. Curr Opin Struct Biol (1997) 1.18
Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: a rapid way to map helix-helix interactions in integral membrane proteins. Protein Sci (1996) 1.15
Membrane topology of the 60-kDa Oxa1p homologue from Escherichia coli. J Biol Chem (1998) 1.14
Positively charged amino acids placed next to a signal sequence block protein translocation more efficiently in Escherichia coli than in mammalian microsomes. Mol Gen Genet (1993) 1.13
Different positively charged amino acids have similar effects on the topology of a polytopic transmembrane protein in Escherichia coli. J Biol Chem (1992) 1.10
Mapping of catalytically important domains in Escherichia coli leader peptidase. EMBO J (1990) 1.08
Homology to region X from staphylococcal protein A is not unique to cell surface proteins. J Theor Biol (1987) 1.07
Phosphatidylethanolamine mediates insertion of the catalytic domain of leader peptidase in membranes. FEBS Lett (1998) 1.04
Three-dimensional model for the membrane domain of Escherichia coli leader peptidase based on disulfide mapping. Biochemistry (1993) 1.02
Analysis and prediction of mitochondrial targeting peptides. Methods Cell Biol (2001) 1.02
Do protein-lipid interactions determine the recognition of transmembrane helices at the ER translocon? Biochem Soc Trans (2005) 1.01
Distant downstream sequence determinants can control N-tail translocation during protein insertion into the endoplasmic reticulum membrane. J Biol Chem (2000) 1.01
Architecture of beta-barrel membrane proteins: analysis of trimeric porins. Protein Sci (1998) 1.00
A signal peptide with a proline next to the cleavage site inhibits leader peptidase when present in a sec-independent protein. FEBS Lett (1992) 1.00
Trans-membrane translocation of proteins. A detailed physico-chemical analysis. Eur J Biochem (1980) 1.00
De novo design of integral membrane proteins. Nat Struct Biol (1994) 0.99
Directionality in protein translocation across membranes: the N-tail phenomenon. Trends Cell Biol (1995) 0.98
Inhibition of protein translocation across the endoplasmic reticulum membrane by sterols. J Biol Chem (2001) 0.95
Membrane topology of Kch, a putative K+ channel from Escherichia coli. J Biol Chem (1996) 0.94
The internal repeats in the Na+/Ca2+ exchanger-related Escherichia coli protein YrbG have opposite membrane topologies. J Biol Chem (2001) 0.93
Breaking the camel's back: proline-induced turns in a model transmembrane helix. J Mol Biol (1998) 0.93
Alanine insertion scanning mutagenesis of lactose permease transmembrane helices. J Biol Chem (1997) 0.92
In vitro membrane integration of leader peptidase depends on the Sec machinery and anionic phospholipids and can occur post-translationally. FEBS Lett (1997) 0.91
Helical sidedness and the distribution of polar residues in trans-membrane helices. J Mol Biol (1983) 0.91
A de novo designed signal peptide cleavage cassette functions in vivo. J Biol Chem (1991) 0.91
Mitochondrial targeting sequences. Why 'non-amphiphilic' peptides may still be amphiphilic. FEBS Lett (1988) 0.91