15N NMR relaxation studies of free and inhibitor-bound 4-oxalocrotonate tautomerase: backbone dynamics and entropy changes of an enzyme upon inhibitor binding.

PubWeight™: 1.09‹?› | Rank: Top 10%

🔗 View Article (PMID 8973173)

Published in Biochemistry on December 17, 1996

Authors

J T Stivers1, C Abeygunawardana, A S Mildvan

Author Affiliations

1: Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2185, USA.

Articles citing this

Binding sites in Escherichia coli dihydrofolate reductase communicate by modulating the conformational ensemble. Proc Natl Acad Sci U S A (2000) 2.47

FAST-Modelfree: a program for rapid automated analysis of solution NMR spin-relaxation data. J Biomol NMR (2003) 2.28

Configurational entropy in protein-peptide binding: computational study of Tsg101 ubiquitin E2 variant domain with an HIV-derived PTAP nonapeptide. J Mol Biol (2009) 1.26

Intrinsic disorder in protein interactions: insights from a comprehensive structural analysis. PLoS Comput Biol (2009) 1.25

Functional dynamics in the active site of the ribonuclease binase. Proc Natl Acad Sci U S A (2001) 1.23

trans-3-Chloroacrylic acid dehalogenase from Pseudomonas pavonaceae 170 shares structural and mechanistic similarities with 4-oxalocrotonate tautomerase. J Bacteriol (2001) 1.19

Anisotropic rotational diffusion in model-free analysis for a ternary DHFR complex. J Biomol NMR (2001) 1.12

The role of backbone conformational heat capacity in protein stability: temperature dependent dynamics of the B1 domain of Streptococcal protein G. Protein Sci (2000) 1.04

Propagation of experimental uncertainties using the Lipari-Szabo model-free analysis of protein dynamics. J Biomol NMR (1998) 1.03

ComSin: database of protein structures in bound (complex) and unbound (single) states in relation to their intrinsic disorder. Nucleic Acids Res (2009) 1.02

Temperature-dependent spectral density analysis applied to monitoring backbone dynamics of major urinary protein-I complexed with the pheromone 2- sec-butyl-4,5-dihydrothiazole. J Biomol NMR (2004) 0.95

Solution NMR and computational methods for understanding protein allostery. J Phys Chem B (2013) 0.93

Ligand-induced changes in dynamics in the RT loop of the C-terminal SH3 domain of Sem-5 indicate cooperative conformational coupling. Protein Sci (2003) 0.88

An Analysis of MIF Structural Features that Control Functional Activation of CD74. Chem Biol (2015) 0.86

Dynamics of stromelysin/inhibitor interactions studied by 15N NMR relaxation measurements: comparison of ligand binding to the S1-S3 and S'1-S'3 subsites. J Biomol NMR (1999) 0.85

Analysis of the internal motion of free and ligand-bound human lysozyme by use of 15N NMR relaxation measurement: a comparison with those of hen lysozyme. Protein Sci (2000) 0.83

Critical role of substrate conformational change in the proton transfer process catalyzed by 4-oxalocrotonate tautomerase. J Am Chem Soc (2009) 0.83

Backbone dynamics of a bacterially expressed peptide from the receptor binding domain of Pseudomonas aeruginosa pilin strain PAK from heteronuclear 1H-15N NMR spectroscopy. J Biomol NMR (2000) 0.82

Backbone dynamics of free barnase and its complex with barstar determined by 15N NMR relaxation study. J Biomol NMR (2000) 0.80

Backbone dynamics of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue, by 15N NMR relaxation methods. J Biomol NMR (2006) 0.80

Backbone dynamics of a symmetric calmodulin dimer in complex with the calmodulin-binding domain of the basic-helix-loop-helix transcription factor SEF2-1/E2-2: a highly dynamic complex. Biophys J (2005) 0.79

Ligand-induced changes in the structure and dynamics of Escherichia coli peptide deformylase. Biochemistry (2009) 0.79

Effect of hydrophobic core packing on sidechain dynamics. J Biomol NMR (1999) 0.79

Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate. Protein Sci (2000) 0.77

Using NMR spectroscopy to elucidate the role of molecular motions in enzyme function. Prog Nucl Magn Reson Spectrosc (2015) 0.76

NMR backbone dynamics of VEK-30 bound to the human plasminogen kringle 2 domain. Biophys J (2010) 0.75

Articles by these authors

ATP-binding site of adenylate kinase: mechanistic implications of its homology with ras-encoded p21, F1-ATPase, and other nucleotide-binding proteins. Proc Natl Acad Sci U S A (1986) 5.55

Improved sensitivity of HSQC spectra of exchanging protons at short interscan delays using a new fast HSQC (FHSQC) detection scheme that avoids water saturation. J Magn Reson B (1995) 4.93

Deoxynucleoside [1-thio]triphosphates prevent proofreading during in vitro DNA synthesis. Proc Natl Acad Sci U S A (1981) 4.14

Immunochemical properties of the staphylococcal poly-N-acetylglucosamine surface polysaccharide. Infect Immun (2002) 3.14

Spinach leaf phosphoenolpyruvate carboxylase: purification, properties, and kinetic studies. Arch Biochem Biophys (1974) 2.46

Zinc in DNA polymerases. Biochem Biophys Res Commun (1971) 2.15

Aspects of enzyme mechanisms studies by nuclear spin relazation induced by paramagnetic probes. Adv Enzymol Relat Areas Mol Biol (1970) 1.90

On the fidelity of DNA replication: manganese mutagenesis in vitro. Biochemistry (1985) 1.90

Kinetic and ultraviolet spectroscopic studies of active-site mutants of delta 5-3-ketosteroid isomerase. Biochemistry (1989) 1.85

Purification and determination of the structure of capsular polysaccharide of Vibrio vulnificus M06-24. J Bacteriol (1992) 1.81

Kinetic and magnetic resonance studies of the pyruvate kinase reaction. II. Complexes of enzyme, metal, and substrates. J Biol Chem (1966) 1.79

Structure and dynamics of a denatured 131-residue fragment of staphylococcal nuclease: a heteronuclear NMR study. Biochemistry (1994) 1.55

Environments and mechanistic roles of the tyrosine residues of delta 5-3-ketosteroid isomerase. Biochemistry (1993) 1.53

Chemoprotective properties of phenylpropenoids, bis(benzylidene)cycloalkanones, and related Michael reaction acceptors: correlation of potencies as phase 2 enzyme inducers and radical scavengers. J Med Chem (1998) 1.53

Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins. Infect Immun (1997) 1.49

Kinetic and magnetic resonance studies of active-site mutants of staphylococcal nuclease: factors contributing to catalysis. Biochemistry (1987) 1.46

NMR docking of a substrate into the X-ray structure of staphylococcal nuclease. Proteins (1992) 1.46

Lectin recognition of host-like saccharide motifs in streptococcal cell wall polysaccharides. Glycobiology (1995) 1.46

Vaccinia DNA topoisomerase I: single-turnover and steady-state kinetic analysis of the DNA strand cleavage and ligation reactions. Biochemistry (1994) 1.46

Vaccinia DNA topoisomerase I: evidence supporting a free rotation mechanism for DNA supercoil relaxation. Biochemistry (1997) 1.35

NMR evidence for the participation of a low-barrier hydrogen bond in the mechanism of delta 5-3-ketosteroid isomerase. Proc Natl Acad Sci U S A (1996) 1.33

The role of metal ions in the mechanisms of DNA and RNA polymerases. CRC Crit Rev Biochem (1979) 1.33

Stuies on the interaction of substrate and monovalent and divalent cations with pyruvate kinase. Biochemistry (1966) 1.33

Mechanism of malic enzyme from pigeon liver. Magnetic resonance and kinetic studies of the role of Mn2+. J Biol Chem (1976) 1.32

Proton magnetic relaxation studies of the interaction of D-xylose and xylitol with D-xylose isomerase. Characterization of metal-enzyme-substrate interactions. J Biol Chem (1975) 1.27

Kinetic analysis of Escherichia coli deoxyribonucleic acid polymerase I. J Biol Chem (1975) 1.27

The mechanism of aconitase action. I. Preparation, physical properties of the enzyme, and activation by iron (II). J Biol Chem (1971) 1.25

Escherichia coli deoxyribonucleic acid polymerase I, a zinc metalloenzyme. Nuclear quadrupolar relaxation studies of the role of bound zinc. J Biol Chem (1973) 1.24

4-Oxalocrotonate tautomerase: pH dependence of catalysis and pKa values of active site residues. Biochemistry (1996) 1.24

Kinetic and magnetic resonance studies of effects of genetic substitution of a Ca2+-liganding amino acid in staphylococcal nuclease. Biochemistry (1986) 1.24

Conformation of deoxynucleoside triphosphate substrates on DNA polymerase I from Escherichia coli as determined by nuclear magnetic relaxation. J Biol Chem (1975) 1.23

The role of Glu 57 in the mechanism of the Escherichia coli MutT enzyme by mutagenesis and heteronuclear NMR. Biochemistry (1996) 1.21

Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies. Biochemistry (1996) 1.20

The mechanism of Escherichia coli deoxyribonucleic acid polymerase I. Magnetic resonance and kinetic studies of the role of metals. J Biol Chem (1972) 1.17

Solution structure of the MutT enzyme, a nucleoside triphosphate pyrophosphohydrolase. Biochemistry (1995) 1.17

Structures of enzyme-bound metal-nucleotide complexes in the phosphoryl transfer reaction of muscle pyruvate kinase. 31P NMR studies with magnesium and kinetic studies with chromium nucleotides. J Biol Chem (1977) 1.15

Escherichia coli orf17 codes for a nucleoside triphosphate pyrophosphohydrolase member of the MutT family of proteins. Cloning, purification, and characterization of the enzyme. J Biol Chem (1996) 1.13

Kinetic and magnetic resonance studies of the mechanism of D-xylose isomerase. I. Binary and ternary complexes with manganese(II), substrates, and inhibitors. J Biol Chem (1972) 1.12

Electron paramagnetic resonance, 1-H, and 13C nuclear magnetic resonance studies of the interaction of manganese and bicarbonate with ribulose 1, 5-diphosphate carboxylase. J Biol Chem (1974) 1.12

Combined effects of two mutations of catalytic residues on the ketosteroid isomerase reaction. Biochemistry (1990) 1.09

Catalytic mechanism of an active-site mutant (D38N) of delta 5-3-ketosteroid isomerase. Direct spectroscopic evidence for dienol intermediates. Biochemistry (1991) 1.08

Studies of the mechanism of the delta 5-3-ketosteroid isomerase reaction by substrate, solvent, and combined kinetic deuterium isotope effects on wild-type and mutant enzymes. Biochemistry (1990) 1.08

Hydrogen bonding at the active site of delta 5-3-ketosteroid isomerase. Biochemistry (1997) 1.08

Nuclear relaxation measurements of the geometry of enzyme-bound substrates and analogs. Methods Enzymol (1978) 1.07

NMR studies of conformations and interactions of substrates and ribonucleotide templates bound to the large fragment of DNA polymerase I. Biochemistry (1986) 1.07

Water exchange filter with improved sensitivity (WEX II) to study solvent-exchangeable protons. Application to the consensus zinc finger peptide CP-1. J Magn Reson B (1996) 1.07

Solution structure of Delta 5-3-ketosteroid isomerase complexed with the steroid 19-nortestosterone hemisuccinate. Biochemistry (1998) 1.06

Vaccinia DNA topoisomerase I: kinetic evidence for general acid-base catalysis and a conformational step. Biochemistry (1994) 1.06

Comprehensive chromatographic and spectroscopic methods for the separation and identification of intact glucosinolates. Anal Biochem (1996) 1.05

Nuclear magnetic resonance studies of the function of potassium in the mechanism of pyruvate kinase. Biochemistry (1972) 1.05

High-precision measurement of hydrogen bond lengths in proteins by nuclear magnetic resonance methods. Proteins (1999) 1.05

NMR studies of the role of hydrogen bonding in the mechanism of triosephosphate isomerase. Biochemistry (1997) 1.05

NMR studies of the MgATP binding site of adenylate kinase and of a 45-residue peptide fragment of the enzyme. Biochemistry (1985) 1.04

NMR structure of a stable "OB-fold" sub-domain isolated from staphylococcal nuclease. J Mol Biol (1995) 1.04

Mutational, kinetic, and NMR studies of the roles of conserved glutamate residues and of lysine-39 in the mechanism of the MutT pyrophosphohydrolase. Biochemistry (2000) 1.03

Proton transfer in the mechanism of triosephosphate isomerase. Biochemistry (1998) 1.02

The enzymatic carboxylation of phosphoenolpyruvate. IV. The binding of manganese and substrates by phosphoenolpyruvate carbosy-kinase and phosphoenolypyruvate carboxylase. J Biol Chem (1968) 1.02

Magnetic resonance and kinetic studies of the manganese(II) ion and substrate complexes of the catalytic subunit of adenosine 3',5'-monophosphate dependent protein kinase from bovine heart. Biochemistry (1979) 1.02

Studies of the catalytic mechanism of an active-site mutant (Y14F) of delta 5-3-ketosteroid isomerase by kinetic deuterium isotope effects. Biochemistry (1991) 1.02

Positioning of a spin-labeled substrate analogue into the structure of delta 5-3-ketosteroid isomerase by combined kinetic, magnetic resonance, and X-ray diffraction methods. Biochemistry (1987) 1.02

Kinetic, stereochemical, and structural effects of mutations of the active site arginine residues in 4-oxalocrotonate tautomerase. Biochemistry (1999) 1.01

Nuclear Overhauser effect studies of the conformations and binding site environments of deoxynucleoside triphosphate substrates bound to DNA polymerase I and its large fragment. Biochemistry (1985) 1.01

Dual divalent cation requirement of the MutT dGTPase. Kinetic and magnetic resonance studies of the metal and substrate complexes. J Biol Chem (1994) 1.01

The cell wall polysaccharide of Streptococcus gordonii 38: structure and immunochemical comparison with the receptor polysaccharides of Streptococcus oralis 34 and Streptococcus mitis J22. Glycobiology (1994) 1.01

Pyruvate carboxylase. VII. A possible role for tightly bound manganese. J Biol Chem (1966) 1.00

Solution structure of the quaternary MutT-M2+-AMPCPP-M2+ complex and mechanism of its pyrophosphohydrolase action. Biochemistry (1997) 0.99

Arrangement and conformations of substrates at the active site of pyruvate kinase from model building studies based on magnetic resonance data. J Biol Chem (1976) 0.99

Ultraviolet spectroscopic evidence for decreased motion of the active site tyrosine residue of delta 5-3-ketosteroid isomerase by steroid binding. Biochemistry (1995) 0.99

Magnetic resonance studies of the anomeric distribution and manganese binding properties of fructose phosphates. Biochem Biophys Res Commun (1972) 0.98

Magnetic resonance measurements of intersubstrate distances at the active site of protein kinase using substitution-inert cobalt(III) and chromium(III) complexes of adenosine 5'-(beta, gamma-methylenetriphosphate). Biochemistry (1980) 0.98

Electron and nuclear magnetic resonance studies of the interaction of pyruvate with transcarboxylase. Biochemistry (1974) 0.97

Stereochemistry of the concerted enolization catalyzed by delta 5-3-ketosteroid isomerase. Biochemistry (1991) 0.97

Computer simulation studies of mammalian pyruvate kinase. J Biol Chem (1967) 0.97

Effects of mutations of the active site arginine residues in 4-oxalocrotonate tautomerase on the pKa values of active site residues and on the pH dependence of catalysis. Biochemistry (1999) 0.97

Magnetic resonance studies of the conformation of enzyme-bound adenylyl(3' leads to 5')uridine and adenosine 5'-triphosphate on RNA polymerase from Esherichia coli. Biochemistry (1977) 0.97

Electron spin resonance studies with dioldehydrase. Evidence for radical intermediates in reactions catalyzed by coenzyme B 12. J Biol Chem (1973) 0.97

Role of magnesium and other divalent cations in ATP-utilizing enzymes. Magnesium (1987) 0.97

The structural basis for the perturbed pKa of the catalytic base in 4-oxalocrotonate tautomerase: kinetic and structural effects of mutations of Phe-50. Biochemistry (2001) 0.97

NMR studies of the backbone protons and secondary structure of pentapeptide and heptapeptide substrates bound to bovine heart protein kinase. Biochemistry (1984) 0.96

Nuclear relaxation and kinetic studies of the role of Mn 2+ in the mechanism of enolase. Biochemistry (1973) 0.96

Magnetic resonance studies of the role of the divalent cation in the mechanism of yeast aldolase. Biochemistry (1971) 0.95

Mechanism of enzyme action. Annu Rev Biochem (1974) 0.95

13C NMR relaxation studies of backbone and side chain motion of the catalytic tyrosine residue in free and steroid-bound delta 5-3-ketosteroid isomerase. Biochemistry (1996) 0.95

Mechanistic studies of cAMP-dependent protein kinase action. CRC Crit Rev Biochem (1984) 0.95

Fluorocitrate inhibition of aconitase: relative configuration of inhibitory isomer by x-ray crystallography. Science (1970) 0.94

Pyruvate carboxylase. X. The demonstration of direct coordination of pyruvate and alpha-ketobutyrate by the bound manganese and the formation of enzyme-metal-substrate bridge complexes. Biochemistry (1967) 0.94

Magnetic resonance studies of the proximity and spatial arrangement of propionyl coenzyme A and pyruvate on a biotin-metalloenzyme, transcarboxylase. Biochemistry (1976) 0.94