Mutations associated with pyrazinamide resistance in pncA of Mycobacterium tuberculosis complex organisms.

PubWeight™: 3.46‹?› | Rank: Top 1%

🔗 View Article (PMC 163764)

Published in Antimicrob Agents Chemother on March 01, 1997

Authors

S Sreevatsan1, X Pan, Y Zhang, B N Kreiswirth, J M Musser

Author Affiliations

1: Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA.

Articles citing this

Genome-wide analysis of synonymous single nucleotide polymorphisms in Mycobacterium tuberculosis complex organisms: resolution of genetic relationships among closely related microbial strains. Genetics (2002) 4.11

Genome analysis of multi- and extensively-drug-resistant tuberculosis from KwaZulu-Natal, South Africa. PLoS One (2009) 3.68

Multidrug-resistant Mycobacterium tuberculosis: molecular perspectives. Emerg Infect Dis (1998) 3.05

Whole-genome sequencing for prediction of Mycobacterium tuberculosis drug susceptibility and resistance: a retrospective cohort study. Lancet Infect Dis (2015) 2.92

Differentiation of clinical Mycobacterium tuberculosis complex isolates by gyrB DNA sequence polymorphism analysis. J Clin Microbiol (2000) 2.77

Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays. J Clin Microbiol (1999) 2.65

Differentiation among members of the Mycobacterium tuberculosis complex by molecular and biochemical features: evidence for two pyrazinamide-susceptible subtypes of M. bovis. J Clin Microbiol (2000) 2.64

Evaluation of genotype MTBC assay for differentiation of clinical Mycobacterium tuberculosis complex isolates. J Clin Microbiol (2003) 2.21

Phenotypic characterization of pncA mutants of Mycobacterium tuberculosis. Antimicrob Agents Chemother (2000) 2.15

pncA mutations as a major mechanism of pyrazinamide resistance in Mycobacterium tuberculosis: spread of a monoresistant strain in Quebec, Canada. Antimicrob Agents Chemother (2000) 2.10

Characterization of new mutations in pyrazinamide-resistant strains of Mycobacterium tuberculosis and identification of conserved regions important for the catalytic activity of the pyrazinamidase PncA. Antimicrob Agents Chemother (1999) 2.09

Testing of susceptibility of Mycobacterium tuberculosis to pyrazinamide with the nonradiometric BACTEC MGIT 960 system. J Clin Microbiol (2002) 1.98

Phenotypic and genotypic characterization of Mycobacterium africanum isolates from West Africa. J Clin Microbiol (1999) 1.74

A balancing act: efflux/influx in mycobacterial drug resistance. Antimicrob Agents Chemother (2009) 1.74

Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis. Antimicrob Agents Chemother (1999) 1.69

Allele-specific PCR method based on pncA and oxyR sequences for distinguishing Mycobacterium bovis from Mycobacterium tuberculosis: intraspecific M. bovis pncA sequence polymorphism. J Clin Microbiol (1998) 1.66

Pyrazinamide susceptibility testing in Mycobacterium tuberculosis: a systematic review with meta-analyses. Antimicrob Agents Chemother (2011) 1.62

Genetic methods for assessing antimicrobial resistance. Antimicrob Agents Chemother (1999) 1.60

The non-clonality of drug resistance in Beijing-genotype isolates of Mycobacterium tuberculosis from the Western Cape of South Africa. BMC Genomics (2010) 1.54

pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis isolates from northwestern Russia. Antimicrob Agents Chemother (1999) 1.53

Effects of pyrazinamide on fatty acid synthesis by whole mycobacterial cells and purified fatty acid synthase I. J Bacteriol (2002) 1.52

Comparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing with Mycobacterium tuberculosis. J Clin Microbiol (2000) 1.51

Pyrazinamide resistance and pncA gene mutations in Mycobacterium tuberculosis. Antimicrob Agents Chemother (2008) 1.37

Purification, gene cloning, targeted knockout, overexpression, and biochemical characterization of the major pyrazinamidase from Mycobacterium smegmatis. J Bacteriol (1998) 1.20

pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis isolates in Portugal. Antimicrob Agents Chemother (2004) 1.17

Comparative evaluation of Löwenstein-Jensen proportion method, BacT/ALERT 3D system, and enzymatic pyrazinamidase assay for pyrazinamide susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol (2006) 1.16

In-depth molecular characterization of Mycobacterium tuberculosis from New Delhi--predominance of drug resistant isolates of the 'modern' (TbD1) type. PLoS One (2009) 1.16

Importance of the genetic diversity within the Mycobacterium tuberculosis complex for the development of novel antibiotics and diagnostic tests of drug resistance. Antimicrob Agents Chemother (2012) 1.16

Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculosis drug pyrazinamide. Antimicrob Agents Chemother (1999) 1.16

Surveillance of pyrazinamide susceptibility among multidrug-resistant Mycobacterium tuberculosis isolates from Siriraj Hospital, Thailand. BMC Microbiol (2010) 1.14

Elucidating emergence and transmission of multidrug-resistant tuberculosis in treatment experienced patients by whole genome sequencing. PLoS One (2013) 1.13

Systematic analysis of pyrazinamide-resistant spontaneous mutants and clinical isolates of Mycobacterium tuberculosis. Antimicrob Agents Chemother (2012) 1.13

Study of the structure-activity relationships for the pyrazinamidase (PncA) from Mycobacterium tuberculosis. Biochem J (2001) 1.10

EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology (2008) 1.06

Widespread pyrazinamide-resistant Mycobacterium tuberculosis family in a low-incidence setting. J Clin Microbiol (2003) 1.06

pncA mutations in clinical Mycobacterium tuberculosis isolates from Korea. BMC Infect Dis (2001) 1.06

Temperature-mediated heteroduplex analysis performed by using denaturing high-performance liquid chromatography to identify sequence polymorphisms in Mycobacterium tuberculosis complex organisms. J Clin Microbiol (2002) 1.04

Genetics and pulmonary medicine. 5. Genetics of drug resistant tuberculosis. Thorax (1998) 0.99

Characterization of pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis in Brazil. Antimicrob Agents Chemother (2005) 0.96

Evaluation of the invader assay, a linear signal amplification method, for identification of mutations associated with resistance to rifampin and isoniazid in Mycobacterium tuberculosis. Antimicrob Agents Chemother (2000) 0.94

Detection by denaturing gradient gel electrophoresis of pncA mutations associated with pyrazinamide resistance in Mycobacterium tuberculosis isolates from the United States-Mexico border region. Antimicrob Agents Chemother (2005) 0.94

Systematic review of mutations in pyrazinamidase associated with pyrazinamide resistance in Mycobacterium tuberculosis clinical isolates. Antimicrob Agents Chemother (2015) 0.94

Peruvian and globally reported amino acid substitutions on the Mycobacterium tuberculosis pyrazinamidase suggest a conserved pattern of mutations associated to pyrazinamide resistance. Infect Genet Evol (2009) 0.93

Pyrazinamide susceptibility testing of Mycobacterium tuberculosis by high resolution melt analysis. Tuberculosis (Edinb) (2013) 0.91

pncA gene expression and prediction factors on pyrazinamide resistance in Mycobacterium tuberculosis. Tuberculosis (Edinb) (2013) 0.90

Current issues on molecular and immunological diagnosis of tuberculosis. Yonsei Med J (2007) 0.89

'Z(S)-MDR-TB' versus 'Z(R)-MDR-TB': improving treatment of MDR-TB by identifying pyrazinamide susceptibility. Emerg Microbes Infect (2012) 0.88

Insight to pyrazinamide resistance in Mycobacterium tuberculosis by molecular docking. Bioinformation (2009) 0.88

Rapid identification of mycobacteria and drug-resistant Mycobacterium tuberculosis by use of a single multiplex PCR and DNA sequencing. J Clin Microbiol (2011) 0.88

Evaluation of colorimetric methods using nicotinamide for rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis. J Clin Microbiol (2010) 0.87

Spectrum of pncA mutations in multidrug-resistant Mycobacterium tuberculosis isolates obtained in Latvia. Antimicrob Agents Chemother (2004) 0.86

Molecular epidemiological study of pyrazinamide-resistance in clinical isolates of mycobacterium tuberculosis from South India. Int J Mol Sci (2010) 0.85

Mycobacterium smegmatis has two pyrazinamidase enzymes, PncA and pzaA. J Bacteriol (2000) 0.85

Mechanisms of Pyrazinamide Action and Resistance. Microbiol Spectr (2013) 0.83

Association between pncA gene mutations, pyrazinamidase activity, and pyrazinamide susceptibility testing in Mycobacterium tuberculosis. Antimicrob Agents Chemother (2014) 0.80

Rapid detection of Mycobacterium tuberculosis and pyrazinamide susceptibility related to pncA mutations in sputum specimens through an integrated gene-to-protein function approach. J Clin Microbiol (2013) 0.79

Outbreak of pyrazinamide-monoresistant tuberculosis identified using genotype cluster and social media analysis. Int J Tuberc Lung Dis (2014) 0.78

Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae. Biochemistry (2011) 0.78

Mechanistic analysis elucidating the relationship between Lys96 mutation in Mycobacterium tuberculosis pyrazinamidase enzyme and pyrazinamide susceptibility. BMC Genomics (2015) 0.77

Epidemiologic Correlates of Pyrazinamide-Resistant Mycobacterium tuberculosis in New York City. Antimicrob Agents Chemother (2015) 0.77

Multicenter evaluation of a nonweekend reading schedule for radiometric pyrazinamide susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol (2002) 0.77

Simple fibroblast-based assay to test the pyrazinamide susceptibility of Mycobacterium tuberculosis. Antimicrob Agents Chemother (2005) 0.75

Genotypic characterization of drug resistant Mycobacterium tuberculosis in Quebec, 2002-2012. BMC Microbiol (2016) 0.75

Clinical implication of novel drug resistance-conferring mutations in resistant tuberculosis. Eur J Clin Microbiol Infect Dis (2017) 0.75

Articles cited by this

Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol (1993) 45.22

Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol (1987) 15.79

Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family. JAMA (1996) 9.45

Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus. Nat Med (1996) 8.94

Antimicrobial agent resistance in mycobacteria: molecular genetic insights. Clin Microbiol Rev (1995) 7.55

Simple pyrazinamidase and urease tests for routine identification of mycobacteria. Am Rev Respir Dis (1974) 7.40

Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas. J Clin Microbiol (1994) 6.91

Amplification of a species-specific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis. J Clin Microbiol (1991) 5.99

Characterization of the catalase-peroxidase gene (katG) and inhA locus in isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis by automated DNA sequencing: restricted array of mutations associated with drug resistance. J Infect Dis (1996) 5.91

Pyrazinamide susceptibility and amidase activity of tubercle bacilli. Am Rev Respir Dis (1967) 5.45

Genetic diversity and relationships among Streptococcus pyogenes strains expressing serotype M1 protein: recent intercontinental spread of a subclone causing episodes of invasive disease. Infect Immun (1995) 4.40

Rapid Mycobacterium species assignment and unambiguous identification of mutations associated with antimicrobial resistance in Mycobacterium tuberculosis by automated DNA sequencing. Arch Pathol Lab Med (1995) 4.10

Is Mycobacterium tuberculosis 15,000 years old? J Infect Dis (1994) 3.97

Relationship between rifampin MICs for and rpoB mutations of Mycobacterium tuberculosis strains isolated in Japan. Antimicrob Agents Chemother (1996) 3.82

Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis. J Clin Microbiol (1996) 3.70

The action of antituberculosis drugs in short-course chemotherapy. Tubercle (1985) 3.69

Pyrazinamide (aldinamide) in the treatment of pulmonary tuberculosis. Am Rev Tuberc (1952) 2.98

Determination of pyrazinamide MICs for Mycobacterium tuberculosis at different pHs by the radiometric method. Antimicrob Agents Chemother (1988) 2.87

Comparative antimycobacterial activities of rifampin, rifapentine, and KRM-1648 against a collection of rifampin-resistant Mycobacterium tuberculosis isolates with known rpoB mutations. Antimicrob Agents Chemother (1996) 2.75

Improved method for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide. J Clin Microbiol (1982) 2.44

Phase variation of gonococcal protein II: regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence. Cell (1989) 2.42

Pyrazinamide sterilizing activity in vitro against semidormant Mycobacterium tuberculosis bacterial populations. Am Rev Respir Dis (1992) 2.34

Fluoroquinolone resistance associated with specific gyrase mutations in clinical isolates of multidrug-resistant Mycobacterium tuberculosis. J Infect Dis (1996) 2.22

Mutation position and type of substitution in the beta-subunit of the RNA polymerase influence in-vitro activity of rifamycins in rifampicin-resistant Mycobacterium tuberculosis. J Antimicrob Chemother (1995) 2.22

Characterization of streptomycin resistance mechanisms among Mycobacterium tuberculosis isolates from patients in New York City. Antimicrob Agents Chemother (1996) 1.92

Drug-resistant tuberculosis: inconsistent results of pyrazinamide susceptibility testing. JAMA (1995) 1.83

Observations in vitro on the suitability of pyrazinamide for intermittent chemotherapy of tuberculosis. Tubercle (1970) 1.41

Activity of n-propyl pyrazinoate against pyrazinamide-resistant Mycobacterium tuberculosis: investigations into mechanism of action of and mechanism of resistance to pyrazinamide. Antimicrob Agents Chemother (1995) 1.38

Articles by these authors

Electric field effect in atomically thin carbon films. Science (2004) 45.10

Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol (1986) 24.31

The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature (1983) 19.44

Regulated translation initiation controls stress-induced gene expression in mammalian cells. Mol Cell (2000) 17.44

Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase. Nature (1999) 16.97

Restricted structural gene polymorphism in the Mycobacterium tuberculosis complex indicates evolutionarily recent global dissemination. Proc Natl Acad Sci U S A (1997) 15.25

IntAct--open source resource for molecular interaction data. Nucleic Acids Res (2006) 13.82

Coupling of stress in the ER to activation of JNK protein kinases by transmembrane protein kinase IRE1. Science (2000) 13.62

Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains. J Clin Microbiol (1999) 11.77

Comparison of methods based on different molecular epidemiological markers for typing of Mycobacterium tuberculosis complex strains: interlaboratory study of discriminatory power and reproducibility. J Clin Microbiol (1999) 11.63

Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response. Nat Cell Biol (2000) 11.62

Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuber Lung Dis (1998) 11.56

Osteoarthritis: new insights. Part 1: the disease and its risk factors. Ann Intern Med (2000) 11.12

Perk is essential for translational regulation and cell survival during the unfolded protein response. Mol Cell (2000) 11.09

Prognostic factors analysis of 17,600 melanoma patients: validation of the American Joint Committee on Cancer melanoma staging system. J Clin Oncol (2001) 10.20

Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family. JAMA (1996) 9.45

Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus. Nat Med (1996) 8.94

Smoking in China: findings of the 1996 National Prevalence Survey. JAMA (1999) 8.67

Diabetes mellitus and exocrine pancreatic dysfunction in perk-/- mice reveals a role for translational control in secretory cell survival. Mol Cell (2001) 8.60

Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat Med (1995) 8.51

Pulmonary expression of interleukin-13 causes inflammation, mucus hypersecretion, subepithelial fibrosis, physiologic abnormalities, and eotaxin production. J Clin Invest (1999) 8.38

Mammalian sweet taste receptors. Cell (2001) 8.04

Disruption of IRS-2 causes type 2 diabetes in mice. Nature (1998) 7.94

A small-molecule nitroimidazopyran drug candidate for the treatment of tuberculosis. Nature (2000) 7.66

The effects of specific medical conditions on the functional limitations of elders in the Framingham Study. Am J Public Health (1994) 7.38

Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas. J Clin Microbiol (1994) 6.91

Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Res (1999) 6.85

Intercontinental spread of a multiresistant clone of serotype 23F Streptococcus pneumoniae. J Infect Dis (1991) 6.69

Significant acidification in major Chinese croplands. Science (2010) 6.68

Evolutionary genetic relationships of clones of Salmonella serovars that cause human typhoid and other enteric fevers. Infect Immun (1990) 6.42

TFAR19, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal. Biochem Biophys Res Commun (1999) 6.33

Frequency of specific cancer types in dermatomyositis and polymyositis: a population-based study. Lancet (2001) 6.23

Evolutionary genomics of Staphylococcus aureus: insights into the origin of methicillin-resistant strains and the toxic shock syndrome epidemic. Proc Natl Acad Sci U S A (2001) 6.22

Disruption of p53 in human cancer cells alters the responses to therapeutic agents. J Clin Invest (1999) 6.13

Cloning of mammalian Ire1 reveals diversity in the ER stress responses. EMBO J (1998) 5.96

Genetic relationships of serologically nontypable and serotype b strains of Haemophilus influenzae. Infect Immun (1986) 5.92

Characterization of the catalase-peroxidase gene (katG) and inhA locus in isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis by automated DNA sequencing: restricted array of mutations associated with drug resistance. J Infect Dis (1996) 5.91

An update on the epidemiology of knee and hip osteoarthritis with a view to prevention. Arthritis Rheum (1998) 5.72

MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. Nat Genet (1999) 5.61

Population genetics of pathogenic bacteria. Microb Pathog (1987) 5.59

Signal transduction cascades regulating fungal development and virulence. Microbiol Mol Biol Rev (2000) 5.49

Horizontal transfer of multiple penicillin-binding protein genes, and capsular biosynthetic genes, in natural populations of Streptococcus pneumoniae. Mol Microbiol (1991) 5.45

Smads: transcriptional activators of TGF-beta responses. Cell (1998) 5.33

Identification of a W variant outbreak of Mycobacterium tuberculosis via population-based molecular epidemiology. JAMA (1999) 5.25

Methylation of histone H4 at arginine 3 facilitating transcriptional activation by nuclear hormone receptor. Science (2001) 5.25

Virulence of a Mycobacterium tuberculosis clinical isolate in mice is determined by failure to induce Th1 type immunity and is associated with induction of IFN-alpha /beta. Proc Natl Acad Sci U S A (2001) 5.21

Weight loss reduces the risk for symptomatic knee osteoarthritis in women. The Framingham Study. Ann Intern Med (1992) 5.05

Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase. Mol Cell (2001) 5.04

Regulation of the p85/p110 phosphatidylinositol 3'-kinase: stabilization and inhibition of the p110alpha catalytic subunit by the p85 regulatory subunit. Mol Cell Biol (1998) 5.01

Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease. Proc Natl Acad Sci U S A (1989) 5.00

Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology. Proc Natl Acad Sci U S A (2000) 4.79

Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing. Nature (2000) 4.68

Phenotypes of mouse diabetes and rat fatty due to mutations in the OB (leptin) receptor. Science (1996) 4.59

Genetic diversity and relationships in populations of Bordetella spp. J Bacteriol (1986) 4.48

A population genetic framework for the study of invasive diseases caused by serotype b strains of Haemophilus influenzae. Proc Natl Acad Sci U S A (1985) 4.48

Noncovalent sidewall functionalization of single-walled carbon nanotubes for protein immobilization. J Am Chem Soc (2001) 4.46

Toward a population genetic analysis of Salmonella: genetic diversity and relationships among strains of serotypes S. choleraesuis, S. derby, S. dublin, S. enteritidis, S. heidelberg, S. infantis, S. newport, and S. typhimurium. Proc Natl Acad Sci U S A (1988) 4.43

Genetic diversity and relationships among Streptococcus pyogenes strains expressing serotype M1 protein: recent intercontinental spread of a subclone causing episodes of invasive disease. Infect Immun (1995) 4.40

Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc Natl Acad Sci U S A (1997) 4.29

PCR amplification and restriction endonuclease analysis of a 65-kilodalton heat shock protein gene sequence for taxonomic separation of rapidly growing mycobacteria. J Clin Microbiol (1995) 4.27

Orientation selectivity and the arrangement of horizontal connections in tree shrew striate cortex. J Neurosci (1997) 4.26

Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation. J Biol Chem (2001) 4.23

Specificity of the HP1 chromo domain for the methylated N-terminus of histone H3. EMBO J (2001) 4.23

Novel gp91(phox) homologues in vascular smooth muscle cells : nox1 mediates angiotensin II-induced superoxide formation and redox-sensitive signaling pathways. Circ Res (2001) 4.22

Parkin ubiquitinates the alpha-synuclein-interacting protein, synphilin-1: implications for Lewy-body formation in Parkinson disease. Nat Med (2001) 4.22

Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc Natl Acad Sci U S A (1994) 4.21

Circular intermediates of recombinant adeno-associated virus have defined structural characteristics responsible for long-term episomal persistence in muscle tissue. J Virol (1998) 4.17

Infectivity, transmission, and pathology of human-isolated H7N9 influenza virus in ferrets and pigs. Science (2013) 4.17

Mycobacterium tuberculosis CDC1551 induces a more vigorous host response in vivo and in vitro, but is not more virulent than other clinical isolates. J Immunol (1999) 4.15

Evasion of human innate and acquired immunity by a bacterial homolog of CD11b that inhibits opsonophagocytosis. Nat Med (2001) 4.09

Risk factors for incident radiographic knee osteoarthritis in the elderly: the Framingham Study. Arthritis Rheum (1997) 4.08

The effect of postmenopausal estrogen therapy on bone density in elderly women. N Engl J Med (1993) 4.04

From ab initio quantum mechanics to molecular neurobiology: a cation-pi binding site in the nicotinic receptor. Proc Natl Acad Sci U S A (1998) 3.99

Is Mycobacterium tuberculosis 15,000 years old? J Infect Dis (1994) 3.97

Multiattribute utility function for a comprehensive health status classification system. Health Utilities Index Mark 2. Med Care (1996) 3.96

Molecular epidemiology of methicillin-resistant Staphylococcus aureus in 12 New York hospitals. MRSA Collaborative Study Group. J Infect Dis (1998) 3.94

Evidence for the introduction of a multiresistant clone of serotype 6B Streptococcus pneumoniae from Spain to Iceland in the late 1980s. J Infect Dis (1993) 3.94

The incidence and natural history of knee osteoarthritis in the elderly. The Framingham Osteoarthritis Study. Arthritis Rheum (1995) 3.93

Molecular dynamics simulations of a lipid bilayer and of hexadecane: an investigation of membrane fluidity. Science (1993) 3.81

The Emerging Risk Factors Collaboration: analysis of individual data on lipid, inflammatory and other markers in over 1.1 million participants in 104 prospective studies of cardiovascular diseases. Eur J Epidemiol (2007) 3.81

Inhibition of a Mycobacterium tuberculosis beta-ketoacyl ACP synthase by isoniazid. Science (1998) 3.81

High-level semi-synthetic production of the potent antimalarial artemisinin. Nature (2013) 3.80

A single clone of Staphylococcus aureus causes the majority of cases of toxic shock syndrome. Proc Natl Acad Sci U S A (1990) 3.76

Characterization of pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis. Antimicrob Agents Chemother (1997) 3.75

Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis. J Clin Microbiol (1996) 3.70

Characterization of a virtually full-length human immunodeficiency virus type 1 genome of a prevalent intersubtype (C/B') recombinant strain in China. J Virol (2000) 3.70

Extent and distribution of linkage disequilibrium in three genomic regions. Am J Hum Genet (2000) 3.68

DNA large restriction fragment patterns of sporadic and epidemic nosocomial strains of Mycobacterium chelonae and Mycobacterium abscessus. J Clin Microbiol (1993) 3.65

Role of miR-143 targeting KRAS in colorectal tumorigenesis. Oncogene (2009) 3.64

Refractive Error Study in Children: results from Shunyi District, China. Am J Ophthalmol (2000) 3.62