A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

PubWeight™: 903.81‹?› | Rank: Top 0.01% | All-Time Top 10

🔗 View Article (PMID 942051)

Published in Anal Biochem on May 07, 1976

Authors

M M Bradford

Associated clinical trials:

Short Term Evaluation of the Soothing and Re-epithelizing Activity of Three Topical Products and Relative Combinations | NCT02241161

Efficacy and Tolerance Evaluation of a Topical "Revitalizing" Face Day Cream | NCT02230397

Articles citing this

(truncated to the top 100)

GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J (1987) 57.37

Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A (1992) 35.45

Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol (1987) 26.70

Rapamycin fed late in life extends lifespan in genetically heterogeneous mice. Nature (2009) 19.56

Mechanism of activation of protein kinase B by insulin and IGF-1. EMBO J (1996) 16.68

Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol (1988) 13.78

Complexes between the LKB1 tumor suppressor, STRAD alpha/beta and MO25 alpha/beta are upstream kinases in the AMP-activated protein kinase cascade. J Biol (2003) 12.91

Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1. EMBO J (1991) 11.86

T cell growth factor receptors. Quantitation, specificity, and biological relevance. J Exp Med (1981) 10.66

Hydrolytic enzymes in the central vacuole of plant cells. Plant Physiol (1979) 10.65

Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H(2)O(2)-requiring oxygenase. Proc Natl Acad Sci U S A (1984) 10.26

Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody. Infect Immun (1983) 9.60

Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1981) 9.45

A mitochondrial origin for frontotemporal dementia and amyotrophic lateral sclerosis through CHCHD10 involvement. Brain (2014) 9.30

beta-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci U S A (1986) 9.05

Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc Natl Acad Sci U S A (1987) 8.53

Transforming growth factor-beta 1 induces alpha-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J Cell Biol (1993) 8.36

Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants. Proc Natl Acad Sci U S A (2002) 8.01

Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A beta-lactamase, KPC-3, in a New York Medical Center. Antimicrob Agents Chemother (2004) 7.99

Axin, a negative regulator of the Wnt signaling pathway, forms a complex with GSK-3beta and beta-catenin and promotes GSK-3beta-dependent phosphorylation of beta-catenin. EMBO J (1998) 7.59

A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor. Mol Cell Biol (1986) 7.49

Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide. Proc Natl Acad Sci U S A (1985) 7.36

Changes in the conformation of influenza virus hemagglutinin at the pH optimum of virus-mediated membrane fusion. Proc Natl Acad Sci U S A (1982) 7.34

The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infect Immun (1999) 7.22

Isolation and characterization of homogeneous heat-labile enterotoxins with high specific activity from Escherichia coli cultures. Infect Immun (1979) 7.21

Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl Environ Microbiol (1996) 7.12

Transferable imipenem resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother (1991) 7.10

Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae. Mol Cell Biol (1988) 7.10

Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins. J Virol (1987) 7.05

Glutamate uptake into astrocytes stimulates aerobic glycolysis: a mechanism coupling neuronal activity to glucose utilization. Proc Natl Acad Sci U S A (1994) 6.96

Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay. Proc Natl Acad Sci U S A (1985) 6.94

Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. J Bacteriol (1988) 6.94

Purification and characterization of toxins A and B of Clostridium difficile. Infect Immun (1982) 6.88

Human immunodeficiency virus integration protein expressed in Escherichia coli possesses selective DNA cleaving activity. Proc Natl Acad Sci U S A (1990) 6.85

Variation in a major surface protein of Lyme disease spirochetes. Infect Immun (1984) 6.68

Biological function of pathogenesis-related proteins: Four tobacco pathogenesis-related proteins are chitinases. Proc Natl Acad Sci U S A (1987) 6.67

Bid-induced conformational change of Bax is responsible for mitochondrial cytochrome c release during apoptosis. J Cell Biol (1999) 6.58

Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway. Plant Cell (1996) 6.58

Purification, characterization, and toxicity of the sulfhydryl-activated hemolysin listeriolysin O from Listeria monocytogenes. Infect Immun (1987) 6.47

Morphological effects of lipopeptides against Aspergillus fumigatus correlate with activities against (1,3)-beta-D-glucan synthase. Antimicrob Agents Chemother (1994) 6.45

An Arabidopsis mutant defective in the general phenylpropanoid pathway. Plant Cell (1992) 6.39

Selected reaction monitoring for quantitative proteomics: a tutorial. Mol Syst Biol (2008) 6.38

Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat. Proc Natl Acad Sci U S A (1989) 6.32

The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J (1996) 6.26

Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon. Proc Natl Acad Sci U S A (1982) 6.22

Occurrence of a beta-lactam-inducible penicillin-binding protein in methicillin-resistant staphylococci. Antimicrob Agents Chemother (1985) 6.19

Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell (2000) 6.12

The p65 subunit is responsible for the strong transcription activating potential of NF-kappa B. EMBO J (1991) 6.08

Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence. J Cell Biol (1989) 6.05

Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis. Plant Cell (1998) 6.03

Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A (1996) 5.93

Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis. Mol Cell Biol (1987) 5.93

The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation. EMBO J (1992) 5.92

New class of transforming growth factors potentiated by epidermal growth factor: isolation from non-neoplastic tissues. Proc Natl Acad Sci U S A (1981) 5.89

Bid induces the oligomerization and insertion of Bax into the outer mitochondrial membrane. Mol Cell Biol (2000) 5.85

Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol Cell Biol (1987) 5.84

The intercellular adhesin involved in biofilm accumulation of Staphylococcus epidermidis is a linear beta-1,6-linked glucosaminoglycan: purification and structural analysis. J Bacteriol (1996) 5.82

Using circular dichroism spectra to estimate protein secondary structure. Nat Protoc (2006) 5.81

Regulated internalization of caveolae. J Cell Biol (1994) 5.77

IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth. Plant Cell (2002) 5.77

Biodegradation of trichloroethylene and involvement of an aromatic biodegradative pathway. Appl Environ Microbiol (1987) 5.73

Antigens of Borrelia burgdorferi recognized during Lyme disease. Appearance of a new immunoglobulin M response and expansion of the immunoglobulin G response late in the illness. J Clin Invest (1986) 5.72

Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J (1998) 5.70

Development of "substrate-trapping" mutants to identify physiological substrates of protein tyrosine phosphatases. Proc Natl Acad Sci U S A (1997) 5.63

Gibberellin metabolism in cell-free extracts from spinach leaves in relation to photoperiod. Plant Physiol (1986) 5.62

Variable major proteins of Borrellia hermsii. J Exp Med (1982) 5.62

Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID. Mol Cell Biol (1988) 5.60

Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling. Biochem J (1993) 5.60

Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1. Appl Environ Microbiol (1988) 5.53

Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum. J Bacteriol (1983) 5.49

Effects of Clostridium difficile toxins given intragastrically to animals. Infect Immun (1985) 5.44

c-Myc transactivation of LDH-A: implications for tumor metabolism and growth. Proc Natl Acad Sci U S A (1997) 5.43

Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis. Am J Pathol (1989) 5.41

Protein synthesis in HeLa or Henle 407 cells infected with Shigella dysenteriae 1, Shigella flexneri 2a, or Salmonella typhimurium W118. Infect Immun (1981) 5.39

Coupling of a signal response domain in I kappa B alpha to multiple pathways for NF-kappa B activation. Mol Cell Biol (1995) 5.32

Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes. Nucleic Acids Res (1986) 5.29

Characterization of the metallo-beta-lactamase determinant of Acinetobacter baumannii AC-54/97 reveals the existence of bla(IMP) allelic variants carried by gene cassettes of different phylogeny. Antimicrob Agents Chemother (2000) 5.26

Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines. J Exp Med (1979) 5.22

Enzymatic replication of the origin of the Escherichia coli chromosome. Proc Natl Acad Sci U S A (1981) 5.20

Gene recombination in postmitotic cells. Targeted expression of Cre recombinase provokes cardiac-restricted, site-specific rearrangement in adult ventricular muscle in vivo. J Clin Invest (1997) 5.19

Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties. J Exp Med (1988) 5.16

Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1-expressing microglia. Proc Natl Acad Sci U S A (1998) 5.15

Sustained activation of the mitogen-activated protein (MAP) kinase cascade may be required for differentiation of PC12 cells. Comparison of the effects of nerve growth factor and epidermal growth factor. Biochem J (1992) 5.14

Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho. EMBO J (1996) 5.14

Activation of the pp60c-src kinase by middle T antigen binding or by dephosphorylation. EMBO J (1985) 5.14

Purification and characterization of a newly identified growth factor specific for epithelial cells. Proc Natl Acad Sci U S A (1989) 5.10

Transcriptional activation and chromatin remodeling of the HIV-1 promoter in response to histone acetylation. EMBO J (1996) 5.10

Overexpression of Phytochrome B Induces a Short Hypocotyl Phenotype in Transgenic Arabidopsis. Plant Cell (1991) 5.10

Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol (1984) 5.08

Characterization of L-CAM, a major cell adhesion molecule from embryonic liver cells. Proc Natl Acad Sci U S A (1983) 5.05

Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Mol Cell Biol (1987) 5.03

MAPKAP kinase-2; a novel protein kinase activated by mitogen-activated protein kinase. EMBO J (1992) 4.97

Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors. J Virol (1988) 4.92

LasR of Pseudomonas aeruginosa is a transcriptional activator of the alkaline protease gene (apr) and an enhancer of exotoxin A expression. Infect Immun (1993) 4.90

Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle fusion in vitro. J Cell Biol (1989) 4.88

Flow of information in the light-triggered cyclic nucleotide cascade of vision. Proc Natl Acad Sci U S A (1981) 4.87

Microtubule dynamics in interphase cells. J Cell Biol (1986) 4.85

Specific protein binding to far upstream activating sequences in polymerase II promoters. Proc Natl Acad Sci U S A (1985) 4.85

Biosynthesis of wound ethylene. Plant Physiol (1980) 4.84

The top 100 papers. Nature (2014) 4.83