Published in Proc Natl Acad Sci U S A on August 04, 1998
Macromolecular crowding and confinement: biochemical, biophysical, and potential physiological consequences. Annu Rev Biophys (2008) 7.77
Predicting the energetics of osmolyte-induced protein folding/unfolding. Proc Natl Acad Sci U S A (2005) 2.85
FlgM gains structure in living cells. Proc Natl Acad Sci U S A (2002) 2.46
Protein-solvent preferential interactions, protein hydration, and the modulation of biochemical reactions by solvent components. Proc Natl Acad Sci U S A (2002) 2.17
Protein stability in mixed solvents: a balance of contact interaction and excluded volume. Biophys J (2003) 2.01
Protein folding, stability, and solvation structure in osmolyte solutions. Biophys J (2005) 1.72
Thermodynamics of interactions of urea and guanidinium salts with protein surface: relationship between solute effects on protein processes and changes in water-accessible surface area. Protein Sci (2001) 1.69
Osmotically regulated synthesis of the compatible solute ectoine in Bacillus pasteurii and related Bacillus spp. Appl Environ Microbiol (2002) 1.64
Molecular dynamics of solid-state lysozyme as affected by glycerol and water: a neutron scattering study. Biophys J (2000) 1.59
Destabilization of osteogenesis imperfecta collagen-like model peptides correlates with the identity of the residue replacing glycine. Proc Natl Acad Sci U S A (2000) 1.58
Solvent-induced collapse of alpha-synuclein and acid-denatured cytochrome c. Protein Sci (2001) 1.40
Osmolyte effects on protein stability and solubility: a balancing act between backbone and side-chains. Biophys Chem (2011) 1.35
The osmoreactive betaine carrier BetP from Corynebacterium glutamicum is a sensor for cytoplasmic K+. EMBO J (2001) 1.33
Protein structure, stability and solubility in water and other solvents. Philos Trans R Soc Lond B Biol Sci (2004) 1.25
Solutes modify a conformational transition in a membrane transport protein. Biophys J (2006) 1.20
Probing protein-sugar interactions. Biophys J (2000) 1.16
Chemical chaperone rescue of mutant human cystathionine beta-synthase. Mol Genet Metab (2007) 1.14
Osmoadaptation in archaea Appl Environ Microbiol (1999) 1.13
Backbone additivity in the transfer model of protein solvation. Protein Sci (2010) 1.13
Mimicking the plant cell interior under water stress by macromolecular crowding: disordered dehydrin proteins are highly resistant to structural collapse. Plant Physiol (2008) 1.13
Osmolyte-induced conformational changes in the Hsp90 molecular chaperone. Protein Sci (2010) 1.12
Trimethylamine N-oxide influence on the backbone of proteins: an oligoglycine model. Proteins (2010) 1.12
Hyperosmotic stress response: comparison with other cellular stresses. Pflugers Arch (2007) 1.10
Hydrogen bonding progressively strengthens upon transfer of the protein urea-denatured state to water and protecting osmolytes. Biochemistry (2010) 1.09
Sodium 4-phenylbutyrate ameliorates the effects of cataract-causing mutant gammaD-crystallin in cultured cells. Mol Vis (2010) 1.02
Conformational sampling of peptides in the presence of protein crowders from AA/CG-multiscale simulations. J Phys Chem B (2012) 1.01
Chaperonin-assisted folding of glutamine synthetase under nonpermissive conditions: off-pathway aggregation propensity does not determine the co-chaperonin requirement. Protein Sci (2000) 1.00
Assessing the solvent-dependent surface area of unfolded proteins using an ensemble model. Proc Natl Acad Sci U S A (2008) 1.00
The osmolyte TMAO stabilizes native RNA tertiary structures in the absence of Mg2+: evidence for a large barrier to folding from phosphate dehydration. J Mol Biol (2010) 0.97
Counteracting chemical chaperone effects on the single-molecule α-synuclein structural landscape. Proc Natl Acad Sci U S A (2012) 0.96
The mechanism of VWF-mediated platelet GPIbalpha binding. Biophys J (2010) 0.95
Unfolding and refolding of bovine serum albumin at acid pH: ultrasound and structural studies. Biophys J (2006) 0.94
Molecular mechanism for the preferential exclusion of TMAO from protein surfaces. J Phys Chem B (2012) 0.93
The N(0)-binding region of the vesicular stomatitis virus phosphoprotein is globally disordered but contains transient α-helices. Protein Sci (2011) 0.93
Effect of osmolytes on protein dynamics in the lactate dehydrogenase-catalyzed reaction. Biochemistry (2011) 0.91
Small-angle X-ray scattering of reduced ribonuclease A: effects of solution conditions and comparisons with a computational model of unfolded proteins. J Mol Biol (2008) 0.89
Osmolyte-induced separation of the mechanical folding phases of ubiquitin. Proc Natl Acad Sci U S A (2009) 0.89
The folding free-energy surface of HIV-1 protease: insights into the thermodynamic basis for resistance to inhibitors. J Mol Biol (2009) 0.88
Role of protein stabilizers on the conformation of the unfolded state of cytochrome c and its early folding kinetics: investigation at single molecular resolution. J Biol Chem (2010) 0.88
Homeostasis and catabolism of choline and glycine betaine: lessons from Pseudomonas aeruginosa. Appl Environ Microbiol (2013) 0.87
Protein folding at single-molecule resolution. Biochim Biophys Acta (2011) 0.86
Stabilization of a metastable state of Torpedo californica acetylcholinesterase by chemical chaperones. Protein Sci (2003) 0.86
Osmolyte effects on helix formation in peptides and the stability of coiled-coils. Protein Sci (2002) 0.85
Properties of hydrophobic free energy found by gas-liquid transfer. Proc Natl Acad Sci U S A (2013) 0.85
Osmolyte-induced perturbations of hydrogen bonding between hydration layer waters: correlation with protein conformational changes. J Phys Chem B (2009) 0.84
The sarcosine effect on protein stability: a case of nonadditivity? Protein Sci (2000) 0.84
A bulk water-dependent desolvation energy model for analyzing the effects of secondary solutes on biological equilibria. Biochemistry (2011) 0.83
Urea-temperature phase diagrams capture the thermodynamics of denatured state expansion that accompany protein unfolding. Protein Sci (2013) 0.83
Effects of different osmolytes on the induced folding of the N-terminal activation domain (AF1) of the glucocorticoid receptor. Arch Biochem Biophys (2007) 0.83
The osmolyte trimethylamine-N-oxide stabilizes the Fyn SH3 domain without altering the structure of its folding transition state. Protein Sci (2009) 0.81
Worm-like Ising model for protein mechanical unfolding under the effect of osmolytes. Biophys J (2012) 0.78
Molten globule and native state ensemble of Helicobacter pylori flavodoxin: can crowding, osmolytes or cofactors stabilize the native conformation relative to the molten globule? Biophys J (2008) 0.78
Synergy in protein-osmolyte mixtures. J Phys Chem B (2014) 0.77
Naturally occurring osmolyte, trehalose induces functional conformation in an intrinsically disordered activation domain of glucocorticoid receptor. PLoS One (2011) 0.77
Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose. Enzyme Res (2012) 0.76
A small molecule chemical chaperone optimizes its unfolded state contraction and denaturant like properties. Sci Rep (2013) 0.75
Untangling a Structurally Resolved Protein Folding Intermediate. Biophys J (2016) 0.75
Testing the ability of non-methylamine osmolytes present in kidney cells to counteract the deleterious effects of urea on structure, stability and function of proteins. PLoS One (2013) 0.75
Effect of osmolytes on the binding of EGR1 transcription factor to DNA. Biopolymers (2015) 0.75
TMAO-Protein Preferential Interaction Profile Determines TMAO's Conditional In Vivo Compatibility. Biophys J (2016) 0.75
Thiamine diphosphate binds to intermediates in the assembly of adenovirus fiber knob trimers in Escherichia coli. Protein Sci (2007) 0.75
K+ transport in the caterpillar intestine epithelium: role of osmolytes for the K+-secretory capacity of the tobacco hornworm midgut. J Comp Physiol B (2004) 0.75
The interpretation of protein structures: estimation of static accessibility. J Mol Biol (1971) 40.24
Living with water stress: evolution of osmolyte systems. Science (1982) 17.27
The solubility of amino acids and two glycine peptides in aqueous ethanol and dioxane solutions. Establishment of a hydrophobicity scale. J Biol Chem (1971) 8.06
Protein denaturation. C. Theoretical models for the mechanism of denaturation. Adv Protein Chem (1970) 6.90
The control of protein stability and association by weak interactions with water: how do solvents affect these processes? Annu Rev Biophys Biomol Struct (1993) 4.36
The stabilization of proteins by sucrose. J Biol Chem (1981) 3.34
THE EFFECT OF COMPOUNDS OF THE UREA-GUANIDINIUM CLASS ON THE ACTIVITY COEFFICIENT OF ACETYLTETRAGLYCINE ETHYL ESTER AND RELATED COMPOUNDS. J Am Chem Soc (1965) 3.02
A naturally occurring protective system in urea-rich cells: mechanism of osmolyte protection of proteins against urea denaturation. Biochemistry (1997) 2.79
Stabilization of protein structure by sugars. Biochemistry (1982) 2.67
THE SOLUBILITY OF AMINO ACIDS AND RELATED COMPOUNDS IN AQUEOUS UREA SOLUTIONS. J Biol Chem (1963) 2.64
Regeneration of native secondary and tertiary structures by air oxidation of reduced ribonuclease. J Biol Chem (1961) 2.61
The peptide backbone plays a dominant role in protein stabilization by naturally occurring osmolytes. Biochemistry (1995) 2.39
Forcing thermodynamically unfolded proteins to fold. J Biol Chem (1998) 2.34
Preferential interactions of proteins with salts in concentrated solutions. Biochemistry (1982) 2.29
Why do some organisms use a urea-methylamine mixture as osmolyte? Thermodynamic compensation of urea and trimethylamine N-oxide interactions with protein. Biochemistry (1994) 2.24
Hydrophobicity of amino acid subgroups in proteins. Proteins (1990) 2.10
The solubility of amino acids, diglycine, and triglycine in aqueous guanidine hydrochloride solutions. J Biol Chem (1970) 1.77
Mechanism of protein salting in and salting out by divalent cation salts: balance between hydration and salt binding. Biochemistry (1984) 1.76
Mechanism of poly(ethylene glycol) interaction with proteins. Biochemistry (1985) 1.74
Preferential interactions of proteins with solvent components in aqueous amino acid solutions. Arch Biochem Biophys (1983) 1.68
Modeling unfolded states of proteins and peptides. II. Backbone solvent accessibility. Biochemistry (1997) 1.64
The solubility of amino acids and related compounds in aqueous thylene glycol solutions. J Biol Chem (1965) 1.58
Preferential solvent interactions between proteins and polyethylene glycols. J Biol Chem (1981) 1.52
Preferential interactions determine protein solubility in three-component solutions: the MgCl2 system. Biochemistry (1990) 1.52
Evidence for the instability of hydrogen-bonded peptide structures in water, based on studies of ribonuclease and oxidized ribonuclease. C R Trav Lab Carlsberg Chim (1956) 1.47
Why preferential hydration does not always stabilize the native structure of globular proteins. Biochemistry (1990) 1.43
Protein stabilization and destabilization by guanidinium salts. Biochemistry (1984) 1.32
The mechanism of action of Na glutamate, lysine HCl, and piperazine-N,N'-bis(2-ethanesulfonic acid) in the stabilization of tubulin and microtubule formation. J Biol Chem (1984) 1.27
Thermal stability of proteins in the presence of poly(ethylene glycols). Biochemistry (1987) 1.04
A comparison of the physical chemical properties of oxidized and reduced alkylated ribonuclease. Biochim Biophys Acta (1959) 0.94
Studies on bovine pancreatic ribonuclease A and model compounds in aqueous 2-methyl-2,4-pentanediol. I. Amino acid solubility, thermal reversibility of ribonuclease A, and preferential hydration of ribonuclease A crystals. Arch Biochem Biophys (1971) 0.87
Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants. Biochemistry (1988) 9.18
Calorimetric determination of the enthalpy change for the alpha-helix to coil transition of an alanine peptide in water. Proc Natl Acad Sci U S A (1991) 2.84
A naturally occurring protective system in urea-rich cells: mechanism of osmolyte protection of proteins against urea denaturation. Biochemistry (1997) 2.79
A test of the linear extrapolation of unfolding free energy changes over an extended denaturant concentration range. Biochemistry (1992) 2.73
Increased thermal stability of proteins in the presence of naturally occurring osmolytes. Biochemistry (1992) 2.47
The peptide backbone plays a dominant role in protein stabilization by naturally occurring osmolytes. Biochemistry (1995) 2.39
Forcing thermodynamically unfolded proteins to fold. J Biol Chem (1998) 2.34
Efficacy of macromolecular crowding in forcing proteins to fold. Biophys Chem (2002) 1.52
Trimethylamine N-oxide-induced cooperative folding of an intrinsically unfolded transcription-activating fragment of human glucocorticoid receptor. J Biol Chem (1999) 1.31
How valid are denaturant-induced unfolding free energy measurements? Level of conformance to common assumptions over an extended range of ribonuclease A stability. Biochemistry (1995) 1.27
Interdomain signaling in a two-domain fragment of the human glucocorticoid receptor. J Biol Chem (1999) 1.24
Trimethylamine-N-oxide counteracts urea effects on rabbit muscle lactate dehydrogenase function: a test of the counteraction hypothesis. Biophys J (1998) 1.23
The conformation of the glucocorticoid receptor af1/tau1 domain induced by osmolyte binds co-regulatory proteins. J Biol Chem (2001) 1.23
Thermodynamics of denaturant-induced unfolding of a protein that exhibits variable two-state denaturation. Biochemistry (2004) 1.20
Monitoring the sizes of denatured ensembles of staphylococcal nuclease proteins: implications regarding m values, intermediates, and thermodynamics. Biochemistry (1998) 1.13
Effects of a naturally occurring compatible osmolyte on the internal dynamics of ribonuclease A. Biochemistry (1995) 1.12
The paradox between m values and deltaCp's for denaturation of ribonuclease T1 with disulfide bonds intact and broken. Protein Sci (1999) 1.07
Osmolyte-induced protein folding free energy changes. Proteins (2006) 0.95
Osmolyte effects on kinetics of FKBP12 C22A folding coupled with prolyl isomerization. J Mol Biol (2003) 0.92
Effect of proline on lactate dehydrogenase activity: testing the generality and scope of the compatibility paradigm. Biophys J (1996) 0.88
The peculiar nature of the guanidine hydrochloride-induced two-state denaturation of staphylococcal nuclease: a calorimetric study. Biochemistry (1999) 0.88
The unfolding enthalpy of the pH 4 molten globule of apomyoglobin measured by isothermal titration calorimetry. Protein Sci (2000) 0.81
Calorimetric studies of protein--inhibitor interaction. I. Binding of 3'-cytidine monophosphate to ribonuclease A at pH 5.5. Biochemistry (1971) 0.81
Structural thermodynamics of a random coil protein in guanidine hydrochloride. Proteins (2000) 0.80
Kinetic properties of adenosine deaminase in mixed aqueous solvents. Biochemistry (1969) 0.80
Probes of energy transduction in enzyme catalysis. Methods Enzymol (1995) 0.80
A guanidine hydrochloride induced change in ribonuclease without gross unfolding. Biochem Biophys Res Commun (1978) 0.79
Simultaneous analysis for testing of models and parameter estimation. Methods Enzymol (1992) 0.79
Synthesis, purification, and characterization of the A and B diastereomers of gamma-32P-labeled adenosine 5'-O-(2-thiotriphosphate). J Biol Chem (1980) 0.77
Time-dependent effects of trimethylamine-N-oxide/urea on lactate dehydrogenase activity: an unexplored dimension of the adaptation paradigm. Biophys J (1998) 0.77
Stereochemical and kinetic studies on the action of the catalytic subunit of bovine cardiac muscle adenosine 3',5'-monophosphate dependent protein kinase using metal ion complexes of ATP beta S. Biochemistry (1980) 0.77
Gambling and the gambler. A review and preliminary findings. Arch Gen Psychiatry (1968) 0.77
Covalent bond changes as a driving force in enzyme catalysis. Biochemistry (1993) 0.77
Energetics of alpha-chymotrypsin-mediated hydrolysis of a strained cyclic ester. Biochemistry (1987) 0.75
Treatment of compulsive gambling. Lancet (1968) 0.75
The compulsive gambler and spouse in group psychotherapy. Int J Group Psychother (1970) 0.75
Thermal behavior of HeLa and KB cells in suspension and attached to glass. Biochim Biophys Acta (1977) 0.75
The Gibbs conference on biothermodynamics: origins and evolution. Biophys Chem (1997) 0.75
A diaphragm delivery device for flow microcalorimetry. Anal Biochem (1978) 0.75
Thermodynamics of zinc binding to rhodanese. Arch Biochem Biophys (1974) 0.75