Life and death of a signal peptide.

PubWeight™: 1.35‹?› | Rank: Top 10%

🔗 View Article (PMID 9823886)

Published in Nature on November 12, 1998

Authors

G von Heijne

Articles citing this

Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome. Microbiol Mol Biol Rev (2000) 3.81

Proteomics of protein secretion by Bacillus subtilis: separating the "secrets" of the secretome. Microbiol Mol Biol Rev (2004) 2.67

Subcellular localization and topology of the p7 polypeptide of hepatitis C virus. J Virol (2002) 1.89

Asparagine-proline sequence within membrane-spanning segment of SREBP triggers intramembrane cleavage by site-2 protease. Proc Natl Acad Sci U S A (2000) 1.62

Mass spectrometry-based proteomics: existing capabilities and future directions. Chem Soc Rev (2012) 1.47

Mutagenesis of the signal sequence of yellow fever virus prM protein: enhancement of signalase cleavage In vitro is lethal for virus production. J Virol (2000) 1.35

Signal peptide cleavage of a type I membrane protein, HCMV US11, is dependent on its membrane anchor. EMBO J (2001) 1.24

RANK, RANKL and osteoprotegerin in bone biology and disease. Curr Rev Musculoskelet Med (2009) 1.17

Mapping the landscape of the lymphocytic choriomeningitis virus stable signal peptide reveals novel functional domains. J Virol (2007) 1.09

Construction and mutagenesis of an artificial bicistronic tick-borne encephalitis virus genome reveals an essential function of the second transmembrane region of protein e in flavivirus assembly. J Virol (2006) 1.01

Differential roles of individual domains in selection of secretion route of a Streptococcus parasanguinis serine-rich adhesin, Fap1. J Bacteriol (2007) 0.93

FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion. J Bacteriol (2004) 0.92

Secretory signal peptide modification for optimized antibody-fragment expression-secretion in Leishmania tarentolae. Microb Cell Fact (2012) 0.89

P80, the HinT interacting membrane protein, is a secreted antigen of Mycoplasma hominis. BMC Microbiol (2004) 0.88

How are the non-classically secreted bacterial proteins released into the extracellular milieu? Curr Microbiol (2013) 0.86

A new type of signal peptidase cleavage site identified in an RNA virus polyprotein. J Biol Chem (2010) 0.84

Novel GFP expression using a short N-terminal polypeptide through the defined twin-arginine translocation (Tat) pathway. Mol Cells (2011) 0.84

Use of endogenous signal sequences for transient production and efficient secretion by moss (Physcomitrella patens) cells. BMC Biotechnol (2005) 0.82

The signal peptide of Staphylococcus aureus panton valentine leukocidin LukS component mediates increased adhesion to heparan sulfates. PLoS One (2009) 0.81

Structural analysis of hepatitis C virus core-E1 signal peptide and requirements for cleavage of the genotype 3a signal sequence by signal peptide peptidase. J Virol (2012) 0.81

Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration. J Zhejiang Univ Sci B (2012) 0.80

Thiol redox requirements and substrate specificities of recombinant cytochrome c assembly systems II and III. Biochim Biophys Acta (2011) 0.80

Efficient production of CYTL1 protein using mouse IgGκ signal peptide in the CHO cell expression system. Acta Biochim Biophys Sin (Shanghai) (2016) 0.78

Ion Channel Function and Cross-Species Determinants in Viral Assembly of Nonprimate Hepacivirus p7. J Virol (2016) 0.76

Competitive Inhibition of the Endoplasmic Reticulum Signal Peptidase by Non-cleavable Mutant Preprotein Cargos. J Biol Chem (2015) 0.75

Fluorescence spectroscopy of soluble E. coli SPase I Δ2-75 reveals conformational changes in response to ligand binding. Proteins (2013) 0.75

Identification and molecular characterisation of a homozygous missense mutation in the ADAMTS10 gene in a patient with Weill-Marchesani syndrome. Eur J Hum Genet (2014) 0.75

Articles by these authors

(truncated to the top 100)

Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol (2001) 66.87

Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng (1997) 38.38

A new method for predicting signal sequence cleavage sites. Nucleic Acids Res (1986) 37.19

Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem (1983) 26.68

Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol (2000) 22.77

Signal sequences. The limits of variation. J Mol Biol (1985) 21.24

A hidden Markov model for predicting transmembrane helices in protein sequences. Proc Int Conf Intell Syst Mol Biol (1998) 14.18

How signal sequences maintain cleavage specificity. J Mol Biol (1984) 11.03

TopPred II: an improved software for membrane protein structure predictions. Comput Appl Biosci (1994) 10.03

ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci (1999) 9.82

Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method. Protein Eng (1997) 8.25

Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Sci (1998) 7.88

A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Int J Neural Syst (1999) 7.40

Mitochondrial targeting sequences may form amphiphilic helices. EMBO J (1986) 7.11

Machine learning approaches for the prediction of signal peptides and other protein sorting signals. Protein Eng (1999) 6.72

Sequence determinants of cytosolic N-terminal protein processing. Eur J Biochem (1986) 5.59

A conserved cleavage-site motif in chloroplast transit peptides. FEBS Lett (1990) 4.25

Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering. Protein Eng (1990) 4.17

How proteins adapt to a membrane-water interface. Trends Biochem Sci (2000) 3.76

Trans-membrane translocation of proteins. The direct transfer model. Eur J Biochem (1979) 3.13

Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane. J Biol Chem (1993) 3.02

Predicting the topology of eukaryotic membrane proteins. Eur J Biochem (1993) 2.73

YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase. EMBO J (2000) 2.70

Membrane proteins: the amino acid composition of membrane-penetrating segments. Eur J Biochem (1981) 2.52

Analysis of the distribution of charged residues in the N-terminal region of signal sequences: implications for protein export in prokaryotic and eukaryotic cells. EMBO J (1984) 2.43

Cleavage-site motifs in mitochondrial targeting peptides. Protein Eng (1990) 2.38

The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J (1998) 2.35

A receptor component of the chloroplast protein translocation machinery. Science (1994) 2.34

Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett (2001) 2.28

Fine-tuning the topology of a polytopic membrane protein: role of positively and negatively charged amino acids. Cell (1990) 2.24

On the hydrophobic nature of signal sequences. Eur J Biochem (1981) 2.01

Competition between Sec- and TAT-dependent protein translocation in Escherichia coli. EMBO J (1999) 1.95

Prediction of organellar targeting signals. Biochim Biophys Acta (2001) 1.93

Structures of N-terminally acetylated proteins. Eur J Biochem (1985) 1.92

Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle. FEBS Lett (1996) 1.89

Topology, subcellular localization, and sequence diversity of the Mlo family in plants. J Biol Chem (1999) 1.88

Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor. Mol Microbiol (1997) 1.85

Net N-C charge imbalance may be important for signal sequence function in bacteria. J Mol Biol (1986) 1.85

Topological rules for membrane protein assembly in eukaryotic cells. J Biol Chem (1997) 1.83

Amino acid distributions around O-linked glycosylation sites. Biochem J (1991) 1.82

Anionic phospholipids are determinants of membrane protein topology. EMBO J (1997) 1.77

Molecular mechanism of membrane protein integration into the endoplasmic reticulum. Cell (1997) 1.70

Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli. Proc Natl Acad Sci U S A (1998) 1.62

Topological "frustration" in multispanning E. coli inner membrane proteins. Cell (1994) 1.59

Consensus predictions of membrane protein topology. FEBS Lett (2000) 1.55

Membrane protein topology: effects of delta mu H+ on the translocation of charged residues explain the 'positive inside' rule. EMBO J (1994) 1.53

Translation rate modification by preferential codon usage: intragenic position effects. J Theor Biol (1987) 1.51

A nascent secretory protein may traverse the ribosome/endoplasmic reticulum translocase complex as an extended chain. J Biol Chem (1996) 1.51

The aromatic residues Trp and Phe have different effects on the positioning of a transmembrane helix in the microsomal membrane. Biochemistry (1999) 1.49

Towards a comparative anatomy of N-terminal topogenic protein sequences. J Mol Biol (1986) 1.46

Chloroplast transit peptides from the green alga Chlamydomonas reinhardtii share features with both mitochondrial and higher plant chloroplast presequences. FEBS Lett (1990) 1.45

A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli. Proc Natl Acad Sci U S A (1991) 1.43

The distribution of charged amino acids in mitochondrial inner-membrane proteins suggests different modes of membrane integration for nuclearly and mitochondrially encoded proteins. Eur J Biochem (1992) 1.42

Sec dependent and sec independent assembly of E. coli inner membrane proteins: the topological rules depend on chain length. EMBO J (1993) 1.41

Feature-extraction from endopeptidase cleavage sites in mitochondrial targeting peptides. Proteins (1998) 1.41

The 'positive-inside rule' applies to thylakoid membrane proteins. FEBS Lett (1991) 1.40

The COOH-terminal ends of internal signal and signal-anchor sequences are positioned differently in the ER translocase. J Cell Biol (1994) 1.39

Architecture of helix bundle membrane proteins: an analysis of cytochrome c oxidase from bovine mitochondria. Protein Sci (1997) 1.38

A turn propensity scale for transmembrane helices. J Mol Biol (1999) 1.37

Determination of the border between the transmembrane and cytoplasmic domains of human integrin subunits. J Biol Chem (1999) 1.37

Signal sequences are not uniformly hydrophobic. J Mol Biol (1982) 1.37

Turns in transmembrane helices: determination of the minimal length of a "helical hairpin" and derivation of a fine-grained turn propensity scale. J Mol Biol (1999) 1.36

Stop-transfer function of pseudo-random amino acid segments during translocation across prokaryotic and eukaryotic membranes. Eur J Biochem (1998) 1.34

Different conformations of nascent polypeptides during translocation across the ER membrane. BMC Cell Biol (2000) 1.34

Defining a similarity threshold for a functional protein sequence pattern: the signal peptide cleavage site. Proteins (1996) 1.31

Positively and negatively charged residues have different effects on the position in the membrane of a model transmembrane helix. J Mol Biol (1998) 1.27

Proline-induced disruption of a transmembrane alpha-helix in its natural environment. J Mol Biol (1998) 1.26

A 12-residue-long polyleucine tail is sufficient to anchor synaptobrevin to the endoplasmic reticulum membrane. J Biol Chem (1996) 1.25

Properties of N-terminal tails in G-protein coupled receptors: a statistical study. Protein Eng (1995) 1.25

Glycosylation efficiency of Asn-Xaa-Thr sequons depends both on the distance from the C terminus and on the presence of a downstream transmembrane segment. J Biol Chem (2000) 1.25

Forced transmembrane orientation of hydrophilic polypeptide segments in multispanning membrane proteins. Mol Cell (1998) 1.25

Why mitochondria need a genome. FEBS Lett (1986) 1.23

Divergent evolution of membrane protein topology: the Escherichia coli RnfA and RnfE homologues. Proc Natl Acad Sci U S A (1999) 1.22

Sec-independent translocation of a 100-residue periplasmic N-terminal tail in the E. coli inner membrane protein proW. EMBO J (1994) 1.21

Prediction of N-terminal protein sorting signals. Curr Opin Struct Biol (1997) 1.18

Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: a rapid way to map helix-helix interactions in integral membrane proteins. Protein Sci (1996) 1.15

Membrane topology of the 60-kDa Oxa1p homologue from Escherichia coli. J Biol Chem (1998) 1.14

Positively charged amino acids placed next to a signal sequence block protein translocation more efficiently in Escherichia coli than in mammalian microsomes. Mol Gen Genet (1993) 1.13

Different positively charged amino acids have similar effects on the topology of a polytopic transmembrane protein in Escherichia coli. J Biol Chem (1992) 1.10

Mapping of catalytically important domains in Escherichia coli leader peptidase. EMBO J (1990) 1.08

Homology to region X from staphylococcal protein A is not unique to cell surface proteins. J Theor Biol (1987) 1.07

Phosphatidylethanolamine mediates insertion of the catalytic domain of leader peptidase in membranes. FEBS Lett (1998) 1.04

Three-dimensional model for the membrane domain of Escherichia coli leader peptidase based on disulfide mapping. Biochemistry (1993) 1.02

Analysis and prediction of mitochondrial targeting peptides. Methods Cell Biol (2001) 1.02

Distant downstream sequence determinants can control N-tail translocation during protein insertion into the endoplasmic reticulum membrane. J Biol Chem (2000) 1.01

Do protein-lipid interactions determine the recognition of transmembrane helices at the ER translocon? Biochem Soc Trans (2005) 1.01

Architecture of beta-barrel membrane proteins: analysis of trimeric porins. Protein Sci (1998) 1.00

A signal peptide with a proline next to the cleavage site inhibits leader peptidase when present in a sec-independent protein. FEBS Lett (1992) 1.00

Trans-membrane translocation of proteins. A detailed physico-chemical analysis. Eur J Biochem (1980) 1.00

De novo design of integral membrane proteins. Nat Struct Biol (1994) 0.99

Directionality in protein translocation across membranes: the N-tail phenomenon. Trends Cell Biol (1995) 0.98

Inhibition of protein translocation across the endoplasmic reticulum membrane by sterols. J Biol Chem (2001) 0.95

Membrane topology of Kch, a putative K+ channel from Escherichia coli. J Biol Chem (1996) 0.94

The internal repeats in the Na+/Ca2+ exchanger-related Escherichia coli protein YrbG have opposite membrane topologies. J Biol Chem (2001) 0.93

Breaking the camel's back: proline-induced turns in a model transmembrane helix. J Mol Biol (1998) 0.93

Alanine insertion scanning mutagenesis of lactose permease transmembrane helices. J Biol Chem (1997) 0.92

In vitro membrane integration of leader peptidase depends on the Sec machinery and anionic phospholipids and can occur post-translationally. FEBS Lett (1997) 0.91

N-tail translocation in a eukaryotic polytopic membrane protein: synergy between neighboring transmembrane segments. Eur J Biochem (1999) 0.91

Helical sidedness and the distribution of polar residues in trans-membrane helices. J Mol Biol (1983) 0.91

A de novo designed signal peptide cleavage cassette functions in vivo. J Biol Chem (1991) 0.91