Engineering chloroplasts: an alternative site for foreign genes, proteins, reactions and products.

PubWeight™: 2.08‹?› | Rank: Top 2%

🔗 View Article (PMID 10802561)

Published in Trends Biotechnol on June 01, 2000

Authors

L Bogorad1

Author Affiliations

1: Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA. bogorad@biosun.harvard.edu

Articles citing this

Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat Biotechnol (2001) 2.46

Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J Mol Biol (2001) 2.44

Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi. Plant Physiol (2001) 1.93

Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confers enhanced salt tolerance. Plant Physiol (2004) 1.79

Gene transfer from organelles to the nucleus: frequent and in big chunks. Proc Natl Acad Sci U S A (2003) 1.68

A chloroplast transgenic approach to hyper-express and purify Human Serum Albumin, a protein highly susceptible to proteolytic degradation. Plant Biotechnol J (2003) 1.64

Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology. Trends Plant Sci (2002) 1.56

Breakthrough in chloroplast genetic engineering of agronomically important crops. Trends Biotechnol (2005) 1.52

Retracted Stable plastid transformation for high-level recombinant protein expression: promises and challenges. J Biomed Biotechnol (2012) 1.38

Characterization of heterologous multigene operons in transgenic chloroplasts: transcription, processing, and translation. Plant Physiol (2005) 1.33

Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Mol Biol (2004) 1.30

Multigene engineering: dawn of an exciting new era in biotechnology. Curr Opin Biotechnol (2002) 1.29

Complete plastid genome sequence of Daucus carota: implications for biotechnology and phylogeny of angiosperms. BMC Genomics (2006) 1.17

The stem-loop region of the tobacco psbA 5'UTR is an important determinant of mRNA stability and translation efficiency. Mol Genet Genomics (2003) 1.08

Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons. Plant J (2007) 1.07

Plastid transformation in the monocotyledonous cereal crop, rice (Oryza sativa) and transmission of transgenes to their progeny. Mol Cells (2006) 1.00

Chloroplast-derived vaccine antigens and biopharmaceuticals: expression, folding, assembly and functionality. Curr Top Microbiol Immunol (2009) 0.94

The dynamic history of plastid genomes in the Campanulaceae sensu lato is unique among angiosperms. Proc Natl Acad Sci U S A (2014) 0.91

Targeting a nuclear anthranilate synthase alpha-subunit gene to the tobacco plastid genome results in enhanced tryptophan biosynthesis. Return of a gene to its pre-endosymbiotic origin. Plant Physiol (2001) 0.90

Targeting tryptophan decarboxylase to selected subcellular compartments of tobacco plants affects enzyme stability and in vivo function and leads to a lesion-mimic phenotype. Plant Physiol (2002) 0.89

Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast. BMC Biotechnol (2009) 0.86

Construction of novel chloroplast expression vector and development of an efficient transformation system for the diatom Phaeodactylum tricornutum. Mar Biotechnol (NY) (2014) 0.82

Tobacco ribosomal DNA spacer element elevates Bowman-Birk inhibitor expression in tomato plants. Plant Cell Rep (2006) 0.77

PCR analysis of pulsed-field gel electrophoresis-purified plastid DNA, a sensitive tool to judge the hetero-/homoplastomic status of plastid transformants. Curr Genet (2003) 0.76

Chloroplast genetic engineering. Trends Biotechnol (2001) 0.75

Fluorescent screening of transgenic Arabidopsis seeds without germination. Plant Physiol (2004) 0.75

Genetic and metabolic engineering in diatoms. Philos Trans R Soc Lond B Biol Sci (2017) 0.75