Published in Methods Enzymol on January 01, 2000
Interactome networks and human disease. Cell (2011) 7.16
Engineering BioBrick vectors from BioBrick parts. J Biol Eng (2008) 5.98
Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res (2004) 5.81
Identification of potential interaction networks using sequence-based searches for conserved protein-protein interactions or "interologs". Genome Res (2001) 5.10
A public genome-scale lentiviral expression library of human ORFs. Nat Methods (2011) 4.98
hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics (2007) 4.62
A Drosophila full-length cDNA resource. Genome Biol (2002) 3.67
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids. Biotechniques (2010) 3.62
Universal protein-binding microarrays for the comprehensive characterization of the DNA-binding specificities of transcription factors. Nat Protoc (2009) 3.48
Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res (2004) 3.36
A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae. Yeast (2007) 3.30
A first version of the Caenorhabditis elegans Promoterome. Genome Res (2004) 3.29
A compendium of Caenorhabditis elegans regulatory transcription factors: a resource for mapping transcription regulatory networks. Genome Biol (2005) 3.24
A gateway-compatible yeast one-hybrid system. Genome Res (2004) 3.23
A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Nucleic Acids Res (2007) 3.22
A C. elegans genome-scale microRNA network contains composite feedback motifs with high flux capacity. Genes Dev (2008) 3.19
Hepatitis C virus infection protein network. Mol Syst Biol (2008) 2.91
Proteome-scale purification of human proteins from bacteria. Proc Natl Acad Sci U S A (2002) 2.88
Systematic sequencing of cDNA clones using the transposon Tn5. Nucleic Acids Res (2002) 2.78
A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome. EMBO Rep (2001) 2.74
Gateway compatible vectors for analysis of gene function in the zebrafish. Dev Dyn (2007) 2.74
Circular polymerase extension cloning of complex gene libraries and pathways. PLoS One (2009) 2.74
Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA promoter activity. Genome Res (2008) 2.52
Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli. Protein Sci (2002) 2.51
Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: functional analysis of multi-segment expression clones. Genome Res (2004) 2.49
High-throughput fluorescent tagging of full-length Arabidopsis gene products in planta. Plant Physiol (2004) 2.48
Thirty-one flavors of Drosophila rab proteins. Genetics (2007) 2.46
Quantitative genome-scale analysis of protein localization in an asymmetric bacterium. Proc Natl Acad Sci U S A (2009) 2.33
The transcriptome of Toxoplasma gondii. BMC Biol (2005) 2.06
Next-generation sequencing to generate interactome datasets. Nat Methods (2011) 2.06
Isoform discovery by targeted cloning, 'deep-well' pooling and parallel sequencing. Nat Methods (2008) 2.03
C. elegans ORFeome version 3.1: increasing the coverage of ORFeome resources with improved gene predictions. Genome Res (2004) 1.92
Structural genomics: a pipeline for providing structures for the biologist. Protein Sci (2002) 1.91
Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries. Nat Protoc (2011) 1.86
Generation of the Brucella melitensis ORFeome version 1.1. Genome Res (2004) 1.86
Biochemical characterization of Arabidopsis complexes containing CONSTITUTIVELY PHOTOMORPHOGENIC1 and SUPPRESSOR OF PHYA proteins in light control of plant development. Plant Cell (2008) 1.85
Receptor-binding protein of Lactococcus lactis phages: identification and characterization of the saccharide receptor-binding site. J Bacteriol (2006) 1.85
Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression. BMC Biotechnol (2004) 1.83
Transcription factor modularity in a gene-centered C. elegans core neuronal protein-DNA interaction network. Genome Res (2007) 1.79
'Edgetic' perturbation of a C. elegans BCL2 ortholog. Nat Methods (2009) 1.74
RAD-51-dependent and -independent roles of a Caenorhabditis elegans BRCA2-related protein during DNA double-strand break repair. Mol Cell Biol (2005) 1.74
FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method. BMC Biotechnol (2011) 1.72
The cytochrome P450 enzyme CYP96A15 is the midchain alkane hydroxylase responsible for formation of secondary alcohols and ketones in stem cuticular wax of Arabidopsis. Plant Physiol (2007) 1.71
Genome-wide coactivation analysis of PGC-1alpha identifies BAF60a as a regulator of hepatic lipid metabolism. Cell Metab (2008) 1.65
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors. Microb Cell Fact (2005) 1.59
Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing. Cell (2016) 1.58
Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium. Proc Natl Acad Sci U S A (2005) 1.49
Caenorhabditis elegans OSR-1 regulates behavioral and physiological responses to hyperosmotic environments. Genetics (2004) 1.49
Closing in on the C. elegans ORFeome by cloning TWINSCAN predictions. Genome Res (2005) 1.48
A large-scale overexpression screen in Saccharomyces cerevisiae identifies previously uncharacterized cell cycle genes. Proc Natl Acad Sci U S A (2001) 1.45
Regulation of extracellular matrix organization by BMP signaling in Caenorhabditis elegans. PLoS One (2014) 1.44
The FLYWCH transcription factors FLH-1, FLH-2, and FLH-3 repress embryonic expression of microRNA genes in C. elegans. Genes Dev (2008) 1.42
A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency. Mol Cell Proteomics (2010) 1.41
Enhanced yeast one-hybrid assays for high-throughput gene-centered regulatory network mapping. Nat Methods (2011) 1.41
Functional modularity of nuclear hormone receptors in a Caenorhabditis elegans metabolic gene regulatory network. Mol Syst Biol (2010) 1.40
A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos. PLoS One (2007) 1.36
One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies. Appl Environ Microbiol (2012) 1.33
A zebrafish model of tauopathy allows in vivo imaging of neuronal cell death and drug evaluation. J Clin Invest (2009) 1.32
Systematic targeted mutagenesis of Brucella melitensis 16M reveals a major role for GntR regulators in the control of virulence. Infect Immun (2005) 1.32
High-throughput expression of C. elegans proteins. Genome Res (2004) 1.32
Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS. EMBO J (2004) 1.24
Delivery of multiple transgenes to plant cells. Plant Physiol (2007) 1.22
Crystal structure of the receptor-binding protein head domain from Lactococcus lactis phage bIL170. J Virol (2006) 1.22
Metabolic network analysis integrated with transcript verification for sequenced genomes. Nat Methods (2009) 1.21
ORFDB: an information resource linking scientific content to a high-quality Open Reading Frame (ORF) collection. Nucleic Acids Res (2004) 1.19
Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis. Appl Environ Microbiol (2011) 1.18
Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance. Nucleic Acids Res (2009) 1.17
Evidence for transcript networks composed of chimeric RNAs in human cells. PLoS One (2012) 1.16
The SARS-coronavirus-host interactome: identification of cyclophilins as target for pan-coronavirus inhibitors. PLoS Pathog (2011) 1.14
Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. Nat Methods (2011) 1.12
Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus. Appl Environ Microbiol (2006) 1.09
High-throughput generation of P. falciparum functional molecules by recombinational cloning. Genome Res (2004) 1.07
New gateways to discovery. Plant Physiol (2007) 1.05
The ORFeome of Staphylococcus aureus v 1.1. BMC Genomics (2008) 1.03
Analysis of the yeast kinome reveals a network of regulated protein localization during filamentous growth. Mol Biol Cell (2008) 1.03
Development of an optimized interaction-mating protocol for large-scale yeast two-hybrid analyses. Genome Biol (2001) 1.01
The C. elegans Snail homolog CES-1 can activate gene expression in vivo and share targets with bHLH transcription factors. Nucleic Acids Res (2009) 1.01
Analysis of microRNA expression and function. Methods Cell Biol (2011) 1.00
Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints. PLoS One (2012) 1.00
Exploration of human ORFeome: high-throughput preparation of ORF clones and efficient characterization of their protein products. DNA Res (2008) 0.98
The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis. Mol Cell Proteomics (2006) 0.97
Heterologous expression of membrane proteins: choosing the appropriate host. PLoS One (2011) 0.97
High-throughput cloning and expression library creation for functional proteomics. Proteomics (2013) 0.96
Novel antigen identification method for discovery of protective malaria antigens by rapid testing of DNA vaccines encoding exons from the parasite genome. Infect Immun (2004) 0.96
A versatile overexpression strategy in the pathogenic yeast Candida albicans: identification of regulators of morphogenesis and fitness. PLoS One (2012) 0.95
A high-throughput platform for lentiviral overexpression screening of the human ORFeome. PLoS One (2011) 0.94
The human PDZome: a gateway to PSD95-Disc large-zonula occludens (PDZ)-mediated functions. Mol Cell Proteomics (2013) 0.94
Large-scale RACE approach for proactive experimental definition of C. elegans ORFeome. Genome Res (2009) 0.93
Molecular mapping of the RNA Cap 2'-O-methyltransferase activation interface between severe acute respiratory syndrome coronavirus nsp10 and nsp16. J Biol Chem (2010) 0.93
Serological microarray for a paradoxical diagnostic of Whipple's disease. Eur J Clin Microbiol Infect Dis (2008) 0.93
Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. PLoS One (2015) 0.93
Recombinatorial cloning using heterologous lox sites. Genome Res (2004) 0.92
Crystal structure and self-interaction of the type VI secretion tail-tube protein from enteroaggregative Escherichia coli. PLoS One (2014) 0.92
Determining the sub-cellular localization of proteins within Caenorhabditis elegans body wall muscle. PLoS One (2011) 0.92
Transcription Factor Activity Mapping of a Tissue-Specific in vivo Gene Regulatory Network. Cell Syst (2015) 0.92
Unique function of Kinesin Kif5A in localization of mitochondria in axons. J Neurosci (2014) 0.91
A pair of ligation-independent Escherichia coli expression vectors for rapid addition of a polyhistidine affinity tag to the N- or C-termini of recombinant proteins. J Biomol Tech (2009) 0.91
lin-8, which antagonizes Caenorhabditis elegans Ras-mediated vulval induction, encodes a novel nuclear protein that interacts with the LIN-35 Rb protein. Genetics (2005) 0.90
Genetic interactions of a putative Arabidopsis thaliana ubiquitin-ligase with components of the Saccharomyces cerevisiae ubiquitination machinery. Curr Genet (2006) 0.89
The Flp double cross system a simple efficient procedure for cloning DNA fragments. BMC Biotechnol (2003) 0.89
Protein interaction mapping in C. elegans using proteins involved in vulval development. Science (2000) 11.61
Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene (1990) 10.41
DNA cloning using in vitro site-specific recombination. Genome Res (2000) 10.14
Nucleotide sequence of the yeast plasmid. Nature (1980) 7.64
Correlation between transcriptome and interactome mapping data from Saccharomyces cerevisiae. Nat Genet (2001) 7.16
A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F. Cell (1992) 6.32
Inhibition of cell proliferation by p107, a relative of the retinoblastoma protein. Genes Dev (1993) 5.54
Identification of potential interaction networks using sequence-based searches for conserved protein-protein interactions or "interologs". Genome Res (2001) 5.10
A dominant control region from the human beta-globin locus conferring integration site-independent gene expression. Nature (1989) 4.17
Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity. Genes Dev (1998) 4.06
Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans. Nat Genet (2001) 4.03
In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA. Nat New Biol (1972) 4.01
The retinoblastoma protein binds to a family of E2F transcription factors. Mol Cell Biol (1993) 3.75
Identification of a family of human F-box proteins. Curr Biol (1999) 3.31
High-throughput yeast two-hybrid assays for large-scale protein interaction mapping. Methods (2001) 3.09
E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle. Proc Natl Acad Sci U S A (1995) 2.99
Cloning and characterization of the DNA of a new human papillomavirus from a woman with dysplasia of the uterine cervix. J Virol (1986) 2.93
RBF, a novel RB-related gene that regulates E2F activity and interacts with cyclin E in Drosophila. Genes Dev (1996) 2.92
Hepatitis C virus infection protein network. Mol Syst Biol (2008) 2.91
Cables links Cdk5 and c-Abl and facilitates Cdk5 tyrosine phosphorylation, kinase upregulation, and neurite outgrowth. Neuron (2000) 2.82
The nucleotide sequences of the untranslated 5' regions of human alpha- and beta-globin mRNAs. Proc Natl Acad Sci U S A (1977) 2.75
A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome. EMBO Rep (2001) 2.74
Construction of a functional human suppressor tRNA gene: an approach to gene therapy for beta-thalassaemia. Nature (1982) 2.65
Tissue specific and position independent expression of the complete gene domain for chicken lysozyme in transgenic mice. EMBO J (1990) 2.56
A novel member of the RING finger family, KRIP-1, associates with the KRAB-A transcriptional repressor domain of zinc finger proteins. Proc Natl Acad Sci U S A (1996) 2.54
Sequences 5' of the bovine keratin 5 gene direct tissue- and cell-type-specific expression of a lacZ gene in the adult and during development. Differentiation (1994) 2.53
The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol Cell Biol (1989) 2.52
Yeast two-hybrid systems and protein interaction mapping projects for yeast and worm. Yeast (2000) 2.51
Oncogenic association of specific human papillomavirus types with cervical neoplasia. J Natl Cancer Inst (1987) 2.45
Screening patients for heterozygous p53 mutations using a functional assay in yeast. Nat Genet (1993) 2.40
Protein interaction maps for model organisms. Nat Rev Mol Cell Biol (2001) 2.32
Mammalian homologs of seven in absentia regulate DCC via the ubiquitin-proteasome pathway. Genes Dev (1997) 2.26
Mice doubly deficient for the Polycomb Group genes Mel18 and Bmi1 reveal synergy and requirement for maintenance but not initiation of Hox gene expression. Development (2001) 2.21
Epidemiology of work related neck and upper limb problems: psychosocial and personal risk factors (part I) and effective interventions from a bio behavioural perspective (part II). J Occup Rehabil (2006) 2.18
RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J (1999) 2.11
[Evaluation of functional capability after hospital admission of nonagenarian patients]. Med Clin (Barc) (2000) 2.01
Nucleotide sequence of human papillomavirus type 31: a cervical neoplasia-associated virus. Virology (1989) 1.95
Cloning multiple copies of a DNA segment. Gene (1981) 1.79
Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia. Proc Natl Acad Sci U S A (1977) 1.75
Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain. EMBO J (1997) 1.67
High-level, erythroid-specific expression of the human alpha-globin gene in transgenic mice and the production of human hemoglobin in murine erythrocytes. Genes Dev (1989) 1.63
Exosome secretion, including the DNA damage-induced p53-dependent secretory pathway, is severely compromised in TSAP6/Steap3-null mice. Cell Death Differ (2008) 1.58
Cloning and expression of the Pst I restriction-modification system in Escherichia coli. Proc Natl Acad Sci U S A (1981) 1.57
Serum angiotensin-converting enzyme. Isolation and relationship to the pulmonary enzyme. J Biol Chem (1977) 1.56
Genetic organization of plasmid R1162 DNA involved in conjugative mobilization. J Bacteriol (1986) 1.55
A new type of papillomavirus associated with cancer of the uterine cervix. Virology (1987) 1.53
Analysis of individual human papillomavirus types in cervical neoplasia: a possible role for type 18 in rapid progression. Am J Obstet Gynecol (1988) 1.52
Loss- and gain-of-function mutations show a polycomb group function for Ring1A in mice. Development (2000) 1.50
Utilizing uracil DNA glycosylase to control carryover contamination in PCR: characterization of residual UDG activity following thermal cycling. Biotechniques (1992) 1.48
The Caenorhabditis elegans gene ncc-1 encodes a cdc2-related kinase required for M phase in meiotic and mitotic cell divisions, but not for S phase. Development (1999) 1.46
lin-35 Rb and cki-1 Cip/Kip cooperate in developmental regulation of G1 progression in C. elegans. Development (2001) 1.45
Production of monoclonal antibodies against mammalian Ring1B proteins. Hybridoma (2001) 1.44
S and G2 phase roles for Cdk2 revealed by inducible expression of a dominant-negative mutant in human cells. Mol Cell Biol (2001) 1.44
Dominant-negative p53 mutations selected in yeast hit cancer hot spots. Proc Natl Acad Sci U S A (1996) 1.42
Complete cytolysis and neonatal lethality in keratin 5 knockout mice reveal its fundamental role in skin integrity and in epidermolysis bullosa simplex. Mol Biol Cell (2001) 1.41
New best estimates for radionuclide solid-liquid distribution coefficients in soils. Part 3: miscellany of radionuclides (Cd, Co, Ni, Zn, I, Se, Sb, Pu, Am, and others). J Environ Radioact (2008) 1.40
Olfactory marker protein gene: its structure and olfactory neuron-specific expression in transgenic mice. Proc Natl Acad Sci U S A (1989) 1.37
Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin. Nucleic Acids Res (1979) 1.36
Exosomes released during reticulocyte maturation bind to fibronectin via integrin alpha4beta1. Eur J Biochem (2000) 1.35
DNA sequence analysis of prm-mutations of coliphage lambda. Gene (1980) 1.34
Mapping and characterization of the interaction domains of human papillomavirus type 16 E1 and E2 proteins. J Virol (1997) 1.32
Precision top-quark mass measurement at CDF. Phys Rev Lett (2012) 1.30
A genetic strategy to eliminate self-activator baits prior to high-throughput yeast two-hybrid screens. Genome Res (1999) 1.28
Direct interaction of Smn with dp103, a putative RNA helicase: a role for Smn in transcription regulation? Hum Mol Genet (2000) 1.26
Measurement of the cross section for prompt isolated diphoton production using the full CDF run II data sample. Phys Rev Lett (2013) 1.22
Truncated WT1 mutants alter the subnuclear localization of the wild-type protein. Proc Natl Acad Sci U S A (1995) 1.22
Chemical and physical properties of lipopolysaccharide of Yersinia pestis. J Bacteriol (1974) 1.21
Cloning and partial DNA sequencing of two new human papillomavirus types associated with condylomas and low-grade cervical neoplasia. J Virol (1989) 1.21
Prediction of trace element mobility in contaminated soils by sequential extraction. J Environ Qual (2003) 1.19
Possible prognostic significance of human papillomavirus type in cervical cancer. Gynecol Oncol (1988) 1.18
LIN-5 is a novel component of the spindle apparatus required for chromosome segregation and cleavage plane specification in Caenorhabditis elegans. J Cell Biol (2000) 1.17
Decay-accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) are released within exosomes during In vitro maturation of reticulocytes. Blood (1998) 1.17
Correlation of cellular atypia and human papillomavirus deoxyribonucleic acid sequences in exfoliated cells of the uterine cervix. Obstet Gynecol (1986) 1.16
Differential effects of alpha-beta-methylene ATP on responses to nerve stimulation in SHR and WKY tail arteries. Naunyn Schmiedebergs Arch Pharmacol (1986) 1.15
N6,O2'-dibutyryl cycle AMP and glucose regulate the amount of messenger RNA coding for hepatic phosphoenolpyruvate carboxykinase (GTP). J Biol Chem (1982) 1.13
Measurement of the WW + WZ production cross section using the lepton + jets final state at CDF II. Phys Rev Lett (2010) 1.12
A novel human DnaJ protein, hTid-1, a homolog of the Drosophila tumor suppressor protein Tid56, can interact with the human papillomavirus type 16 E7 oncoprotein. Virology (1998) 1.12
Exocrine pancreatic disorders in transsgenic mice expressing human keratin 8. J Clin Invest (1999) 1.11
Suppression of the nonsense mutation in homozygous beta 0 thalassaemia. Nature (1979) 1.11
First observation of the decay Bs0-->Ds-Ds+ and measurement of its branching ratio. Phys Rev Lett (2008) 1.11
Trastuzumab-related cardiotoxicity in the elderly: a role for cardiovascular risk factors. Ann Oncol (2011) 1.10
Adverse events risk associated with bevacizumab addition to breast cancer chemotherapy: a meta-analysis. Ann Oncol (2011) 1.09
Telecare in a structured therapeutic education programme addressed to patients with type 1 diabetes and poor metabolic control. Diabetes Res Clin Pract (2006) 1.08
Evidence for a particle produced in association with weak bosons and decaying to a bottom-antibottom quark pair in higgs boson searches at the tevatron. Phys Rev Lett (2012) 1.08
Human papillomavirus type 52: a new virus associated with cervical neoplasia. J Gen Virol (1988) 1.08
A Sos-derived peptidimer blocks the Ras signaling pathway by binding both Grb2 SH3 domains and displays antiproliferative activity. FASEB J (1999) 1.07
Precise measurement of the W-boson mass with the CDF II detector. Phys Rev Lett (2012) 1.06
A soil sampling strategy for mapping trace element concentrations in a test area. Sci Total Environ (2001) 1.06
Müllerianosis of the urinary bladder: clinical and immunohistochemical findings. Histopathology (1998) 1.05
Synthesis and biological evaluation of new diisocyanide- and triisocyanide-99mTc complexes. Appl Radiat Isot (1996) 1.04
Separation and translation of the mRNAs coding for and chains of rabbit globin. Proc Natl Acad Sci U S A (1972) 1.04