Published in Nat Biotechnol on December 01, 2000
In vitro and in vivo gene therapy vector evolution via multispecies interbreeding and retargeting of adeno-associated viruses. J Virol (2008) 3.79
Laboratory-directed protein evolution. Microbiol Mol Biol Rev (2005) 1.69
Molecular engineering of viral gene delivery vehicles. Annu Rev Biomed Eng (2008) 1.28
The conversion of 3' UTRs into coding regions. Mol Biol Evol (2006) 1.24
Creating multiple-crossover DNA libraries independent of sequence identity. Proc Natl Acad Sci U S A (2001) 1.18
Predicting crossover generation in DNA shuffling. Proc Natl Acad Sci U S A (2001) 1.12
Transgenic nonhuman primates for neurodegenerative diseases. Reprod Biol Endocrinol (2004) 1.06
Gene therapy for the nervous system: challenges and new strategies. Neurotherapeutics (2014) 0.98
Evolution of a human immunodeficiency virus type 1 variant with enhanced replication in pig-tailed macaque cells by DNA shuffling. J Virol (2002) 0.90
Revealing biases inherent in recombination protocols. BMC Biotechnol (2007) 0.87
eCodonOpt: a systematic computational framework for optimizing codon usage in directed evolution experiments. Nucleic Acids Res (2002) 0.86
Computational and experimental analysis of DNA shuffling. Proc Natl Acad Sci U S A (2003) 0.83
Optimized expression and specific activity of IL-12 by directed molecular evolution. Proc Natl Acad Sci U S A (2003) 0.83
Synthetic virology: engineering viruses for gene delivery. Wiley Interdiscip Rev Nanomed Nanobiotechnol (2014) 0.81
Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors. Gene Ther (2013) 0.81
The rarity of gene shuffling in conserved genes. Genome Biol (2005) 0.79
SCHEMA computational design of virus capsid chimeras: calibrating how genome packaging, protection, and transduction correlate with calculated structural disruption. ACS Synth Biol (2013) 0.76
Reviving the RNA World: An Insight into the Appearance of RNA Methyltransferases. Front Genet (2016) 0.76
Antisense gene silencing: therapy for neurodegenerative disorders? Genes (Basel) (2013) 0.75
Viral vectors do the DNA shuffle. Nat Biotechnol (2000) 0.75
Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat Biotechnol (1996) 7.94
A simple and efficient non-organic procedure for the isolation of genomic DNA from blood. Nucleic Acids Res (1989) 5.27
Search for cross-species transmission of porcine endogenous retrovirus in patients treated with living pig tissue. The XEN 111 Study Group. Science (1999) 4.44
DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature (1998) 4.21
Barttin is a Cl- channel beta-subunit crucial for renal Cl- reabsorption and inner ear K+ secretion. Nature (2001) 3.69
A novel gene encoding an SH3 domain protein is mutated in nephronophthisis type 1. Nat Genet (1997) 2.88
Mutation of BSND causes Bartter syndrome with sensorineural deafness and kidney failure. Nat Genet (2001) 2.87
A pattern of accumulation of a somatic deletion of mitochondrial DNA in aging human tissues. Proc Natl Acad Sci U S A (1992) 2.83
Real-time fluorescence detection of a single DNA molecule. Biotechniques (1998) 2.47
Predicting the emergence of antibiotic resistance by directed evolution and structural analysis. Nat Struct Biol (2001) 2.45
Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice. Nature (2000) 2.41
Therapy of malignant brain tumors by intratumoral implantation of retroviral vector-producing cells. Nat Med (1997) 2.39
Identification of a subdomain in the Moloney murine leukemia virus envelope protein involved in receptor binding. J Virol (1996) 2.12
Molecular evolution of an arsenate detoxification pathway by DNA shuffling. Nat Biotechnol (1997) 2.05
Improved methods of retroviral vector transduction and production for gene therapy. Hum Gene Ther (1994) 1.95
Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening. Proc Natl Acad Sci U S A (1997) 1.71
Metallothionein gene duplications and metal tolerance in natural populations of Drosophila melanogaster. Genetics (1987) 1.70
Molecular breeding of viruses. Nat Genet (2000) 1.63
Regulated expression at high copy number allows production of a growth-inhibitory oncogene product in Drosophila Schneider cells. Genes Dev (1989) 1.56
Nucleotide sequence and expression of a Drosophila metallothionein. J Biol Chem (1985) 1.54
Protein evolution by molecular breeding. Curr Opin Chem Biol (1999) 1.44
Molecular and cytogenetic characterization of a metallothionein gene of Drosophila. Genetics (1986) 1.43
Directed evolution of proteins by exon shuffling. Nat Biotechnol (2001) 1.41
Molecular genetics of nephronophthisis and medullary cystic kidney disease. J Am Soc Nephrol (2000) 1.37
DNA shuffling of subgenomic sequences of subtilisin. Nat Biotechnol (1999) 1.36
A DNA segment controlling metal-regulated expression of the Drosophila melanogaster metallothionein gene Mtn. Mol Cell Biol (1987) 1.34
Purkinje cell degeneration associated with erythroid ankyrin deficiency in nb/nb mice. J Cell Biol (1991) 1.32
Engraftment of hematopoietic progenitor cells transduced with the Fanconi anemia group C gene (FANCC). Hum Gene Ther (1999) 1.28
Primary structure and domain organization of human alpha and beta adducin. J Cell Biol (1991) 1.27
Specific 33-residue repeat(s) of erythrocyte ankyrin associate with the anion exchanger. J Biol Chem (1991) 1.26
Identification of the block in targeted retroviral-mediated gene transfer. Proc Natl Acad Sci U S A (1999) 1.25
Retroviral transfer of the glucocerebrosidase gene into CD34+ cells from patients with Gaucher disease: in vivo detection of transduced cells without myeloablation. Hum Gene Ther (1998) 1.25
Construction and evolution of antibody-phage libraries by DNA shuffling. Nat Med (1996) 1.24
Structure and expression of a tandem duplication of the Drosophila metallothionein gene. Proc Natl Acad Sci U S A (1986) 1.21
Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes. Chem Biol (2001) 1.20
Improving the catalytic activity of a thermophilic enzyme at low temperatures. Biochemistry (2000) 1.15
Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling. Nat Biotechnol (1999) 1.13
A new 440-kD isoform is the major ankyrin in neonatal rat brain. J Cell Biol (1991) 1.13
The glycoprotein products of varicella-zoster virus gene 14 and their defective accumulation in a vaccine strain (Oka). J Virol (1990) 1.13
Identification of 11 novel mutations in eight BBS genes by high-resolution homozygosity mapping. J Med Genet (2009) 1.13
Fresh non-fruit latex of Carica papaya contains papain, multiple forms of chymopapain A and papaya proteinase omega. Biochem J (1985) 1.12
Children with ocular motor apraxia type Cogan carry deletions in the gene (NPHP1) for juvenile nephronophthisis. J Pediatr (2000) 1.11
Evolution of a cytokine using DNA family shuffling. Nat Biotechnol (1999) 1.11
10(20)-fold aptamer library amplification without gel purification. Nucleic Acids Res (1993) 1.07
Applications of DNA shuffling to pharmaceuticals and vaccines. Curr Opin Biotechnol (1997) 1.07
Combinatorial multiple cassette mutagenesis creates all the permutations of mutant and wild-type sequences. Biotechniques (1995) 1.06
The evolution of molecular computation. Science (1995) 1.05
Establishing an algorithm for molecular genetic diagnostics in 127 families with juvenile nephronophthisis. Kidney Int (2001) 1.04
The Uromodulin C744G mutation causes MCKD2 and FJHN in children and adults and may be due to a possible founder effect. Kidney Int (2007) 1.01
A gene locus for branchio-otic syndrome maps to chromosome 14q21.3-q24.3. J Med Genet (2003) 1.00
A possible role for glycoprotein gpV in the pathogenesis of varicella-zoster virus. Adv Exp Med Biol (1990) 0.95
Efficacy of antiretroviral agents against murine replication-competent retrovirus infection in human cells. J Virol (1999) 0.94
Phase I safety and immunogenicity evaluations of an alphavirus replicon HIV-1 subtype C gag vaccine in healthy HIV-1-uninfected adults. Clin Vaccine Immunol (2012) 0.89
Animal and human bites as an emergency. Health Bull (Edinb) (1983) 0.89
Analysis of orbital T cells in thyroid-associated ophthalmopathy. Clin Exp Immunol (1998) 0.88
Glycosaminoglycans in thyroid-associated ophthalmopathy. Autoimmunity (1992) 0.87
Antiretroviral agents inhibit infection of human cells by porcine endogenous retroviruses. Antimicrob Agents Chemother (2000) 0.86
Improved strategy for molecular genetic diagnostics in juvenile nephronophthisis. Am J Kidney Dis (2001) 0.86
Differential alterations in tachykinin NK2 receptors in isolated colonic circular smooth muscle in inflammatory bowel disease and idiopathic chronic constipation. Regul Pept (2001) 0.86
An improved retroviral vector encoding the herpes simplex virus thymidine kinase gene increases antitumor efficacy in vivo. Cancer Gene Ther (1995) 0.86
Glycosaminoglycans in autoimmunity. Clin Exp Rheumatol (1996) 0.84
Does antibiotic prophylaxis at the time of catheter insertion reduce the incidence of catheter-related sepsis in intravenous nutrition? J Hosp Infect (1985) 0.83
Diabetic microangiopathy and urinary glycosaminoglycans. Exp Clin Endocrinol Diabetes (1997) 0.83
Molecular breeding: the natural approach to protein design. Adv Protein Chem (2000) 0.83
Rates of small intestinal mucosal protein synthesis in human jejunum and ileum. Am J Physiol (1999) 0.83
Affinity selective isolation of ligands from peptide libraries through display on a lac repressor "headpiece dimer". J Mol Biol (1996) 0.83
DNA shuffling and vaccines. Curr Opin Mol Ther (2001) 0.83
Refinement of the gene locus for autosomal dominant medullary cystic kidney disease type 1 (MCKD1) and construction of a physical and partial transcriptional map of the region. Genomics (2001) 0.82
Diversity of ankyrins in the brain. Biochem Soc Trans (1991) 0.82
Molecular cloning of the critical region for glomerulopathy with fibronectin deposits (GFND) and evaluation of candidate genes. Genomics (2000) 0.81
HPLC glycosaminoglycan analysis in patients with Graves' disease. Clin Sci (Lond) (1997) 0.81
Molecular evaluation of biopsy and autopsy specimens from patients receiving in vivo retroviral gene therapy. Hum Gene Ther (1999) 0.81
Biosafety monitoring of patients receiving intracerebral injections of murine retroviral vector producer cells. Hum Gene Ther (1998) 0.80
Reassembly of a fimbrial hemagglutinin from Pseudomonas solanacearum after purification of the subunit by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Appl Environ Microbiol (1985) 0.80
The metallothionein gene of Drosophila. Experientia Suppl (1987) 0.79
Cellular immunity and retrobulbar fibroblasts in Graves' ophthalmopathy. Thyroid (1994) 0.79
In vitro analysis of transformation potential associated with retroviral vector insertions. Hum Gene Ther (1999) 0.79
Influence of prior events on cognitive judgments in amnesia. J Exp Psychol Learn Mem Cogn (1992) 0.78
Impaired anal sensation and early diabetic faecal incontinence. Diabet Med (1991) 0.77
Muscle creatine content in rats given repeated large doses of nicotinamide: effects of dietary methionine, choline, carnitine, and other supplements. J Nutr (1986) 0.77
Effects of heavy metals on Drosophila larvae and a metallothionein cDNA. Environ Health Perspect (1986) 0.76
Chromosomal characterization of MRC-5 cell banks utilizing G-banding technique. Dev Biol Stand (1998) 0.75
Colorless green ideas... Nat Biotechnol (1999) 0.75
[On the effect of CO2 partial pressure of room air on human sleeping behavior]. Z Gesamte Hyg (1967) 0.75
[EEG-activity patterns in visual information processing: characteristics, variance-analytical deliminiation, discriminance-analytical determination of undispensable variables and scale level]. Acta Biol Med Ger (1979) 0.75
[Event-related appearance of alpha-wave groups in the EEG during solution of periodically projected arithmetic tasks]. Z Psychol Z Angew Psychol (1979) 0.75
[Activation-dependent characteristics of the electroencephalogram during visual information--processing: Results of an automatic interval analysis (author's transl)]. Act Nerv Super (Praha) (1976) 0.75
[The mineral content of cattle hair: proportions in young bulls and suckling calves]. Wien Tierarztl Monatsschr (1966) 0.75
[Circulation load test and quality of meat in fattening pigs of known genetic origin]. Arch Exp Veterinarmed (1976) 0.75
[The influence of various performance requirements on time estimation, visual discrimination reaction time, degree of alertness, spontaneous EEG activity and heart rate]. Z Gesamte Hyg (1976) 0.75
[Activation-dependent characteristics of the electroencephalogram during visual information processing: II Results of an automatic amplitude analysis based on momentary values of signal voltage (author's transl)]. Act Nerv Super (Praha) (1978) 0.75
[Activation dependency, requirement specificity and temporal behavior of physiologic and psychologic variables in stepwise increased performance requirements with regard to the Concentration-Achievement Test]. Z Psychol Z Angew Psychol (1977) 0.75
[Performance dependent lysine requirement of fattening pigs. 3. Effect of amino acid and energy intake on fat, protein and lysine deposition of swine]. Arch Tierernahr (1978) 0.75
[Repeated characterization, analysis of variance limitation and discrimination analysis classification of EEG-activity patterns in human sleep]. Acta Biol Med Ger (1977) 0.75
[Performance-related lysine requirement of fattening pigs. 4. Effect of energy and amino acid intakes on composition of gain and derivation of lysine requirement]. Arch Tierernahr (1978) 0.75