Published in J Clin Microbiol on October 01, 2003
Reagent and laboratory contamination can critically impact sequence-based microbiome analyses. BMC Biol (2014) 4.39
An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications. PLoS One (2010) 2.07
Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification. J Clin Microbiol (2006) 1.49
High-fat maternal diet during pregnancy persistently alters the offspring microbiome in a primate model. Nat Commun (2014) 1.40
Reduced PCR sensitivity due to impaired DNA recovery with the MagNA Pure LC total nucleic acid isolation kit. J Clin Microbiol (2005) 1.33
Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria. PLoS One (2009) 1.00
Gram stain-specific-probe-based real-time PCR for diagnosis and discrimination of bacterial neonatal sepsis. J Clin Microbiol (2008) 0.96
Multiplex real-time PCR assay for rapid detection of methicillin-resistant staphylococci directly from positive blood cultures. J Clin Microbiol (2014) 0.89
A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination. J Clin Microbiol (2011) 0.85
Implementation of Bacterial Detection Methods into Blood Donor Screening - Overview of Different Technologies. Transfus Med Hemother (2011) 0.81
Removal of Contaminant DNA by Combined UV-EMA Treatment Allows Low Copy Number Detection of Clinically Relevant Bacteria Using Pan-Bacterial Real-Time PCR. PLoS One (2015) 0.78
Real-time PCR TaqMan assay for rapid screening of bloodstream infection. Ann Clin Microbiol Antimicrob (2014) 0.78
A duplex PCR-based assay for measuring the amount of bacterial contamination in a nucleic acid extract from a culture of free-living protists. PLoS One (2013) 0.77
Evaluation of an ethidium monoazide-enhanced 16S rDNA real-time polymerase chain reaction assay for bacterial screening of platelet concentrates and comparison with automated culture. Transfusion (2013) 0.77
The effect of rifaximin on gut flora and Staphylococcus resistance. Dig Dis Sci (2013) 0.76
Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis. J Pathog (2012) 0.76
Usefulness of Multiplex Real-Time PCR for Simultaneous Pathogen Detection and Resistance Profiling of Staphylococcal Bacteremia. Biomed Res Int (2016) 0.75
An Internal Reference Control Duplex Real-Time Polymerase Chain Reaction Assay for Detecting Bacterial Contamination in Blood Products. PLoS One (2015) 0.75
Automated Broad-Range Molecular Detection of Bacteria in Clinical Samples. J Clin Microbiol (2016) 0.75
Evaluation of a broad range real-time polymerase chain reaction (RT-PCR) assay for the diagnosis of septic synovitis in horses. Can J Vet Res (2013) 0.75
Massively parallel digital high resolution melt for rapid and absolutely quantitative sequence profiling. Sci Rep (2017) 0.75
Rapid and simple method for purification of nucleic acids. J Clin Microbiol (1990) 44.73
Avoiding false positives with PCR. Nature (1989) 30.66
Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology (2002) 9.27
Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. J Clin Microbiol (2000) 4.44
Frequent contamination of Taq polymerase with DNA. Clin Chem (1990) 2.23
Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens. J Clin Microbiol (2002) 2.12
Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology. J Clin Microbiol (2002) 2.08
Fully automated nucleic acid extraction: MagNA Pure LC. Clin Chem (2001) 1.69
Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion. J Clin Microbiol (1999) 1.63
Decontamination of polymerase chain reaction reagents for detection of low concentrations of 16S rRNA genes. Mol Biotechnol (1997) 1.34
Detection of bacteraemia in critically ill patients using 16S rDNA polymerase chain reaction and DNA sequencing. Intensive Care Med (2001) 1.10
Blood product-associated bacterial sepsis. Transfus Med Rev (1991) 0.97
Bacterial contamination of platelet concentrates: incidence, significance, and prevention. Semin Hematol (2001) 0.95
Approaches to the detection of bacterial contamination in cellular blood products. Transfus Med Rev (1999) 0.94
Contamination management of broad-range ribosomal DNA PCR: where is the evidence? J Clin Microbiol (2002) 0.93
Preventing surgical-site infections in nasal carriers of Staphylococcus aureus. N Engl J Med (2010) 8.50
Specific autoantibodies precede the symptoms of rheumatoid arthritis: a study of serial measurements in blood donors. Arthritis Rheum (2004) 7.79
Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers. Lancet (2004) 5.27
Type VII secretion--mycobacteria show the way. Nat Rev Microbiol (2007) 4.45
Meticillin-resistant Staphylococcus aureus. Lancet Infect Dis (2005) 3.18
New developments in the diagnosis of bloodstream infections. Lancet Infect Dis (2004) 3.01
Mupirocin prophylaxis against nosocomial Staphylococcus aureus infections in nonsurgical patients: a randomized study. Ann Intern Med (2004) 2.84
Cutting edge: carbohydrate profiling identifies new pathogens that interact with dendritic cell-specific ICAM-3-grabbing nonintegrin on dendritic cells. J Immunol (2003) 2.39
Alarmingly poor performance in Chlamydia trachomatis point-of-care testing. Sex Transm Infect (2010) 2.16
Helicobacter pylori modulates the T helper cell 1/T helper cell 2 balance through phase-variable interaction between lipopolysaccharide and DC-SIGN. J Exp Med (2004) 2.08
Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR. J Clin Microbiol (2002) 2.06
Resistance to selective decontamination: the jury is still out. Lancet Infect Dis (2013) 2.03
Zebrafish embryos as a model host for the real time analysis of Salmonella typhimurium infections. Cell Microbiol (2003) 1.98
A specific secretion system mediates PPE41 transport in pathogenic mycobacteria. Mol Microbiol (2006) 1.97
Methicillin-resistant Staphylococcus aureus in meat products, the Netherlands. Emerg Infect Dis (2007) 1.94
Increased levels of C-reactive protein in serum from blood donors before the onset of rheumatoid arthritis. Arthritis Rheum (2004) 1.94
Vitamin D deficiency does not increase the risk of rheumatoid arthritis: comment on the article by Merlino et al. Arthritis Rheum (2006) 1.79
A star with stripes: zebrafish as an infection model. Trends Microbiol (2004) 1.76
Medical-grade honey kills antibiotic-resistant bacteria in vitro and eradicates skin colonization. Clin Infect Dis (2008) 1.75
Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice. J Clin Microbiol (2006) 1.74
How honey kills bacteria. FASEB J (2010) 1.71
Extended-spectrum β-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors. Clin Infect Dis (2012) 1.69
The Helicobacter pylori homologue of the ferric uptake regulator is involved in acid resistance. Infect Immun (2002) 1.65
Retreatment with telaprevir combination therapy in hepatitis C patients with well-characterized prior treatment response. Hepatology (2011) 1.62
Widespread transfer of resistance genes between bacterial species in an intensive care unit: implications for hospital epidemiology. J Clin Microbiol (2005) 1.60
Periodontal pathogens: a quantitative comparison of anaerobic culture and real-time PCR. FEMS Immunol Med Microbiol (2005) 1.52
Antiviral effect of peginterferon alfa-2b and alfa-2a compared. J Hepatol (2006) 1.42
Emergence of multidrug-resistant Gram-negative bacteria during selective decontamination of the digestive tract on an intensive care unit. J Antimicrob Chemother (2006) 1.40
Antiviral efficacy of NS3-serine protease inhibitor BILN-2061 in patients with chronic genotype 2 and 3 hepatitis C. Hepatology (2005) 1.38
Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction. J Periodontol (2007) 1.36
General secretion signal for the mycobacterial type VII secretion pathway. Proc Natl Acad Sci U S A (2012) 1.34
The ESX-5 secretion system of Mycobacterium marinum modulates the macrophage response. J Immunol (2008) 1.33
Zebrafish embryo screen for mycobacterial genes involved in the initiation of granuloma formation reveals a newly identified ESX-1 component. Dis Model Mech (2011) 1.32
Role of healthcare workers in outbreaks of methicillin-resistant Staphylococcus aureus: a 10-year evaluation from a Dutch university hospital. Infect Control Hosp Epidemiol (2003) 1.30
Mycobacterium marinum strains can be divided into two distinct types based on genetic diversity and virulence. Infect Immun (2004) 1.29
Emergence of colistin resistance in Enterobacteriaceae after the introduction of selective digestive tract decontamination in an intensive care unit. Antimicrob Agents Chemother (2013) 1.29
Associations between Staphylococcus aureus Genotype, Infection, and In-Hospital Mortality: A Nested Case-Control Study. J Infect Dis (2005) 1.29
The role of the Ferric Uptake Regulator (Fur) in regulation of Helicobacter pylori iron uptake. Helicobacter (2002) 1.29
Nosocomial spread of a Staphylococcus capitis strain with heteroresistance to vancomycin in a neonatal intensive care unit. J Clin Microbiol (2002) 1.27
The human microbiome as a reservoir of antimicrobial resistance. Front Microbiol (2013) 1.27
Streptococcus pneumoniae DNA load in blood as a marker of infection in patients with community-acquired pneumonia. J Clin Microbiol (2009) 1.25
Comparison of real-time PCR and culture for detection of Porphyromonas gingivalis in subgingival plaque samples. J Clin Microbiol (2003) 1.25
Transfusion-transmitted infectious diseases. Biologicals (2009) 1.25
Transcriptional phase variation of a type III restriction-modification system in Helicobacter pylori. J Bacteriol (2002) 1.23
Enrichment broth improved detection of extended-spectrum-beta-lactamase-producing bacteria in throat and rectal surveillance cultures of samples from patients in intensive care units. J Clin Microbiol (2009) 1.22
Single-nucleotide-polymorphism genotyping of Coxiella burnetii during a Q fever outbreak in The Netherlands. Appl Environ Microbiol (2011) 1.21
Identification of mycobacterial alpha-glucan as a novel ligand for DC-SIGN: involvement of mycobacterial capsular polysaccharides in host immune modulation. J Immunol (2009) 1.17
Two major medicinal honeys have different mechanisms of bactericidal activity. PLoS One (2011) 1.17
Molecular mimicry between Helicobacter pylori antigens and H+, K+ --adenosine triphosphatase in human gastric autoimmunity. J Exp Med (2003) 1.14
HTLV-I/II prevalence in different geographic locations. Transfus Med Rev (2004) 1.14
Quantitative detection of Staphylococcus aureus and Enterococcus faecalis DNA in blood to diagnose bacteremia in patients in the intensive care unit. J Clin Microbiol (2007) 1.13
Prevalence of Chlamydophila psittaci in fecal droppings from feral pigeons in Amsterdam, The Netherlands. Appl Environ Microbiol (2006) 1.12
Outbreak of vancomycin-resistant Enterococcus faecium in a haematology unit: risk factor assessment and successful control of the epidemic. Br J Haematol (2002) 1.11
Using a dog's superior olfactory sensitivity to identify Clostridium difficile in stools and patients: proof of principle study. BMJ (2012) 1.08
Genotyping of Chlamydophila psittaci in human samples. Emerg Infect Dis (2006) 1.08
An outbreak of psittacosis due to Chlamydophila psittaci genotype A in a veterinary teaching hospital. J Med Microbiol (2006) 1.07
Analysis of multiple single nucleotide polymorphisms (SNP) on DNA traces from plasma and dried blood samples. J Immunol Methods (2007) 1.06
Contact isolation is a risk factor for venous thromboembolism in trauma patients. J Trauma Acute Care Surg (2016) 1.06
Identification of the mycobacterial carbohydrate structure that binds the C-type lectins DC-SIGN, L-SIGN and SIGNR1. Immunobiology (2004) 1.04
Hospital-related determinants for surgical-site infection following hip arthroplasty. Infect Control Hosp Epidemiol (2005) 1.03
Characterization of five novel Pseudomonas aeruginosa cell-surface signalling systems. Mol Microbiol (2007) 1.03
Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay. J Clin Microbiol (2006) 1.02
Characterisation of carbapenem-resistant Acinetobacter baumannii outbreak strains producing OXA-58 in Turkey. Int J Antimicrob Agents (2010) 1.02
A case of New Delhi metallo-β-lactamase 1 (NDM-1)-producing Klebsiella pneumoniae with putative secondary transmission from the Balkan region in the Netherlands. Antimicrob Agents Chemother (2012) 1.01
Genetic markers for Clostridium difficile lineages linked to hypervirulence. Microbiology (2011) 0.97
Cellular immune responses during high-dose interferon-alpha induction therapy for hepatitis C virus infection. J Infect Dis (2009) 0.97
Detection of bacteria in platelet concentrates: comparison of broad-range real-time 16S rDNA polymerase chain reaction and automated culturing. Transfusion (2005) 0.96
High incidence of type 1 diabetes mellitus during or shortly after treatment with pegylated interferon alpha for chronic hepatitis C virus infection. Liver Int (2007) 0.95
Detection of bacterial DNA in blood samples from febrile patients: underestimated infection or emerging contamination? FEMS Immunol Med Microbiol (2004) 0.95
Characterization of the integrated filamentous phage Pf5 and its involvement in small-colony formation. Microbiology (2007) 0.95
The additional value of real-time PCR in the quantitative detection of periodontal pathogens. J Clin Periodontol (2006) 0.95
Reduced risk of surgical site infections through surveillance in a network. Int J Qual Health Care (2006) 0.94
Non-invasive evaluation of liver fibrosis: a comparison of ultrasound-based transient elastography and MR elastography in patients with viral hepatitis B and C. Eur Radiol (2013) 0.94
Role of phosphatidylinositol mannosides in the interaction between mycobacteria and DC-SIGN. Infect Immun (2009) 0.93
Storage of platelets in additive solution for up to 12 days with maintenance of good in-vitro quality. Transfusion (2004) 0.92
Molecular patchwork: Chromosomal recombination between two Helicobacter pylori strains during natural colonization. Infect Immun (2003) 0.91
Improved risk adjustment for comparison of surgical site infection rates. Infect Control Hosp Epidemiol (2006) 0.91
Treatment with clarithromycin prior to coronary artery bypass graft surgery does not prevent subsequent cardiac events. Clin Infect Dis (2005) 0.90
High rates of antimicrobial drug resistance gene acquisition after international travel, The Netherlands. Emerg Infect Dis (2014) 0.90
Functional characterization of the competence protein DprA/Smf in Escherichia coli. FEMS Microbiol Lett (2006) 0.89
Characteristics of clinical Helicobacter pylori strains from Ecuador. J Antimicrob Chemother (2003) 0.88