Published in Trends Biotechnol on February 01, 2004
Engineering anatomically shaped human bone grafts. Proc Natl Acad Sci U S A (2009) 2.37
Scaffold-free vascular tissue engineering using bioprinting. Biomaterials (2009) 2.32
Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng (2012) 2.27
Effects of initial seeding density and fluid perfusion rate on formation of tissue-engineered bone. Tissue Eng Part A (2008) 1.70
Layer by layer three-dimensional tissue epitaxy by cell-laden hydrogel droplets. Tissue Eng Part C Methods (2010) 1.46
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Hypoxia and stem cell-based engineering of mesenchymal tissues. Biotechnol Prog (2009) 1.36
Generation of cerebral organoids from human pluripotent stem cells. Nat Protoc (2014) 1.25
Cartilage tissue engineering and bioreactor systems for the cultivation and stimulation of chondrocytes. Eur Biophys J (2007) 1.25
Method to analyze three-dimensional cell distribution and infiltration in degradable scaffolds. Tissue Eng Part C Methods (2008) 1.21
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Spatial regulation of human mesenchymal stem cell differentiation in engineered osteochondral constructs: effects of pre-differentiation, soluble factors and medium perfusion. Osteoarthritis Cartilage (2010) 1.09
Mechanical stretching for tissue engineering: two-dimensional and three-dimensional constructs. Tissue Eng Part B Rev (2012) 1.04
Three-dimensional in vitro tumor models for cancer research and drug evaluation. Biotechnol Adv (2014) 1.03
Mathematical modelling of engineered tissue growth using a multiphase porous flow mixture theory. J Math Biol (2006) 1.02
Quantification of dynamic morphological drug responses in 3D organotypic cell cultures by automated image analysis. PLoS One (2014) 1.00
Concise review: optimizing expansion of bone marrow mesenchymal stem/stromal cells for clinical applications. Stem Cells Transl Med (2014) 1.00
Imaging engineered tissues using structural and functional optical coherence tomography. J Biophotonics (2009) 0.99
Human mesenchymal stem cell position within scaffolds influences cell fate during dynamic culture. Biotechnol Bioeng (2012) 0.98
Trends in tissue engineering for blood vessels. J Biomed Biotechnol (2012) 0.94
Magnetic nanoparticle-based approaches to locally target therapy and enhance tissue regeneration in vivo. Nanomedicine (Lond) (2012) 0.93
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The role of perfusion bioreactors in bone tissue engineering. Biomatter (2013) 0.92
Tissue-engineered heart valve: future of cardiac surgery. World J Surg (2012) 0.90
Adipose tissue as a stem cell source for musculoskeletal regeneration. Front Biosci (Schol Ed) (2011) 0.90
Finite element method (FEM), mechanobiology and biomimetic scaffolds in bone tissue engineering. Int J Biol Sci (2011) 0.90
Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds. J Biomed Opt (2009) 0.89
The role of bioreactors in tissue engineering for musculoskeletal applications. Open Orthop J (2011) 0.87
Flow characterization of a spinner flask for induced pluripotent stem cell culture application. PLoS One (2014) 0.87
Cell-nanofiber-based cartilage tissue engineering using improved cell seeding, growth factor, and bioreactor technologies. Tissue Eng Part A (2008) 0.87
A novel bioreactor for the dynamic stimulation and mechanical evaluation of multiple tissue-engineered constructs. Tissue Eng Part C Methods (2010) 0.86
Strategies for improving the physiological relevance of human engineered tissues. Trends Biotechnol (2015) 0.85
Strategic design and fabrication of engineered scaffolds for articular cartilage repair. J Funct Biomater (2012) 0.85
Introduction to cell-hydrogel mechanosensing. Interface Focus (2014) 0.84
Fabrication and application of nanofibrous scaffolds in tissue engineering. Curr Protoc Cell Biol (2009) 0.83
Roles of adherent myogenic cells and dynamic culture in engineered muscle function and maintenance of satellite cells. Biomaterials (2014) 0.83
Use of flow, electrical, and mechanical stimulation to promote engineering of striated muscles. Ann Biomed Eng (2013) 0.83
Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects. Cell Stress Chaperones (2012) 0.82
Strategies for tissue engineering cardiac constructs to affect functional repair following myocardial infarction. J Cardiovasc Transl Res (2011) 0.82
Nano-regenerative medicine towards clinical outcome of stem cell and tissue engineering in humans. J Cell Mol Med (2012) 0.81
Approaches to in vitro tissue regeneration with application for human disease modeling and drug development. Drug Discov Today (2014) 0.81
Simulation of cell seeding within a three-dimensional porous scaffold: a fluid-particle analysis. Tissue Eng Part C Methods (2012) 0.81
Altering the architecture of tissue engineered hypertrophic cartilaginous grafts facilitates vascularisation and accelerates mineralisation. PLoS One (2014) 0.81
Optical cell separation from three-dimensional environment in photodegradable hydrogels for pure culture techniques. Sci Rep (2014) 0.81
Application of cell and biomaterial-based tissue engineering methods in the treatment of cartilage, menisci and ligament injuries. Int Orthop (2016) 0.80
Stem cell engineered bone with calcium-phosphate coated porous titanium scaffold or silicon hydroxyapatite granules for revision total joint arthroplasty. J Mater Sci Mater Med (2014) 0.80
The interplay between tissue growth and scaffold degradation in engineered tissue constructs. J Math Biol (2012) 0.80
Modeling nutrient consumptions in large flow-through bioreactors for tissue engineering. Biotechnol Bioeng (2009) 0.80
Magnetic-based multi-layer microparticles for endothelial progenitor cell isolation, enrichment, and detachment. Biomaterials (2013) 0.80
Engineering parameters in bioreactor's design: a critical aspect in tissue engineering. Biomed Res Int (2013) 0.80
In vitro mesenchymal trilineage differentiation and extracellular matrix production by adipose and bone marrow derived adult equine multipotent stromal cells on a collagen scaffold. Stem Cell Rev (2013) 0.80
Fabrication, chemical composition change and phase evolution of biomorphic hydroxyapatite. J Mater Sci Mater Med (2008) 0.80
Printing cell-laden gelatin constructs by free-form fabrication and enzymatic protein crosslinking. Biomed Microdevices (2015) 0.79
Long term perfusion system supporting adipogenesis. Methods (2015) 0.79
Bioreactors addressing diabetes mellitus. J Diabetes Sci Technol (2014) 0.79
Rapid and cost-effective fabrication of selectively permeable calcium-alginate microfluidic device using "modified" embedded template method. Biomicrofluidics (2012) 0.79
On the genealogy of tissue engineering and regenerative medicine. Tissue Eng Part B Rev (2014) 0.79
Patterns of amino acid metabolism by proliferating human mesenchymal stem cells. Tissue Eng Part A (2011) 0.79
A differential pressure laminar flow reactor supports osteogenic differentiation and extracellular matrix formation from adipose mesenchymal stem cells in a macroporous ceramic scaffold. Biores Open Access (2012) 0.79
Skeletal tissue regeneration: where can hydrogels play a role? Int Orthop (2014) 0.78
3D Printing for Tissue Engineering. Isr J Chem (2013) 0.78
Development of in vitro 3D TissueFlex® islet model for diabetic drug efficacy testing. PLoS One (2013) 0.78
Role of culture conditions on in vitro transformation and cellular colonization of biomimetic HA-Col scaffolds. Biomatter (2013) 0.78
Microvascular guidance: a challenge to support the development of vascularised tissue engineering construct. ScientificWorldJournal (2012) 0.78
Tissue engineering of a human 3D in vitro tumor test system. J Vis Exp (2013) 0.78
Microfabricated polymeric vessel mimetics for 3-D cancer cell culture. Biomaterials (2013) 0.78
Ectopic osteogenesis of macroscopic tissue constructs assembled from human mesenchymal stem cell-laden microcarriers through in vitro perfusion culture. PLoS One (2014) 0.78
Quantitative Validation of the Presto Blue Metabolic Assay for Online Monitoring of Cell Proliferation in a 3D Perfusion Bioreactor System. Tissue Eng Part C Methods (2015) 0.78
A fiber-optic-based imaging system for nondestructive assessment of cell-seeded tissue-engineered scaffolds. Tissue Eng Part C Methods (2012) 0.78
In Vitro Cell Models for Ophthalmic Drug Development Applications. Biores Open Access (2016) 0.77
Novel pHEMA-gelatin SPHs as bone scaffolds in dynamic cultures. J Mater Sci Mater Med (2012) 0.77
A Pulsatile Bioreactor for Conditioning of Tissue-Engineered Cardiovascular Constructs under Endoscopic Visualization. J Funct Biomater (2012) 0.77
A triphasic constrained mixture model of engineered tissue formation under in vitro dynamic mechanical conditioning. Biomech Model Mechanobiol (2015) 0.77
Monitoring of metabolite gradients in tissue-engineered constructs. J R Soc Interface (2006) 0.77
Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering. Int J Nanomedicine (2015) 0.77
Distribution and Viability of Fetal and Adult Human Bone Marrow Stromal Cells in a Biaxial Rotating Vessel Bioreactor after Seeding on Polymeric 3D Additive Manufactured Scaffolds. Front Bioeng Biotechnol (2015) 0.77
Uniform beads with controllable pore sizes for biomedical applications. Small (2010) 0.77
Calcification of primary human osteoblast cultures under flow conditions using polycaprolactone scaffolds for intravascular applications. J Tissue Eng Regen Med (2011) 0.77
Bioreactor-Based Online Recovery of Human Progenitor Cells with Uncompromised Regenerative Potential: A Bone Tissue Engineering Perspective. PLoS One (2015) 0.77
Tissue Engineering Whole Bones Through Endochondral Ossification: Regenerating the Distal Phalanx. Biores Open Access (2015) 0.77
Robotic dispensing of composite scaffolds and in vitro responses of bone marrow stromal cells. J Mater Sci Mater Med (2009) 0.77
Toward 3D biomimetic models to understand the behavior of glioblastoma multiforme cells. Tissue Eng Part B Rev (2013) 0.77
Kinetic oxygen measurements by CVC96 in L-929 cell cultures. Head Face Med (2006) 0.77
Scaffolds from surgically removed kidneys as a potential source of organ transplantation. Biomed Res Int (2015) 0.76
Sequential application of steady and pulsatile medium perfusion enhanced the formation of engineered bone. Tissue Eng Part A (2013) 0.76
A Flow Perfusion Bioreactor System for Vocal Fold Tissue Engineering Applications. Tissue Eng Part C Methods (2016) 0.76
Neural Subtype Specification from Human Pluripotent Stem Cells. Cell Stem Cell (2016) 0.76
Application of an acoustofluidic perfusion bioreactor for cartilage tissue engineering. Lab Chip (2014) 0.76
Osteoblast cell response to beta-tricalcium phosphate scaffolds with controlled architecture in flow perfusion culture system. J Mater Sci Mater Med (2008) 0.76
Building a tissue in vitro from the bottom up: implications in regenerative medicine. Methodist Debakey Cardiovasc J (2013) 0.75
Morphological characteristics of cartilage-bone transitional structures in the human knee joint and CAD design of an osteochondral scaffold. Biomed Eng Online (2016) 0.75
Bioreactor-Based Tumor Tissue Engineering. Acta Naturae (2016) 0.75
The root canal system: a channel through which we can seed cells into grafts. Med Sci Monit (2014) 0.75
Anticancer Drug Camptothecin Test in 3D Hydrogel Networks with HeLa cells. Sci Rep (2017) 0.75
Effects of a Novel Inoculation Method on Cell Distribution, Mineralization, and Vascularization of Tissue-Engineered Constructs. Adv Wound Care (New Rochelle) (2016) 0.75
A PET-compatible tissue bioreactor for research, discovery, and validation of imaging biomarkers and radiopharmaceuticals: system design and proof-of-concept studies. J Nucl Med (2013) 0.75
Synthetic Calcite as a Scaffold for Osteoinductive Bone Substitutes. Ann Biomed Eng (2015) 0.75
Influence of Additive Manufactured Scaffold Architecture on the Distribution of Surface Strains and Fluid Flow Shear Stresses and Expected Osteochondral Cell Differentiation. Front Bioeng Biotechnol (2017) 0.75
Plasticity of clonal populations of dedifferentiated adult human articular chondrocytes. Arthritis Rheum (2003) 1.90
Angiogenesis in tissue engineering: breathing life into constructed tissue substitutes. Tissue Eng (2006) 1.86
Age related changes in human articular chondrocyte yield, proliferation and post-expansion chondrogenic capacity. Osteoarthritis Cartilage (2004) 1.84
IFN-alpha2a induces IP-10/CXCL10 and MIG/CXCL9 production in monocyte-derived dendritic cells and enhances their capacity to attract and stimulate CD8+ effector T cells. J Leukoc Biol (2002) 1.82
Real-time quantitative RT-PCR analysis of human bone marrow stromal cells during osteogenic differentiation in vitro. J Cell Biochem (2002) 1.66
Osteochondral tissue engineering. J Biomech (2006) 1.57
Three-dimensional culture of melanoma cells profoundly affects gene expression profile: a high density oligonucleotide array study. J Cell Physiol (2005) 1.49
A regional system for delivery of primary percutaneous coronary intervention in ST-elevation myocardial infarction: STEMI-St. Cloud. J Invasive Cardiol (2009) 1.40
Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts. Stem Cells (2005) 1.32
Tissue-engineered composites for the repair of large osteochondral defects. Arthritis Rheum (2002) 1.31
Insurance status and race represent independent predictors of undergoing laparoscopic surgery for appendicitis: secondary data analysis of 145,546 patients. J Am Coll Surg (2004) 1.27
In vitro and in vivo evaluation of differentially demineralized cancellous bone scaffolds combined with human bone marrow stromal cells for tissue engineering. Biomaterials (2005) 1.26
Interferon stimulated gene 15 constitutively produced by melanoma cells induces e-cadherin expression on human dendritic cells. Cancer Res (2002) 1.21
Cartilage tissue engineering for degenerative joint disease. Adv Drug Deliv Rev (2006) 1.18
Lubricin reduces cartilage--cartilage integration. Biorheology (2004) 1.12
High hospital volume is associated with better outcomes for breast cancer surgery: analysis of 233,247 patients. World J Surg (2005) 1.11
Cell yield, proliferation, and postexpansion differentiation capacity of human ear, nasal, and rib chondrocytes. Tissue Eng (2004) 1.09
Cancer/testis antigen expression and specific cytotoxic T lymphocyte responses in non small cell lung cancer. Int J Cancer (2007) 1.08
Anabolic and catabolic responses of human articular chondrocytes to varying oxygen percentages. Arthritis Res Ther (2010) 1.07
Effects of scaffold composition and architecture on human nasal chondrocyte redifferentiation and cartilaginous matrix deposition. Biomaterials (2005) 1.07
Bioreactor-based roadmap for the translation of tissue engineering strategies into clinical products. Trends Biotechnol (2009) 1.05
Disseminated single tumor cells as detected by real-time quantitative polymerase chain reaction represent a prognostic factor in patients undergoing surgery for colorectal cancer. Ann Surg (2002) 1.04
Precultivation of engineered human nasal cartilage enhances the mechanical properties relevant for use in facial reconstructive surgery. Ann Surg (2006) 1.03
Immunohistochemical expression of tumor antigens MAGE-A1, MAGE-A3/4, and NY-ESO-1 in cancerous and benign prostatic tissue. Prostate (2006) 1.01
Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers. BMC Cancer (2012) 1.01
Effects of in vitro preculture on in vivo development of human engineered cartilage in an ectopic model. Tissue Eng (2005) 1.01
Enhancing the biological performance of synthetic polymeric materials by decoration with engineered, decellularized extracellular matrix. Biomaterials (2012) 1.01
CD133+, CD166+CD44+, and CD24+CD44+ phenotypes fail to reliably identify cell populations with cancer stem cell functional features in established human colorectal cancer cell lines. Stem Cells Transl Med (2012) 1.00
Characterization and clinical relevance of ALDHbright populations in prostate cancer. Clin Cancer Res (2013) 0.99
Growth factors for clinical-scale expansion of human articular chondrocytes: relevance for automated bioreactor systems. Tissue Eng (2007) 0.99
High myeloperoxidase positive cell infiltration in colorectal cancer is an independent favorable prognostic factor. PLoS One (2013) 0.97
Interleukin-1β modulates endochondral ossification by human adult bone marrow stromal cells. Eur Cell Mater (2012) 0.97
High-throughput flow cytometry purification of transduced progenitors expressing defined levels of vascular endothelial growth factor induces controlled angiogenesis in vivo. Stem Cells (2010) 0.96
Differential effects of the tryptophan metabolite 3-hydroxyanthranilic acid on the proliferation of human CD8+ T cells induced by TCR triggering or homeostatic cytokines. Eur J Immunol (2006) 0.95
Characterization of highly frequent epitope-specific CD45RA+/CCR7+/- T lymphocyte responses against p53-binding domains of the human polyomavirus BK large tumor antigen in HLA-A*0201+ BKV-seropositive donors. J Transl Med (2006) 0.95
Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c. Stem Cells (2012) 0.95
Klf4 transcription factor is expressed in the cytoplasm of prostate cancer cells. Eur J Cancer (2012) 0.94
Elevated levels of circulating IL-7 and IL-15 in patients with early stage prostate cancer. J Transl Med (2011) 0.94
Three-dimensional cell culture and tissue engineering in a T-CUP (tissue culture under perfusion). Tissue Eng (2007) 0.94
Effect of three-dimensional expansion and cell seeding density on the cartilage-forming capacity of human articular chondrocytes in type II collagen sponges. J Biomed Mater Res A (2010) 0.93
Rapid induction of specific cytotoxic T lymphocytes against melanoma-associated antigens by a recombinant vaccinia virus vector expressing multiple immunodominant epitopes and costimulatory molecules in vivo. Hum Gene Ther (2002) 0.93
Intranodal immunization with a vaccinia virus encoding multiple antigenic epitopes and costimulatory molecules in metastatic melanoma. Mol Ther (2009) 0.92
FACS-purified myoblasts producing controlled VEGF levels induce safe and stable angiogenesis in chronic hind limb ischemia. J Cell Mol Med (2012) 0.89
Nonreplicating recombinant vaccinia virus expressing CD40 ligand enhances APC capacity to stimulate specific CD4+ and CD8+ T cell responses. Hum Gene Ther (2005) 0.89
Mesenchymal stromal cells induce epithelial-to-mesenchymal transition in human colorectal cancer cells through the expression of surface-bound TGF-β. Int J Cancer (2014) 0.88
VEGF over-expression in skeletal muscle induces angiogenesis by intussusception rather than sprouting. Angiogenesis (2012) 0.88
The osteogenicity of implanted engineered bone constructs is related to the density of clonogenic bone marrow stromal cells. J Tissue Eng Regen Med (2007) 0.88
Enzymatic digestion of adult human articular cartilage yields a small fraction of the total available cells. Connect Tissue Res (2003) 0.88
Induction of aberrant vascular growth, but not of normal angiogenesis, by cell-based expression of different doses of human and mouse VEGF is species-dependent. Hum Gene Ther Methods (2013) 0.87
Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing. Biomaterials (2010) 0.86
High expression of indoleamine 2,3-dioxygenase gene in prostate cancer. Eur J Cancer (2008) 0.86
Assessment of nerve damage using a novel ultrasonic device for bone cutting. J Oral Maxillofac Surg (2008) 0.86
In vitro osteogenic differentiation and in vivo bone-forming capacity of human isogenic jaw periosteal cells and bone marrow stromal cells. Ann Surg (2005) 0.85
Culture of melanoma cells in 3-dimensional architectures results in impaired immunorecognition by cytotoxic T lymphocytes specific for Melan-A/MART-1 tumor-associated antigen. Ann Surg (2005) 0.85
Differential responsiveness to IL-2, IL-7, and IL-15 common receptor gamma chain cytokines by antigen-specific peripheral blood naive or memory cytotoxic CD8+ T cells from healthy donors and melanoma patients. J Immunother (2009) 0.84
Expression of cancer/testis tumor associated antigens in cervical squamous cell carcinoma. Oncology (2003) 0.83
Use of hydrodynamic forces to engineer cartilaginous tissues resembling the non-uniform structure and function of meniscus. Biomaterials (2006) 0.83
Spatial and temporal patterns of bone formation in ectopically pre-fabricated, autologous cell-based engineered bone flaps in rabbits. J Cell Mol Med (2008) 0.83
Prognostic relevance of MAGE-A4 tumor antigen expression in transitional cell carcinoma of the urinary bladder: a tissue microarray study. Int J Cancer (2002) 0.83
Influenza virosomes enhance class I restricted CTL induction through CD4+ T cell activation. Vaccine (2004) 0.82
Differentiation-dependent up-regulation of BMP-2, TGF-beta1, and VEGF expression by FGF-2 in human bone marrow stromal cells. Plast Reconstr Surg (2005) 0.81
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Generation of human adult mesenchymal stromal/stem cells expressing defined xenogenic vascular endothelial growth factor levels by optimized transduction and flow cytometry purification. Tissue Eng Part C Methods (2011) 0.81
The ester-bonded palmitoyl side chains of Pam3CysSerLys4 lipopeptide account for its powerful adjuvanticity to HLA class I-restricted CD8+ T lymphocytes. Eur J Immunol (2003) 0.81
An RGD-restricted substrate interface is sufficient for the adhesion, growth and cartilage forming capacity of human chondrocytes. Eur Cell Mater (2010) 0.80
Osteogenic graft vascularization and bone resorption by VEGF-expressing human mesenchymal progenitors. Biomaterials (2013) 0.80
Efficient induction of tumoricidal cytotoxic T lymphocytes by HLA-A0201 restricted, melanoma associated, L(27)Melan-A/MART-1(26-35) peptide encapsulated into virosomes in vitro. Vaccine (2005) 0.79
Controlled angiogenesis in the heart by cell-based expression of specific vascular endothelial growth factor levels. Hum Gene Ther Methods (2012) 0.78
Heterologous prime-boost immunotherapy of melanoma patients with Influenza virosomes, and recombinant Vaccinia virus encoding 5 melanoma epitopes and 3 co-stimulatory molecules. A multi-centre phase I/II open labeled clinical trial. Contemp Clin Trials (2007) 0.78
Preventing vaccinia virus class-I epitopes presentation by HSV-ICP47 enhances the immunogenicity of a TAP-independent cancer vaccine epitope. Int J Cancer (2012) 0.78
Selective responsiveness to common gamma chain cytokines in peripheral blood-derived cytotoxic T lymphocytes induced by Melan-A/MART-1(27-35)targeted active specific immunotherapy. Int J Cancer (2005) 0.77
Immunohistochemical expression of tumor antigens MAGE-A1, MAGE-A3/4, and NY-ESO-1 in squamous cell carcinoma of the penis. Urology (2006) 0.77
Effect of bone sialoprotein coating of ceramic and synthetic polymer materials on in vitro osteogenic cell differentiation and in vivo bone formation. J Biomed Mater Res A (2010) 0.76
Active specific immunotherapy phase III trials for malignant melanoma: systematic analysis and critical appraisal. J Am Coll Surg (2008) 0.76
Cytotoxic T-cell induction in metastatic melanoma patients undergoing recombinant vaccinia virus-based immuno-gene therapy. Recent Results Cancer Res (2002) 0.76
Clinical applications of virosomes in cancer immunotherapy. Expert Opin Biol Ther (2006) 0.76
Designing questionnaires: healthcare survey to compare two different response scales. BMC Med Res Methodol (2014) 0.75
Ileocecal valve as substitute for the missing pyloric sphincter after partial distal gastrectomy. Ann Surg (2002) 0.75
Effects of a perfusion bioreactor activated novel bone substitute in spine fusion in sheep. Eur Spine J (2012) 0.75
Active antigen-specific immunotherapy of melanoma: from basic science to clinical investigation. World J Surg (2005) 0.75
The relationship between hospital patients' ratings of quality of care and communication. Int J Qual Health Care (2013) 0.75
Advanced liposomal vectors as cancer vaccines in melanoma immunotherapy. J Liposome Res (2006) 0.75