FRAP analysis of binding: proper and fitting.

Brd4 marks select genes on mitotic chromatin and directs postmitotic transcription. Mol Biol Cell (2009) 3.29

Accuracy and precision in quantitative fluorescence microscopy. J Cell Biol (2009) 2.98

Analysis of receptor oligomerization by FRAP microscopy. Nat Methods (2009) 2.06

A biological interpretation of transient anomalous subdiffusion. I. Qualitative model. Biophys J (2006) 2.05

Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation. Nucleic Acids Res (2007) 2.04

Evidence for a common mode of transcription factor interaction with chromatin as revealed by improved quantitative fluorescence recovery after photobleaching. Biophys J (2008) 2.04

Transcription in four dimensions: nuclear receptor-directed initiation of gene expression. EMBO Rep (2006) 1.94

Nucleolus: the fascinating nuclear body. Histochem Cell Biol (2007) 1.87

Kinetic analysis of Smad nucleocytoplasmic shuttling reveals a mechanism for transforming growth factor beta-dependent nuclear accumulation of Smads. Mol Cell Biol (2005) 1.84

Dynamics of inner kinetochore assembly and maintenance in living cells. J Cell Biol (2008) 1.84

Analysis of binding at a single spatially localized cluster of binding sites by fluorescence recovery after photobleaching. Biophys J (2006) 1.78

Imaging transcription in living cells. Annu Rev Biophys (2009) 1.76

Local zones of endoplasmic reticulum complexity confine cargo in neuronal dendrites. Cell (2012) 1.75

A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Nat Biotechnol (2011) 1.74

Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat Cell Biol (2015) 1.66

FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know? Curr Opin Cell Biol (2010) 1.64

Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy. Biophys J (2009) 1.52

In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow. Proc Natl Acad Sci U S A (2010) 1.51

A role for the juxtamembrane cytoplasm in the molecular dynamics of focal adhesions. PLoS One (2009) 1.50

Recruitment timing and dynamics of transcription factors at the Hsp70 loci in living cells. Mol Cell (2010) 1.50

Poleward transport of Eg5 by dynein-dynactin in Xenopus laevis egg extract spindles. J Cell Biol (2008) 1.49

Neuronal IP3 3-kinase is an F-actin-bundling protein: role in dendritic targeting and regulation of spine morphology. Mol Biol Cell (2009) 1.48

Forchlorfenuron alters mammalian septin assembly, organization, and dynamics. J Biol Chem (2008) 1.43

SUMO regulates the assembly and function of a cytoplasmic intermediate filament protein in C. elegans. Dev Cell (2009) 1.43

Nucleocytoplasmic distribution is required for activation of resistance by the potato NB-LRR receptor Rx1 and is balanced by its functional domains. Plant Cell (2010) 1.39

Cellular responses to DNA double-strand breaks after low-dose gamma-irradiation. Nucleic Acids Res (2009) 1.34

Complexity in transcription control at the activation domain-mediator interface. Sci Signal (2009) 1.29

A reaction-diffusion model to study RNA motion by quantitative fluorescence recovery after photobleaching. Biophys J (2007) 1.27

Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates. Biophys J (2010) 1.27

The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells. J Cell Biol (2010) 1.26

Proteins on the move: insights gained from fluorescent protein technologies. Nat Rev Mol Cell Biol (2011) 1.25

Modeling synaptic dynamics driven by receptor lateral diffusion. Biophys J (2006) 1.23

SAP102 is a highly mobile MAGUK in spines. J Neurosci (2010) 1.22

Flotillins play an essential role in Niemann-Pick C1-like 1-mediated cholesterol uptake. Proc Natl Acad Sci U S A (2010) 1.19

Morphogen transport. Development (2013) 1.16

Versatile analysis of single-molecule tracking data by comprehensive testing against Monte Carlo simulations. Biophys J (2008) 1.16

Assessing cellular efficacy of bromodomain inhibitors using fluorescence recovery after photobleaching. Epigenetics Chromatin (2014) 1.14

Alpha-actinin binding kinetics modulate cellular dynamics and force generation. Proc Natl Acad Sci U S A (2015) 1.10

Abundance and stability of complexes containing inactive G protein-coupled receptors and G proteins. FASEB J (2008) 1.09

Subunits of mitochondrial complex I exist as part of matrix- and membrane-associated subcomplexes in living cells. J Biol Chem (2008) 1.09

Endosomal transport of septin mRNA and protein indicates local translation on endosomes and is required for correct septin filamentation. EMBO Rep (2013) 1.06

Arabidopsis chromatin-associated HMGA and HMGB use different nuclear targeting signals and display highly dynamic localization within the nucleus. Plant Cell (2006) 1.05

PAR proteins diffuse freely across the anterior-posterior boundary in polarized C. elegans embryos. J Cell Biol (2011) 1.04

Fibrin networks regulate protein transport during thrombus development. PLoS Comput Biol (2013) 1.04

Live-cell imaging evidence for the ciliary transport of rod photoreceptor opsin by heterotrimeric kinesin-2. J Neurosci (2012) 1.04

Quantitative microscopy and systems biology: seeing the whole picture. Histochem Cell Biol (2008) 1.02

Nuclear proteins: finding and binding target sites in chromatin. Chromosome Res (2011) 1.01

Adenovirus E1B 55-kilodalton protein is a p53-SUMO1 E3 ligase that represses p53 and stimulates its nuclear export through interactions with promyelocytic leukemia nuclear bodies. J Virol (2010) 1.01

Minimizing the impact of photoswitching of fluorescent proteins on FRAP analysis. Biophys J (2012) 1.00

Src/FAK-mediated regulation of E-cadherin as a mechanism for controlling collective cell movement: insights from in vivo imaging. Cell Adh Migr (2011) 1.00

Uptake, distribution and diffusivity of reactive fluorophores in cells: implications toward target identification. Mol Pharm (2010) 1.00

ALS-linked mutations enlarge TDP-43-enriched neuronal RNA granules in the dendritic arbor. J Neurosci (2014) 1.00

Dynamic microtubules produce an asymmetric E-cadherin-Bazooka complex to maintain segment boundaries. J Cell Biol (2013) 1.00

Translation repressors, an RNA helicase, and developmental cues control RNP phase transitions during early development. Dev Cell (2013) 0.99

Intersection of ChIP and FLIP, genomic methods to study the dynamics of the cohesin proteins. Chromosome Res (2009) 0.98

In vivo induction of postsynaptic molecular assembly by the cell adhesion molecule Fasciclin2. J Cell Biol (2007) 0.98

Arp2/3 controls the motile behavior of N-WASP-functionalized GUVs and modulates N-WASP surface distribution by mediating transient links with actin filaments. Biophys J (2008) 0.98

A new FRAP/FRAPa method for three-dimensional diffusion measurements based on multiphoton excitation microscopy. Biophys J (2008) 0.97

Dynamic as well as stable protein interactions contribute to genome function and maintenance. Chromosome Res (2011) 0.97

Phosphorylation modulates clearance of alpha-synuclein inclusions in a yeast model of Parkinson's disease. PLoS Genet (2014) 0.96

Heterodimerization with different Jun proteins controls c-Fos intranuclear dynamics and distribution. J Biol Chem (2010) 0.96

Uncoupling diffusion and binding in FRAP experiments. Nat Methods (2009) 0.96

Vinculin controls talin engagement with the actomyosin machinery. Nat Commun (2015) 0.96

Dominant-negative mechanism of leukemogenic PAX5 fusions. Oncogene (2011) 0.95

Unwinding the differences of the mammalian PERIOD clock proteins from crystal structure to cellular function. Proc Natl Acad Sci U S A (2012) 0.95

In vivo biochemistry in bacterial cells using FRAP: insight into the translation cycle. Biophys J (2012) 0.94

FRAP analysis of membrane-associated proteins: lateral diffusion and membrane-cytoplasmic exchange. Biophys J (2010) 0.94

Quantitative kinetic study of the actin-bundling protein L-plastin and of its impact on actin turn-over. PLoS One (2010) 0.93

Dephosphorylation and caspase processing generate distinct nuclear pools of histone deacetylase 4. Mol Cell Biol (2007) 0.93

The dendritic cell cytoskeleton promotes T cell adhesion and activation by constraining ICAM-1 mobility. J Cell Biol (2015) 0.92

Multiple spatial and kinetic subpopulations of CaMKII in spines and dendrites as resolved by single-molecule tracking PALM. J Neurosci (2014) 0.92

Complex dynamics of transcription regulation. Biochim Biophys Acta (2012) 0.92

Fluorescence techniques to study lipid dynamics. Cold Spring Harb Perspect Biol (2011) 0.92

Myosin light chain mono- and di-phosphorylation differentially regulate adhesion and polarity in migrating cells. Biochem Biophys Res Commun (2010) 0.92

Analysis of membrane-localized binding kinetics with FRAP. Eur Biophys J (2008) 0.91

Diffusion and retention are major determinants of protein targeting to the inner nuclear membrane. J Cell Biol (2015) 0.91

Modeling cellular processes in 3D. Trends Cell Biol (2011) 0.90

Dissection of cell cycle-dependent dynamics of Dnmt1 by FRAP and diffusion-coupled modeling. Nucleic Acids Res (2013) 0.90

The histone methyltransferase SUV420H2 and Heterochromatin Proteins HP1 interact but show different dynamic behaviours. BMC Cell Biol (2009) 0.90

Continuous photobleaching in vesicles and living cells: a measure of diffusion and compartmentation. Biophys J (2006) 0.90

How to scale down postsynaptic strength. J Neurosci (2013) 0.89

Mechanism of the Ca²+-dependent interaction between S100A4 and tail fragments of nonmuscle myosin heavy chain IIA. J Mol Biol (2010) 0.89

Quantitative analysis of self-association and mobility of annexin A4 at the plasma membrane. Biophys J (2013) 0.88

Characterization of a naturally-occurring p27 mutation predisposing to multiple endocrine tumors. Mol Cancer (2010) 0.88

Selective roles for cholesterol and actin in compartmentalization of different proteins in the Golgi and plasma membrane of polarized cells. J Biol Chem (2008) 0.87

Distinct properties of human HMGN5 reveal a rapidly evolving but functionally conserved nucleosome binding protein. Mol Cell Biol (2011) 0.87

Diffusion and partitioning of fluorescent lipid probes in phospholipid monolayers. Biophys J (2009) 0.87

ATP-binding cassette B4, an auxin-efflux transporter, stably associates with the plasma membrane and shows distinctive intracellular trafficking from that of PIN-FORMED proteins. Plant Physiol (2012) 0.87

Recent developments in fluorescence correlation spectroscopy for diffusion measurements in planar lipid membranes. Int J Mol Sci (2010) 0.87

Ezrin mediates tethering of the gamma-aminobutyric acid transporter GAT1 to actin filaments via a C-terminal PDZ-interacting domain. Biophys J (2009) 0.86

Reversible aggregation of PABPN1 pre-inclusion structures. Nucleus (2011) 0.86

FRAP analysis: accounting for bleaching during image capture. PLoS One (2012) 0.86

Live cell imaging and systems biology. Wiley Interdiscip Rev Syst Biol Med (2010) 0.85

Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of β-arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors. Biochim Biophys Acta (2012) 0.84

Messages do diffuse faster than messengers: reconciling disparate estimates of the morphogen bicoid diffusion coefficient. PLoS Comput Biol (2014) 0.84

FERM domain phosphoinositide binding targets merlin to the membrane and is essential for its growth-suppressive function. Mol Cell Biol (2011) 0.84

Localization of GroEL determined by in vivo incorporation of a fluorescent amino acid. Bioorg Med Chem Lett (2011) 0.84

Light and life in Baltimore--and beyond. Biophys J (2015) 0.84

Dopamine Transporter Activity Is Modulated by α-Synuclein. J Biol Chem (2015) 0.84

Concentration dependence of lipopolymer self-diffusion in supported bilayer membranes. J R Soc Interface (2010) 0.83

Analysis of binding reactions by fluorescence recovery after photobleaching. Biophys J (2004) 5.12

Transcription dynamics. Mol Cell (2009) 4.15

Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks. J Cell Biol (2006) 3.63

Genome-wide protein-DNA binding dynamics suggest a molecular clutch for transcription factor function. Nature (2012) 2.85

Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes. Mol Cell Biol (2004) 2.71

SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. J Cell Biol (2002) 2.60

Dynamic behavior of transcription factors on a natural promoter in living cells. EMBO Rep (2002) 2.21

Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter. Science (2008) 2.08

Evidence for a common mode of transcription factor interaction with chromatin as revealed by improved quantitative fluorescence recovery after photobleaching. Biophys J (2008) 2.04

Three-dimensional cellular ultrastructure resolved by X-ray microscopy. Nat Methods (2010) 1.89

Analysis of binding at a single spatially localized cluster of binding sites by fluorescence recovery after photobleaching. Biophys J (2006) 1.78

FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know? Curr Opin Cell Biol (2010) 1.64

Quantitation of GFP-fusion proteins in single living cells. J Struct Biol (2002) 1.59

A benchmark for chromatin binding measurements in live cells. Nucleic Acids Res (2012) 1.56

Hsp90 inhibition transiently activates Src kinase and promotes Src-dependent Akt and Erk activation. Proc Natl Acad Sci U S A (2006) 1.55

Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription. Nucleic Acids Res (2004) 1.52

Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy. Biophys J (2009) 1.52

Regulation of TATA-binding protein dynamics in living yeast cells. Proc Natl Acad Sci U S A (2008) 1.38

Measuring chromatin interaction dynamics on the second time scale at single-copy genes. Science (2013) 1.35

The interaction of NSBP1/HMGN5 with nucleosomes in euchromatin counteracts linker histone-mediated chromatin compaction and modulates transcription. Mol Cell (2009) 1.34

Jab1/CSN5, a component of the COP9 signalosome, regulates transforming growth factor beta signaling by binding to Smad7 and promoting its degradation. Mol Cell Biol (2004) 1.30

Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis. EMBO J (2010) 1.29

A reaction-diffusion model to study RNA motion by quantitative fluorescence recovery after photobleaching. Biophys J (2007) 1.27

Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates. Biophys J (2010) 1.27

Potential roles for ubiquitin and the proteasome during ribosome biogenesis. Mol Cell Biol (2006) 1.25

Prion induction by the short-lived, stress-induced protein Lsb2 is regulated by ubiquitination and association with the actin cytoskeleton. Mol Cell (2011) 1.24

Generic features of tertiary chromatin structure as detected in natural chromosomes. Mol Cell Biol (2004) 1.19

Fluorescence recovery after photobleaching (FRAP) methods for visualizing protein dynamics in living mammalian cell nuclei. Methods Enzymol (2004) 1.17

TLP, a novel modulator of TGF-beta signaling, has opposite effects on Smad2- and Smad3-dependent signaling. EMBO J (2003) 1.16

iLAP: a workflow-driven software for experimental protocol development, data acquisition and analysis. BMC Bioinformatics (2009) 1.14

Partner-regulated interaction of IFN regulatory factor 8 with chromatin visualized in live macrophages. Proc Natl Acad Sci U S A (2005) 1.14

Dynamic interactions of a transcription factor with DNA are accelerated by a chromatin remodeller. EMBO Rep (2004) 1.12

Quantifying transcription factor kinetics: at work or at play? Crit Rev Biochem Mol Biol (2013) 1.06

A flow cytometric method to detect protein-protein interaction in living cells by directly visualizing donor fluorophore quenching during CFP-->YFP fluorescence resonance energy transfer (FRET). Cytometry A (2003) 1.03

Flow cytometric measurement of fluorescence (Förster) resonance energy transfer from cyan fluorescent protein to yellow fluorescent protein using single-laser excitation at 458 nm. Cytometry A (2003) 1.02

Intracellular delivery of acetyl-histone peptides inhibits native bromodomain-chromatin interactions and impairs mitotic progression. FEBS Lett (2008) 1.01

Minimizing the impact of photoswitching of fluorescent proteins on FRAP analysis. Biophys J (2012) 1.00

Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload. J Cell Biol (2011) 1.00

Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleaching. Curr Protoc Cytom (2006) 1.00

Requirement for the budding yeast polo kinase Cdc5 in proper microtubule growth and dynamics. Eukaryot Cell (2008) 0.96

Transcription factories. Front Genet (2012) 0.95

Towards an atlas of mammalian cell ultrastructure by cryo soft X-ray tomography. J Struct Biol (2011) 0.94

Assembly of the transcription machinery: ordered and stable, random and dynamic, or both? Chromosoma (2011) 0.93

Organization of chromatin and histone modifications at a transcription site. J Cell Biol (2007) 0.93

Not choosing nursing: work experience and career choice of high academic achieving school leavers. Nurse Educ Today (2010) 0.92

Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching. Methods Mol Biol (2012) 0.90

Dynamic effect of bortezomib on nuclear factor-kappaB activity and gene expression in tumor cells. Mol Pharmacol (2008) 0.89

Core histone hyperacetylation impacts cooperative behavior and high-affinity binding of histone H1 to chromatin. Biochemistry (2010) 0.89

Myosin II redistribution during rear retraction and the role of filament assembly and disassembly. Cell Biol Int (2002) 0.88

Convergence of chromatin binding estimates in live cells. Nat Methods (2013) 0.87

Live cell imaging and systems biology. Wiley Interdiscip Rev Syst Biol Med (2010) 0.85

Through thick and thin: the conundrum of chromatin fibre folding in vivo. EMBO Rep (2012) 0.84

CKAP2 ensures chromosomal stability by maintaining the integrity of microtubule nucleation sites. PLoS One (2013) 0.81

Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy. Methods Mol Biol (2012) 0.81

Co-expressed genes prepositioned in spatial neighborhoods stochastically associate with SC35 speckles and RNA polymerase II factories. Cell Mol Life Sci (2013) 0.81

Role of H1 phosphorylation in rapid GR exchange and function at the MMTV promoter. Histochem Cell Biol (2005) 0.80

The arylstibonic acid compound NSC13746 disrupts B-ZIP binding to DNA in living cells. Eur J Cell Biol (2010) 0.79

Monitoring dynamic binding of chromatin proteins in vivo by single-molecule tracking. Methods Mol Biol (2013) 0.79

Genome-wide haploinsufficiency screen reveals a novel role for γ-TuSC in spindle organization and genome stability. Mol Biol Cell (2013) 0.77