Published in Protein Expr Purif on July 25, 2005
The impact of intragenic CpG content on gene expression. Nucleic Acids Res (2010) 1.11
Recombinant protein secretion in Escherichia coli. Biotechnol Adv (2005) 2.23
Trends in dengue diagnosis. Rev Med Virol (2005) 1.32
Mouse embryonic stem cell expansion in a microcarrier-based stirred culture system. J Biotechnol (2007) 1.12
Phytosterols: applications and recovery methods. Bioresour Technol (2006) 1.02
High sensitivity detection of molecular recognition using magnetically labelled biomolecules and magnetoresistive sensors. Biosens Bioelectron (2003) 1.01
Nonviral gene delivery to mesenchymal stem cells using cationic liposomes for gene and cell therapy. J Biomed Biotechnol (2010) 0.95
An on-chip thin film photodetector for the quantification of DNA probes and targets in microarrays. Nucleic Acids Res (2004) 0.91
Electric-field assisted immobilization and hybridization of DNA oligomers on thin-film microchips. Nanotechnology (2005) 0.91
Suppression of lymphocyte blastogenesis to mitogens in cats experimentally infected with feline immunodeficiency virus. Vet Immunol Immunopathol (1990) 0.91
Towards a cost effective strategy for cutinase production by a recombinant Saccharomyces cerevisiae: strain physiological aspects. Appl Microbiol Biotechnol (2003) 0.90
Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications. Trends Biotechnol (2000) 0.89
Detection of DNA and proteins using amorphous silicon ion-sensitive thin-film field effect transistors. Biosens Bioelectron (2008) 0.88
Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks. Trends Biotechnol (1999) 0.87
Antigenic and biological characterisation of avian paramyxovirus type I isolates from pigeons--an international collaborative study. Avian Pathol (1985) 0.86
Translational features of human alpha 2b interferon production in Escherichia coli. Appl Environ Microbiol (2004) 0.86
Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B. Application of the solvophobic theory. J Chromatogr A (2002) 0.84
Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes. Biomicrofluidics (2011) 0.84
Cellular specificity and replication rate of Maedi Visna virus in vitro can be controlled by LTR sequences. Arch Virol (2004) 0.83
Production, purification and analysis of an experimental DNA vaccine against rabies. J Gene Med (2002) 0.82
Integrated detection of intrinsic fluorophores in live microbial cells using an array of thin film amorphous silicon photodetectors. Biosens Bioelectron (2012) 0.82
Immobilization and hybridization by single sub-millisecond electric field pulses, for pixel-addressed DNA microarrays. Biosens Bioelectron (2004) 0.81
Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography. Biotechnol Bioeng (2000) 0.81
Chromatography of plasmid DNA. J Chromatogr A (2005) 0.81
Improving activity and stability of cutinase towards the anionic detergent AOT by complete saturation mutagenesis. Protein Eng Des Sel (2008) 0.80
Acute physiological responses to different circuit training protocols. J Sports Med Phys Fitness (2008) 0.80
Conjugal transfer of recombinant plasmids into gellan gum-producing and non-producing variants of Pseudomonas elodea ATCC 31461. Lett Appl Microbiol (1991) 0.80
Phylogenetic analysis of five Portuguese strains of FIV. Arch Virol (2002) 0.79
Application of central composite design for DNA hybridization onto magnetic microparticles. Anal Biochem (2009) 0.79
Micro-analytical GO/HRP bioreactor for glucose determination and bioprocess monitoring. Biosens Bioelectron (2005) 0.79
Isolation of plasmid DNA from cell lysates by aqueous two-phase systems. Biotechnol Bioeng (2002) 0.79
Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography. J Chromatogr A (2003) 0.79
Medium and copy number effects on the secretion of human proinsulin in Escherichia coli using the universal stress promoters uspA and uspB. Appl Microbiol Biotechnol (2003) 0.79
Optimization of isopropanol and ammonium sulfate precipitation steps in the purification of plasmid DNA. Biotechnol Prog (2006) 0.78
Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography. Arch Biochem Biophys (2007) 0.78
Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention. Biomed Chromatogr (2009) 0.78
Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples. Anal Bioanal Chem (2008) 0.78
Evaluation of sperm DNA peroxidation in fertile and subfertile dogs. Reprod Domest Anim (2012) 0.78
Evaluation of bottlenecks in proinsulin secretion by Escherichia coli. J Biotechnol (2004) 0.78
Isolation of a biodegradable sterol-rich fraction from industrial wastes. Bioresour Technol (2002) 0.78
Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products. Anal Biochem (2003) 0.78
On the stability of plasmid DNA vectors during cell culture and purification. Mol Biotechnol (2007) 0.78
Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification. Biomed Chromatogr (2009) 0.77
Isolation of a beta-carotene over-producing soil bacterium, Sphingomonas sp. Biotechnol Lett (2004) 0.77
Single base mismatch detection by microsecond voltage pulses. Biosens Bioelectron (2005) 0.77
Troubleshooting in gene splicing by overlap extension: a step-wise method. Mol Biotechnol (1999) 0.77
Immobilization of mycobacterial cells onto silicone--assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol. Bioresour Technol (2007) 0.77
Integration of production and aqueous two-phase systems extraction of extracellular Fusarium solani pisi cutinase fusion proteins. J Biotechnol (2003) 0.77
Comparing the effect of immobilization methods on the activity of lipase biocatalysts in ester hydrolysis. Bioprocess Biosyst Eng (2007) 0.77
Optimization of gene delivery to HEK293T cells by microporation using a central composite design methodology. Biotechnol Lett (2010) 0.77
Fluorescence of the single tryptophan of cutinase: temperature and pH effect on protein conformation and dynamics. Photochem Photobiol (2003) 0.77
Biochemical and structural characterisation of cutinase mutants in the presence of the anionic surfactant AOT. Biochim Biophys Acta (2008) 0.77
Behaviour of Mycobacterium sp. NRRL B-3805 whole cells in aqueous, organic-aqueous and organic media studied by fluorescence microscopy. Appl Microbiol Biotechnol (2003) 0.76
Purification of plasmids for gene therapy and DNA vaccination. Biotechnol Annu Rev (2001) 0.76
Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus. Int J Pharm (2009) 0.76
Phylogenetic analysis of six isolates of beak and feather disease virus from African grey parrots in Portugal. Avian Dis (2010) 0.76
Evaluation of the effect of non-B DNA structures on plasmid integrity via accelerated stability studies. J Pharm Sci (2009) 0.76
Horseradish peroxidase immobilized through its carboxylic groups onto a polyacrylonitrile membrane: comparison of enzyme performances with inorganic beaded supports. Appl Biochem Biotechnol (2003) 0.76
Modelling of ex vivo expansion/maintenance of hematopoietic stem cells. Bioprocess Biosyst Eng (2003) 0.75
Enhancing the thermal stability of lipases through mutagenesis and immobilization on zeolites. Bioprocess Biosyst Eng (2008) 0.75
A quantitative ELISA for monitoring the secretion of ZZ-fusion proteins using SpA domain as immunodetection reporter system. Mol Biotechnol (2001) 0.75
Factors enhancing lycopene production by a new Mycobacterium aurum mutant. Biotechnol Lett (2004) 0.75
A quantitative method to evaluate mesenchymal stem cell lipofection using real-time PCR. Biotechnol Prog (2010) 0.75
Kinetic modelling of phenol co-oxidation using horseradish peroxidase. Bioprocess Biosyst Eng (2006) 0.75
Scanning electron microscopy investigations on bis(2-ethylhexyl)phthalate treated Mycobacterium cells. Microsc Res Tech (2006) 0.75
Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography. Anal Biochem (2007) 0.75
New seminal plasma removal method for freezing stallion semen. Theriogenology (2013) 0.75
The effect of the shape of single, sub-ms voltage pulses on the rates of surface immobilization and hybridization of DNA. Nanotechnology (2008) 0.75
Activity, conformation and dynamics of cutinase adsorbed on poly(methyl methacrylate) latex particles. J Biotechnol (2003) 0.75
Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography. Biomed Chromatogr (2007) 0.75
Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters. Biosens Bioelectron (2013) 0.75
Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification. Biotechnol Lett (2009) 0.75
Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography. J Mol Recognit (2010) 0.75
Prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography. J Chromatogr A (2007) 0.75
Anti-idiotypic antibodies to feline leukemia virus: an approach for retroviral immunization strategies. Viral Immunol (1991) 0.75
The role of probe-probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles. Anal Bioanal Chem (2009) 0.75
Reappearance of swine vesicular disease virus in Portugal. Vet Rec (2007) 0.75
A novel pathway to enzyme deactivation: the cutinase model. Biotechnol Bioeng (2003) 0.75
Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support. Anal Biochem (2005) 0.75
Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized sepharose CL-6B. Using and relating two different models for describing the effect of salt and temperature on retention. J Chromatogr A (2003) 0.75
Separation and analysis of plasmid denatured forms using hydrophobic interaction chromatography. Anal Biochem (1999) 0.75
Chemical synthesis and crystallization of the dipeptide AcPheIleNH(2) in TTAB/heptane/octanol reversed micelles. J Colloid Interface Sci (2006) 0.75
Pacheco's parrot disease in macaws of the Lisbon's Zoological Garden. Description of an outbreak, diagnosis and management, including vaccination. Dtsch Tierarztl Wochenschr (2007) 0.75
Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant Saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography. Biotechnol Lett (2008) 0.75