PubRank

S G Withers

Author PubWeight™ 145.43‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Glycosyltransferases: structures, functions, and mechanisms. Annu Rev Biochem 2008 6.06
2 Mechanisms of enzymatic glycoside hydrolysis. Curr Opin Struct Biol 1994 2.96
3 Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs. Nat Struct Biol 2001 2.79
4 X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family. Nat Struct Biol 1999 2.58
5 Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate. Nature 2001 2.51
6 Glycosidase mechanisms: anatomy of a finely tuned catalyst. Acc Chem Res 2000 2.49
7 Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases. J Biol Chem 1992 2.10
8 Glycosidase mechanisms. Curr Opin Chem Biol 2000 1.72
9 Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli. J Biol Chem 1992 1.68
10 Mechanism of Agrobacterium beta-glucosidase: kinetic studies. Biochemistry 1992 1.66
11 Crystallographic evidence for substrate-assisted catalysis in a bacterial beta-hexosaminidase. J Biol Chem 2000 1.62
12 Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase. Biochemistry 1998 1.59
13 The structure of human pancreatic alpha-amylase at 1.8 A resolution and comparisons with related enzymes. Protein Sci 1995 1.54
14 Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase. Nat Struct Biol 1996 1.49
15 Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate. Science 1991 1.44
16 The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of bacillus circulans xylanase. Biochemistry 1996 1.43
17 Identification of the acid/base catalyst in Agrobacterium faecalis beta-glucosidase by kinetic analysis of mutants. Biochemistry 1995 1.39
18 Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase. Biochemistry 1999 1.37
19 Mutagenesis of glycosidases. Annu Rev Biochem 1999 1.36
20 A structural view of the action of Escherichia coli (lacZ) beta-galactosidase. Biochemistry 2001 1.35
21 Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase. J Biol Chem 1993 1.33
22 Cloning, expression, characterization, and nucleophile identification of family 3, Aspergillus niger beta-glucosidase. J Biol Chem 2000 1.30
23 Hydrogen bonding and specificity. Fluorodeoxy sugars as probes of hydrogen bonding in the glycogen phosphorylase-glucose complex. Biochemistry 1986 1.28
24 Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase. J Mol Biol 2000 1.26
25 Inactivation of a beta-glucosidase through the accumulation of a stable 2-deoxy-2-fluoro-alpha-D-glucopyranosyl-enzyme intermediate: a detailed investigation. Biochemistry 1992 1.21
26 Crystal structure of the catalytic domain of the beta-1,4-glycanase cex from Cellulomonas fimi. Biochemistry 1994 1.20
27 The necessity of magnesium cation for acid assistance aglycone departure in catalysis by Escherichia coli (lacZ) beta-galactosidase. Biochem J 1978 1.20
28 The acid/base catalyst in the exoglucanase/xylanase from Cellulomonas fimi is glutamic acid 127: evidence from detailed kinetic studies of mutants. Biochemistry 1994 1.20
29 The beta-galactosidase-catalysed hydrolyses of beta-d-galactopyranosyl pyridium salts. Rate-limiting generation of an enzyme-bound galactopyranosyl cation in a process dependent only on aglycone acidity. Biochem J 1974 1.18
30 Glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from Cellulomonas fimi. J Biol Chem 1991 1.18
31 Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry. Biochemistry 1994 1.17
32 Unequivocal identification of Asp-214 as the catalytic nucleophile of Saccharomyces cerevisiae alpha-glucosidase using 5-fluoro glycosyl fluorides. J Biol Chem 1996 1.17
33 Comparison of the binding of glucose and glucose 1-phosphate derivatives to T-state glycogen phosphorylase b. Biochemistry 1990 1.16
34 The cellulose-binding domains from Cellulomonas fimi beta-1, 4-glucanase CenC bind nitroxide spin-labeled cellooligosaccharides in multiple orientations. J Mol Biol 1999 1.16
35 Mechanism of Agrobacterium beta-glucosidase: kinetic analysis of the role of noncovalent enzyme/substrate interactions. Biochemistry 1995 1.15
36 Mechanisms of cellulases and xylanases: a detailed kinetic study of the exo-beta-1,4-glycanase from Cellulomonas fimi. Biochemistry 1994 1.11
37 Glycosyl fluorides in enzymatic reactions. Carbohydr Res 2000 1.10
38 Direct 1H n.m.r. determination of the stereochemical course of hydrolyses catalysed by glucanase components of the cellulase complex. Biochem Biophys Res Commun 1986 1.09
39 Dissection of nucleophilic and acid-base catalysis in glycosidases. Curr Opin Chem Biol 2001 1.09
40 Dissecting the electrostatic interactions and pH-dependent activity of a family 11 glycosidase. Biochemistry 2001 1.08
41 Catalytic site of glycogen phosphorylase: structural changes during activation and mechanistic implications. Biochemistry 1982 1.08
42 Binding energy and catalysis. Fluorinated and deoxygenated glycosides as mechanistic probes of Escherichia coli (lacZ) beta-galactosidase. Biochem J 1992 1.06
43 Fluorinated and deoxygenated substrates as probes of transition-state structure in glycogen phosphorylase. Biochemistry 1989 1.05
44 Reassessment of the catalytic mechanism of glycogen debranching enzyme. Biochemistry 1991 1.05
45 Identification of the active site nucleophile in jack bean alpha-mannosidase using 5-fluoro-beta-L-gulosyl fluoride. J Biol Chem 1998 1.05
46 Site-directed mutation of the putative catalytic residues of endoglucanase CenA from Cellulomonas fimi. Biochemistry 1995 1.04
47 Subsite mapping of the human pancreatic alpha-amylase active site through structural, kinetic, and mutagenesis techniques. Biochemistry 2000 1.03
48 Region-directed mutagenesis of residues surrounding the active site nucleophile in beta-glucosidase from Agrobacterium faecalis. J Biol Chem 1992 1.03
49 Identification of Glu340 as the active-site nucleophile in human glucocerebrosidase by use of electrospray tandem mass spectrometry. J Biol Chem 1994 1.02
50 Mechanism-based inhibition of yeast alpha-glucosidase and human pancreatic alpha-amylase by a new class of inhibitors. 2-Deoxy-2,2-difluoro-alpha-glycosides. J Biol Chem 1995 1.01
51 Characterization of the Glu and Asp residues in the active site of human beta-hexosaminidase B. Biochemistry 2001 1.00
52 Mechanism of action and identification of Asp242 as the catalytic nucleophile of Vibrio furnisii N-acetyl-beta-D-glucosaminidase using 2-acetamido-2-deoxy-5-fluoro-alpha-L-idopyranosyl fluoride. Biochemistry 2000 0.99
53 The purification and characterization of a beta-glucosidase from Alcaligenes faecalis. Biochem Cell Biol 1986 0.99
54 Reassessment of acarbose as a transition state analogue inhibitor of cyclodextrin glycosyltransferase. Biochemistry 1998 0.99
55 Identification of Glu-540 as the catalytic nucleophile of human beta-glucuronidase using electrospray mass spectrometry. J Biol Chem 1998 0.99
56 Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. J Biol Chem 1997 0.98
57 Substrate specificity of endoglucanase A from Cellulomonas fimi: fundamental differences between endoglucanases and exoglucanases from family 6. Biochem J 1996 0.98
58 Mechanistic consequences of mutation of active site carboxylates in a retaining beta-1,4-glycanase from Cellulomonas fimi. Biochemistry 1996 0.98
59 Identification of Glu-268 as the catalytic nucleophile of human lysosomal beta-galactosidase precursor by mass spectrometry. J Biol Chem 1997 0.98
60 Active form of pyridoxal phosphate in glycogen phosphorylase. Phosphorus-31 nuclear magentic resonance investigation. Biochemistry 1981 0.97
61 Evidence for direct phosphate-phosphate interaction between pyridoxal phosphate and substrate in the glycogen phosphorylase catalytic mechanism. J Biol Chem 1981 0.97
62 Biochemical and structural assessment of the 1-N-azasugar GalNAc-isofagomine as a potent family 20 beta-N-acetylhexosaminidase inhibitor. J Biol Chem 2001 0.96
63 Differential mechanism-based labeling and unequivocal activity assignment of the two active sites of intestinal lactase/phlorizin hydrolase. Eur J Biochem 2000 0.96
64 Glycosyl fluorides can function as substrates for nucleotide phosphosugar-dependent glycosyltransferases. J Biol Chem 1999 0.95
65 The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 A resolution. Biochemistry 1999 0.95
66 Catalytic site of glycogen phosphorylase: structure of the T state and specificity for alpha-D-glucose. Biochemistry 1982 0.94
67 Pre-steady state kinetic analysis of an enzymatic reaction monitored by time-resolved electrospray ionization mass spectrometry. Biochemistry 1998 0.94
68 A solid-state 31P-NMR investigation of the allosteric transition in glycogen phosphorylase b. Biophys J 1993 0.94
69 Ternary complex crystal structures of glycogen phosphorylase with the transition state analogue nojirimycin tetrazole and phosphate in the T and R states. Biochemistry 1996 0.93
70 Suicide inactivation of human prostatic acid phosphatase and a phosphotyrosine phosphatase. Biochem Biophys Res Commun 1994 0.93
71 Identification of Glu-330 as the catalytic nucleophile of Candida albicans exo-beta-(1,3)-glucanase. J Biol Chem 1997 0.93
72 Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants. Nat Struct Biol 1998 0.92
73 Identification of Asp197 as the catalytic nucleophile in the family 38 alpha-mannosidase from bovine kidney lysosomes. FEBS Lett 2000 0.91
74 Exploring the cellulose/xylan specificity of the beta-1,4-glycanase cex from Cellulomonas fimi through crystallography and mutation. Biochemistry 1998 0.91
75 The E358S mutant of Agrobacterium sp. beta-glucosidase is a greatly improved glycosynthase. FEBS Lett 2000 0.91
76 Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity. Biochemistry 1996 0.91
77 Kinetic mechanism and characterization of human beta-galactosidase precursor secreted by permanently transfected Chinese hamster ovary cells. Biochem J 1994 0.90
78 Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate. Biochem J 1998 0.90
79 Mechanisms of cellulases and xylanases. Biochem Soc Trans 1998 0.90
80 Powerful probes for glycosidases: novel, fluorescently tagged glycosidase inhibitors. Bioorg Med Chem Lett 2001 0.90
81 The synthesis, testing and use of 5-fluoro-alpha-D-galactosyl fluoride to trap an intermediate on green coffee bean alpha-galactosidase and identify the catalytic nucleophile. Carbohydr Res 2000 0.90
82 Stereochemistry and kinetics of the hydration of 2-acetamido-D-glucal by beta-N-acetylhexosaminidases. Biochemistry 1994 0.88
83 Identification of Asp-130 as the catalytic nucleophile in the main alpha-galactosidase from Phanerochaete chrysosporium, a family 27 glycosyl hydrolase. Biochemistry 2000 0.87
84 Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris. Protein Sci 1999 0.87
85 Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase. Biochemistry 1997 0.87
86 Identical structural changes induced in glycogen phosphorylase by two nonexclusive allosteric inhibitors. Biochemistry 1979 0.86
87 Covalently activated glycogen phosphorylase: a phosphorus-31 nuclear magnetic resonance and ultracentrifugation analysis. Biochemistry 1982 0.86
88 Rapid screening of the aglycone specificity of glycosidases: applications to enzymatic synthesis of oligosaccharides. Chem Biol 2001 0.86
89 Pyridoxal phosphate is not the acid catalyst in the glycogen phosphorylase catalytic mechanism. Biochem Biophys Res Commun 1982 0.86
90 Identification of the catalytic nucleophile of endoglucanase I from Fusarium oxysporum by mass spectrometry. Biochemistry 1997 0.85
91 Identification of the catalytic nucleophile of the Family 31 alpha-glucosidase from Aspergillus niger via trapping of a 5-fluoroglycosyl-enzyme intermediate. Biochem J 2001 0.84
92 Simultaneous high-performance liquid chromatographic determination of both the cleavage pattern and the stereochemical outcome of the hydrolysis reactions catalyzed by various glycosidases. Anal Biochem 1993 0.84
93 31P NMR relaxation studies of the activation of the coenzyme phosphate of glycogen phosphorylase. The role of motion of the bound phosphate. Biophys J 1985 0.84
94 Trapping and characterization of the reaction intermediate in cyclodextrin glycosyltransferase by use of activated substrates and a mutant enzyme. Biochemistry 1997 0.84
95 Syntheses and testing of substrates and mechanism-based inactivators for xylanases. Carbohydr Res 1995 0.84
96 Syntheses of 2-deoxy-2-fluoro mono- and oligo-saccharide glycosides from glycals and evaluation as glycosidase inhibitors. Carbohydr Res 1993 0.84
97 Multiple phosphate positions in the catalytic site of glycogen phosphorylase: structure of the pyridoxal-5'-pyrophosphate coenzyme-substrate analog. Protein Sci 1992 0.83
98 Identification of glu-277 as the catalytic nucleophile of Thermoanaerobacterium saccharolyticum beta-xylosidase using electrospray MS. Biochem J 1998 0.83
99 Conduritol aziridine: a new mechanism-based glucosidase inactivator. Biochem Biophys Res Commun 1989 0.81
100 Identification of Glu-519 as the catalytic nucleophile in beta-mannosidase 2A from Cellulomonas fimi. Biochem J 2000 0.81
101 Identification of derivatized peptides without radiolabels: tandem mass spectrometric localization of the tagged active-site nucleophiles of two cellulases and a beta-glucosidase. Anal Biochem 1995 0.81
102 The identification of the catalytic nucleophiles of two beta-galactosidases from glycoside hydrolase family 35. Carbohydr Res 2001 0.81
103 The three-dimensional structure of acarbose bound to glycogen phosphorylase. J Biol Chem 1987 0.81
104 N-bromoacetyl-glycopyranosylamines as affinity labels for a beta-glucosidase and a cellulase. Carbohydr Res 1993 0.81
105 Binding energy and catalysis: deoxyfluoro sugars as probes of hydrogen bonding in phosphoglucomutase. Biochemistry 1992 0.81
106 Identification of Glu-120 as the catalytic nucleophile in Streptomyces lividans endoglucanase celB. Biochem J 1998 0.81
107 Interaction of the lacZ beta-galactosidase of Escherichia coli with some beta-D-galactopyranoside competitive inhibitors. Biochem J 1979 0.80
108 Nucleotide activation of glycogen phosphorylase b occurs only when the nucleotide phosphate is in a dianionic form. Biochem Biophys Res Commun 1980 0.80
109 Isolation, crystallization and preliminary diffraction analyses of human pancreatic alpha-amylase. J Mol Biol 1993 0.80
110 Substrate-induced inactivation of a crippled beta-glucosidase mutant: identification of the labeled amino acid and mutagenic analysis of its role. Biochemistry 1995 0.80
111 Dependence upon pH of steady-state parameters for the beta-galactosidase-catalysed hydrolyses of beta-D-galactopyranosyl derivatives of different chemical types. Eur J Biochem 1978 0.80
112 In vivo inhibition of beta-glucosidase and beta-mannosidase activity in rats by 2-deoxy-2-fluoro-beta-glycosyl fluorides and recovery of activity in vivo and in vitro. Biochem J 1994 0.79
113 Enzymatic synthesis of carbon-fluorine bonds. J Am Chem Soc 2001 0.79
114 Directed evolution of new glycosynthases from Agrobacterium beta-glucosidase: a general screen to detect enzymes for oligosaccharide synthesis. Chem Biol 2001 0.79
115 Detailed structural analysis of glycosidase/inhibitor interactions: complexes of Cex from Cellulomonas fimi with xylobiose-derived aza-sugars. Biochemistry 2000 0.79
116 An assay for the transferase activity of glycogen debranching enzyme. Carbohydr Res 1995 0.79
117 Synthesis and kinetic evaluation of 4-deoxymaltopentaose and 4-deoxymaltohexaose as inhibitors of muscle and potato alpha-glucan phosphorylases. Biochem J 1999 0.79
118 Identification of Asp 549 as the catalytic nucleophile of glycogen-debranching enzyme via trapping of the glycosyl-enzyme intermediate. Biochemistry 1996 0.79
119 Pyridoxal(5')diphospho(1)-alpha-D-glucose. A potent R-state inhibitor of glycogen phosphorylase. J Biol Chem 1985 0.79
120 Human lysosomal and jack bean alpha-mannosidases are retaining glycosidases. Biochem Biophys Res Commun 1997 0.79
121 Novel, lipophilic derivatives of 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) are powerful beta-glucosidase inhibitors. Bioorg Med Chem Lett 2001 0.78
122 A mass spectrometry-based approach for probing enzyme active sites: identification of Glu 127 in Cellulomonas fimi exoglycanase as the residue modified by N-bromoacetyl cellobiosylamine. Anal Biochem 1996 0.78
123 Phosphonate and alpha-fluorophosphonate analogue probes of the ionization state of pyridoxal 5'-phosphate (PLP) in glycogen phosphorylase. Biochemistry 1996 0.78
124 Identification of active site residues in glycosidases by use of tandem mass spectrometry. Methods Mol Biol 2000 0.77
125 Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate. Protein Sci 2000 0.77
126 Crystallization and preliminary X-ray diffraction analysis of the catalytic domain of Cex, an exo-beta-1,4-glucanase and beta-1,4-xylanase from the bacterium Cellulomonas fimi. J Mol Biol 1992 0.77
127 A simple kinetic test to distinguish exo-glucosidase and beta-glucosidase activities. Biochem Biophys Res Commun 1985 0.77
128 Substituted glycals as probes of glycosidase mechanisms. Bioorg Med Chem 1996 0.77
129 Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitors. Eur J Biochem 1995 0.76
130 N-Acetyl glycals are tight-binding and environmentally insensitive inhibitors of hexosaminidases. Chem Commun (Camb) 2016 0.76
131 Mechanistic consequences of replacing the active-site nucleophile Glu-358 in Agrobacterium sp. beta-glucosidase with a cysteine residue. Biochem J 1998 0.75
132 19F NMR investigations of the catalytic mechanism of phosphoglucomutase using fluorinated substrates and inhibitors. Biochemistry 1992 0.75
133 Salicortin: a repeat-attack new-mechanism-based Agrobacterium faecalis beta-glucosidase inhibitor. Biochem J 1998 0.75
134 Probing the ionization state of substrate alpha-D-glucopyranosyl phosphate bound to glycogen phosphorylase b. Biochem J 1995 0.75
135 A systematic approach to isomorphous replacement using a series of simple charged mercurials. Biochem Biophys Res Commun 1979 0.75
136 Phosphodiesters serve as potentially tunable aglycones for fluoro sugar inactivators of retaining β-glycosidases. Org Biomol Chem 2014 0.75
137 Labeling and identification of the postulated acid/base catalyst in the alpha-glucosidase from Saccharomyces cerevisiae using a novel bromoketone C-glycoside. Biochemistry 1998 0.75
138 The catalytic mechanism of phosphorylase; novel role of the coenzyme phosphate. Prog Clin Biol Res 1984 0.75
139 Assignment of selectively 13C-labeled cellopentaose synthesized using an engineered glycosynthase. J Biomol NMR 2001 0.75
140 Understanding viral neuraminidase inhibition by substituted difluorosialic acids. Chem Commun (Camb) 2015 0.75
141 Synthesis and evaluation of chitosan-based, affinity-chromatography materials. Carbohydr Res 1988 0.75