Published in Biotechnol Bioeng on June 05, 2006
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb Cell Fact (2008) 1.89
Microbial factories for recombinant pharmaceuticals. Microb Cell Fact (2009) 1.59
Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays. BMC Genomics (2008) 1.36
A multi-level study of recombinant Pichia pastoris in different oxygen conditions. BMC Syst Biol (2010) 1.28
The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins. Microb Cell Fact (2010) 1.23
Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris. Microb Cell Fact (2006) 1.18
Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts. Appl Environ Microbiol (2007) 1.12
Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction. Microb Cell Fact (2007) 1.12
Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels. Microb Cell Fact (2009) 1.11
Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris. Microb Cell Fact (2013) 1.09
Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions. BMC Genomics (2007) 1.09
Expression of recombinant antibodies. Front Immunol (2013) 1.06
GAP promoter library for fine-tuning of gene expression in Pichia pastoris. Appl Environ Microbiol (2011) 1.03
Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity. PLoS One (2012) 1.03
The response to unfolded protein is involved in osmotolerance of Pichia pastoris. BMC Genomics (2010) 1.02
Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris. Microb Cell Fact (2008) 0.99
Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris. Microb Cell Fact (2011) 0.95
High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies. J Ind Microbiol Biotechnol (2010) 0.93
Enhanced production of recombinant secretory proteins in Pichia pastoris by optimizing Kex2 P1' site. PLoS One (2013) 0.90
Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta. Appl Environ Microbiol (2007) 0.89
Engineering eukaryotic protein factories. Biotechnol Lett (2007) 0.88
Advances in recombinant antibody manufacturing. Appl Microbiol Biotechnol (2016) 0.85
Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter. J Ind Microbiol Biotechnol (2007) 0.85
Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum. Microb Cell Fact (2014) 0.85
Constitutive expression of human angiostatin in Pichia pastoris by high-density cell culture. J Ind Microbiol Biotechnol (2006) 0.84
Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures. Microb Cell Fact (2014) 0.83
Pichia pastoris Aft1--a novel transcription factor, enhancing recombinant protein secretion. Microb Cell Fact (2014) 0.81
An updated view on horseradish peroxidases: recombinant production and biotechnological applications. Appl Microbiol Biotechnol (2015) 0.81
Biochemical and biophysical characterization of humanized IgG1 produced in Pichia pastoris. MAbs (2011) 0.81
Production in yeast of pseudotype virus-like particles harboring functionally active antibody fragments neutralizing the cytolytic activity of vaginolysin. Microb Cell Fact (2011) 0.81
Two-color selection for amplified co-production of proteins in mammalian cells. Protein Expr Purif (2007) 0.79
Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism. PLoS One (2016) 0.78
Growth-rate dependency of de novo resveratrol production in chemostat cultures of an engineered Saccharomyces cerevisiae strain. Microb Cell Fact (2015) 0.77
A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix. Sci Rep (2016) 0.75
Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris. AMB Express (2017) 0.75
Hansenula polymorpha Hac1p Is Critical to Protein N-Glycosylation Activity Modulation, as Revealed by Functional and Transcriptomic Analyses. Appl Environ Microbiol (2015) 0.75
Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris. Microb Cell Fact (2017) 0.75
Presep: predicting the propensity of a protein being secreted into the supernatant when expressed in Pichia pastoris. PLoS One (2013) 0.75
The bud tip is the cellular hot spot of protein secretion in yeasts. Appl Microbiol Biotechnol (2016) 0.75
Production of human pro-relaxin H2 in the yeast Pichia pastoris. BMC Biotechnol (2017) 0.75
Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris. BMC Biotechnol (2015) 0.75
Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies. Science (2005) 9.04
Comprehensive cross-clade neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodies. J Virol (2004) 8.99
Antibody domain exchange is an immunological solution to carbohydrate cluster recognition. Science (2003) 6.68
Broadly neutralizing anti-HIV antibody 4E10 recognizes a helical conformation of a highly conserved fusion-associated motif in gp41. Immunity (2005) 4.26
Microbial production of organic acids: expanding the markets. Trends Biotechnol (2008) 2.88
Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides. Proteomics (2008) 2.26
The long third complementarity-determining region of the heavy chain is important in the activity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2F5. J Virol (2004) 2.02
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb Cell Fact (2008) 1.89
Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol Bioeng (2004) 1.64
Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris. Microb Cell Fact (2009) 1.63
Binding of the 2F5 monoclonal antibody to native and fusion-intermediate forms of human immunodeficiency virus type 1 gp41: implications for fusion-inducing conformational changes. J Virol (2004) 1.58
The effect of temperature on the proteome of recombinant Pichia pastoris. J Proteome Res (2009) 1.53
In planta protein sialylation through overexpression of the respective mammalian pathway. J Biol Chem (2010) 1.52
Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous beta1,4-galactosylated N-glycan profile. J Biol Chem (2009) 1.49
Characterization of human class-switched polymeric (immunoglobulin M [IgM] and IgA) anti-human immunodeficiency virus type 1 antibodies 2F5 and 2G12. J Virol (2003) 1.45
Reassessment of autoreactivity of the broadly neutralizing HIV antibodies 4E10 and 2F5 and retrospective analysis of clinical safety data. AIDS (2007) 1.44
Inhibition of human immunodeficiency virus type 1 entry in cells expressing gp41-derived peptides. J Virol (2004) 1.43
Recombinant protein production in yeasts. Mol Biotechnol (2005) 1.38
Fc-glycosylation influences Fcγ receptor binding and cell-mediated anti-HIV activity of monoclonal antibody 2G12. J Immunol (2010) 1.36
Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays. BMC Genomics (2008) 1.36
Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern. Plant Biotechnol J (2007) 1.34
Membrane association and epitope recognition by HIV-1 neutralizing anti-gp41 2F5 and 4E10 antibodies. AIDS Res Hum Retroviruses (2006) 1.30
Adaptive laboratory evolution -- principles and applications for biotechnology. Microb Cell Fact (2013) 1.29
A multi-level study of recombinant Pichia pastoris in different oxygen conditions. BMC Syst Biol (2010) 1.28
Inhibition of in vivo HIV infection in humanized mice by gene therapy of human hematopoietic stem cells with a lentiviral vector encoding a broadly neutralizing anti-HIV antibody. J Virol (2010) 1.24
A close look at human IgG sialylation and subclass distribution after lectin fractionation. Proteomics (2009) 1.24
How to join a wave: decision-making processes in shimmering behavior of Giant honeybees (Apis dorsata). PLoS One (2012) 1.20
Pichia pastoris: protein production host and model organism for biomedical research. Future Microbiol (2013) 1.19
Hypoxic fed-batch cultivation of Pichia pastoris increases specific and volumetric productivity of recombinant proteins. Biotechnol Bioeng (2008) 1.19
Divergent genetic control of protein solubility and conformational quality in Escherichia coli. J Mol Biol (2007) 1.18
Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris. Microb Cell Fact (2006) 1.18
Antiviral activity of the neutralizing antibodies 2F5 and 2G12 in asymptomatic HIV-1-infected humans: a phase I evaluation. AIDS (2002) 1.17
Stereoscopic motion analysis in densely packed clusters: 3D analysis of the shimmering behaviour in Giant honey bees. Front Zool (2011) 1.17
New trends in bioinformatics: from genome sequence to personalized medicine. Exp Gerontol (2003) 1.15
Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production. J Biotechnol (2010) 1.13
Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans. Plant Biotechnol J (2007) 1.13
Specific phospholipid recognition by human immunodeficiency virus type-1 neutralizing anti-gp41 2F5 antibody. FEBS Lett (2006) 1.13
Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation. Microb Cell Fact (2004) 1.13
Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts. Appl Environ Microbiol (2007) 1.12
Electron microscopic and immunochemical analysis of the broadly neutralizing HIV-1-specific, anti-carbohydrate antibody, 2G12. Mol Immunol (2004) 1.11
Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels. Microb Cell Fact (2009) 1.11
Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris. Microb Cell Fact (2013) 1.09
Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus. FEMS Yeast Res (2009) 1.09
Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions. BMC Genomics (2007) 1.09
HIV-1 mutants escaping neutralization by the human antibodies 2F5, 2G12, and 4E10: in vitro experiments versus clinical studies. AIDS (2005) 1.08
The broadly neutralizing anti-human immunodeficiency virus type 1 4E10 monoclonal antibody is better adapted to membrane-bound epitope recognition and blocking than 2F5. J Virol (2008) 1.08
Anti-neuroblastoma effect of ch14.18 antibody produced in CHO cells is mediated by NK-cells in mice. Mol Immunol (2005) 1.08
A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris. PLoS One (2011) 1.08
Intracellular pH distribution in Saccharomyces cerevisiae cell populations, analyzed by flow cytometry. Appl Environ Microbiol (2005) 1.08
Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors. Biotechnol Bioeng (2006) 1.07
Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production. Biotechnol J (2010) 1.07
Biochemistry of microbial itaconic acid production. Front Microbiol (2013) 1.06
Improvement of lactic acid production in Saccharomyces cerevisiae by cell sorting for high intracellular pH. Appl Environ Microbiol (2006) 1.06
Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics. Biotechnol Adv (2012) 1.05
Biosynthesis of vitamin C by yeast leads to increased stress resistance. PLoS One (2007) 1.03
Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line. Biotechnol Prog (2005) 1.03
Production of recombinant proteins and metabolites in yeasts: when are these systems better than bacterial production systems? Appl Microbiol Biotechnol (2010) 1.02
The response to unfolded protein is involved in osmotolerance of Pichia pastoris. BMC Genomics (2010) 1.02
The secretory pathway: exploring yeast diversity. FEMS Microbiol Rev (2013) 1.01
Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering. J Biotechnol (2011) 1.01
Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris. Microb Cell Fact (2008) 0.99
In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response. Biotechnol J (2014) 0.99
Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5. Mol Immunol (2007) 0.98
Six novel constitutive promoters for metabolic engineering of Aspergillus niger. Appl Microbiol Biotechnol (2012) 0.98
Java editor for biological pathways. Bioinformatics (2003) 0.98
Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures. FASEB J (2007) 0.97
The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis. BMC Genomics (2011) 0.97
Ch14.18 antibody produced in CHO cells in relapsed or refractory Stage 4 neuroblastoma patients: a SIOPEN Phase 1 study. MAbs (2013) 0.97
Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast. Free Radic Biol Med (2012) 0.96
Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis. Plant Physiol (2011) 0.96
Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts. FEMS Microbiol Lett (2010) 0.96
Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export. Microb Cell Fact (2006) 0.95
Targeting enzymes to the right compartment: metabolic engineering for itaconic acid production by Aspergillus niger. Metab Eng (2013) 0.95
Partial humanization and characterization of an anti-idiotypic antibody against monoclonal antibody 2F5, a potential HIV vaccine? AIDS Res Hum Retroviruses (2007) 0.95
Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris. Microb Cell Fact (2011) 0.95
One-step triplex high-resolution melting analysis for rapid identification and simultaneous subtyping of frequently isolated Salmonella serovars. Appl Environ Microbiol (2012) 0.94
Production of monoclonal antibodies with a controlled N-glycosylation pattern in seeds of Arabidopsis thaliana. Plant Biotechnol J (2011) 0.94
Yeast systems biotechnology for the production of heterologous proteins. FEMS Yeast Res (2009) 0.94
Genome sequence of the ruminal bacterium Megasphaera elsdenii. J Bacteriol (2011) 0.94
Engineering of protein folding and secretion-strategies to overcome bottlenecks for efficient production of recombinant proteins. Antioxid Redox Signal (2014) 0.93
Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells. Biotechnol Bioeng (2006) 0.93
Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth. J Biotechnol (2011) 0.93
Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J Biotechnol (2003) 0.93
A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits. Glycobiology (2008) 0.93
Recognition and blocking of HIV-1 gp41 pre-transmembrane sequence by monoclonal 4E10 antibody in a Raft-like membrane environment. J Biol Chem (2006) 0.92