Virus-induced gene silencing as a tool for functional analyses in the emerging model plant Aquilegia (columbine, Ranunculaceae).

PubWeight™: 1.16‹?› | Rank: Top 10%

🔗 View Article (PMC 1855323)

Published in Plant Methods on April 12, 2007

Authors

Billie Gould1, Elena M Kramer

Author Affiliations

1: Department of Organismic and Evolutionary Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA. bgould@oeb.harvard.edu

Articles citing this

A ligation-independent cloning tobacco rattle virus vector for high-throughput virus-induced gene silencing identifies roles for NbMADS4-1 and -2 in floral development. Plant Physiol (2007) 1.27

A virus-induced gene silencing approach to understanding alkaloid metabolism in Catharanthus roseus. Phytochemistry (2011) 1.11

Reverse genetics of floral scent: application of tobacco rattle virus-based gene silencing in Petunia. Plant Physiol (2007) 1.11

Aquilegia as a model system for the evolution and ecology of petals. Philos Trans R Soc Lond B Biol Sci (2010) 1.05

Poppy APETALA1/FRUITFULL orthologs control flowering time, branching, perianth identity, and fruit development. Plant Physiol (2012) 1.01

Virus-induced gene silencing as a tool for comparative functional studies in Thalictrum. PLoS One (2010) 1.00

Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus. Plant Methods (2008) 0.94

TRV-GFP: a modified Tobacco rattle virus vector for efficient and visualizable analysis of gene function. J Exp Bot (2013) 0.94

Loss of deeply conserved C-class floral homeotic gene function and C- and E-class protein interaction in a double-flowered ranunculid mutant. Proc Natl Acad Sci U S A (2012) 0.93

Adaptive radiations: from field to genomic studies. Proc Natl Acad Sci U S A (2009) 0.92

Virus-induced gene silencing, a post transcriptional gene silencing method. Int J Plant Genomics (2009) 0.90

Contribution of host intracellular transport machineries to intercellular movement of turnip mosaic virus. PLoS Pathog (2013) 0.87

Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis. PLoS One (2010) 0.86

Virus-induced gene silencing (VIGS) in Cysticapnos vesicaria, a zygomorphic-flowered Papaveraceae (Ranunculales, basal eudicots). Ann Bot (2012) 0.82

Evolving Ideas on the Origin and Evolution of Flowers: New Perspectives in the Genomic Era. Genetics (2016) 0.82

Environmental and molecular analysis of the floral transition in the lower eudicot Aquilegia formosa. Evodevo (2011) 0.81

Retracted Virus-induced gene silencing for functional analysis of selected genes. Plant Cell Rep (2007) 0.81

The evolution of flower development: current understanding and future challenges. Ann Bot (2011) 0.80

Characteristic of the pepper CaRGA2 gene in defense responses against Phytophthora capsici Leonian. Int J Mol Sci (2013) 0.80

Current trends and future directions in flower development research. Ann Bot (2014) 0.80

Basic helix-loop-helix transcription factor BcbHLHpol functions as a positive regulator of pollen development in non-heading Chinese cabbage. Funct Integr Genomics (2014) 0.79

An Optimized Protocol to Increase Virus-Induced Gene Silencing Efficiency and Minimize Viral Symptoms in Petunia. Plant Mol Biol Report (2014) 0.78

Efficient gene silencing mediated by tobacco rattle virus in an emerging model plant physalis. PLoS One (2014) 0.78

A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing. J Exp Bot (2016) 0.76

Molecular basis for three-dimensional elaboration of the Aquilegia petal spur. Proc Biol Sci (2015) 0.76

Virus-induced gene silencing for comparative functional studies in Gladiolus hybridus. Plant Cell Rep (2013) 0.76

Reverse Genetics and High Throughput Sequencing Methodologies for Plant Functional Genomics. Curr Genomics (2016) 0.75

Genome-Wide Identification of BAHD Acyltransferases and In vivo Characterization of HQT-like Enzymes Involved in Caffeoylquinic Acid Synthesis in Globe Artichoke. Front Plant Sci (2016) 0.75

Conserved roles for Polycomb Repressive Complex 2 in the regulation of lateral organ development in Aquilegia x coerulea 'Origami'. BMC Plant Biol (2013) 0.75

Articles cited by this

Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J (1998) 97.93

Genes directing flower development in Arabidopsis. Plant Cell (1989) 11.56

Genetics and Biochemistry of Anthocyanin Biosynthesis. Plant Cell (1995) 5.06

Technical Advance. Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J (2001) 4.46

Function and regulation of the Arabidopsis floral homeotic gene PISTILLATA. Genes Dev (1994) 4.36

GLOBOSA: a homeotic gene which interacts with DEFICIENS in the control of Antirrhinum floral organogenesis. EMBO J (1992) 3.61

Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J (2004) 2.88

Molecular evolution of genes controlling petal and stamen development: duplication and divergence within the APETALA3 and PISTILLATA MADS-box gene lineages. Genetics (1998) 2.76

Development of a virus-induced gene-silencing system for hexaploid wheat and its use in functional analysis of the Lr21-mediated leaf rust resistance pathway. Plant Physiol (2005) 2.02

Columbines: a geographically widespread species flock. Proc Natl Acad Sci U S A (1994) 1.72

VIGS vectors for gene silencing: many targets, many tools. Annu Rev Plant Biol (2004) 1.62

Virus-induced gene silencing is an effective tool for assaying gene function in the basal eudicot species Papaver somniferum (opium poppy). Plant J (2005) 1.58

The duplicated B-class heterodimer model: whorl-specific effects and complex genetic interactions in Petunia hybrida flower development. Plant Cell (2004) 1.51

RNA silencing platforms in plants. FEBS Lett (2005) 1.50

Chalcone synthase as a reporter in virus-induced gene silencing studies of flower senescence. Plant Mol Biol (2004) 1.42

Elaboration of B gene function to include the identity of novel floral organs in the lower eudicot Aquilegia. Plant Cell (2007) 1.36

Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley. Plant Physiol (2005) 1.36

Virus induced gene silencing of a DEFICIENS ortholog in Nicotiana benthamiana. Plant Mol Biol (2004) 1.27

Convergence, constraint and the role of gene expression during adaptive radiation: floral anthocyanins in Aquilegia. Mol Ecol (2006) 1.27

Genetics of floral traits influencing reproductive isolation between Aquilegia formosa and Aquilegia pubescens. Am Nat (2002) 1.23

Transmission of tobacco rattle virus isolate PpK20 by its nematode vector requires one of the two non-structural genes in the viral RNA 2. J Gen Virol (1997) 0.90

Articles by these authors

Major flowering time gene, flowering locus C, regulates seed germination in Arabidopsis thaliana. Proc Natl Acad Sci U S A (2009) 1.87

Differential regulation of symmetry genes and the evolution of floral morphologies. Proc Natl Acad Sci U S A (2003) 1.52

Floral symmetry genes and the origin and maintenance of zygomorphy in a plant-pollinator mutualism. Proc Natl Acad Sci U S A (2010) 1.26

Evolution of the APETALA3 and PISTILLATA lineages of MADS-box-containing genes in the basal angiosperms. Mol Biol Evol (2003) 1.26

Are we there yet? Tracking the development of new model systems. Trends Genet (2008) 1.24

The ABC model and the diversification of floral organ identity. Semin Cell Dev Biol (2009) 1.19

Floral MADS box genes and homeotic gender dimorphism in Thalictrum dioicum (Ranunculaceae) - a new model for the study of dioecy. Plant J (2005) 1.04

Pleiotropy in the wild: the dormancy gene DOG1 exerts cascading control on life cycles. Evolution (2012) 1.02

Deep annotation of Populus trichocarpa microRNAs from diverse tissue sets. PLoS One (2012) 1.01

Developmental origins of the world's largest flowers, Rafflesiaceae. Proc Natl Acad Sci U S A (2013) 0.99

Disruption of the petal identity gene APETALA3-3 is highly correlated with loss of petals within the buttercup family (Ranunculaceae). Proc Natl Acad Sci U S A (2013) 0.96

Molecular evolution of the petal and stamen identity genes, APETALA3 and PISTILLATA, after petal loss in the Piperales. Mol Phylogenet Evol (2007) 0.95

Evolution of spur-length diversity in Aquilegia petals is achieved solely through cell-shape anisotropy. Proc Biol Sci (2011) 0.95

One size fits all? Molecular evidence for a commonly inherited petal identity program in Ranunculales. Am J Bot (2008) 0.94

APETALA3 and PISTILLATA homologs exhibit novel expression patterns in the unique perianth of Aristolochia (Aristolochiaceae). Evol Dev (2004) 0.94

In the Light of Evolution: A Reevaluation of Conservation in the CO-FT Regulon and Its Role in Photoperiodic Regulation of Flowering Time. Front Plant Sci (2011) 0.92

Petal-specific subfunctionalization of an APETALA3 paralog in the Ranunculales and its implications for petal evolution. New Phytol (2011) 0.91

Old dogs, new tricks: regulatory evolution in conserved genetic modules leads to novel morphologies in plants. Dev Biol (2009) 0.90

STENOFOLIA recruits TOPLESS to repress ASYMMETRIC LEAVES2 at the leaf margin and promote leaf blade outgrowth in Medicago truncatula. Plant Cell (2014) 0.89

Floral structure and development in Rafflesiaceae with emphasis on their exceptional gynoecia. Am J Bot (2014) 0.87

Within and between whorls: comparative transcriptional profiling of Aquilegia and Arabidopsis. PLoS One (2010) 0.86

Convergent evolution of a complex fruit structure in the tribe Brassiceae (Brassicaceae). Am J Bot (2011) 0.85

Similar genetic mechanisms underlie the parallel evolution of floral phenotypes. PLoS One (2012) 0.85

The corona of the daffodil Narcissus bulbocodium shares stamen-like identity and is distinct from the orthodox floral whorls. Plant J (2013) 0.85

The Aquilegia FRUITFULL-like genes play key roles in leaf morphogenesis and inflorescence development. Plant J (2013) 0.84

Divergent genetic mechanisms underlie reversals to radial floral symmetry from diverse zygomorphic flowered ancestors. Front Plant Sci (2013) 0.83

Identification of conserved Aquilegia coerulea microRNAs and their targets. Gene (2009) 0.82

Environmental and molecular analysis of the floral transition in the lower eudicot Aquilegia formosa. Evodevo (2011) 0.81

Understanding the basis of a novel fruit type in Brassicaceae: conservation and deviation in expression patterns of six genes. Evodevo (2012) 0.80

Virus-induced gene silencing in the rapid cycling columbine Aquilegia coerulea "Origami". Methods Mol Biol (2013) 0.78

Characterization of Aquilegia Polycomb Repressive Complex 2 homologs reveals absence of imprinting. Gene (2012) 0.78

The evolution of reproductive structures in seed plants: a re-examination based on insights from developmental genetics. New Phytol (2012) 0.78

Columbines. Curr Biol (2007) 0.77