Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion.

PubWeight™: 0.79‹?›

🔗 View Article (PMC 2258576)

Published in Appl Environ Microbiol on December 14, 2007

Authors

Kian Sim Goo1, Chun Song Chua, Tiow-Suan Sim

Author Affiliations

1: Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Singapore.

Articles cited by this

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res (1994) 392.47

LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions. Protein Eng (1995) 19.22

Protein modelling for all. Trends Biochem Sci (1999) 4.41

Additivity of mutational effects in proteins. Biochemistry (1990) 4.05

Structure of a cephalosporin synthase. Nature (1998) 2.59

Industrial production of beta-lactam antibiotics. Appl Microbiol Biotechnol (2003) 1.69

A twisted base? The role of arginine in enzyme-catalyzed proton abstractions. Arch Biochem Biophys (2005) 1.59

Quantitative interpretations of double mutations of enzymes. Arch Biochem Biophys (1992) 1.58

Studies on the active site of deacetoxycephalosporin C synthase. J Mol Biol (1999) 1.13

The structural basis of cephalosporin formation in a mononuclear ferrous enzyme. Nat Struct Mol Biol (2003) 1.12

The beta-lactam antibiotics: past, present, and future. Antonie Van Leeuwenhoek (1999) 1.12

Environmentally safe production of 7-aminodeacetoxycephalosporanic acid (7-ADCA) using recombinant strains of Acremonium chrysogenum. Nat Biotechnol (2000) 1.10

Potential use of additivity of mutational effects in simplifying protein engineering. Proc Natl Acad Sci U S A (1996) 1.02

Engineering Streptomyces clavuligerus deacetoxycephalosporin C synthase for optimal ring expansion activity toward penicillin G. Appl Environ Microbiol (2003) 1.01

Mutation of N304 to leucine in Streptomyces clavuligerus deacetoxycephalosporin C synthase creates an enzyme with increased penicillin analogue conversion. Biochem Biophys Res Commun (2001) 0.97

C-terminus modification of Streptomyces clavuligerus deacetoxycephalosporin C synthase improves catalysis with an expanded substrate specificity. Biochem Biophys Res Commun (2002) 0.96

Family shuffling of expandase genes to enhance substrate specificity for penicillin G. Appl Environ Microbiol (2004) 0.93

Structural and functional effects of multiple mutations at distal sites in cytochrome c. Biochemistry (1995) 0.90

The role of arginine residues in substrate binding and catalysis by deacetoxycephalosporin C synthase. Eur J Biochem (2002) 0.87

Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion. Appl Environ Microbiol (2005) 0.85

Probing the penicillin sidechain selectivity of recombinant deacetoxycephalosporin C synthase. Cell Mol Life Sci (2001) 0.84

Active site mutations of recombinant deacetoxycephalosporin C synthase. Biochem Biophys Res Commun (2002) 0.84

Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G. J Ind Microbiol Biotechnol (2003) 0.82

A complete library of amino acid alterations at N304 in Streptomyces clavuligerus deacetoxycephalosporin C synthase elucidates the basis for enhanced penicillin analogue conversion. Appl Environ Microbiol (2004) 0.82

A comparison of three site-directed mutagenesis kits. Z Naturforsch C (2001) 0.78

A complete library of amino acid alterations at R306 in Streptomyces clavuligerus deacetoxycephalosporin C synthase demonstrates its structural role in the ring-expansion activity. Proteins (2008) 0.77

Articles by these authors

Structural basis of the radicicol resistance displayed by a fungal hsp90. ACS Chem Biol (2009) 1.10

Pfnek3: an atypical activator of a MAP kinase in Plasmodium falciparum. FEBS Lett (2006) 0.88

Directed evolution and rational approaches to improving Streptomyces clavuligerus deacetoxycephalosporin C synthase for cephalosporin production. J Ind Microbiol Biotechnol (2009) 0.85

Features of the Streptomyces hygroscopicus HtpG reveal how partial geldanamycin resistance can arise with mutation to the ATP binding pocket of a eukaryotic Hsp90. FASEB J (2011) 0.85

Differences in biochemical properties of the Plasmodial falcipain-2 and berghepain-2 orthologues: implications for in vivo screens of inhibitors. FEMS Microbiol Lett (2005) 0.82

Pfnek3 functions as an atypical MAPKK in Plasmodium falciparum. Biochem Biophys Res Commun (2007) 0.81

Plasmodium falciparum possesses a unique dual-specificity serine/threonine and tyrosine kinase, Pfnek3. Cell Mol Life Sci (2011) 0.81

Designing new β-lactams: implications from their targets, resistance factors and synthesizing enzymes. Curr Comput Aided Drug Des (2011) 0.79

Elucidation of active site residues of Arabidopsis thaliana flavonol synthase provides a molecular platform for engineering flavonols. Phytochemistry (2007) 0.78

Atypical caseinolytic protease homolog from Plasmodium falciparum possesses unusual substrate preference and a functional nuclear localization signal. Parasitol Res (2009) 0.78

Functional analysis, overexpression, and kinetic characterization of pyruvate kinase from Plasmodium falciparum. Biochem Biophys Res Commun (2005) 0.78

A complete library of amino acid alterations at R306 in Streptomyces clavuligerus deacetoxycephalosporin C synthase demonstrates its structural role in the ring-expansion activity. Proteins (2008) 0.77

Molecular basis for thermal properties of Streptomyces thermovulgaris fumarase C hinge at hydrophilic amino acids R163, E170 and S347. Appl Microbiol Biotechnol (2007) 0.77

Regulation of Plasmodium falciparum Pfnek3 relies on phosphorylation at its activation loop and at threonine 82. Cell Mol Life Sci (2009) 0.76

Molecular cloning and functional characterisation of VanX, a D-alanyl-D-alanine dipeptidase from Streptomyces coelicolor A3(2). Res Microbiol (2002) 0.76

A relevant in vitro eukaryotic live-cell system for the evaluation of plasmodial protein localization. Biochimie (2006) 0.76