Published in BMC Res Notes on August 28, 2008
Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in The Netherlands. Appl Environ Microbiol (2011) 1.24
Methods for applying accurate digital PCR analysis on low copy DNA samples. PLoS One (2013) 1.21
Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification. Anal Bioanal Chem (2014) 1.08
Quantification of Shiga toxin-converting bacteriophages in wastewater and in fecal samples by real-time quantitative PCR. Appl Environ Microbiol (2010) 1.06
Zip Nucleic Acids: new high affinity oligonucleotides as potent primers for PCR and reverse transcription. Nucleic Acids Res (2009) 1.04
Detection of Mycobacterium tuberculosis in blood by use of the Xpert MTB/RIF assay. J Clin Microbiol (2013) 1.03
Targeted RNA-sequencing with competitive multiplex-PCR amplicon libraries. PLoS One (2013) 1.03
Implications of storing urinary DNA from different populations for molecular analyses. PLoS One (2009) 1.00
External quality assurance of malaria nucleic acid testing for clinical trials and eradication surveillance. PLoS One (2014) 0.92
DNA extract characterization process for microbial detection methods development and validation. BMC Res Notes (2012) 0.89
Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples. Anal Bioanal Chem (2014) 0.84
Optimization of PCR for quantification of simian immunodeficiency virus genomic RNA in plasma of rhesus macaques (Macaca mulatta) using armored RNA. J Med Primatol (2013) 0.84
Urine-based human papillomavirus DNA testing as a screening tool for cervical cancer in high-risk women. Int J Gynaecol Obstet (2013) 0.83
Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces. Toxins (Basel) (2016) 0.81
The detection of different Dirofilaria species using direct PCR technique. Parasitol Res (2013) 0.81
Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR. J Clin Microbiol (2012) 0.80
Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies. J Biomol Tech (2014) 0.79
qPCR, dPCR, NGS - A journey. Biomol Detect Quantif (2015) 0.79
Pressure cycling technology (PCT) reduces effects of inhibitors of the PCR. Int J Legal Med (2012) 0.79
Screening ancient tuberculosis with qPCR: challenges and opportunities. Philos Trans R Soc Lond B Biol Sci (2015) 0.78
Removal of Contaminant DNA by Combined UV-EMA Treatment Allows Low Copy Number Detection of Clinically Relevant Bacteria Using Pan-Bacterial Real-Time PCR. PLoS One (2015) 0.78
Multi-template polymerase chain reaction. Biomol Detect Quantif (2014) 0.78
Comparison of Droplet Digital PCR and Quantitative PCR Assays for Quantitative Detection of Xanthomonas citri Subsp. citri. PLoS One (2016) 0.77
Detection of BRAF Mutation in Urine DNA as a Molecular Diagnostic for Canine Urothelial and Prostatic Carcinoma. PLoS One (2015) 0.77
A comparison between quantitative PCR and droplet digital PCR technologies for circulating microRNA quantification in human lung cancer. BMC Biotechnol (2016) 0.77
Accurate detection and quantification of the fish viral hemorrhagic Septicemia virus (VHSv) with a two-color fluorometric real-time PCR assay. PLoS One (2013) 0.76
Potential Approaches and Recent Advances in Biomarker Discovery in Clear-Cell Renal Cell Carcinoma. J Cancer (2015) 0.76
PCR Inhibition of a Quantitative PCR for Detection of Mycobacterium avium Subspecies Paratuberculosis DNA in Feces: Diagnostic Implications and Potential Solutions. Front Microbiol (2017) 0.75
RNA isolation from precision-cut lung slices (PCLS) from different species. BMC Res Notes (2017) 0.75
RNomic identification and evaluation of npcTB_6715, a non-protein-coding RNA gene as a potential biomarker for the detection of Mycobacterium tuberculosis. J Cell Mol Med (2017) 0.75
Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR. PLoS One (2017) 0.75
Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res (2003) 77.89
Predicting DNA duplex stability from the base sequence. Proc Natl Acad Sci U S A (1986) 21.73
Quantification of mRNA using real-time RT-PCR. Nat Protoc (2006) 11.43
Real-time RT-PCR normalisation; strategies and considerations. Genes Immun (2005) 10.93
Quantitative real-time RT-PCR--a perspective. J Mol Endocrinol (2005) 6.72
Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J Biomol Tech (2004) 5.97
Standardized determination of real-time PCR efficiency from a single reaction set-up. Nucleic Acids Res (2003) 4.46
Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samples. Appl Environ Microbiol (1998) 2.93
Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clin Sci (Lond) (2005) 2.12
SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations. Anal Biochem (2006) 2.08
Evaluation of real-time PCR amplification efficiencies to detect PCR inhibitors. J Forensic Sci (2006) 1.79
Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences. Expert Rev Mol Diagn (2005) 1.57
Development and evaluation of a real-time PCR assay for detection of Pneumocystis jirovecii DNA in bronchoalveolar lavage fluid of HIV-infected patients. Thorax (2007) 1.39
Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection. Nucleic Acids Res (2005) 1.28
Impact of DNA polymerases and their buffer systems on quantitative real-time PCR. J Clin Microbiol (2004) 1.22
Development and evaluation of a real-time RT-PCR assay for quantification of cell-free human immunodeficiency virus type 2 using a Brome Mosaic Virus internal control. J Virol Methods (2006) 1.20
Exogenous reference RNA for normalization of real-time quantitative PCR. Biotechniques (2003) 1.19
PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency. Nucleic Acids Res (2005) 1.18
Application of a real-time polymerase chain reaction with internal positive control for detection and quantification of enterovirus in cerebrospinal fluid. Eur J Clin Microbiol Infect Dis (2002) 1.05
Genotypic analysis of the earliest known prehistoric case of tuberculosis in Britain. J Clin Microbiol (2005) 0.96
Evaluation of six methods for extraction and purification of viral DNA from urine and serum samples. New Microbiol (2006) 0.95
Matrine induces programmed cell death and regulates expression of relevant genes based on PCR array analysis in C6 glioma cells. Mol Biol Rep (2008) 0.94
The use of unprocessed urine samples for detecting and monitoring BK viruses in renal transplant recipients by a quantitative real-time PCR assay. J Virol Methods (2008) 0.80
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem (2009) 40.90
Immune reconstitution disease associated with mycobacterial infections in HIV-infected individuals receiving antiretrovirals. Lancet Infect Dis (2005) 6.59
Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques (2004) 6.42
Best drug treatment for multidrug-resistant and extensively drug-resistant tuberculosis. Lancet Infect Dis (2010) 5.14
Early treatment outcomes and HIV status of patients with extensively drug-resistant tuberculosis in South Africa: a retrospective cohort study. Lancet (2010) 4.95
Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet (2010) 4.71
Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study. Lancet (2009) 4.53
Biomarkers for tuberculosis disease activity, cure, and relapse. Lancet Infect Dis (2009) 4.14
Constrictive bronchiolitis in soldiers returning from Iraq and Afghanistan. N Engl J Med (2011) 3.58
Effect of two different house screening interventions on exposure to malaria vectors and on anaemia in children in The Gambia: a randomised controlled trial. Lancet (2009) 3.45
The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments. Clin Chem (2013) 3.38
Advances in tuberculosis diagnostics: the Xpert MTB/RIF assay and future prospects for a point-of-care test. Lancet Infect Dis (2013) 3.12
Innovative trial designs are practical solutions for improving the treatment of tuberculosis. J Infect Dis (2012) 3.09
Lung diseases at necropsy in African children dying from respiratory illnesses: a descriptive necropsy study. Lancet (2002) 2.92
Disease-associated XMRV sequences are consistent with laboratory contamination. Retrovirology (2010) 2.90
Immunological biomarkers of tuberculosis. Nat Rev Immunol (2011) 2.88
Scaling up interventions to achieve global tuberculosis control: progress and new developments. Lancet (2012) 2.83
Role of co-trimoxazole prophylaxis in reducing mortality in HIV infected adults being treated for tuberculosis: randomised clinical trial. BMJ (2008) 2.66
Use of dried whole blood spots to measure CD4+ lymphocyte counts in HIV-1-infected patients. Lancet (2003) 2.64
Efficacy, safety and tolerability of linezolid containing regimens in treating MDR-TB and XDR-TB: systematic review and meta-analysis. Eur Respir J (2012) 2.61
A new name (Pneumocystis jiroveci) for Pneumocystis from humans. Emerg Infect Dis (2002) 2.57
Within-subject variability and boosting of T-cell interferon-gamma responses after tuberculin skin testing. Am J Respir Crit Care Med (2009) 2.55
Risk factors for house-entry by malaria vectors in a rural town and satellite villages in The Gambia. Malar J (2008) 2.51
Early predictors of mortality from Pneumocystis jirovecii pneumonia in HIV-infected patients: 1985-2006. Clin Infect Dis (2008) 2.42
Rapid and accurate detection of Mycobacterium tuberculosis in sputum samples by Cepheid Xpert MTB/RIF assay--a clinical validation study. PLoS One (2011) 2.40
Performance of a T-cell-based diagnostic test for tuberculosis infection in HIV-infected individuals is independent of CD4 cell count. AIDS (2005) 2.38
Assessment of the Xpert MTB/RIF assay for diagnosis of tuberculosis with gastric lavage aspirates in children in sub-Saharan Africa: a prospective descriptive study. Lancet Infect Dis (2012) 2.29
Progress towards improved tuberculosis diagnostics for developing countries. Lancet (2006) 2.16
Drug-resistant tuberculosis: time for visionary political leadership. Lancet Infect Dis (2013) 2.16
Tuberculosis biomarkers discovery: developments, needs, and challenges. Lancet Infect Dis (2013) 2.05
Clinical utility of a commercial LAM-ELISA assay for TB diagnosis in HIV-infected patients using urine and sputum samples. PLoS One (2010) 2.03
Recognition of stage-specific mycobacterial antigens differentiates between acute and latent infections with Mycobacterium tuberculosis. Clin Vaccine Immunol (2006) 1.99
Chronic lung disease in adolescents with delayed diagnosis of vertically acquired HIV infection. Clin Infect Dis (2012) 1.90
Tuberculosis diagnostics and biomarkers: needs, challenges, recent advances, and opportunities. J Infect Dis (2012) 1.89
Microbial larvicides for malaria control in The Gambia. Malar J (2007) 1.78
Urine for the diagnosis of tuberculosis: current approaches, clinical applicability, and new developments. Curr Opin Pulm Med (2010) 1.73
Immune responses to tuberculosis in developing countries: implications for new vaccines. Nat Rev Immunol (2005) 1.72
Lung remodeling in pulmonary tuberculosis. J Infect Dis (2005) 1.71
Scale-up of services and research priorities for diagnosis, management, and control of tuberculosis: a call to action. Lancet (2010) 1.69
Severe acute respiratory syndrome vs. the Middle East respiratory syndrome. Curr Opin Pulm Med (2014) 1.69
Near-universal prevalence of Pneumocystis and associated increase in mucus in the lungs of infants with sudden unexpected death. Clin Infect Dis (2012) 1.64
Tuberculosis comorbidity with communicable and non-communicable diseases: integrating health services and control efforts. Lancet Infect Dis (2013) 1.62
Spatial distribution of mosquito larvae and the potential for targeted larval control in The Gambia. Am J Trop Med Hyg (2008) 1.61
Reversing the tide of the UK tuberculosis epidemic. Lancet (2013) 1.61
Neuroectodermal differentiation from mouse multipotent adult progenitor cells. Proc Natl Acad Sci U S A (2003) 1.59
D-serine and serine racemase are present in the vertebrate retina and contribute to the physiological activation of NMDA receptors. Proc Natl Acad Sci U S A (2003) 1.57
HIV infection presenting in older children and adolescents: a case series from Harare, Zimbabwe. Clin Infect Dis (2007) 1.56
BCG: the story continues. Lancet (2005) 1.55
Healthy individuals that control a latent infection with Mycobacterium tuberculosis express high levels of Th1 cytokines and the IL-4 antagonist IL-4delta2. J Immunol (2004) 1.48
Environmental risk factors for Pneumocystis pneumonia hospitalizations in HIV patients. Clin Infect Dis (2012) 1.48
New drugs for the treatment of tuberculosis: needs, challenges, promise, and prospects for the future. J Infect Dis (2012) 1.46
Monocyte derived dendritic cells from HIV-1 infected individuals partially reconstitute CD4 T-cell responses. AIDS (2006) 1.44
Whole blood versus plasma spots for measurement of HIV-1 viral load in HIV-infected African patients. Lancet (2003) 1.43
Tuberculosis in the UK--time to regain control. BMJ (2011) 1.43
Identification of Pneumocystis carinii DNA by polymerase chain reaction in necropsy lung samples from children dying of respiratory tract illnesses. J Pediatr (2002) 1.42
Biomarkers for tuberculosis disease activity, cure, and relapse. Lancet Infect Dis (2010) 1.41
Tuberculosis in prisons in sub-Saharan Africa--the need for improved health services, surveillance and control. Tuberculosis (Edinb) (2011) 1.40
New and improved diagnostics for detection of drug-resistant pulmonary tuberculosis. Curr Opin Pulm Med (2011) 1.40
Alignment of new tuberculosis drug regimens and drug susceptibility testing: a framework for action. Lancet Infect Dis (2013) 1.39
Quantitative imaging assay for NF-kappaB nuclear translocation in primary human macrophages. J Immunol Methods (2007) 1.35
HIV-1 infection of macrophages is dependent on evasion of innate immune cellular activation. AIDS (2009) 1.34
Clinical diagnostic utility of IP-10 and LAM antigen levels for the diagnosis of tuberculous pleural effusions in a high burden setting. PLoS One (2009) 1.34
Primer sequence disclosure: a clarification of the MIQE guidelines. Clin Chem (2011) 1.32
Proteomic patterns of preinvasive bronchial lesions. Am J Respir Crit Care Med (2005) 1.32
Effect of sample handling on analysis of cytokine responses to Mycobacterium tuberculosis in clinical samples using ELISA, ELISPOT and quantitative PCR. J Immunol Methods (2005) 1.29
HIV-associated Pneumocystis pneumonia. Proc Am Thorac Soc (2011) 1.29
Is mosquito larval source management appropriate for reducing malaria in areas of extensive flooding in The Gambia? A cross-over intervention trial. Am J Trop Med Hyg (2010) 1.29
Detection of SARS coronavirus in plasma by real-time RT-PCR. N Engl J Med (2003) 1.28
Evaluation of the Xpert MTB/RIF assay at a tertiary care referral hospital in a setting where tuberculosis and HIV infection are highly endemic. Clin Infect Dis (2012) 1.28
Different screening strategies (single or dual) for the diagnosis of suspected latent tuberculosis: a cost effectiveness analysis. BMC Pulm Med (2010) 1.23
Extensively drug-resistant tuberculosis: epidemiology and management challenges. Infect Dis Clin North Am (2010) 1.23
Tuberculosis control is crucial to achieve the MDGs. Lancet (2010) 1.22
HIV-1 infection of mononuclear phagocytic cells: the case for bacterial innate immune deficiency in AIDS. Lancet Infect Dis (2006) 1.21
Skin disease among human immunodeficiency virus-infected adolescents in Zimbabwe: a strong indicator of underlying HIV infection. Pediatr Infect Dis J (2010) 1.20
Tuberculosis drug discovery in the post-post-genomic era. EMBO Mol Med (2014) 1.20
The resurgence of disease: social and historical perspectives on the 'new' tuberculosis. Soc Sci Med (2002) 1.19
How great is the risk of Middle East respiratory syndrome coronavirus to the global population? Expert Rev Anti Infect Ther (2013) 1.19
Interaction between HIV and Mycobacterium tuberculosis: HIV-1-induced CD4 T-cell depletion and the development of active tuberculosis. Curr Opin HIV AIDS (2012) 1.18
Variation in gamma interferon responses to different infecting strains of Mycobacterium tuberculosis in acid-fast bacillus smear-positive patients and household contacts in Antananarivo, Madagascar. Clin Vaccine Immunol (2010) 1.17
Limited asymptomatic carriage of Pneumocystis jiroveci in human immunodeficiency virus-infected patients. J Infect Dis (2003) 1.17
Progress and challenges in childhood tuberculosis. Lancet Infect Dis (2013) 1.16
Increased expression of mRNA encoding interleukin (IL)-4 and its splice variant IL-4delta2 in cells from contacts of Mycobacterium tuberculosis, in the absence of in vitro stimulation. Immunology (2004) 1.15
Neuroserpin binds Abeta and is a neuroprotective component of amyloid plaques in Alzheimer disease. J Biol Chem (2006) 1.14
Endogenous D-serine contributes to NMDA-receptor-mediated light-evoked responses in the vertebrate retina. J Neurophysiol (2007) 1.14
Thoracic empyema: current opinions in medical and surgical management. Curr Opin Pulm Med (2010) 1.14
Extensively drug-resistant tuberculosis (XDR-TB) among health care workers in South Africa. Trop Med Int Health (2010) 1.11
Review of multidrug-resistant and extensively drug-resistant TB: global perspectives with a focus on sub-Saharan Africa. Trop Med Int Health (2010) 1.10
Tuberculosis in prisons in sub-Saharan Africa--a potential time bomb. S Afr Med J (2011) 1.10
High rates of congenital cytomegalovirus infection linked with maternal HIV infection among neonatal admissions at a large referral center in sub-Saharan Africa. Clin Infect Dis (2013) 1.10
In vivo and in vitro studies of a novel cytokine, interleukin 4delta2, in pulmonary tuberculosis. Am J Respir Crit Care Med (2005) 1.10
Biomarkers for tuberculosis disease status and diagnosis. Curr Opin Pulm Med (2009) 1.09
Tuberculosis in association with HIV/AIDS emerges as a major nonobstetric cause of maternal mortality in Sub-Saharan Africa. Int J Gynaecol Obstet (2010) 1.08
Mycobacterium tuberculosis Antigen 85A induces Th-1 immune responses in systemic sarcoidosis. J Clin Immunol (2007) 1.08