Novel Parachlamydia acanthamoebae quantification method based on coculture with amoebae.

PubWeight™: 0.98‹?› | Rank: Top 15%

🔗 View Article (PMC 2570282)

Published in Appl Environ Microbiol on August 29, 2008

Authors

Junji Matsuo1, Yasuhiro Hayashi, Shinji Nakamura, Marie Sato, Yoshihiko Mizutani, Masahiro Asaka, Hiroyuki Yamaguchi

Author Affiliations

1: Department of Medical Laboratory Sciences, Faculty of Health Sciences, Hokkaido University, Sapporo, Hokkaido, Japan.

Articles citing this

Amoebal endosymbiont Neochlamydia genome sequence illuminates the bacterial role in the defense of the host amoebae against Legionella pneumophila. PLoS One (2014) 0.91

Ciliates expel environmental Legionella-laden pellets to stockpile food. Appl Environ Microbiol (2012) 0.84

Acanthamoeba polyphaga mimivirus and other giant viruses: an open field to outstanding discoveries. Virol J (2014) 0.84

Environmental chlamydiae alter the growth speed and motility of host acanthamoebae. Microbes Environ (2012) 0.82

Impact of free-living amoebae on presence of Parachlamydia acanthamoebae in the hospital environment and its survival in vitro without requirement for amoebae. J Clin Microbiol (2010) 0.81

Protochlamydia induces apoptosis of human HEp-2 cells through mitochondrial dysfunction mediated by chlamydial protease-like activity factor. PLoS One (2013) 0.80

Amoebal endosymbiont Protochlamydia induces apoptosis to human immortal HEp-2 cells. PLoS One (2012) 0.79

Amoebal endosymbiont Parachlamydia acanthamoebae Bn9 can grow in immortal human epithelial HEp-2 cells at low temperature; an in vitro model system to study chlamydial evolution. PLoS One (2015) 0.78

Draft Genome Sequence of High-Temperature-Adapted Protochlamydia sp. HS-T3, an Amoebal Endosymbiotic Bacterium Found in Acanthamoeba Isolated from a Hot Spring in Japan. Genome Announc (2015) 0.77

Evolutionary conservation of infection-induced cell death inhibition among Chlamydiales. PLoS One (2011) 0.76

Acanthamoeba containing endosymbiotic chlamydia isolated from hospital environments and its potential role in inflammatory exacerbation. BMC Microbiol (2016) 0.75

Articles cited by this

Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells. J Clin Microbiol (1977) 11.91

Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol Rev (2006) 3.96

Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis. Infect Immun (1996) 3.67

Use of HEp-2 cells for improved isolation and passage of Chlamydia pneumoniae. J Clin Microbiol (1992) 3.10

The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J Eukaryot Microbiol (1998) 3.02

Replication of Chlamydia pneumoniae in vitro in human macrophages, endothelial cells, and aortic artery smooth muscle cells. Infect Immun (1996) 2.52

Cultivation of pathogenic and opportunistic free-living amebas. Clin Microbiol Rev (2002) 2.29

Obligate intracellular bacterial parasites of acanthamoebae related to Chlamydia spp. Appl Environ Microbiol (1997) 2.27

Ecology of free-living amoebae. Crit Rev Microbiol (1994) 2.14

The intracellular life of Chlamydia. Curr Top Microbiol Immunol (1988) 2.09

Electron Microscopic Observations on the Fine Structure of Cell Walls of Chlamydia psittaci. J Bacteriol (1970) 2.04

Crescent bodies of Parachlamydia acanthamoeba and its life cycle within Acanthamoeba polyphaga: an electron micrograph study. Appl Environ Microbiol (2002) 1.92

The fine structure of Acanthamoeba castellanii. I. The trophozoite. J Cell Biol (1968) 1.90

Chlamydia-like obligate parasite of free-living amoebae. Lancet (1997) 1.88

Legionella-like and other amoebal pathogens as agents of community-acquired pneumonia. Emerg Infect Dis (2001) 1.80

The increasing importance of Acanthamoeba infections. J Eukaryot Microbiol (2000) 1.78

Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates. J Med Microbiol (2005) 1.69

The late chlamydial inclusion membrane is not derived from the endocytic pathway and is relatively deficient in host proteins. Infect Immun (1996) 1.66

Pathogenic potential of novel Chlamydiae and diagnostic approaches to infections due to these obligate intracellular bacteria. Clin Microbiol Rev (2006) 1.62

Pathogenesis of Acanthamoeba infections. Microb Pathog (2003) 1.62

Isolation of a previously undescribed rickettsia from an aborted bovine fetus. J Clin Microbiol (1990) 1.58

Epizootiology of Chlamydia infections in two free-range koala populations. Vet Microbiol (1999) 1.58

Molecular evidence for the existence of additional members of the order Chlamydiales. Microbiology (1999) 1.57

Molecular evolution of the Chlamydiaceae. Int J Syst Evol Microbiol (2001) 1.57

Intracellular survival and saprophytic growth of isolates from the Burkholderia cepacia complex in free-living amoebae. Microbiology (1999) 1.53

Analysis of the 16S rRNA gene of micro-organism WSU 86-1044 from an aborted bovine foetus reveals that it is a member of the order Chlamydiales: proposal of Waddliaceae fam. nov., Waddlia chondrophila gen. nov., sp. nov. Int J Syst Bacteriol (1999) 1.52

Identification of MEK- and phosphoinositide 3-kinase-dependent signalling as essential events during Chlamydia pneumoniae invasion of HEp2 cells. Cell Microbiol (2002) 1.50

Novel bacterial endosymbionts of Acanthamoeba spp. related to the Paramecium caudatum symbiont Caedibacter caryophilus. Environ Microbiol (1999) 1.50

Parachlamydia acanthamoebae enters and multiplies within human macrophages and induces their apoptosis [corrected]. Infect Immun (2003) 1.47

Chlamydiae as pathogens: new species and new issues. Emerg Infect Dis (1997) 1.43

Pneumonia with serological evidence of acute infection with the Chlamydia-like microorganism "Z". Am J Respir Crit Care Med (1997) 1.42

Phylogenetic diversity among geographically dispersed Chlamydiales endosymbionts recovered from clinical and environmental isolates of Acanthamoeba spp. Appl Environ Microbiol (2000) 1.41

Evidence for Chlamydia pneumoniae of non-human origin. J Gen Microbiol (1993) 1.40

Serological hint suggesting that Parachlamydiaceae are agents of pneumonia in polytraumatized intensive care patients. Ann N Y Acad Sci (2003) 1.38

Electron microscope observations on the effects of polymixin B sulfate on cell walls of Chlamydia psittaci. J Bacteriol (1973) 1.38

Parachlamydiaceae as rare agents of pneumonia. Emerg Infect Dis (2003) 1.35

The prevalence of Acanthamoeba in the human environment. Rev Infect Dis (1991) 1.30

Chlamydia pneumoniae infection in human monocytes. Infect Immun (1999) 1.28

Characterization and intracellular trafficking pattern of vacuoles containing Chlamydia pneumoniae in human epithelial cells. Cell Microbiol (1999) 1.27

Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect Immun (1996) 1.20

Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells. Cell Microbiol (2005) 1.20

New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples. J Clin Microbiol (2008) 1.14

New parachlamydial 16S rDNA phylotypes detected in human clinical samples. Res Microbiol (2002) 1.13

Heparin-binding outer membrane protein of chlamydiae. Mol Microbiol (2001) 1.13

Chlamydia pneumoniae and atherosclerosis. Semin Respir Infect (2003) 1.13

Parachlamydia acanthamoeba is endosymbiotic or lytic for Acanthamoeba polyphaga depending on the incubation temperature. Ann N Y Acad Sci (2003) 1.10

Development of an Acanthamoeba-specific reverse dot-blot and the discovery of a new ribotype. J Eukaryot Microbiol (2002) 1.08

Detection of Chlamydia pneumoniae DNA and antigen in the circulating mononuclear cell fractions of humans and koalas. Infect Immun (2000) 1.02

Parachlamydia acanthamoebae enters and multiplies within pneumocytes and lung fibroblasts. Microbes Infect (2006) 1.01

Intracellular trafficking of Parachlamydia acanthamoebae. Cell Microbiol (2005) 0.98

Resistance of different Chlamydia-like organisms to quinolones and mutations in the quinoline resistance-determining region of the DNA gyrase A- and topoisomerase-encoding genes. Int J Antimicrob Agents (2006) 0.93

Legionella-like slender rods multiplying within a strain of Acanthamoeba sp. isolated from drinking water. Parasitol Res (1998) 0.92

Chlamydia pneumoniae infects and multiplies in lymphocytes in vitro. Infect Immun (2001) 0.90

Molecular characterization of Chlamydophila pneumoniae isolates from Western barred bandicoots. J Med Microbiol (2007) 0.87

Antibiotic susceptibilities of Parachlamydia acanthamoeba in amoebae. Antimicrob Agents Chemother (2002) 0.86

Murine model of pneumonia caused by Parachlamydia acanthamoebae. Microb Pathog (2008) 0.82

Lack of microbicidal response in human macrophages infected with Parachlamydia acanthamoebae. Microbes Infect (2005) 0.81

Enlarged Chlamydia-like organisms as spontaneous infection of Acanthamoeba castellanii. Parasitol Res (2001) 0.80

Chlamydia pneumoniae growth inhibition in human monocytic THP-1 cells and human epithelial HEp-2 cells by a novel phenoxazine derivative. J Med Microbiol (2005) 0.79

Response curves for radioimmunoassay. Clin Chem (1983) 0.76

Articles by these authors

Effect of eradication of Helicobacter pylori on incidence of metachronous gastric carcinoma after endoscopic resection of early gastric cancer: an open-label, randomised controlled trial. Lancet (2008) 7.24

SHP-2 tyrosine phosphatase as an intracellular target of Helicobacter pylori CagA protein. Science (2001) 5.49

Clinical significance and therapeutic potential of the programmed death-1 ligand/programmed death-1 pathway in human pancreatic cancer. Clin Cancer Res (2007) 4.41

Clinical features of hepatocellular carcinoma with extrahepatic metastases. J Gastroenterol Hepatol (2005) 3.51

Biological activity of the Helicobacter pylori virulence factor CagA is determined by variation in the tyrosine phosphorylation sites. Proc Natl Acad Sci U S A (2002) 3.50

Long-term clinical outcome of gastric MALT lymphoma after eradication of Helicobacter pylori: a multicentre cohort follow-up study of 420 patients in Japan. Gut (2011) 2.10

An irradiation-free nonmyeloablative bone marrow transplantation model: importance of the balance between donor T-cell number and the intensity of conditioning. Transplantation (2005) 2.08

Downregulation and growth inhibitory effect of epithelial-type Krüppel-like transcription factor KLF4, but not KLF5, in bladder cancer. Biochem Biophys Res Commun (2003) 2.01

Endoscopic nasobiliary drainage is the most suitable preoperative biliary drainage method in the management of patients with hilar cholangiocarcinoma. J Gastroenterol (2010) 1.98

Relationship between histopathologic gastritis and mucosal microvascularity: observations with magnifying endoscopy. Gastrointest Endosc (2003) 1.96

Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutations. Hepatology (2003) 1.87

Expression profiling in multistage hepatocarcinogenesis: identification of HSP70 as a molecular marker of early hepatocellular carcinoma. Hepatology (2003) 1.85

Rikkunshito, an herbal medicine, suppresses cisplatin-induced anorexia in rats via 5-HT2 receptor antagonism. Gastroenterology (2008) 1.83

Design and planned analyses of an ongoing randomized trial assessing the preventive effect of Helicobacter pylori eradication on occurrence of new gastric carcinomas after endoscopic resection. Helicobacter (2006) 1.67

Characteristics of magnified endoscopic images of gastric extranodal marginal zone B-cell lymphoma of the mucosa-associated lymphoid tissue, including changes after treatment. Gastrointest Endosc (2008) 1.67

Long-term outcome after endoscopic mucosal resection in patients with esophageal squamous cell carcinoma invading the muscularis mucosae or deeper. Gastrointest Endosc (2002) 1.65

Geographic differences in gastric cancer incidence can be explained by differences between Helicobacter pylori strains. Intern Med (2008) 1.62

Amelioration of dextran sulfate sodium-induced colitis by anti-macrophage migration inhibitory factor antibody in mice. Gastroenterology (2002) 1.54

Germline polymorphism of p53 codon 72 in gynecological cancer. Gynecol Oncol (2005) 1.49

Randomized study of Helicobacter pylori eradication therapy and proton pump inhibitor monotherapy for idiopathic thrombocytopenic purpura. Ann Hematol (2005) 1.48

Hepatocellular carcinoma in a male patient with early stage (stage I) primary biliary cirrhosis. Intern Med (2005) 1.48

Rapid degradation of Cdt1 upon UV-induced DNA damage is mediated by SCFSkp2 complex. J Biol Chem (2004) 1.46

[Expression of estrogen receptors alpha and beta, androgen receptors and progesterone receptors in human cornea]. Nippon Ganka Gakkai Zasshi (2002) 1.45

Dominant-negative hypoxia-inducible factor-1 alpha reduces tumorigenicity of pancreatic cancer cells through the suppression of glucose metabolism. Am J Pathol (2003) 1.45

ZAPS is a potent stimulator of signaling mediated by the RNA helicase RIG-I during antiviral responses. Nat Immunol (2010) 1.43

Primary paraganglioma of the head of pancreas: contribution of combinatorial image analyses to the diagnosis of disease. Intern Med (2005) 1.40

Overexpression of cortactin is involved in motility and metastasis of hepatocellular carcinoma. J Hepatol (2004) 1.37

Endoscopic diagnosis of early squamous neoplasia of the esophagus with iodine staining: high-grade intra-epithelial neoplasia turns pink within a few minutes. J Gastroenterol Hepatol (2007) 1.33

Endoscopic submucosal dissection for treatment of early stage hypopharyngeal carcinoma. Gastrointest Endosc (2006) 1.28

Effects of Helicobacter pylori CagA protein on the growth and survival of B lymphocytes, the origin of MALT lymphoma. Oncogene (2003) 1.25

Enhanced expression of asparagine synthetase under glucose-deprived conditions protects pancreatic cancer cells from apoptosis induced by glucose deprivation and cisplatin. Cancer Res (2007) 1.23

Progressive disappearance of anti-hepatitis B surface antigen antibody and reverse seroconversion after allogeneic hematopoietic stem cell transplantation in patients with previous hepatitis B virus infection. Transplantation (2005) 1.22

The seventh pathogenic fusion gene FIP1L1-RARA was isolated from a t(4;17)-positive acute promyelocytic leukemia. Haematologica (2008) 1.21

Eradication of gastric cancer and more efficient gastric cancer surveillance in Japan: two peas in a pod. J Gastroenterol (2009) 1.19

The effect of probiotic treatment with Clostridium butyricum on enterohemorrhagic Escherichia coli O157:H7 infection in mice. FEMS Immunol Med Microbiol (2004) 1.19

High salt diets dose-dependently promote gastric chemical carcinogenesis in Helicobacter pylori-infected Mongolian gerbils associated with a shift in mucin production from glandular to surface mucous cells. Int J Cancer (2006) 1.18

Minilaparotomy approach for removal of a large colonic lipoma: report of two cases. Surg Today (2004) 1.17

Reactivation of hepatitis B virus with rituximab. Expert Opin Drug Saf (2005) 1.17

Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability. Biochem Biophys Res Commun (2005) 1.13

The influence of hepatitis B DNA level and antiviral therapy on recurrence after initial curative treatment in patients with hepatocellular carcinoma. J Gastroenterol (2009) 1.13

Clinicopathological analysis of early-stage gastric cancers detected after successful eradication of Helicobacter pylori. Helicobacter (2011) 1.12

Spontaneous resolution of cap polyposis: case report. Gastrointest Endosc (2003) 1.12

Direct targeting of fibroblast growth factor-inducible 14 protein protects against renal ischemia reperfusion injury. Kidney Int (2010) 1.11

RNF43 interacts with NEDL1 and regulates p53-mediated transcription. Biochem Biophys Res Commun (2010) 1.11

Preliminary trial of rebamipide for prevention of low-dose aspirin-induced gastric injury in healthy subjects: a randomized, double-blind, placebo-controlled, cross-over study. J Clin Biochem Nutr (2009) 1.10

Monitoring of plasma imatinib concentration for the effective treatment of CML patients. Leuk Res (2004) 1.09

Systemic delivery of siRNA to tumors using a lipid nanoparticle containing a tumor-specific cleavable PEG-lipid. Biomaterials (2011) 1.09

Adrenomedullin antagonist suppresses in vivo growth of human pancreatic cancer cells in SCID mice by suppressing angiogenesis. Oncogene (2003) 1.08

IgG4-related sclerosing cholangitis and autoimmune pancreatitis: histological assessment of biopsies from Vater's ampulla and the bile duct. J Gastroenterol Hepatol (2010) 1.08

What is an adequate management strategy for pharyngeal low-grade dysplasia? Gastrointest Endosc (2013) 1.08

Role of the IL-6 classic- and trans-signaling pathways in corneal sterile inflammation and wound healing. Invest Ophthalmol Vis Sci (2011) 1.08

Identification and characterization of two Streptomyces davawensis riboflavin biosynthesis gene clusters. Arch Microbiol (2007) 1.07

Transforming mutations of RAC guanosine triphosphatases in human cancers. Proc Natl Acad Sci U S A (2013) 1.07

Side effects and good effects from new chemotherapeutic agents. Case 2. Thalidomide-induced interstitial pneumonitis. J Clin Oncol (2005) 1.06

Clinical role of (18)F-FDG PET for initial staging of patients with extrahepatic bile duct cancer. Eur J Nucl Med Mol Imaging (2002) 1.06

TRIM29 negatively regulates p53 via inhibition of Tip60. Biochim Biophys Acta (2011) 1.06

Autoimmune pancreatitis associated with hemorrhagic pseudocysts: a case report and literature review. Intern Med (2008) 1.06

A novel FRET-based biosensor for the measurement of BCR-ABL activity and its response to drugs in living cells. Clin Cancer Res (2010) 1.04

Multi-center randomized controlled study to establish the standard third-line regimen for Helicobacter pylori eradication in Japan. J Gastroenterol (2013) 1.03

Eicosapentaenoic acid ameliorates diabetic nephropathy of type 2 diabetic KKAy/Ta mice: involvement of MCP-1 suppression and decreased ERK1/2 and p38 phosphorylation. Nephrol Dial Transplant (2005) 1.03

Adjuvant photodynamic therapy for bile duct carcinoma after surgery: a preliminary study. J Gastroenterol (2004) 1.01

Hepatitis B virus reactivation in a case of non-Hodgkin's lymphoma treated with chemotherapy and rituximab: necessity of prophylaxis for hepatitis B virus reactivation in rituximab therapy. Leuk Lymphoma (2004) 1.00

Thrombotic microangiopathy after treatment with bortezomib and dexamethasone in a patient with multiple myeloma. Int J Hematol (2008) 1.00

Allogeneic bone marrow transplantation from an unrelated donor for the treatment of chronic myelogenous leukemia in blast crisis in a patient with Kleinfelter's syndrome. Leuk Lymphoma (2004) 1.00

The feasibility of Cep55/c10orf3 derived peptide vaccine therapy for colorectal carcinoma. Exp Mol Pathol (2010) 0.99

Synthesis and properties of BF2 complexes to dihydroxydiones of tetracene and perylene: novel electron acceptors showing n-type semiconducting behavior. Org Lett (2009) 0.99

Recent development of gastric cancer prevention. Jpn J Clin Oncol (2012) 0.99

Heat shock protein DNAJB8 is a novel target for immunotherapy of colon cancer-initiating cells. Cancer Sci (2014) 0.99

Ciliates rapidly enhance the frequency of conjugation between Escherichia coli strains through bacterial accumulation in vesicles. Res Microbiol (2010) 0.98

Three cases of retroperitoneal schwannoma diagnosed by EUS-FNA. World J Gastroenterol (2011) 0.98

Rikkunshito ameliorates the aging-associated decrease in ghrelin receptor reactivity via phosphodiesterase III inhibition. Endocrinology (2009) 0.98

Identification and characterization of novel cell wall hydrolase CwlT: a two-domain autolysin exhibiting n-acetylmuramidase and DL-endopeptidase activities. J Biol Chem (2008) 0.98

Analysis of intratumor heterogeneity of EGFR mutations in mixed type lung adenocarcinoma. Clin Lung Cancer (2013) 0.98

Importance of peroxisome proliferator-activated receptor-gamma in hepatic ischemia/reperfusion injury in mice. J Hepatol (2007) 0.97

Correlation between vascular endothelial growth factor-C expression and invasion phenotype in cervical carcinomas. Int J Cancer (2002) 0.97

Delayed reduction in left ventricular function following treatment of non-Hodgkin's lymphoma with chemotherapy and rituximab, unrelated to acute infusion reaction. Cardiology (2006) 0.97

Vascular endothelial growth factor-C expression and invasive phenotype in ovarian carcinomas. Clin Cancer Res (2005) 0.96

Relationship between CT volumetry and functional liver volume using technetium-99m galactosyl serum albumin scintigraphy in patients undergoing preoperative portal vein embolization before major hepatectomy: a preliminary study. Dig Dis Sci (2006) 0.96

Calcium/calmodulin-dependent protein kinase II regulates the phosphorylation of CREB in NMDA-induced retinal neurotoxicity. Brain Res (2007) 0.95

Decreased plasma ghrelin contributes to anorexia following novelty stress. Am J Physiol Endocrinol Metab (2011) 0.95

Heterogeneity of epidermal growth factor receptor mutations within a mixed adenocarcinoma lung nodule. Lung Cancer (2007) 0.95

Ro52 functionally interacts with IgG1 and regulates its quality control via the ERAD system. Mol Immunol (2007) 0.95

Homeobox gene expression and mutation in cervical carcinoma cells. Cancer Sci (2003) 0.95

Administration of micafungin as prophylactic antifungal therapy in patients undergoing allogeneic stem cell transplantation. Int J Hematol (2007) 0.95

Gastric MALT lymphomas are divided into three groups based on responsiveness to Helicobacter Pylori eradication and detection of API2-MALT1 fusion. Am J Surg Pathol (2004) 0.94

Synergistic up-regulation of Hexokinase-2, glucose transporters and angiogenic factors in pancreatic cancer cells by glucose deprivation and hypoxia. Exp Cell Res (2007) 0.94

EMR combined with chemoradiotherapy: a novel treatment for superficial esophageal squamous-cell carcinoma. Gastrointest Endosc (2004) 0.94

Prognostic value of immunohistochemical analysis of tumor budding in colorectal carcinoma. Anticancer Res (2008) 0.94

Relationship between diffusion-weighted magnetic resonance imaging and histological tumor grading of hepatocellular carcinoma. Ann Surg Oncol (2011) 0.94

p53 transactivation is involved in the antiproliferative activity of the putative tumor suppressor RBM5. Int J Cancer (2010) 0.94

Evaluation of the effects of strain-specific antigen variation on the accuracy of serologic diagnosis of Helicobacter pylori infection. J Clin Microbiol (2003) 0.94

Experimental infection of germ-free mice with hyper-toxigenic enterohaemorrhagic Escherichia coli O157:H7, strain 6. J Med Microbiol (2002) 0.94

6,11-Dihydroxy-naphthacene-5,12-dione. Acta Crystallogr Sect E Struct Rep Online (2007) 0.94

Laparoscopic lateral pelvic lymph node dissection for lower rectal cancer: initial clinical experiences with prophylactic dissection. Gan To Kagaku Ryoho (2012) 0.94

Impaired ghrelin signaling is associated with gastrointestinal dysmotility in rats with gastroesophageal reflux disease. Am J Physiol Gastrointest Liver Physiol (2012) 0.93

Fascin is involved in tumor necrosis factor-alpha-dependent production of MMP9 in cholangiocarcinoma. Lab Invest (2009) 0.93

Survivin gene expression in endometriosis. J Clin Endocrinol Metab (2002) 0.93