Published in FEBS J on February 18, 2009
Novel approaches to inhibiting HIV-1 replication. Antiviral Res (2009) 1.48
A Computational Model for Predicting RNase H Domain of Retrovirus. PLoS One (2016) 1.39
RNase H2 roles in genome integrity revealed by unlinking its activities. Nucleic Acids Res (2013) 1.19
Crystal structures of RNase H2 in complex with nucleic acid reveal the mechanism of RNA-DNA junction recognition and cleavage. Mol Cell (2010) 1.15
HIV-1 Ribonuclease H: Structure, Catalytic Mechanism and Inhibitors. Viruses (2010) 1.06
Engineering RNA endonucleases with customized sequence specificities. Nat Commun (2012) 1.01
HIV-1 Reverse Transcriptase Still Remains a New Drug Target: Structure, Function, Classical Inhibitors, and New Inhibitors with Innovative Mechanisms of Actions. Mol Biol Int (2012) 1.00
Understanding the effect of magnesium ion concentration on the catalytic activity of ribonuclease H through computation: does a third metal binding site modulate endonuclease catalysis? J Am Chem Soc (2010) 0.92
A protein ballet around the viral genome orchestrated by HIV-1 reverse transcriptase leads to an architectural switch: from nucleocapsid-condensed RNA to Vpr-bridged DNA. Virus Res (2012) 0.83
Preferred sequences within a defined cleavage window specify DNA 3' end-directed cleavages by retroviral RNases H. J Biol Chem (2009) 0.79
HIV-1 Reverse Transcriptase Polymerase and RNase H Active Sites Work Simultaneously and Independently. J Biol Chem (2016) 0.79
Deletion analysis of the 3' long terminal repeat sequence of plant retrotransposon Tto1 identifies 125 base pairs redundancy as sufficient for first strand transfer. Virology (2011) 0.79
Multiple nucleotide preferences determine cleavage-site recognition by the HIV-1 and M-MuLV RNases H. J Mol Biol (2010) 0.79
The removal of RNA primers from DNA synthesized by the reverse transcriptase of the retrotransposon Tf1 is stimulated by Tf1 integrase. J Virol (2012) 0.78
Sequence and generation of mature ribosomal RNA transcripts in Dictyostelium discoideum. J Biol Chem (2011) 0.77
Measuring specificity in multi-substrate/product systems as a tool to investigate selectivity in vivo. Biochim Biophys Acta (2015) 0.76
Ultradeep pyrosequencing and molecular modeling identify key structural features of hepatitis B virus RNase H, a putative target for antiviral intervention. J Virol (2013) 0.76
BARE retrotransposons are translated and replicated via distinct RNA pools. PLoS One (2013) 0.76
SiRNA-induced mutation in HIV-1 polypurine tract region and its influence on viral fitness. PLoS One (2015) 0.75
Arm-specific cleavage and mutation during reverse transcription of 2΄,5΄-branched RNA by Moloney murine leukemia virus reverse transcriptase. Nucleic Acids Res (2017) 0.75
RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature (1970) 19.89
RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature (1970) 19.38
Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance. Science (1998) 11.23
Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA. Proc Natl Acad Sci U S A (1993) 9.47
A detailed model of reverse transcription and tests of crucial aspects. Cell (1979) 8.93
Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell (2005) 5.43
Crystal structure of the ribonuclease H domain of HIV-1 reverse transcriptase. Science (1991) 5.42
Mechanism and fidelity of HIV reverse transcriptase. J Biol Chem (1992) 4.57
Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA. EMBO J (2001) 4.36
Making and breaking nucleic acids: two-Mg2+-ion catalysis and substrate specificity. Mol Cell (2006) 4.26
Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids. Nat New Biol (1971) 3.95
Structure of ribonuclease H phased at 2 A resolution by MAD analysis of the selenomethionyl protein. Science (1990) 3.77
Domain structure of the Moloney murine leukemia virus reverse transcriptase: mutational analysis and separate expression of the DNA polymerase and RNase H activities. Proc Natl Acad Sci U S A (1988) 3.02
Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription. Mol Cell (2007) 2.71
Human immunodeficiency virus type 1 reverse transcriptase: spatial and temporal relationship between the polymerase and RNase H activities. Proc Natl Acad Sci U S A (1992) 2.64
Conferring RNA polymerase activity to a DNA polymerase: a single residue in reverse transcriptase controls substrate selection. Proc Natl Acad Sci U S A (1997) 2.54
HIV-1 RT-associated ribonuclease H displays both endonuclease and 3'----5' exonuclease activity. EMBO J (1990) 2.54
Reverse transcriptase.RNase H from the human immunodeficiency virus. Relationship of the DNA polymerase and RNA hydrolysis activities. J Biol Chem (1991) 2.36
Interaction of HIV-1 ribonuclease H with polypurine tract containing RNA-DNA hybrids. Biochemistry (1990) 2.29
Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release. EMBO J (2006) 2.23
Human immunodeficiency virus reverse transcriptase ribonuclease H: specificity of tRNA(Lys3)-primer excision. Biochemistry (1991) 1.88
Mutations in the RNase H domain of HIV-1 reverse transcriptase affect the initiation of DNA synthesis and the specificity of RNase H cleavage in vivo. Proc Natl Acad Sci U S A (2002) 1.85
Reconstitution in vitro of RNase H activity by using purified N-terminal and C-terminal domains of human immunodeficiency virus type 1 reverse transcriptase. Proc Natl Acad Sci U S A (1991) 1.79
Processing of the primer for plus strand DNA synthesis by human immunodeficiency virus 1 reverse transcriptase. J Biol Chem (1990) 1.77
RNA-primed initiation of Moloney murine leukemia virus plus strands by reverse transcriptase in vitro. J Virol (1984) 1.70
Ribonuclease H activities associated with viral reverse transcriptases are endonucleases. Proc Natl Acad Sci U S A (1989) 1.67
Plus-strand priming by Moloney murine leukemia virus. The sequence features important for cleavage by RNase H. J Mol Biol (1989) 1.65
Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity. Biochemistry (2002) 1.64
Specificities involved in the initiation of retroviral plus-strand DNA. J Virol (1990) 1.55
Nuclease activities of Moloney murine leukemia virus reverse transcriptase. Mutants with altered substrate specificities. J Biol Chem (1993) 1.54
Effects of mutations in the polymerase domain on the polymerase, RNase H and strand transfer activities of human immunodeficiency virus type 1 reverse transcriptase. J Mol Biol (1998) 1.54
Sequence and structural determinants required for priming of plus-strand DNA synthesis by the human immunodeficiency virus type 1 polypurine tract. J Virol (1996) 1.53
Determinants of the RNase H cleavage specificity of human immunodeficiency virus reverse transcriptase. Nucleic Acids Res (1993) 1.46
'Binding, bending and bonding': polypurine tract-primed initiation of plus-strand DNA synthesis in human immunodeficiency virus. Int J Biochem Cell Biol (2004) 1.46
Crystal structure of Escherichia coli RNase HI in complex with Mg2+ at 2.8 A resolution: proof for a single Mg(2+)-binding site. Proteins (1993) 1.46
Specificity of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H in removal of the minus-strand primer, tRNA(Lys3). J Biol Chem (1992) 1.44
Incomplete removal of the RNA primer for minus-strand DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase. J Virol (1992) 1.43
Defects in Moloney murine leukemia virus replication caused by a reverse transcriptase mutation modeled on the structure of Escherichia coli RNase H. J Virol (1992) 1.41
The role of Moloney murine leukemia virus RNase H activity in the formation of plus-strand primers. J Virol (1987) 1.40
When retroviral reverse transcriptases reach the end of their RNA templates. J Virol (1992) 1.36
Human immunodeficiency virus reverse transcriptase displays a partially processive 3' to 5' endonuclease activity. J Biol Chem (1991) 1.35
Importance of the positive charge cluster in Escherichia coli ribonuclease HI for the effective binding of the substrate. J Biol Chem (1991) 1.34
Two defective forms of reverse transcriptase can complement to restore retroviral infectivity. EMBO J (1993) 1.33
Replication defect of moloney murine leukemia virus with a mutant reverse transcriptase that can incorporate ribonucleotides and deoxyribonucleotides. J Virol (1998) 1.33
Helix structure and ends of RNA/DNA hybrids direct the cleavage specificity of HIV-1 reverse transcriptase RNase H. J Biol Chem (1996) 1.32
Unique progressive cleavage mechanism of HIV reverse transcriptase RNase H. Proc Natl Acad Sci U S A (2000) 1.30
RNase H activity: structure, specificity, and function in reverse transcription. Virus Res (2008) 1.30
Mutation of amino acids in the connection domain of human immunodeficiency virus type 1 reverse transcriptase that contact the template-primer affects RNase H activity. J Virol (2003) 1.28
The sequence features important for plus strand priming by human immunodeficiency virus type 1 reverse transcriptase. J Biol Chem (1993) 1.26
RNase H cleavage of tRNAPro mediated by M-MuLV and HIV-1 reverse transcriptases. Virology (1997) 1.25
Effect of RNA secondary structure on the kinetics of DNA synthesis catalyzed by HIV-1 reverse transcriptase. Biochemistry (1997) 1.25
Interaction of retroviral reverse transcriptase with template-primer duplexes during replication. Prog Nucleic Acid Res Mol Biol (1998) 1.23
Contributions of DNA polymerase subdomains to the RNase H activity of human immunodeficiency virus type 1 reverse transcriptase. J Virol (1994) 1.21
Purification and characterization of an active human immunodeficiency virus type 1 RNase H domain. J Virol (1993) 1.19
Use of an oligoribonucleotide containing the polypurine tract sequence as a primer by HIV reverse transcriptase. J Biol Chem (1995) 1.17
The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus. Structure (2004) 1.13
Footprint analysis of replicating murine leukemia virus reverse transcriptase. Science (1995) 1.13
Characterization of RNA strand displacement synthesis by Moloney murine leukemia virus reverse transcriptase. J Biol Chem (1998) 1.13
Residues in the alphaH and alphaI helices of the HIV-1 reverse transcriptase thumb subdomain required for the specificity of RNase H-catalyzed removal of the polypurine tract primer. J Biol Chem (1999) 1.12
Mutations of the RNase H C helix of the Moloney murine leukemia virus reverse transcriptase reveal defects in polypurine tract recognition. J Virol (2002) 1.12
Mutational analysis of Tyr-501 of HIV-1 reverse transcriptase. Effects on ribonuclease H activity and inhibition of this activity by N-acylhydrazones. J Biol Chem (2001) 1.11
Characterization of the double stranded RNA dependent RNase activity associated with recombinant reverse transcriptases. Nucleic Acids Res (1992) 1.08
An expanded model of replicating human immunodeficiency virus reverse transcriptase. Biochemistry (1995) 1.08
The sequential mechanism of HIV reverse transcriptase RNase H. J Biol Chem (2000) 1.08
Strand displacement synthesis capability of Moloney murine leukemia virus reverse transcriptase. J Virol (1994) 1.06
Effects of mutations in the G tract of the human immunodeficiency virus type 1 polypurine tract on virus replication and RNase H cleavage. J Virol (2004) 1.06
Quantitative analysis of RNA cleavage during RNA-directed DNA synthesis by human immunodeficiency and avian myeloblastosis virus reverse transcriptases. Nucleic Acids Res (1994) 1.06
Selection of optimal polypurine tract region sequences during Moloney murine leukemia virus replication. J Virol (2000) 1.04
A recombinant ribonuclease H domain of HIV-1 reverse transcriptase that is enzymatically active. J Biol Chem (1991) 1.04
Crystal structure of the moloney murine leukemia virus RNase H domain. J Virol (2006) 1.04
Revealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteins. J Virol (2006) 1.03
The orientation of binding of human immunodeficiency virus reverse transcriptase on nucleic acid hybrids. Nucleic Acids Res (1995) 1.02
The isolated RNase H domain of murine leukemia virus reverse transcriptase. Retention of activity with concomitant loss of specificity. J Biol Chem (1997) 1.02
Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine-thymine tracts. Proc Natl Acad Sci U S A (2002) 1.01
RNase H domain of Moloney murine leukemia virus reverse transcriptase retains activity but requires the polymerase domain for specificity. J Virol (1996) 1.01
Redesignation of the RNase D activity associated with retroviral reverse transcriptase as RNase H. J Virol (1994) 0.99
Combining mutations in HIV-1 reverse transcriptase with mutations in the HIV-1 polypurine tract affects RNase H cleavages involved in PPT utilization. Virology (2006) 0.98
Polypurine tract primer generation and utilization by Moloney murine leukemia virus reverse transcriptase. J Biol Chem (1999) 0.98
Substrate requirements for secondary cleavage by HIV-1 reverse transcriptase RNase H. J Biol Chem (2002) 0.98
Effect of RNA secondary structure on RNA cleavage catalyzed by HIV-1 reverse transcriptase. Biochemistry (1997) 0.97
RNA degradation and primer selection by Moloney murine leukemia virus reverse transcriptase contribute to the accuracy of plus strand initiation. J Biol Chem (2000) 0.97
Sequence, distance, and accessibility are determinants of 5'-end-directed cleavages by retroviral RNases H. J Biol Chem (2005) 0.97
Analysis of plus-strand primer selection, removal, and reutilization by retroviral reverse transcriptases. J Biol Chem (2000) 0.96
The use of DNA and RNA oligonucleotides in hybrid structures with longer polynucleotide chains to probe the structural requirements for moloney murine leukemia virus plus strand priming. J Biol Chem (1994) 0.95
Recognition of internal cleavage sites by retroviral RNases H. J Mol Biol (2004) 0.92
In vitro analysis of human immunodeficiency virus type 1 minus-strand strong-stop DNA synthesis and genomic RNA processing. J Virol (2001) 0.92
Discontinuous plus-strand DNA synthesis in human immunodeficiency virus type 1-infected cells and in a partially reconstituted cell-free system. J Virol (1997) 0.92
RNase H activity of reverse transcriptases on substrates derived from the 5' end of retroviral genome. J Biol Chem (1993) 0.92
Purine analog substitution of the HIV-1 polypurine tract primer defines regions controlling initiation of plus-strand DNA synthesis. Nucleic Acids Res (2006) 0.89
Specific cleavages by RNase H facilitate initiation of plus-strand RNA synthesis by Moloney murine leukemia virus. J Virol (2003) 0.89
Cleavage specificities of Moloney murine leukemia virus RNase H implicated in the second strand transfer during reverse transcription. J Biol Chem (1995) 0.88
Structural alterations in the DNA ahead of the primer terminus during displacement synthesis by reverse transcriptases. J Mol Biol (2001) 0.87
Mutations in the RNase H primer grip domain of murine leukemia virus reverse transcriptase decrease efficiency and accuracy of plus-strand DNA transfer. J Virol (2005) 0.85
Analysis of HIV-1 replication block due to substitutions at F61 residue of reverse transcriptase reveals additional defects involving the RNase H function. Nucleic Acids Res (2006) 0.84
In vitro analysis of the effects of mutations in the G-tract of the human immunodeficiency virus type 1 polypurine tract on RNase H cleavage specificity. Virology (2006) 0.82
Sequence requirements for removal of tRNA by an isolated human immunodeficiency virus type 1 RNase H domain. J Virol (1998) 0.81
RNase H cleavage of the 5' end of the human immunodeficiency virus type 1 genome. J Virol (2001) 0.78
Coupling of reverse transcriptase and RNase H during HIV-1 replication. Behring Inst Mitt (1991) 0.76
DNA relaxation by human topoisomerase I occurs in the closed clamp conformation of the protein. Proc Natl Acad Sci U S A (2003) 0.98
Substitution of alanine for tyrosine-64 in the fingers subdomain of M-MuLV reverse transcriptase impairs strand displacement synthesis and blocks viral replication in vivo. Virology (2007) 0.80