Rapid real-time nucleic Acid sequence-based amplification-molecular beacon platform to detect fungal and bacterial bloodstream infections.

PubWeight™: 1.58‹?› | Rank: Top 4%

🔗 View Article (PMC 2708467)

Published in J Clin Microbiol on April 29, 2009

Authors

Yanan Zhao1, Steven Park, Barry N Kreiswirth, Christine C Ginocchio, Raphaël Veyret, Ali Laayoun, Alain Troesch, David S Perlin

Author Affiliations

1: Public Health Research Institute, UMDNJ-New Jersey Medical School, Newark, NJ 07103, USA.

Articles citing this

Rapid universal identification of bacterial pathogens from clinical cultures by using a novel sloppy molecular beacon melting temperature signature technique. J Clin Microbiol (2009) 1.44

Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia. J Clin Microbiol (2010) 1.38

Antifungal drug resistance: do molecular methods provide a way forward? Curr Opin Infect Dis (2009) 1.20

Detection of Aspergillus fumigatus in a rat model of invasive pulmonary aspergillosis by real-time nucleic acid sequence-based amplification. J Clin Microbiol (2010) 1.17

Fungal DNA detected in blood samples of patients who received contaminated methylprednisolone injections reveals increased complexity of causative agents. J Clin Microbiol (2014) 1.14

Use and evaluation of molecular diagnostics for pneumonia etiology studies. Clin Infect Dis (2012) 1.00

A MIQE-compliant real-time PCR assay for Aspergillus detection. PLoS One (2012) 0.99

Highly sensitive detection of Staphylococcus aureus directly from patient blood. PLoS One (2012) 0.98

Detection of multiple fungal species in blood samples by real-time PCR: an interpretative challenge. J Clin Microbiol (2014) 0.97

Polymerase chain reaction-based assays for the diagnosis of invasive fungal infections. Clin Infect Dis (2012) 0.93

Molecular detection of bacterial pathogens using microparticle enhanced double-stranded DNA probes. Anal Chem (2011) 0.89

Pathogen-specific DNA enrichment does not increase sensitivity of PCR for diagnosis of invasive aspergillosis in neutropenic patients. J Clin Microbiol (2010) 0.88

Molecular beacons in diagnostics. F1000 Med Rep (2012) 0.82

Rapid identification of medically important Candida isolates using high resolution melting analysis. PLoS One (2015) 0.81

Real-time nucleic acid sequence-based amplification assay for rapid detection and quantification of agr functionality in clinical Staphylococcus aureus isolates. J Clin Microbiol (2012) 0.79

Recent Advances in the Detection of Neonatal Candidiasis. Curr Fungal Infect Rep (2010) 0.78

Single quantum dot analysis enables multiplexed point mutation detection by gap ligase chain reaction. Small (2012) 0.78

Molecular Diagnostic Testing for Aspergillus. J Clin Microbiol (2016) 0.75

Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis. AMB Express (2016) 0.75

A novel cassette method for probe evaluation in the designed biochips. PLoS One (2014) 0.75

Reply to "detection of multiple fungal species in blood samples by real-time PCR: an interpretative challenge". J Clin Microbiol (2014) 0.75

Occurrence of Fungal DNA Contamination in PCR Reagents: Approaches to Control and Decontamination. J Clin Microbiol (2015) 0.75

Washed Away; How Not to Lose Your RNA during Isolation. J Biomol Tech (2017) 0.75

Articles cited by this

Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol (1996) 21.49

Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis (2004) 20.99

Invasive aspergillosis. Clin Infect Dis (1998) 9.72

The influence of inadequate antimicrobial treatment of bloodstream infections on patient outcomes in the ICU setting. Chest (2000) 9.51

Secular trends in nosocomial primary bloodstream infections in the United States, 1980-1989. National Nosocomial Infections Surveillance System. Am J Med (1991) 9.11

An overview of nosocomial infections, including the role of the microbiology laboratory. Clin Microbiol Rev (1993) 8.03

Nucleic acid sequence-based amplification. Nature (1991) 5.66

Delaying the empiric treatment of candida bloodstream infection until positive blood culture results are obtained: a potential risk factor for hospital mortality. Antimicrob Agents Chemother (2005) 5.35

Inappropriate initial antimicrobial therapy and its effect on survival in a clinical trial of immunomodulating therapy for severe sepsis. Am J Med (2003) 4.56

Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. J Clin Microbiol (2000) 4.44

Deaths: final data for 2004. Natl Vital Stat Rep (2007) 3.73

New developments in the diagnosis of bloodstream infections. Lancet Infect Dis (2004) 3.01

Development of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service. J Med Microbiol (2003) 2.84

Microbiological factors influencing the outcome of nosocomial bloodstream infections: a 6-year validated, population-based model. Clin Infect Dis (1997) 2.67

Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J Clin Microbiol (2003) 2.47

Etiologic diagnosis of infective endocarditis by broad-range polymerase chain reaction: a 3-year experience. Clin Infect Dis (2003) 2.23

Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens. J Clin Microbiol (2002) 2.12

Controlled clinical evaluation of Isolator and ESP aerobic blood culture systems for detection of bloodstream infections. J Clin Microbiol (1994) 2.07

Comparing diagnostic tests: a simple graphic using likelihood ratios. Stat Med (2000) 1.74

Molecular methods for the assessment of bacterial viability. J Microbiol Methods (2003) 1.68

Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion. J Clin Microbiol (1999) 1.63

Real-time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated for sepsis. J Mol Diagn (2005) 1.61

National Nosocomial Infections Surveillance (NNIS) system report, data summary from January 1992-April 2000, issued June 2000. Am J Infect Control (2000) 1.60

Adjustment of antibiotic treatment according to the results of blood cultures leads to decreased antibiotic use and costs. J Antimicrob Chemother (2005) 1.50

Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification. J Clin Microbiol (2006) 1.49

Controlled comparative evaluation of BacT/Alert FAN and ESP 80A aerobic media as means for detecting bacteremia and fungemia. J Clin Microbiol (1998) 1.40

Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens. J Clin Microbiol (2007) 1.37

Isolation and detection of enterovirus RNA from large-volume water samples by using the NucliSens miniMAG system and real-time nucleic acid sequence-based amplification. Appl Environ Microbiol (2005) 1.33

Severe sepsis: variation in resource and therapeutic modality use among academic centers. Crit Care (2003) 1.24

Evaluation of a protocol for molecular broad-range diagnosis of culture-negative bacterial infections in clinical routine diagnosis. J Appl Microbiol (2007) 1.22

Effects of delayed-entry conditions on the recovery and detection of microorganisms from BacT/ALERT and BACTEC blood culture bottles. J Clin Microbiol (2006) 1.13

The Pan-AC assay: a single-reaction real-time PCR test for quantitative detection of a broad range of Aspergillus and Candida species. J Med Microbiol (2007) 1.05

Nucleic acid sequence-based amplification of Aspergillus RNA in blood samples. J Clin Microbiol (2001) 1.03

Use of a panfungal PCR assay for detection of fungal pathogens in a commercial blood culture system. J Clin Microbiol (2004) 1.02

Controlled clinical comparison of VersaTREK and BacT/ALERT blood culture systems. J Clin Microbiol (2006) 1.01

An RNA transcription-based amplification technique (NASBA) for the detection of viable Salmonella enterica. Lett Appl Microbiol (2000) 0.98

Evaluation of a real-time nucleic acid sequence-based amplification assay using molecular beacons for detection of human immunodeficiency virus type 1. J Clin Microbiol (2006) 0.97

Real-time nucleic acid sequence-based amplification using molecular beacons for detection of enterovirus RNA in clinical specimens. J Clin Microbiol (2005) 0.95

Detection of Candida spp. in blood cultures using nucleic acid sequence-based amplification (NASBA). Diagn Microbiol Infect Dis (2001) 0.94

Nucleic acid sequence-based amplification (NASBA) detection of medically important Candida species. J Microbiol Methods (1999) 0.93

Evaluation of pan-fungal PCR assay and Aspergillus antigen detection in the diagnosis of invasive fungal infections in high risk paediatric cancer patients. Med Mycol (2006) 0.92

Detection of Vibrio cholerae by real-time nucleic acid sequence-based amplification. Appl Environ Microbiol (2007) 0.90

A molecular beacon-based real time NASBA assay for detection of Listeria monocytogenes in food products: role of target mRNA secondary structure on NASBA design. J Microbiol Methods (2007) 0.87

The mechanism for low yield of blood culture in invasive aspergillosis; the clinical importance of antigen detection tests revisited. Bone Marrow Transplant (2005) 0.87

Development of conventional and real-time NASBA for the detection of Legionella species in respiratory specimens. J Microbiol Methods (2006) 0.86

Real-time broad-range PCR versus blood culture. A prospective pilot study in pediatric cancer patients with fever and neutropenia. Support Care Cancer (2007) 0.86

Improving molecular detection of Candida DNA in whole blood: comparison of seven fungal DNA extraction protocols using real-time PCR. J Med Microbiol (2008) 0.85

Detection of Campylobacter jejuni and Campylobacter coli in chicken meat samples by real-time nucleic acid sequence-based amplification with molecular beacons. Int J Food Microbiol (2007) 0.83

Development and evaluation of the nuclisens basic kit NASBA for the detection of RNA from Candida species frequently resistant to antifungal drugs. Diagn Microbiol Infect Dis (2003) 0.80

Role of multiple site blood cultures to document the clearance of bacteremia in neonates. J Perinatol (2007) 0.78

Articles by these authors

Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol (2006) 11.86

Community-associated meticillin-resistant Staphylococcus aureus. Lancet (2010) 9.07

Global dissemination of the Mycobacterium tuberculosis W-Beijing family strains. Trends Microbiol (2002) 8.37

Is Panton-Valentine leukocidin the major virulence determinant in community-associated methicillin-resistant Staphylococcus aureus disease? J Infect Dis (2006) 8.17

Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother (2006) 7.03

A glycolipid of hypervirulent tuberculosis strains that inhibits the innate immune response. Nature (2004) 6.29

Insights into mechanisms used by Staphylococcus aureus to avoid destruction by human neutrophils. J Immunol (2005) 5.18

spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation. J Clin Microbiol (2004) 4.80

Frequency and evolution of Azole resistance in Aspergillus fumigatus associated with treatment failure. Emerg Infect Dis (2009) 4.71

Epidemic community-associated methicillin-resistant Staphylococcus aureus: recent clonal expansion and diversification. Proc Natl Acad Sci U S A (2008) 4.38

Better tests, better care: improved diagnostics for infectious diseases. Clin Infect Dis (2013) 4.27

Global distribution of Mycobacterium tuberculosis spoligotypes. Emerg Infect Dis (2002) 4.22

Snapshot of moving and expanding clones of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an international study. J Clin Microbiol (2003) 4.19

Increasing echinocandin resistance in Candida glabrata: clinical failure correlates with presence of FKS mutations and elevated minimum inhibitory concentrations. Clin Infect Dis (2013) 4.14

Genome-wide analysis of synonymous single nucleotide polymorphisms in Mycobacterium tuberculosis complex organisms: resolution of genetic relationships among closely related microbial strains. Genetics (2002) 4.11

Definition of the Beijing/W lineage of Mycobacterium tuberculosis on the basis of genetic markers. J Clin Microbiol (2004) 4.03

Tackling antibiotic resistance. Nat Rev Microbiol (2011) 3.95

Molecular epidemiology of tuberculosis: current insights. Clin Microbiol Rev (2006) 3.79

Genomic deletions classify the Beijing/W strains as a distinct genetic lineage of Mycobacterium tuberculosis. J Clin Microbiol (2005) 3.67

2009 H1N1 influenza infection in cancer patients and hematopoietic stem cell transplant recipients. J Infect (2010) 3.56

Single-nucleotide polymorphism-based population genetic analysis of Mycobacterium tuberculosis strains from 4 geographic sites. J Infect Dis (2005) 3.32

Progressive loss of echinocandin activity following prolonged use for treatment of Candida albicans oesophagitis. J Antimicrob Chemother (2006) 3.27

Effect of Candida glabrata FKS1 and FKS2 mutations on echinocandin sensitivity and kinetics of 1,3-beta-D-glucan synthase: implication for the existing susceptibility breakpoint. Antimicrob Agents Chemother (2009) 3.13

Correlating echinocandin MIC and kinetic inhibition of fks1 mutant glucan synthases for Candida albicans: implications for interpretive breakpoints. Antimicrob Agents Chemother (2008) 3.05

Mycobacterium tuberculosis growth at the cavity surface: a microenvironment with failed immunity. Infect Immun (2003) 3.01

Multiple resistance mechanisms among Aspergillus fumigatus mutants with high-level resistance to itraconazole. Antimicrob Agents Chemother (2003) 2.83

Contribution of Panton-Valentine leukocidin in community-associated methicillin-resistant Staphylococcus aureus pathogenesis. PLoS One (2008) 2.62

Molecular dissection of the evolution of carbapenem-resistant multilocus sequence type 258 Klebsiella pneumoniae. Proc Natl Acad Sci U S A (2014) 2.56

Breakthrough invasive candidiasis in patients on micafungin. J Clin Microbiol (2010) 2.47

A naturally occurring proline-to-alanine amino acid change in Fks1p in Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis accounts for reduced echinocandin susceptibility. Antimicrob Agents Chemother (2008) 2.44

Reduced Candida glabrata susceptibility secondary to an FKS1 mutation developed during candidemia treatment. Antimicrob Agents Chemother (2008) 2.41

Echinocandin susceptibility testing of Candida species: comparison of EUCAST EDef 7.1, CLSI M27-A3, Etest, disk diffusion, and agar dilution methods with RPMI and isosensitest media. Antimicrob Agents Chemother (2009) 2.41

The clonality of Staphylococcus aureus nasal carriage. J Infect Dis (2005) 2.39

Assessing resistance to the echinocandin antifungal drug caspofungin in Candida albicans by profiling mutations in FKS1. Antimicrob Agents Chemother (2006) 2.37

Multi-azole resistance in Aspergillus fumigatus. Int J Antimicrob Agents (2006) 2.30

Progressive esophagitis caused by Candida albicans with reduced susceptibility to caspofungin. Pharmacotherapy (2006) 2.30

Prevalence of agr dysfunction among colonizing Staphylococcus aureus strains. J Infect Dis (2008) 2.29

Community-acquired methicillin-resistant Staphylococcus aureus: an emerging pathogen. Infect Control Hosp Epidemiol (2003) 2.27

Acquired echinocandin resistance in a Candida krusei isolate due to modification of glucan synthase. Antimicrob Agents Chemother (2007) 2.20

Multiplex real-time PCR assay for detection and classification of Klebsiella pneumoniae carbapenemase gene (bla KPC) variants. J Clin Microbiol (2010) 2.18

Serum differentially alters the antifungal properties of echinocandin drugs. Antimicrob Agents Chemother (2007) 2.18

Fitness and virulence costs of Candida albicans FKS1 hot spot mutations associated with echinocandin resistance. J Infect Dis (2011) 2.17

Breakthrough Aspergillus fumigatus and Candida albicans double infection during caspofungin treatment: laboratory characteristics and implication for susceptibility testing. Antimicrob Agents Chemother (2008) 2.13

Role of cell culture for virus detection in the age of technology. Clin Microbiol Rev (2007) 2.12

Caspofungin-resistant Candida tropicalis strains causing breakthrough fungemia in patients at high risk for hematologic malignancies. Antimicrob Agents Chemother (2008) 2.06

Global epidemiology of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA). Curr Opin Microbiol (2012) 2.06

Potential associations between hematogenous complications and bacterial genotype in Staphylococcus aureus infection. J Infect Dis (2007) 2.00

Neutrophil microbicides induce a pathogen survival response in community-associated methicillin-resistant Staphylococcus aureus. J Immunol (2008) 1.97

Genomic analysis identifies targets of convergent positive selection in drug-resistant Mycobacterium tuberculosis. Nat Genet (2013) 1.93

Mobile genetic element-encoded cytolysin connects virulence to methicillin resistance in MRSA. PLoS Pathog (2009) 1.89

Enhanced expression of dltABCD is associated with the development of daptomycin nonsusceptibility in a clinical endocarditis isolate of Staphylococcus aureus. J Infect Dis (2009) 1.89

Echinocandin resistance: an emerging clinical problem? Curr Opin Infect Dis (2014) 1.86

Studies of the paradoxical effect of caspofungin at high drug concentrations. Diagn Microbiol Infect Dis (2005) 1.83

Genetic polymorphism in Mycobacterium tuberculosis isolates from patients with chronic multidrug-resistant tuberculosis. J Infect Dis (2004) 1.75

Role of rapid immunochromatographic antigen testing in diagnosis of influenza A virus 2009 H1N1 infection. J Clin Microbiol (2009) 1.74

Prevalence and characteristics of Staphylococcus aureus colonization among healthcare professionals in an urban teaching hospital. Infect Control Hosp Epidemiol (2010) 1.73

Global analysis of community-associated methicillin-resistant Staphylococcus aureus exoproteins reveals molecules produced in vitro and during infection. Cell Microbiol (2007) 1.72

Multiplex real-time PCR for rapid Staphylococcal cassette chromosome mec typing. J Clin Microbiol (2009) 1.72

High-frequency triazole resistance found In nonculturable Aspergillus fumigatus from lungs of patients with chronic fungal disease. Clin Infect Dis (2011) 1.72

Characterization of a new cytotoxin that contributes to Staphylococcus aureus pathogenesis. Mol Microbiol (2010) 1.71

Complete nucleotide sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains isolated in New Jersey. Antimicrob Agents Chemother (2012) 1.70

Molecular differentiation of historic phage-type 80/81 and contemporary epidemic Staphylococcus aureus. Proc Natl Acad Sci U S A (2011) 1.70

An outbreak of methicillin-resistant Staphylococcus aureus in a neonatal intensive care unit. Infect Control Hosp Epidemiol (2003) 1.68

Novel FKS mutations associated with echinocandin resistance in Candida species. Antimicrob Agents Chemother (2010) 1.68

Laboratory surge response to pandemic (H1N1) 2009 outbreak, New York City metropolitan area, USA. Emerg Infect Dis (2010) 1.67

Differential distribution and expression of Panton-Valentine leucocidin among community-acquired methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol (2005) 1.67

Correlation of acetate catabolism and growth yield in Staphylococcus aureus: implications for host-pathogen interactions. Infect Immun (2003) 1.66

Nosocomial transmission of extensively drug-resistant tuberculosis in a rural hospital in South Africa. J Infect Dis (2012) 1.65

Multicenter study evaluating the Vitek MS system for identification of medically important yeasts. J Clin Microbiol (2013) 1.64

Genotype prevalence and risk factors for severe clinical adenovirus infection, United States 2004-2006. Clin Infect Dis (2007) 1.63

Rapid neutrophil destruction following phagocytosis of Staphylococcus aureus. J Innate Immun (2010) 1.62

In vitro evolution of itraconazole resistance in Aspergillus fumigatus involves multiple mechanisms of resistance. Antimicrob Agents Chemother (2004) 1.62

Application of sensitive and specific molecular methods to uncover global dissemination of the major RDRio Sublineage of the Latin American-Mediterranean Mycobacterium tuberculosis spoligotype family. J Clin Microbiol (2008) 1.59

Complete nucleotide sequence of a blaKPC-harboring IncI2 plasmid and its dissemination in New Jersey and New York hospitals. Antimicrob Agents Chemother (2013) 1.59

Requirement for ergosterol in V-ATPase function underlies antifungal activity of azole drugs. PLoS Pathog (2010) 1.58

"Eczema coxsackium" and unusual cutaneous findings in an enterovirus outbreak. Pediatrics (2013) 1.57

Multicenter evaluation of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of Gram-positive aerobic bacteria. J Clin Microbiol (2013) 1.56

A selective, Bayesian approach to intraoperative PTH monitoring. Ann Surg (2010) 1.56

The phenolic glycolipid of Mycobacterium tuberculosis differentially modulates the early host cytokine response but does not in itself confer hypervirulence. Infect Immun (2008) 1.55

Comparison of dimethyl sulfoxide and water as solvents for echinocandin susceptibility testing by the EUCAST methodology. J Clin Microbiol (2012) 1.49

Impact of pneumococcal conjugate vaccine and the severity of winter influenza-like illnesses on invasive pneumococcal infections in children and adults. Pediatr Infect Dis J (2005) 1.48

Emergence of carbapenem-resistant Enterobacteriaceae as causes of bloodstream infections in patients with hematologic malignancies. Leuk Lymphoma (2012) 1.47

Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans. Antimicrob Agents Chemother (2008) 1.46

Lipoarabinomannan in urine during tuberculosis treatment: association with host and pathogen factors and mycobacteriuria. BMC Infect Dis (2012) 1.46

Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone. Antimicrob Agents Chemother (2012) 1.46

Clinical strains of Mycobacterium tuberculosis display a wide range of virulence in guinea pigs. Tuberculosis (Edinb) (2009) 1.45