Published in Methods on January 28, 2010
Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples. Appl Environ Microbiol (2012) 1.03
How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments. Biomol Detect Quantif (2015) 1.01
Shape based kinetic outlier detection in real-time PCR. BMC Bioinformatics (2010) 0.93
Clinical relevance of SKP2 alterations in metastatic melanoma. Pigment Cell Melanoma Res (2010) 0.92
Validation of kinetics similarity in qPCR. Nucleic Acids Res (2011) 0.90
Validation of the β-amy1 transcription profiling assay and selection of reference genes suited for a RT-qPCR assay in developing barley caryopsis. PLoS One (2012) 0.88
N-acetylcysteine improves antitumoural response of Interferon alpha by NF-kB downregulation in liver cancer cells. Comp Hepatol (2012) 0.81
Simulation of between repeat variability in real time PCR reactions. PLoS One (2012) 0.81
SPIDIA-RNA: second external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses. PLoS One (2014) 0.79
Accurate and precise DNA quantification in the presence of different amplification efficiencies using an improved Cy0 method. PLoS One (2013) 0.78
Analysis of TaqMan Array Cards Data by an Assumption-Free Improvement of the maxRatio Algorithm Is More Accurate than the Cycle-Threshold Method. PLoS One (2016) 0.75
Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report. Data Brief (2016) 0.75
Practical data handling pipeline improves performance of qPCR-based circulating miRNA measurements. RNA (2017) 0.75
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem (2009) 40.90
RNA integrity and the effect on the real-time qRT-PCR performance. Mol Aspects Med (2006) 7.37
Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels. Genome Res (2005) 4.98
Standardized determination of real-time PCR efficiency from a single reaction set-up. Nucleic Acids Res (2003) 4.46
Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR. Biotechnol Lett (2006) 3.53
Properties of the reverse transcription reaction in mRNA quantification. Clin Chem (2004) 3.49
The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments. Clin Chem (2013) 3.38
Kinetic Outlier Detection (KOD) in real-time PCR. Nucleic Acids Res (2003) 3.17
Concurrent versus sequential chemoradiotherapy with cisplatin and vinorelbine in locally advanced non-small cell lung cancer: a randomized study. Lung Cancer (2004) 2.38
Protective role of reactive astrocytes in brain ischemia. J Cereb Blood Flow Metab (2007) 2.35
RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization. BMC Genomics (2011) 2.14
Comparison of reverse transcriptases in gene expression analysis. Clin Chem (2004) 1.93
Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications. Methods (2012) 1.90
Quantitative real-time PCR method for detection of B-lymphocyte monoclonality by comparison of kappa and lambda immunoglobulin light chain expression. Clin Chem (2003) 1.85
Normalization strategies for microRNA profiling experiments: a 'normal' way to a hidden layer of complexity? Biotechnol Lett (2010) 1.84
Inhibition of real-time RT-PCR quantification due to tissue-specific contaminants. Mol Cell Probes (2004) 1.64
Tissue of origin determines cancer-associated CpG island promoter hypermethylation patterns. Genome Biol (2012) 1.63
The need for transparency and good practices in the qPCR literature. Nat Methods (2013) 1.58
Is it possible to avoid hypopituitarism after irradiation of pituitary adenomas by the Leksell gamma knife? Eur J Endocrinol (2010) 1.52
Development of a highly automated and multiplexed targeted proteome pipeline and assay for 112 rat brain synaptic proteins. Proteomics (2015) 1.46
Defining cell populations with single-cell gene expression profiling: correlations and identification of astrocyte subpopulations. Nucleic Acids Res (2010) 1.37
Primer sequence disclosure: a clarification of the MIQE guidelines. Clin Chem (2011) 1.32
Design and optimization of reverse-transcription quantitative PCR experiments. Clin Chem (2009) 1.28
A new minor groove binding asymmetric cyanine reporter dye for real-time PCR. Nucleic Acids Res (2003) 1.21
Quantitative real-time PCR for cancer detection: the lymphoma case. Expert Rev Mol Diagn (2005) 1.18
The ongoing evolution of qPCR. Methods (2010) 1.12
Monitoring differentiation of human embryonic stem cells using real-time PCR. Stem Cells (2005) 1.11
Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte. Nucleic Acids Res (2007) 1.11
Tissue-specific expression pattern of bovine prion gene: quantification using real-time RT-PCR. Mol Cell Probes (2003) 1.10
RT-qPCR work-flow for single-cell data analysis. Methods (2012) 1.10
Gene expression profiling--Clusters of possibilities. Methods (2010) 1.08
Validation of extraction methods for total RNA and miRNA from bovine blood prior to quantitative gene expression analyses. Biotechnol Lett (2009) 1.05
Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices. Plant Mol Biol (2010) 1.04
Correction of RT-qPCR data for genomic DNA-derived signals with ValidPrime. Nucleic Acids Res (2012) 1.03
Single-cell gene-expression profiling and its potential diagnostic applications. Expert Rev Mol Diagn (2011) 1.01
Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli. BMC Genomics (2008) 1.00
Transcriptional biomarkers--high throughput screening, quantitative verification, and bioinformatical validation methods. Methods (2012) 0.99
Heterogeneity of astrocytes: from development to injury - single cell gene expression. PLoS One (2013) 0.97
Direct cell lysis for single-cell gene expression profiling. Front Oncol (2013) 0.97
Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography. Methods (2010) 0.96
Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis. AIDS Res Hum Retroviruses (2009) 0.96
Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs--a comparative study. PLoS One (2012) 0.96
Inflammation in the wall of abdominal aortic aneurysm and its role in the symptomatology of aneurysm. Cytokines Cell Mol Ther (2002) 0.95
Statistical aspects of quantitative real-time PCR experiment design. Methods (2010) 0.95
Differential gonadotropin-releasing hormone (GnRH) and GnRH receptor messenger ribonucleic acid expression patterns in different tissues of the female rat across the estrous cycle. Endocrinology (2005) 0.95
Prognostic significance of TIMP-1 in non-small cell lung cancer. Anticancer Res (2011) 0.93
Development and evaluation of three real-time immuno-PCR assemblages for quantification of PSA. J Immunol Methods (2005) 0.93
Direct integration of intensity-level data from Affymetrix and Illumina microarrays improves statistical power for robust reanalysis. BMC Med Genomics (2012) 0.93
The workflow of single-cell expression profiling using quantitative real-time PCR. Expert Rev Mol Diagn (2014) 0.92
Functional genomic and proteomic analysis reveals disruption of myelin-related genes and translation in a mouse model of early life neglect. Front Psychiatry (2011) 0.90
Validation of kinetics similarity in qPCR. Nucleic Acids Res (2011) 0.90
Combining sequence-specific probes and DNA binding dyes in real-time PCR for specific nucleic acid quantification and melting curve analysis. Biotechniques (2006) 0.89
Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing. Biotechnol Lett (2015) 0.89
Potential stemness of frozen-thawed testicular biopsies without sperm in infertile men included into the in vitro fertilization programme. J Biomed Biotechnol (2012) 0.89
Distinct expression/function of potassium and chloride channels contributes to the diverse volume regulation in cortical astrocytes of GFAP/EGFP mice. PLoS One (2012) 0.89
Expression of pluripotency and oocyte-related genes in single putative stem cells from human adult ovarian surface epithelium cultured in vitro in the presence of follicular fluid. Biomed Res Int (2013) 0.88
Single blastomere expression profiling of Xenopus laevis embryos of 8 to 32-cells reveals developmental asymmetry. Sci Rep (2013) 0.88
Analysis of in vitro and in vivo characteristics of human embryonic stem cell-derived neural precursors. Cell Transplant (2009) 0.87
Gamma knife radiosurgery for acromegaly--long-term experience. Clin Endocrinol (Oxf) (2006) 0.87
Differential expression of molecular motors in the motor cortex of sporadic ALS. Neurobiol Dis (2007) 0.86
Changes in the miRNA profile under the influence of anabolic steroids in bovine liver. Analyst (2011) 0.86
Properties of targeted preamplification in DNA and cDNA quantification. Expert Rev Mol Diagn (2015) 0.86
A method to assess 59Fe in residual tissue blood content in mice and its use to correct 59Fe-distribution kinetics accordingly. Toxicology (2007) 0.86
Breast cancer risk assessment in the Czech female population--an adjustment of the original Gail model. Breast Cancer Res Treat (2005) 0.86
mRNA and microRNA purity and integrity: the key to success in expression profiling. Methods Mol Biol (2014) 0.85
Expression of estrogen and progesterone receptors in the bovine ovary during estrous cycle and pregnancy. Endocrine (2002) 0.85
Electric cell-substrate impedance sensing (ECIS) based real-time measurement of titer dependent cytotoxicity induced by adenoviral vectors in an IPI-2I cell culture model. Biosens Bioelectron (2010) 0.85
Differential global gene expression in cystic fibrosis nasal and bronchial epithelium. Genomics (2011) 0.85
Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers. Anal Chim Acta (2006) 0.85
Neural cell adhesion molecule-deficient beta-cell tumorigenesis results in diminished extracellular matrix molecule expression and tumour cell-matrix adhesion. Tumour Biol (2005) 0.83
Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis. Clin Chim Acta (2012) 0.83
Maternal separation with early weaning: a rodent model providing novel insights into neglect associated developmental deficits. Dev Psychopathol (2012) 0.83
RNA-sequencing as useful screening tool in the combat against the misuse of anabolic agents. Anal Chem (2012) 0.82
Parallel declines in cognition, motivation, and locomotion in aging mice: association with immune gene upregulation in the medial prefrontal cortex. Exp Gerontol (2011) 0.82
Use of the Leksell gamma knife in the treatment of prolactinoma patients. Clin Endocrinol (Oxf) (2008) 0.82
Effects of mycophenolic acid (MPA) treatment on expression of Fc receptor (FcRn) and polymeric immunoglobulin receptor (pIgR) mRNA in adult sheep tissues. Croat Med J (2004) 0.82
KIT receptor is expressed in more than 50% of early-stage malignant melanoma: a retrospective study of 261 patients. Melanoma Res (2005) 0.81
Identification of potential gene expression biomarkers for the surveillance of anabolic agents in bovine blood cells. Anal Chim Acta (2009) 0.81
The added value of single-cell gene expression profiling. Brief Funct Genomics (2013) 0.81
Plasma TIMP1 level is a prognostic factor in patients with liver metastases. Anticancer Res (2012) 0.81
Parameters of biological activity in colorectal cancer. Anticancer Res (2011) 0.81
Spectrophotometric and thermodynamic study on the dimerization equilibrium of ionic dyes in water by chemometrics method. Spectrochim Acta A Mol Biomol Spectrosc (2006) 0.80
Identification of a potential gene expression biomarker signature in bovine liver to detect the abuse of growth promoters. Food Addit Contam Part A Chem Anal Control Expo Risk Assess (2014) 0.80
Vitamin D in colorectal, breast, prostate and lung cancer: a pilot study. Anticancer Res (2011) 0.80
Transcriptional biomarkers. Methods (2013) 0.80
Posttranscriptional regulatory networks: from expression profiling to integrative analysis of mRNA and microRNA data. Methods Mol Biol (2014) 0.80
The use of omic technologies for biomarker development to trace functions of anabolic agents. J Chromatogr A (2009) 0.80
Detection of immune cell response to M. tuberculosis-specific antigens by quantitative polymerase chain reaction. Diagn Microbiol Infect Dis (2011) 0.80
Expression of immune relevant genes in pigs under the influence of low doses of deoxynivalenol (DON). Mycotoxin Res (2011) 0.80
Association of polymorphisms in HCN4 with mood disorders and obsessive compulsive disorder. Neurosci Lett (2011) 0.80
Quantitative mRNA analysis of eight bovine 5-HT receptor subtypes in brain, abomasum, and intestine by real-time RT-PCR. J Recept Signal Transduct Res (2003) 0.79
Influence of testosterone and a novel SARM on gene expression in whole blood of Macaca fascicularis. J Steroid Biochem Mol Biol (2009) 0.78
The analysis of the transcriptome as a new approach for biomarker development to trace the abuse of anabolic steroid hormones. Drug Test Anal (2011) 0.78
Increased expression of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in reactive astrocytes following ischemia. Glia (2014) 0.78
I see the light! And I see it again and again! Clin Chem (2012) 0.78