Quantification of fluorescence intensity of labeled human mesenchymal stem cells and cell counting of unlabeled cells in phase-contrast imaging: an open-source-based algorithm.

PubWeight™: 0.80‹?›

🔗 View Article (PMID 20218817)

Published in Tissue Eng Part C Methods on April 14, 2010

Authors

Hans Polzer1, Florian Haasters, Wolf Christian Prall, Maximilian Michael Saller, Elias Volkmer, Inga Drosse, Wolf Mutschler, Matthias Schieker

Author Affiliations

1: Department of Surgery, Experimental Surgery and Regenerative Medicine, University of Munich (LMU), Munich, Germany.

Articles by these authors

Effect of whole-body CT during trauma resuscitation on survival: a retrospective, multicentre study. Lancet (2009) 7.14

Serum S-100B and interleukin-8 as predictive markers for comparative neurologic outcome analysis of patients after cardiac arrest and severe traumatic brain injury. Crit Care Med (2002) 2.42

Conversion of human bone marrow-derived mesenchymal stem cells into tendon progenitor cells by ectopic expression of scleraxis. Stem Cells Dev (2011) 1.91

Human mesenchymal stem cells in contact with their environment: surface characteristics and the integrin system. J Cell Mol Med (2007) 1.69

Hypoxia in static and dynamic 3D culture systems for tissue engineering of bone. Tissue Eng Part A (2008) 1.65

Immediate S-100B and neuron-specific enolase plasma measurements for rapid evaluation of primary brain damage in alcohol-intoxicated, minor head-injured patients. Shock (2002) 1.55

Susceptibility of automated external defibrillators to train overhead lines and metro third rails. Resuscitation (2004) 1.54

Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res (2005) 1.53

Increased activation of the transcription factor c-Jun by MAP kinases in monocytes of multiple trauma patients is associated with adverse outcome and mass transfusion. J Surg Res (2012) 1.43

Researching into the cellular shape, volume and elasticity of mesenchymal stem cells, osteoblasts and osteosarcoma cells by atomic force microscopy. J Cell Mol Med (2008) 1.40

Serum S-100B concentration provides additional information fot the indication of computed tomography in patients after minor head injury: a prospective multicenter study. Shock (2006) 1.37

Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer. J Cell Mol Med (2008) 1.31

Pneumopericardium in blunt chest trauma after high-speed motor vehicle accidents. Am J Emerg Med (2005) 1.30

Hydroxyapatite scaffolds for bone tissue engineering made by 3D printing. J Mater Sci Mater Med (2005) 1.30

Osteoporosis and bisphosphonates-related osteonecrosis of the jaw: not just a sporadic coincidence--a multi-centre study. J Craniomaxillofac Surg (2010) 1.28

Morphological and immunocytochemical characteristics indicate the yield of early progenitors and represent a quality control for human mesenchymal stem cell culturing. J Anat (2009) 1.24

Emergency chest tube placement in trauma care - which approach is preferable? Resuscitation (2006) 1.23

Tissue engineering for bone defect healing: an update on a multi-component approach. Injury (2008) 1.22

Outcome in 757 severely injured patients with traumatic cardiorespiratory arrest. Resuscitation (2007) 1.19

Polychrome labeling of bone with seven different fluorochromes: enhancing fluorochrome discrimination by spectral image analysis. Bone (2005) 1.14

Human mesenchymal stem cells at the single-cell level: simultaneous seven-colour immunofluorescence. J Anat (2007) 1.10

Flexible intramedullary nailing for stabilization of displaced midshaft clavicle fractures: technique and results in 87 patients. Acta Orthop (2007) 1.08

The use of four-colour immunofluorescence techniques to identify mesenchymal stem cells. J Anat (2004) 1.07

[Letter to M. A. Scherer: ruptures of extensor tendons as a complication of internal plate fixation of distal radius fractures]. Versicherungsmedizin (2014) 1.06

IKK-2 is required for TNF-alpha-induced invasion and proliferation of human mesenchymal stem cells. J Mol Med (Berl) (2008) 1.06

Uncovering the cellular and molecular changes in tendon stem/progenitor cells attributed to tendon aging and degeneration. Aging Cell (2013) 1.05

Elevated expression of carcinoembryonic antigen-related cell adhesion molecule 1 promotes progression of non-small cell lung cancer. Clin Cancer Res (2003) 1.05

Trauma management incorporating focused assessment with computed tomography in trauma (FACTT) - potential effect on survival. J Trauma Manag Outcomes (2010) 1.04

Dexamethasone modulates BMP-2 effects on mesenchymal stem cells in vitro. J Orthop Res (2008) 1.04

Three-dimensional CT with a modified C-arm image intensifier: feasibility. Radiology (2002) 1.03

Tumour cells in the tumour draining vein of patients with non-small cell lung cancer: detection rate and clinical significance. Eur J Cardiothorac Surg (2003) 1.03

Osteonecrosis of the jaw: effect of bisphosphonate type, local concentration, and acidic milieu on the pathomechanism. J Oral Maxillofac Surg (2010) 1.02

Hypoxic preconditioning of human mesenchymal stem cells overcomes hypoxia-induced inhibition of osteogenic differentiation. Tissue Eng Part A (2010) 1.01

Nonviral genetic modification mediates effective transgene expression and functional RNA interference in human mesenchymal stem cells. J Gene Med (2005) 1.01

Prognostic impact of matrix metalloproteinase-9 in operable non-small cell lung cancer. Int J Cancer (2003) 0.99

Prepatellar and olecranon bursitis: literature review and development of a treatment algorithm. Arch Orthop Trauma Surg (2013) 0.99

Quantitative polymerase chain reaction as a reliable method to determine functional lentiviral titer after ex vivo gene transfer in human mesenchymal stem cells. J Gene Med (2007) 0.99

Effect of collagen I and fibronectin on the adhesion, elasticity and cytoskeletal organization of prostate cancer cells. Biochem Biophys Res Commun (2010) 0.99

Consistency of long-term outcome of acute Rockwood grade III acromioclavicular joint separations after K-wire transfixation. J Trauma (2009) 0.99

Genome-wide monocytic mRNA expression in polytrauma patients for identification of clinical outcome. Shock (2005) 0.97

Increased stemness and migration of human mesenchymal stem cells in hypoxia is associated with altered integrin expression. Biochem Biophys Res Commun (2012) 0.97

First inducible transgene expression in porcine large animal models. FASEB J (2011) 0.96

Validation of a femoral critical size defect model for orthotopic evaluation of bone healing: a biomechanical, veterinary and trauma surgical perspective. Tissue Eng Part C Methods (2008) 0.96

Impact of indium-111 oxine labelling on viability of human mesenchymal stem cells in vitro, and 3D cell-tracking using SPECT/CT in vivo. Mol Imaging Biol (2011) 0.95

Monoaxial versus polyaxial screw insertion in angular stable plate fixation of proximal humeral fractures: radiographic analysis of a prospective randomized study. J Trauma (2010) 0.95

Intrathecal and systemic concentration of NT-proBNP in patients with severe traumatic brain injury. J Neurotrauma (2006) 0.94

Differentiation of individual human mesenchymal stem cells probed by FTIR microscopic imaging. Analyst (2007) 0.92

New advances in fluorochrome sequential labelling of teeth using seven different fluorochromes and spectral image analysis. J Anat (2007) 0.91

Bisphosphonate-related osteonecrosis of the jaw: is pH the missing part in the pathogenesis puzzle? J Oral Maxillofac Surg (2010) 0.91

Diagnostic value of the Glasgow Coma Scale for traumatic brain injury in 18,002 patients with severe multiple injuries. J Neurotrauma (2011) 0.91

Celiac dissection after blunt abdominal trauma complicated by acute hepatic failure: case report and review of literature. J Vasc Surg (2007) 0.90

Mid-term outcome comparing temporary K-wire fixation versus PDS augmentation of Rockwood grade III acromioclavicular joint separations. BMC Res Notes (2009) 0.89

The bioexpandable prosthesis: a new perspective after resection of malignant bone tumors in children. J Pediatr Hematol Oncol (2005) 0.88

Diagnosis and treatment of acute ankle injuries: development of an evidence-based algorithm. Orthop Rev (Pavia) (2011) 0.88

C-arm-based three-dimensional navigation: a preliminary feasibility study. Comput Aided Surg (2003) 0.88

Characterisation of a new bioadhesive system based on polysaccharides with the potential to be used as bone glue. J Mater Sci Mater Med (2009) 0.88

Is the intraosseous access route fast and efficacious compared to conventional central venous catheterization in adult patients under resuscitation in the emergency department? A prospective observational pilot study. Patient Saf Surg (2009) 0.87

Effects of different media on proliferation and differentiation capacity of canine, equine and porcine adipose derived stem cells. Res Vet Sci (2011) 0.87

Mesenchymal stem cells from osteoporotic patients feature impaired signal transduction but sustained osteoinduction in response to BMP-2 stimulation. Biochem Biophys Res Commun (2013) 0.87