Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo.

PubWeight™: 1.26‹?› | Rank: Top 10%

🔗 View Article (PMID 20224175)

Published in Exp Anim on January 01, 2010

Authors

Hidetoshi Hasuwa1, Yuko Muro, Masahito Ikawa, Noriko Kato, Yoshihide Tsujimoto, Masaru Okabe

Author Affiliations

1: Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University.

Articles citing this

Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization. Proc Natl Acad Sci U S A (2011) 2.02

Fertilization: a sperm's journey to and interaction with the oocyte. J Clin Invest (2010) 1.53

Rheotaxis guides mammalian sperm. Curr Biol (2013) 1.47

Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility [corrected]. Proc Natl Acad Sci U S A (2012) 1.23

Unique insights into maternal mitochondrial inheritance in mice. Proc Natl Acad Sci U S A (2013) 1.12

Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa. Proc Natl Acad Sci U S A (2013) 1.00

Structurally distinct Ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility. Cell (2014) 0.98

Seminal vesicle protein SVS2 is required for sperm survival in the uterus. Proc Natl Acad Sci U S A (2014) 0.97

A comprehensive system for generation and evaluation of induced pluripotent stem cells using piggyBac transposition. PLoS One (2014) 0.87

Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization. Nat Commun (2016) 0.84

Testosterone replacement ameliorates nonalcoholic fatty liver disease in castrated male rats. Endocrinology (2013) 0.83

Redistribution of the intra-acrosomal EGFP before acrosomal exocytosis in mouse spermatozoa. Reproduction (2015) 0.80

Mouse sperm begin to undergo acrosomal exocytosis in the upper isthmus of the oviduct. Dev Biol (2016) 0.80

CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization. J Cell Biol (2015) 0.80

PKA-dependent phosphorylation of LIMK1 and Cofilin is essential for mouse sperm acrosomal exocytosis. Dev Biol (2015) 0.79

Mechanism of fertilization: a modern view. Exp Anim (2014) 0.79

ATG3-dependent autophagy mediates mitochondrial homeostasis in pluripotency acquirement and maintenance. Autophagy (2016) 0.79

Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells. Elife (2017) 0.78

Targeted disruption of Nrg1 in granulosa cells alters the temporal progression of oocyte maturation. Mol Endocrinol (2014) 0.78

Acrosome reaction and Ca²⁺ imaging in single human spermatozoa: new regulatory roles of [Ca²⁺]i. Biol Reprod (2014) 0.78

Src Kinase Is the Connecting Player between Protein Kinase A (PKA) Activation and Hyperpolarization through SLO3 Potassium Channel Regulation in Mouse Sperm. J Biol Chem (2015) 0.77

Improvement in Mouse iPSC Induction by Rab32 Reveals the Importance of Lipid Metabolism during Reprogramming. Sci Rep (2015) 0.76

Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis. Sci Adv (2017) 0.75

Mitochondrial fusion protein MFN2 interacts with the mitostatin-related protein MNS1 required for mouse sperm flagellar structure and function. Cilia (2014) 0.75

Current and future prospects for nanotechnology in animal production. J Anim Sci Biotechnol (2017) 0.75

Roles of the reproductive tract in modifications of the sperm membrane surface. J Reprod Dev (2016) 0.75

An intact acrosome is required for the chemotactic response to progesterone in mouse spermatozoa. Mol Reprod Dev (2017) 0.75

A Specific Transitory Increase in Intracellular Calcium Induced by Progesterone Promotes Acrosomal Exocytosis in Mouse Sperm. Biol Reprod (2016) 0.75

Embryo development after mitochondrial supplementation from induced pluripotent stem cells. J Assist Reprod Genet (2017) 0.75

Articles by these authors

Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway. Science (2003) 17.57

Role of Bcl-2 family proteins in a non-apoptotic programmed cell death dependent on autophagy genes. Nat Cell Biol (2004) 8.85

Cyclophilin D-dependent mitochondrial permeability transition regulates some necrotic but not apoptotic cell death. Nature (2005) 8.51

Discovery of Atg5/Atg7-independent alternative macroautophagy. Nature (2009) 6.74

DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. Genes Dev (2008) 5.58

Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development (2004) 5.47

Innate versus learned odour processing in the mouse olfactory bulb. Nature (2007) 4.19

A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc Natl Acad Sci U S A (2003) 4.17

The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature (2005) 4.07

Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization. Nat Med (2005) 4.02

Involvement of caspase-4 in endoplasmic reticulum stress-induced apoptosis and Abeta-induced cell death. J Cell Biol (2004) 3.85

PGC7/Stella protects against DNA demethylation in early embryogenesis. Nat Cell Biol (2006) 3.54

Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci U S A (2002) 3.53

A histone H3 lysine 36 trimethyltransferase links Nkx2-5 to Wolf-Hirschhorn syndrome. Nature (2009) 2.74

Plexin-A1 and its interaction with DAP12 in immune responses and bone homeostasis. Nat Cell Biol (2006) 2.57

JNK promotes Bax translocation to mitochondria through phosphorylation of 14-3-3 proteins. EMBO J (2004) 2.50

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci Rep (2013) 2.41

Fzo1, a protein involved in mitochondrial fusion, inhibits apoptosis. J Biol Chem (2004) 2.39

Signalling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation. Nat Cell Biol (2009) 2.38

Involvement of histone H1.2 in apoptosis induced by DNA double-strand breaks. Cell (2003) 2.38

Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization. Proc Natl Acad Sci U S A (2011) 2.02

Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice. Proc Natl Acad Sci U S A (2005) 1.98

Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer. Nat Biotechnol (2007) 1.94

14-3-3 Interacts directly with and negatively regulates pro-apoptotic Bax. J Biol Chem (2002) 1.84

Treatment of spinal cord injury by transplantation of fetal neural precursor cells engineered to express BMP inhibitor. Exp Neurol (2004) 1.82

Lats2 is an essential mitotic regulator required for the coordination of cell division. J Biol Chem (2007) 1.75

Distinct roles of IkappaB proteins in regulating constitutive NF-kappaB activity. Nat Cell Biol (2005) 1.75

Small interfering RNA and gene silencing in transgenic mice and rats. FEBS Lett (2002) 1.71

Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis. Nat Cell Biol (2009) 1.69

FISH analysis of 142 EGFP transgene integration sites into the mouse genome. Genomics (2002) 1.66

Neuroaxonal dystrophy caused by group VIA phospholipase A2 deficiency in mice: a model of human neurodegenerative disease. J Neurosci (2008) 1.64

Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the X-linked gene, Rhox5/Pem, at preimplantation stages. Curr Biol (2006) 1.61

Progressive adipocyte hypertrophy in aquaporin-7-deficient mice: adipocyte glycerol permeability as a novel regulator of fat accumulation. J Biol Chem (2005) 1.60

Pluripotency of a single spermatogonial stem cell in mice. Biol Reprod (2008) 1.59

Urinary excretion of fatty acid-binding protein reflects stress overload on the proximal tubules. Am J Pathol (2004) 1.56

Pravastatin induces placental growth factor (PGF) and ameliorates preeclampsia in a mouse model. Proc Natl Acad Sci U S A (2010) 1.56

Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg. J Biol Chem (2002) 1.53

Fertilization: a sperm's journey to and interaction with the oocyte. J Clin Invest (2010) 1.53

The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection. Cell Host Microbe (2010) 1.52

Nuclear translocation of caspase-3 is dependent on its proteolytic activation and recognition of a substrate-like protein(s). J Biol Chem (2004) 1.50

GPHR-dependent functions of the Golgi apparatus are essential for the formation of lamellar granules and the skin barrier. J Invest Dermatol (2012) 1.48

Neuromedin U has a novel anorexigenic effect independent of the leptin signaling pathway. Nat Med (2004) 1.48

Impaired urea accumulation in the inner medulla of mice lacking the urea transporter UT-A2. Mol Cell Biol (2005) 1.47

Delayed-onset caspase-dependent massive hepatocyte apoptosis upon Fas activation in Bak/Bax-deficient mice. Hepatology (2011) 1.47

Mitochondrial transcription factors TFAM and TFB2M regulate Serca2 gene transcription. Cardiovasc Res (2010) 1.47

Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells. Proc Natl Acad Sci U S A (2006) 1.45

Antitumor NK activation induced by the Toll-like receptor 3-TICAM-1 (TRIF) pathway in myeloid dendritic cells. Proc Natl Acad Sci U S A (2006) 1.42

Peroxiredoxin 4 knockout results in elevated spermatogenic cell death via oxidative stress. Biochem J (2009) 1.41

Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development (2013) 1.40

Nonredundant roles of Sema4A in the immune system: defective T cell priming and Th1/Th2 regulation in Sema4A-deficient mice. Immunity (2005) 1.39

Lysophosphatidylcholine as a death effector in the lipoapoptosis of hepatocytes. J Lipid Res (2007) 1.37

Functional characterization of a mouse testicular olfactory receptor and its role in chemosensing and in regulation of sperm motility. J Cell Sci (2004) 1.36

The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons. Eur J Neurosci (2004) 1.34

Cardiac-specific activation of signal transducer and activator of transcription 3 promotes vascular formation in the heart. J Biol Chem (2001) 1.33

Macrophage response to peripheral nerve injury: the quantitative contribution of resident and hematogenous macrophages. Lab Invest (2003) 1.33

IkappaB kinase-independent IkappaBalpha degradation pathway: functional NF-kappaB activity and implications for cancer therapy. Mol Cell Biol (2003) 1.29

Amniotic fluid and bone marrow derived mesenchymal stem cells can be converted to smooth muscle cells in the cryo-injured rat bladder and prevent compensatory hypertrophy of surviving smooth muscle cells. J Urol (2007) 1.28

Alteration of gene expression by chromosome loss in the postnatal mouse brain. J Neurosci (2003) 1.28

miR-200b and miR-429 function in mouse ovulation and are essential for female fertility. Science (2013) 1.27

Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility [corrected]. Proc Natl Acad Sci U S A (2012) 1.23

Disruption of mouse CD46 causes an accelerated spontaneous acrosome reaction in sperm. Mol Cell Biol (2003) 1.23

Evolutional study on acetylcholine expression. Life Sci (2003) 1.22

Dynamic modification of sphingomyelin in lipid microdomains controls development of obesity, fatty liver, and type 2 diabetes. J Biol Chem (2011) 1.22

Fetal microchimerism in the maternal mouse brain: a novel population of fetal progenitor or stem cells able to cross the blood-brain barrier? Stem Cells (2005) 1.22

BH4-domain peptide from Bcl-xL exerts anti-apoptotic activity in vivo. Oncogene (2003) 1.19

Listeria monocytogenes-infected bone marrow myeloid cells promote bacterial invasion of the central nervous system. Cell Microbiol (2005) 1.19

E-cadherin-coated plates maintain pluripotent ES cells without colony formation. PLoS One (2006) 1.17

Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice. J Biol Chem (2010) 1.16

Aberrant distribution of ADAM3 in sperm from both angiotensin-converting enzyme (Ace)- and calmegin (Clgn)-deficient mice. Biol Reprod (2006) 1.16

Hypertension and dysregulated proinflammatory cytokine production in receptor activity-modifying protein 1-deficient mice. Proc Natl Acad Sci U S A (2007) 1.15