1
|
Inclusion bodies: formation and utilisation.
|
Adv Biochem Eng Biotechnol
|
2004
|
1.46
|
2
|
A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures.
|
Microb Cell Fact
|
2010
|
1.44
|
3
|
Isolation and characterization of a T7-like lytic phage for Pseudomonas fluorescens.
|
BMC Biotechnol
|
2008
|
1.38
|
4
|
Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks.
|
Microb Cell Fact
|
2008
|
1.36
|
5
|
Limiting factors in Escherichia coli fed-batch production of recombinant proteins.
|
Biotechnol Bioeng
|
2003
|
1.31
|
6
|
A novel monothiol glutaredoxin (Grx4) from Escherichia coli can serve as a substrate for thioredoxin reductase.
|
J Biol Chem
|
2005
|
1.19
|
7
|
Transient increase of ATP as a response to temperature up-shift in Escherichia coli.
|
Microb Cell Fact
|
2005
|
1.18
|
8
|
Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction.
|
Microb Cell Fact
|
2007
|
1.12
|
9
|
Novel approach of high cell density recombinant bioprocess development: optimisation and scale-up from microliter to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures.
|
Microb Cell Fact
|
2010
|
1.11
|
10
|
A new wireless system for decentralised measurement of physiological parameters from shake flasks.
|
Microb Cell Fact
|
2006
|
1.11
|
11
|
Norvaline is accumulated after a down-shift of oxygen in Escherichia coli W3110.
|
Microb Cell Fact
|
2008
|
1.08
|
12
|
A role for bacteriophage T4 rI gene function in the control of phage development during pseudolysogeny and in slowly growing host cells.
|
Res Microbiol
|
2003
|
1.06
|
13
|
Pseudomonas fluorescens biofilms subjected to phage phiIBB-PF7A.
|
BMC Biotechnol
|
2008
|
1.04
|
14
|
Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system.
|
BMC Biotechnol
|
2008
|
1.03
|
15
|
The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies.
|
Microb Cell Fact
|
2005
|
1.02
|
16
|
Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates.
|
Microb Cell Fact
|
2003
|
1.02
|
17
|
High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor.
|
Microb Cell Fact
|
2010
|
0.99
|
18
|
High cell density media for Escherichia coli are generally designed for aerobic cultivations - consequences for large-scale bioprocesses and shake flask cultures.
|
Microb Cell Fact
|
2008
|
0.99
|
19
|
Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures.
|
Microb Cell Fact
|
2011
|
0.98
|
20
|
Octaketide-producing type III polyketide synthase from Hypericum perforatum is expressed in dark glands accumulating hypericins.
|
FEBS J
|
2008
|
0.95
|
21
|
Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracycline.
|
J Biotechnol
|
2006
|
0.95
|
22
|
16S rRNA targeted sandwich hybridization method for direct quantification of mycobacteria in soils.
|
J Microbiol Methods
|
2006
|
0.93
|
23
|
Functional role of the conserved active site proline of triosephosphate isomerase.
|
Biochemistry
|
2006
|
0.93
|
24
|
Effective inhibition of lytic development of bacteriophages lambda, P1 and T4 by starvation of their host, Escherichia coli.
|
BMC Biotechnol
|
2007
|
0.92
|
25
|
Bacteriophage contamination: is there a simple method to reduce its deleterious effects in laboratory cultures and biotechnological factories?
|
J Appl Genet
|
2004
|
0.91
|
26
|
Phage control of dual species biofilms of Pseudomonas fluorescens and Staphylococcus lentus.
|
Biofouling
|
2010
|
0.91
|
27
|
Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli.
|
Microb Cell Fact
|
2011
|
0.91
|
28
|
Quality control of inclusion bodies in Escherichia coli.
|
Microb Cell Fact
|
2010
|
0.90
|
29
|
Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures.
|
Microb Cell Fact
|
2013
|
0.90
|
30
|
Enhanced plasmid production in miniaturized high-cell-density cultures of Escherichia coli supported with perfluorinated oxygen carrier.
|
Bioprocess Biosyst Eng
|
2012
|
0.89
|
31
|
Response of Corynebacterium glutamicum exposed to oscillating cultivation conditions in a two- and a novel three-compartment scale-down bioreactor.
|
Biotechnol Bioeng
|
2015
|
0.88
|
32
|
Glucose-limited high cell density cultivations from small to pilot plant scale using an enzyme-controlled glucose delivery system.
|
N Biotechnol
|
2011
|
0.88
|
33
|
Reconstituted biosynthesis of the nonribosomal macrolactone antibiotic valinomycin in Escherichia coli.
|
ACS Synth Biol
|
2013
|
0.88
|
34
|
Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli.
|
Microb Cell Fact
|
2003
|
0.88
|
35
|
Antisense RNA based down-regulation of RNaseE in E. coli.
|
Microb Cell Fact
|
2006
|
0.87
|
36
|
Heterologous production of active ribonuclease inhibitor in Escherichia coli by redox state control and chaperonin coexpression.
|
Microb Cell Fact
|
2011
|
0.86
|
37
|
Complete genome sequence of the lytic Pseudomonas fluorescens phage ϕIBB-PF7A.
|
Virol J
|
2011
|
0.86
|
38
|
High-level production of human collagen prolyl 4-hydroxylase in Escherichia coli.
|
Matrix Biol
|
2004
|
0.85
|
39
|
High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer.
|
Microb Cell Fact
|
2011
|
0.85
|
40
|
Sandwich hybridization assay for sensitive detection of dynamic changes in mRNA transcript levels in crude Escherichia coli cell extracts in response to copper ions.
|
Appl Environ Microbiol
|
2008
|
0.84
|
41
|
Parallel production and verification of protein products using a novel high-throughput screening method.
|
Biotechnol J
|
2011
|
0.84
|
42
|
Physiology of resistant Deinococcus geothermalis bacterium aerobically cultivated in low-manganese medium.
|
J Bacteriol
|
2012
|
0.83
|
43
|
Expression of Escherichia coli glutaredoxin 2 is mainly regulated by ppGpp and sigmaS.
|
J Biol Chem
|
2002
|
0.82
|
44
|
Fed-batch process for the psychrotolerant marine bacterium Pseudoalteromonas haloplanktis.
|
Microb Cell Fact
|
2010
|
0.82
|
45
|
Direct and indirect use of GFP whole cell biosensors for the assessment of bioprocess performances: design of milliliter scale-down bioreactors.
|
Biotechnol Prog
|
2012
|
0.82
|
46
|
Single-chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre-induction glycerol feeding.
|
Biotechnol J
|
2011
|
0.81
|
47
|
Two-dimensional proteome reference map for the radiation-resistant bacterium Deinococcus geothermalis.
|
Proteomics
|
2010
|
0.81
|
48
|
Enhanced production of the nonribosomal peptide antibiotic valinomycin in Escherichia coli through small-scale high cell density fed-batch cultivation.
|
Appl Microbiol Biotechnol
|
2013
|
0.81
|
49
|
Two-compartment method for determination of the oxygen transfer rate with electrochemical sensors based on sulfite oxidation.
|
Biotechnol J
|
2011
|
0.81
|
50
|
Crystallographic binding studies with an engineered monomeric variant of triosephosphate isomerase.
|
Acta Crystallogr D Biol Crystallogr
|
2010
|
0.80
|
51
|
A two-compartment bioreactor system made of commercial parts for bioprocess scale-down studies: impact of oscillations on Bacillus subtilis fed-batch cultivations.
|
Biotechnol J
|
2011
|
0.80
|
52
|
Sensitive genus-specific detection of Legionella by a 16S rRNA based sandwich hybridization assay.
|
J Microbiol Methods
|
2005
|
0.80
|
53
|
Assessment of robustness against dissolved oxygen/substrate oscillations for C. glutamicum DM1933 in two-compartment bioreactor.
|
Bioprocess Biosyst Eng
|
2013
|
0.79
|
54
|
Polyamine metabolism during exponential growth transition in Scots pine embryogenic cell culture.
|
Tree Physiol
|
2012
|
0.79
|
55
|
Isolation and genotype-dependent, organ-specific expression analysis of a Rhodiola rosea cDNA encoding tyrosine decarboxylase.
|
J Plant Physiol
|
2009
|
0.79
|
56
|
Murine Wnt-1 with an internal c-myc tag recombinantly produced in Escherichia coli can induce intracellular signaling of the canonical Wnt pathway in eukaryotic cells.
|
J Biol Chem
|
2004
|
0.79
|
57
|
Small-scale slow glucose feed cultivation of Pichia pastoris without repression of AOX1 promoter: towards high throughput cultivations.
|
Bioprocess Biosyst Eng
|
2013
|
0.79
|
58
|
Process inhomogeneity leads to rapid side product turnover in cultivation of Corynebacterium glutamicum.
|
Microb Cell Fact
|
2014
|
0.78
|
59
|
Efficient lactic acid production from high salt containing dairy by-products by Lactobacillus salivarius ssp. salicinius with pre-treatment by proteolytic microorganisms.
|
J Biotechnol
|
2005
|
0.78
|
60
|
Mini-scale cultivation method enables expeditious plasmid production in Escherichia coli.
|
Biotechnol J
|
2014
|
0.78
|
61
|
Analytical biotechnology: from single molecule and single cell analyses to population dynamics of metabolites and cells.
|
Curr Opin Biotechnol
|
2010
|
0.77
|
62
|
High-temperature cultivation and 5' mRNA optimization are key factors for the efficient overexpression of thermostable Deinococcus geothermalis purine nucleoside phosphorylase in Escherichia coli.
|
J Biotechnol
|
2011
|
0.77
|
63
|
Use of slow glucose feeding as supporting carbon source in lactose autoinduction medium improves the robustness of protein expression at different aeration conditions.
|
Protein Expr Purif
|
2013
|
0.77
|
64
|
Recombinant protein production: a comparative view on host physiology (Laupheim, Germany, March 2013).
|
N Biotechnol
|
2013
|
0.77
|
65
|
RNA-based sandwich hybridisation method for detection of lactic acid bacteria in brewery samples.
|
J Microbiol Methods
|
2006
|
0.77
|
66
|
Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM.
|
Acta Crystallogr D Biol Crystallogr
|
2008
|
0.77
|
67
|
Modelling of translation of human protein disulfide isomerase in Escherichia coli-A case study of gene optimisation.
|
J Biotechnol
|
2005
|
0.76
|
68
|
Recombinant purine nucleoside phosphorylases from thermophiles: preparation, properties and activity towards purine and pyrimidine nucleosides.
|
FEBS J
|
2013
|
0.76
|
69
|
Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties.
|
Protein Eng Des Sel
|
2008
|
0.76
|
70
|
Enzyme-based glucose delivery: a possible tool for biosorbent preparation for heavy metal removal from polluted environments.
|
Bioprocess Biosyst Eng
|
2013
|
0.75
|
71
|
Modification of buffered peptone water for improved recovery of heat-injured Salmonella Typhimurium.
|
J Food Sci
|
2011
|
0.75
|
72
|
Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase.
|
Microb Cell Fact
|
2010
|
0.75
|
73
|
Potentiality of using microbial biosensors for the detection of substrate heterogeneities and the assessment of microbial viability in industrial bioreactors: a complete set of experiments in chemostat and scale down reactors, and elaboration of a mini scale-down platform.
|
Commun Agric Appl Biol Sci
|
2012
|
0.75
|
74
|
LC/MS/MS identification of glycosides produced by biotransformation of cinnamyl alcohol in Rhodiola rosea compact callus aggregates.
|
Biomed Chromatogr
|
2004
|
0.75
|
75
|
Cultivation of marine microorganisms in single-use systems.
|
Adv Biochem Eng Biotechnol
|
2014
|
0.75
|
76
|
The influence of P. fluorescens cell morphology on the lytic performance and production of phage φIBB-PF7A.
|
Curr Microbiol
|
2011
|
0.75
|
77
|
Psychoanalytic reflections through the prism of September 11, 2001.
|
Psychoanal Study Child
|
2011
|
0.75
|
78
|
Towards faster bioprocess development.
|
Biotechnol J
|
2011
|
0.75
|
79
|
Change of extracellular cAMP concentration is a sensitive reporter for bacterial fitness in high-cell-density cultures of Escherichia coli.
|
Biotechnol Bioeng
|
2004
|
0.75
|